Biomedical subjects

Hua Jiang

Publications and source records attributed to Hua Jiang.

At least 73 records · Page 4Linked to original sources

PubMed2005

[Preliminary study of VEGF and its receptor expression on childhood acute lymphoblastic leukemia and its relativity to clinical manifestations].

OBJECTIVE: To investigate the relationship between differential expression of VEGF and its receptors and clinical characteristics of childhood acute lymphoblastic leukemia (ALL). METHODS: Expressions of VEGF and its receptors (Flt-1, KDR) were assayed by ELISA and RT-PCR in healthy donors(20 cases), ALL patients in remission (20 cases), with low risk (29 cases) and with high risk (10 cases). The clinical data of all the patients and volunteers enrolled in this study were collected and analyzed according to the expression of VEGF and its receptors. RESULTS: The expressions of VEGF were (574.37 +/- 208.45) ng/L, (387.93 +/- 175.86) ng/L, (135.80 +/- 111.28) ng/L and (91.16 +/- 41.34) ng/L in patients with high risk, standard risk, in remission and healthy donors, respectively. Expression levels of VEGF receptors were downwards with risk grades. The clinical manifestations were also in accord with the expression levels of VEGF and its receptors. CONCLUSION: ALL patients with highly expressed VEGF and its receptors are usually with higher tumor burden, and refractory treatment. Detection of VEGF and its receptors might be one of prognostic marker for ALL treatment.

Adolescent↗

PubMed2004

Strained aromatic oligoamide macrocycles as new molecular clips.

Can one join both ends of a helix? A helical aromatic oligoamide was macrocyclized into a saddle-shaped bifunctional clip molecule that self-assembles into discrete circular dodecamers in the solid state and shows great potential for binding aromatic acid guests in solution. The cyclization step requires that the helix is only partly denatured in the reaction medium.

Journal Article↗

PubMed2004

Expeditious assembly of mesoscopic metallocycles.

Very large chiral metallocycles with tunable cavities in the range of 0.9-22 nm were efficiently assembled from the requisite metal- and ligand-terminated oligomers that are built from the 2,2'-diacetoxy-1,1'-binaphthyl-3,3'-bis(ethyne) bridging ligand and trans-Pt(PEt3)2 metal connector. These unprecedented nanoscopic and mesoscopic molecular metallocycles have been characterized with 1H, 13C{1H}, and 31P{1H} NMR spectroscopy, microanalysis, IR, UV-vis, and circular dichroism spectroscopies, MALDI-TOF MS, and size exclusion chromatography (SEC). SEC results indicated that the metallocycles appear to be much more compact and rigid than the metal- and ligand-terminated oligomers. The present molecular metallocycles provide interesting building blocks for the construction of larger functional structures that cannot be accessed from a top-down approach.

Journal Article↗

PubMed2004

Electron transport in two-dimensional arrays of gold nanocrystals investigated by scanning electrochemical microscopy.

This article reports the use of the scanning electrochemical microscope (SECM) to investigate the electronic properties of Langmuir monolayers of alkane thiol protected gold nanocrystals (NCs). A substantial increase in monolayer conductivity upon mechanical compression of the Au NC monolayer is reported for the first time. This may be the room temperature signature of the insulator to metal transition previously reported for comparable silver NC monolayers. Factors influencing the conductivity of the monolayer NC array are discussed.

Journal Article↗

PubMed2004

Switching of chiral induction in helical aromatic oligoamides using solid state-solution state equilibrium.

The introduction of an R asymmetric center in an aromatic oligoamide that adopts stable helical conformations leads to a significant shift of the equilibrium between the right-handed and left-handed helices in solution: the R-P and R-M helices are diastereoisomers. However, these two species were found to cocrystallize in 1:1 proportions. Thus the chiral induction observed in solution is switched off in the solid state. This phenomenon represents an original and unexpected means to control handedness in helical oligomers.

Journal Article↗

PubMed2004

Cloning and analysis of human Apg16L.

Autophagy is an intracellular bulk degradation system, which delivers cytoplasmic components to the lysosome/vacuole. In yeast and mammalian cells, the Apg12-Apg5 conjugate, together with Apg16, form a multimeric complex, which plays an essential role in autopihageosome formation. By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA encoding a putative protein with 607 amino acid residues, which shows 90% identity and 93% similarity to mouse Apg16L. This protein, designated human Apg16L, contains a coiled-coil domain and a motif with seven WD repeats, which are also shared by mouse Apg16L. Database searching revealed that Apg16L is mapped to chromosome 2q37.1 and there exist at least four splice variants.

Alternative Splicing↗

PubMed2004

Development of genome-wide DNA polymorphism database for map-based cloning of rice genes.

DNA polymorphism is the basis to develop molecular markers that are widely used in genetic mapping today. A genome-wide rice (Oryza sativa) DNA polymorphism database has been constructed in this work using the genomes of Nipponbare, a cultivar of japonica, and 93-11, a cultivar of indica. This database contains 1,703,176 single nucleotide polymorphisms (SNPs) and 479,406 Insertion/Deletions (InDels), approximately one SNP every 268 bp and one InDel every 953 bp in rice genome. Both SNPs and InDels in the database were experimentally validated. Of 109 randomly selected SNPs, 107 SNPs (98.2%) are accurate. PCR analysis indicated that 90% (97 of 108) of InDels in the database could be used as molecular markers, and 68% to 89% of the 97 InDel markers have polymorphisms between other indica cultivars (Guang-lu-ai 4 and Long-te-pu B) and japonica cultivars (Zhong-hua 11 and 9522). This suggests that this database can be used not only for Nipponbare and 93-11, but also for other japonica and indica cultivars. While validating InDel polymorphisms in the database, a set of InDel markers with each chromosome 3 to 5 marker was developed. These markers are inexpensive and easy to use, and can be used for any combination of japonica and indica cultivars used in this work. This rice DNA polymorphism database will be a valuable resource and important tool for map-based cloning of rice gene, as well as in other various research on rice (http://shenghuan.shnu.edu.cn/ricemarker).

