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Biomedical subjects

I B Sorokulova

Publications and source records attributed to I B Sorokulova.

At least 19 recordsLinked to original sources
PubMed2006

[A study of the safety of probiotic bacilli].

The aim of the study was to evaluate the biological safety of a range of probiotic bacilli, ingredients of the commercial preparations biosporin, bactisubtil, and irilis; their biological effects were scrutinized in an animal experiment. The study demonstrated that the strains B. subtilis 3, 07, and B. licheniformis 31, 09, ingredients of biosporin and irilis, unlike B. cereus IP 5832, an ingredient of bactisubtil, were not toxic, toxigenic, or virulent, and did not impair the antitoxic hepatic function, which allows considering them to be safe.

Animals↗
PubMed2005

[Pre-clinical trials of new spore probiotics].

The paper presents the results of a pre-clinical trial of Bacillus bacterial strains that are ingredients of biosporin, bisporin, and irilis, new Russian probiotic preparations. The study included determination of the cultural and biochemical properties of the probiotic strains, their antagonistic activity, adhesion ability, resistance to various biological liquids, and the length of their persistence in mouse alimentary tract. The listed properties were compared with those of foreign commercial preparations, bactisubtil and enterogermin. The study demonstrated high antagonistic activity of biosporin, bisporin, and irilis against many bacteria of different taxonomic groups, and Candidae. Besides this, the study showed that, unlike similar foreign preparations, the Russian ones were characterized by relatively low adhesive ability, short-term persistence, and absence of virulence factors. According to the results of the study, the most prospective probiotic strains for further clinical application are B. subtilis VKPM B-8611 and B. licheniformis VKPM B-8610, an irilis ingredient.

Animals↗
PubMed2003

Genetic diversity and involvement in bread spoilage of Bacillus strains isolated from flour and ropy bread.

AIMS: To study Bacillus contamination of wheat flour and ropy bread, to analyse genetic diversity of isolated strains and to evaluate the ability of these strains to produce ropy bread. METHODS AND RESULTS: Classical and molecular methods [16S rDNA sequencing and random amplified polymorphic DNA (RAPD)-PCR] were used to identify and type-isolated strains. The predominant species isolated were Bacillus subtilis and B. licheniformis. RAPD analysis demonstrated that the same sample may harbor different strains. Ten of 15 strains of B. subtilis and four of six strains of B. licheniformis were able to cause rope spoilage of the laboratory-baked bread. CONCLUSION: RAPD typing can be useful in the tracking of Bacillus strains during bakery processing and in the understanding of the role of different Bacillus strains in the rope spoilage of bread. SIGNIFICANCE AND IMPACT OF THE STUDY: The results indicate the variability of Bacillus strains isolated from flour and responsible for rope spoilage of bread.

Bacillus↗
PubMed2001

Simplified technique for identification of the aerobic spore-forming bacteria by phenotype.

The use of modern research approaches of genetics, biochemistry and molecular biology has led to progress in bacterial taxonomy. Systematic study of the aerobic spore-forming bacteria has resulted in the realignment of the genus Bacillus into several new genera. In the meantime, the identification process has become more difficult for the non-specialist in Bacillus taxonomy. This paper presents a key for the simplified phenotypic identification of the mesophilic, aerobic, spore-forming bacteria belonging to the genera Bacillus, Paenibacillus, Brevibacillus, Aneurinibacillus, Geobacillus and Virgibacillus. A total of 81 species were included and 115 morphological and physiological tests were analysed for their discriminative efficiency. This key is practical for rough but quick identification of aerobic spore-forming bacteria isolated from nature. Such preliminary identification will be helpful for the selection of reference strains and methods for more precise identification using the newest techniques. The reliability of the proposed identification key was tested on 100 cultures from the Ukrainian Collection of Microorganisms. The developed identification key is represented in interactive mode on a website (http://www/imv.kiev.ua/key/).

Bacillaceae↗
PubMed2001

In vitro anti-Helicobacter pylori activity of the probiotic strain Bacillus subtilis 3 is due to secretion of antibiotics.

A limited number of antibiotics can be used against Helicobacter pylori infection, and resistance jeopardizes the success of treatment. Therefore, a search for new agents is warranted. The use of probiotics to enhance gastrointestinal health has been proposed for many years, but the scientific basis of the prophylactic and therapeutic actions of probiotics has not yet been clearly delineated. Probiotic strain Bacillus subtilis 3, whose safety has previously been demonstrated, is known to have antagonistic properties against species of the family Enterobacteriaceae. In the present study, it was also found to inhibit H. pylori. The anti-H. pylori activity present in the cell-free supernatant was not related to pH or organic acid concentration. It was heat stable and protease insensitive. At least two antibiotics, detected by thin-layer chromatography (R(f) values, 0.47 and 0.85, respectively) and confirmed by high-performance liquid chromatographic analysis, were found to be responsible for this anti-H. pylori activity. All H. pylori strains tested were sensitive to both compounds. One of these compounds was identified as amicoumacin A, an antibiotic with anti-inflammatory properties. MICs for H. pylori determined in solid and liquid media ranged between 1.7 and 6.8 microg/ml and 0.75 and 2.5 microg/ml, respectively. The underestimation of MICs determined in solid medium may be due to physicochemical instability of the antibiotic under these test conditions. An additive effect between amicoumacin A and the nonamicoumacin antibiotic against H. pylori was demonstrated.

