Simple and sensitive test for the determination of phenolic compounds in urine and its application to melanoma.
A simple procedure is outlined for the determination of phenolic compounds in urine. The method involves the diazotization of p-nitroaniline and subsequent coupling of the diazonium chloride with phenolic compounds. The diazo compound formed is determined by measuring the absorbance at 400 nm. The extinction coefficients for several compounds have been determined. Application of this method to determine the levels of phenolic compounds in urine in patients with melanoma showed that the assay may be of diagnostic value. The amounts of phenols in 24-hr urine samples from patients with melanoma, patients with various other diseases, and healthy individuals were determined. A statistical analysis of these results showed that the results for patients with melanoma were higher than those for healthy individuals and patients with other diseases at 1 percent probability level. A comparison of this test with three other methods (Thormählen test, ferric chloride test, and ferrocitrate test) for determination of melanogens showed that the diazo test is the most sensitive one for detecting melanoma.