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Biomedical subjects

I Aguilar

Publications and source records attributed to I Aguilar.

27 records · Page 2Linked to original sources
PubMed1993

Preparación toxoide a partir de la fracción hemorrágica del veneno de Bothrops asper (serpiente de América Central y del Sur) (Toxoid preparation from hemorrhagic fraction of the venom from Bothrops asper (snake from Central and South America).

A technique is described for preparing a toxoid from the hemorrhagic fraction of the Bothrops asper venom. This method conserves a high degree of immunogenicity although it eliminates lethal effects. None of the animals vaccinated with the toxoid from this fraction had hemorrhagic lesions after they were injected the venom from the hemorrhagic fraction.

Animals↗
PubMed1992

Secretion of macrophage fusion factor (MFF) by schistosome egg granulomas maintained in vitro.

The schistosome granuloma cultured in vitro under antigenic specific and unspecific stimulation releases macrophage fusion factor (MFF) into the medium, adjusting the production dynamics to the spontaneous modulation model already described. These findings demonstrate the feasibility of studying granulomatous response in mammalian schistosomiasis utilizing an in vitro model of lymphokine granuloma secretion.

Animals↗
PubMedDate not supplied

Análisis clínico y epidemiológico de los accidentes por mordeduras de serpientes del género Bothrops en Venezuela [A clinical and epidemiological analysis of accidental bites by snakes of the genus Bothrops in Venezuela].

Clinical register of 60 patients bitten by Bothrops snake who assisted at Leopoldo Manrique Hospital and the Institute of Tropical Medicine (HLM-IMT) in Caracas during 1996-1997 were analysed. The accident was more frequent in males (45/75%). In 32 cases (53.3%) the snake was classified and 26 were Bothrops lanceolatus, 4 Bothrops venezuelensis and 2 Bothrops atrox. Anatomic regions more frequent bitten were superior members (40/66.6%): hands (36/60%), forearm (2/3.3%), elbow (1/1.6%) and arm (1/1.6%). On inferior members (20/33.3%): legs (6/10%), feet (10/16.7%), ankle (2/3.3%), and the hip (2:3.3%). The most frequent clinical manifestations in moderate and severe cases (33 patient) were pain (100%), oedema (98%), ecchymosis (76%), blisters (20%), necrosis (12%), abscess (6%) bleeding (19%), heart failure (1/1.6%), renal failure (1/1.6%). The blood clotting was evaluated in 60 (100%) cases and it was altered in 33 (55%) patients. No deaths were recorded.

Adolescent↗
PubMedDate not supplied

ELISA assays for the detection of Bothrops lanceolatus venom in envenomed patient plasmas.

A double antibody sandwich enzyme linked immunosorbant assay (ELISA) was carried out to detect Bothrops Ianceolatus venom in plasma from envenomed patients at various time intervals (0, 6, 12, 18 and 24 hrs). The test could detect Bothrops lanceolatus levels up to 12 ng/mL of envenomed patient plasmas. Elaboration of an easy, fast and species-diagnostic based on this ELISA technique useful to physicians is discussed.

Animals↗
PubMedDate not supplied

Natural resistance of opossum (Didelphis marsupialis) to the mapanare (Bothrops lanceolatus) snake venom.

The inactivation of local and general effects of the Mapanare (Bothrops lanceolatus) venom by Opossum's (Didelphis marsupialis) serum fractions was tested using an in vivo assay and an in vitro preincubation experiment. A serum fraction of the Opossum serum has been obtained by immunochemical purification. It is only present in opossum's protective opossum serum fraction (F-0.1).

Animals↗
PubMedDate not supplied

Antivenom activity of opossum (Didelphys marsupialis) serum fractions against Uracoan rattlesnake (Crotalus vegrandis Klauber, 1941) venom.

In this work, we have found strong evidence for the presence of an opossum serum which is highly proficient in inactivating the neurotoxic fractions of Uracoan rattlesnake (Crotalus vegrandis) venom. Analyses of strained electrophoretic patterns of SDS gels run in non-reducing conditions revealed a small group of antivenom proteins in 0.1 M DEAE cellulose fraction that was not found in 0.05 M, 0.02 M, 0.25 M and 0.3 M NaCl ionic strength. Neutralizing activities to mapanare (Bothrops lanceolatus) venom have been already described but this is the first time that opossum serum anticrotalus activity is found. In spite of having preliminary results, we wish to make the corresponding report, while we accomplish the purification of the neutralizing component. One protein isolated from opossum serum or a synthetic peptide based on the aforementioned protein would probably be very useful in medical management of Crotalus vegrandis accidents.

Animals↗