Anti-Bacterial Agents↗
PubMed1998

[Effect of probiotics from bacilli on macrophage functional activity].

The influence of new probiotics from bacilli i.e. biosporin and subalin on the functional activity of murine peritoneal macrophages was studied. After a single oral administration of the probiotics the maximum activation of the macrophages was observed in 4 hours. The activation level depended on both the dose and the probiotic. The highest value of the stimulation index was recorded with subalin. Analogous regularities were stated with parenteral administration of the probiotics. Some mechanisms of activation of peritoneal macrophages after oral administration of probiotics from live microbial cultures are discussed.

Administration, Oral↗
PubMed1997

[Recombinant probiotics: problems and prospects of their use for medicine and veterinary practice].

Approaches to designing a new probiotic class based on recombinant strains of bacteria that produce the predetermined therapeutic proteins are dealt with. The prospects of the approach are shown via studies of the biological properties of Bacillus subtilis 2335 strain transformed by the plasmid encoding the synthesis of human interferon alpha-2. The recombinant strain was demonstrated to preserve the high antagonistic activity of the parent culture (the bases of the probiotic biosporine) and to acquire marked antiviral properties due to interferon synthesis. The antiviral activity of the designed strain was shown by in vitro and in vivo experiments on experimental viral infections. By using this strain, the authors designed the new probiotic subaline, a promising biological agent for medicine and veterinary practice. Subalin has a number of advantages: it combines antibacterial and antiviral properties, is easy to use and prepare.

Animals↗
PubMed1996

[Outlook for using bacteria of the genus Bacillus for the design of new biopreparations].

The data evident of the promising use of Bacillus as a basis of new probiotics are presented. The criteria of the Bacillus screening among the other representatives of the exogenic microflora provided the design of a probiotic, named biosporin, which markedly differed from the other biological preparations based on aerobic sporulating bacteria. Biosporin proved to be an efficient agent in the treatment of acute intestinal infection due to pathogenic and opportunistic bacteria as well as in the treatment of dysbacteriosis of various etiology. A principally new probiotic, named subalin, with antibacterial and antiviral properties was designed on the basis of biosporin by the gene engineering. The data on the promising use of Bacillus in the composition of complex probiotics are also presented.

Anti-Bacterial Agents↗
PubMed1994

[Susceptibility to antimicrobial drugs of strains of bacilli used as a basis for various probiotics].

Five strains of the genus Bacillus used as components of biological preparations i.e. bacterin SL, biosporin, bactisubtil and subalin were tested for their susceptibility to 38 antimicrobial agents, The strains were simultaneously resistant to 5 antibiotics: astreonam, colistin, penicillin, ceftizoxime and cefuroxime. High susceptibility of the strains to some penicillins, cephalosporins and aminoglycosides was recorded. The gene engineered strain B. subtilis 2335(105) marked by the kanamycin resistance gene showed cross resistance to amikacin and tobramycin. Trimetoprim, sulfotrimetoprim and norfloxacin proved to be active against the strains.

Anti-Bacterial Agents↗
PubMed1989

[New producers of site-specific endonucleases from microorganisms of the Bacillus genus].

52 strains of Bacillus generum have been tested for production of site-specific endonucleases. The sequence recognized by the enzyme was determined for 23 enzymes, the cleavage site inside the sequence was determined for 5 enzymes. All the enzymes under study were found to be isomers of the known enzymes. The selected strains are peculiar for the high level of site-specific endonucleases content and may be used as producents of the enzymes.

Bacillus↗
PubMed1984

Aerobic spore-forming bacteria as aetiological factors of bacteriaemia.

The paper presents data from the literature and results of the authors' own studies on the aetiological role of aerobic spore-forming bacteria in the development of bacteriaemia in man. Special attention is paid to various diseases caused by micro-organisms of the genus Bacillus. The importance of species-specific identification of bacilli isolated from blood and comprehensive study of pathogenicity of these bacteria is discussed.

Adult↗
PubMedDate not supplied

[The identification of freshly isolated strains, the causative agents of human candidiasis and the search for effective antifungal probiotics].

The species variety of yeasts of the genus Candida in children and patients with AIDS has been investigated. Composition of the studied species was found to be similar in newborns and in women with candidiasis. In order to create new probiotics with antifungal properties, the cluster analysis of antagonistic activity of some Bacillus strains against Enterobacteriaceae, Candida, normoflora and food-fermenting lactobacteria has been conducted. There were selected the combinations of some Bacillus strains, which effectively inhibited pathogenic and opportunistic microorganisms and at the same time had no effect on intestinal normoflora.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

[The monosaccharide composition of the glycocalyx in Mollicutes and phylogenetically related bacteria in the genus Bacillus].

A number of monosaccharides composing the glycocalix carbohydrates of some Mollicutes (Mycoplasma pneumoniae FH, M. hominis PG21, M. fermentans PG18, Acholeplasma laidlawii PG8, A. laidlawii var. granulum st. 118) and phylogenetically related bacteria from the genus Bacillus (Bacillus cereus 89, B. cereus DM423, B. subtilis 1/2, B. licheniformis 31, B. licheniformis 49) were detected using plant lectins of definite carbohydrate specificity labelled by colloid gold. The structure of extracellular glycopolymers of microorganisms was discussed concerning their role in the adhesion process and their specializations in colonization of the appropriate organs and tissues of macroorganisms as well as the action of probiotics on the basis of bacteria from the genus Bacillus. It was marked, that the mollicute cells typically interacted with all vegetable lectins tested while bacilli studied bind certain lectins only. The surface carbohydrates prevailing in monosaccharides from tested one for superficial structures of Bacillus pathogenic strains were: sialic acid, beta-D-galactose and alpha-N-acetyl-D-galactosamine, while L-fucose and alpha-D-glucose were observed in insignificant quantity or were absent. The nonpathogenic Bacillus strains being a basis of biopreparations were distinguished by the insignificant amount of sialic acid and other monosaccharides in the composition of carbohydrates of their glycocalix. Thus, as a result of research it was confirmed, that the superficial glycopolymers of Mollicutes and some related bacilli were alike as to presence of some monosaccharides that depended on taxonomic position of microorganisms and their biological activity.

Bacillus↗
PubMedDate not supplied

[The effect of the cultivation conditions on the properties of bacilli comprising the basis of probiotics].

Such biological factors as bile, gastric juice, blood serum, amino acids and pH of the medium have been studied for their effect on the growth intensity and antagonistic activity of bacilli being the basis of biosporin and subalin. It has been established that pH of the medium within 7.0-9.0 as well as certain concentrations of the gastric juice, bile, blood serum and most of amino acids did not affect the growth of Bacillus subtilis 3 and Bacillus subtilis 2335/105. A regular decrease of growth intensity in bacilli under the increase of the gastric juice concentration or decrease of pH of the medium to 5.0-2.0 is registered. The mentioned biological factors affect differently the antagonistic properties of the studied cultures.

Antibiosis↗
PubMedDate not supplied

[The effect of antibiotic preparations and their combinations with probiotics on the intestinal microflora of mice].

Antibiotic drugs (biseptol, polymyxin, canamycin) as well as their combinations with probiotics biosporin and subalin have been studied for their effect on mice intestine microflora. It has been established that under peroral administration of the above drugs dysbacteriosis of the 2-3rd degrees arises in animals; under their combined use with probiotics one can prevent the development of dysbacteriosis to considerable extent. It has been shown that dysbacteriosis of the 2-3rd degrees can be liquidated for 5-7 days, using probiotics biosporin and subalin. No self-recovery of normal intestinal microbiocenosis is observed in this period. Inconsiderable variations in the composition of normoflora under combined use of antibiotics with probiotics are renewed on the 3-5th day after stopping the drugs intake.

Animals↗
PubMedDate not supplied

[Bacteria of Bacillus species--prospective source for biologically active substances].

The present data from literature on bio-active substances produced by aerobic sporulating bacteria of Bacillus genus are presented. Problems of antibiotics synthesis by bacilli are considered. The antibiotics under consideration are both well-known and new ones--biosurfactants characterized by the surface-active properties. Enzymic activity of Bacillus genus bacteria has been characterized. Special attention was given to the analysis of enzymes, which could play a positive part in the digestion processes. Data are presented which evidence that the bacilli produce the substances, which affect essentially the immune reactivity of the macroorganism.

Anti-Bacterial Agents↗
PubMedDate not supplied

[Experimental evaluation of the biological safety of gene-engineered bacteria using a model strain Bacillus subtilis interferon-producing strain].

The in vitro and in vivo evaluation of the biological and ecological safety of genetically modified bacteria (GMB) was carried out on B. subtilis recombinant strain 2335/105, capable of producing human interferon alpha-2, used as experimental model. As shown in this investigation made with the use of bacteriological analysis and polymerase chain reaction, the oral administration of GMB to calves, chickens and white mice produced no disturbances in the microbial ecology of the gastrointestinal tract of warm-blooded animals and did not lead to the appearance of spontaneous transformants. The present work is the first experimental evaluation of the biological safety of genetically modified microorganisms, used as the component of Subalin, a probiotic preparation intended for use in veterinary practice.

Animals↗