Base Sequence↗

PubMed2004

Angiotensin II type 1 receptor mRNA and its protein expression in human pancreatic cancer cell lines.

OBJECTIVE: The incidence of pancreatic cancer is increasing in China, and in many patients the surrounding lymphatics have already been invaded and there is blood-borne metastasis at the time of diagnosis. Additionally, pancreatic cancer is largely refractory to conventional therapies. Therefore, to improve its prognosis, it is important to resolve the problem of its growth. Angiotensin II type 1 receptor (AT1) stimulates the growth and angiogenesis of pancreatic cancer and a selective AT1 antagonist could inhibit these effects. The present study aimed to investigate the expression of AT1 in pancreatic cancer cell lines to provide the theoretical basis for its treatment. METHODS: The pancreatic cancer cell lines were SW1990, PaTu8988s and PANC-1. RT-PCR was used to detect the AT1 mRNA expression, and ABC immunocytochemical staining and SDS-PAGE were used to detect the expression of AT1 protein. RESULTS: Both AT1 mRNA and protein were expressed in all three cell lines. The AT1 protein was found on the cell membrane and in the cytoplasm of these cells. The AT1 protein (44 x 10(3)) was also demonstrated by SDS-PAGE. CONCLUSION: The results suggest that AT1 plays an important role in the growth of pancreatic cancer and its inhibition may be a therapeutic strategy.

Cell Line, Tumor↗

PubMed2004

[Complications related to permanent deep venous catheterization using dual lumen hemodialysis catheter].

OBJECTIVE: To observe the incidence of long-term venous indwelling catheter related complications in hemodialysis patients and to report our clinical approach to the complications. METHODS: A hundred eleven hemodialysis patients was prospectively studied from Jan. 2001 to Mar. 2003, in whom the venous indwelling catheter had been in place for more than 6 months. Catheter related complications were observed over a total period of 1090 patients months, with a mean period of 9.8 months (6 - 27 months). RESULTS: The incidence of hematoma was 8.1% (9/111), and that of catheter malposition was 4.5%. There was no hemothorax, pneumothorax, air embolism or veinous laceration. Six episodes of catheter related infection were observed during the observation, with an incidence of 5.4%; two cases with bacterial infection were successfully treated; while among the 4 cases with fungal infection, one was successfully treated and catheters were removed in other 3 cases for uncontrolled infection. The incidence of catheter thrombosis was 18.9% (21/111) and the catheter had been used for a mean period of 36 days (6 - 725 days) from placement to thrombosis. Catheter patency was restored in 95.2% (20/21) by urokinase infusion. Recurrent thrombosis occurred in 18 of 20 catheters (90.0%), of which 5 worked functionally following anti-platelet therapy and 13 had recurrent thrombosis. Following anticoagulation by warfarin, 9 became functional (69.2%), but the other 4 cases turned to other vascular access. Urokinase infusion followed by anticoagulation had prolonged the mean catheter life-time for 6 months (3 - 18 months). CONCLUSION: Cuffed dual lumen catheter as permanent access is safe and effective. Catheter thrombosis is the most common complication. Urokinase infusion followed by anticoagulation can significantly prolong the catheter life-time.

Adolescent↗

PubMed2004

[The dynamic expression of TNF-alpha mRNA of hypertrophic scars and its roles].

OBJECTIVE: To investigate the role of tumor necrosis factor alpha: TNF-alpha in the hypertrophic scar and find a valid way to treat the hypertrophic scars with gene therapy. METHODS: TNF-alpha mRNA expression was tested with a semiquantitative reverse polymerase chain reation (RT-PCR) method in the different period of a hypertrophic scar. RESULTS: There was a significant increase of TNF-alpha mRNA as the hypertrophic scar was getting mature (P < 0.01). However, the ratios of TNF-alpha mRNA was significantly in a lower level than the normal scar. CONCLUSIONS: The results suggest that TNF-alpha may play an important role in normal would healing, but the hypertrophic scar may result from the shortage of the TNF-alpha. This indicates that to increase the TNF-alpha with gene therapy may be a good way to treat the hyphertrophic scar.

Adult↗

PubMed2004

[Experimental study on p53 gene mutation in keloid fibroblasts].

OBJECTIVE: To explore the significance and the role of the p53 gene mutation in the exon 4 to 8 in keloid fibroblasts. METHODS: Tissue samples from twelve patients with keloid and twelve hyperplastic scar respectively were harvested for in vitro culture of fibroblasts, and normal skin samples from the same patients were employed as the control. Polymerase chain reaction-based single-strained conformational polymorphism (PCR-SSCP) and DNA sequencing were employed to detect p53 gene mutations of the fibroblasts. RESULTS: The points and frameshift mutations in the exon 4, 5, 6, 7 of p53 gene were identified in 9 of the 12 keloid tissue samples. No p53 gene mutation was detected in all hyperplastic scar and normal skin samples. CONCLUSION: p53 gene mutation might play an important role in the formation and development of keloids.

Female↗

PubMed2004

[Autogenous costal cartilage with Medpor: a compound auricular framework applied to one-stage total ear reconstruction].

OBJECTIVE: To introduce a compound external ear framework characterized by few complications, simplicity and very low rate of implant exposure or absorption. METHODS: A compound auricular framework, being composed of the ninth autogenous costal cartilage as ear rim and Medpor ear base, was used in one-stage total ear reconstruction. All patients were followed up postoperatively. RESULTS: Nine cases have been performed clinically using this compound framework and all of them achieved satisfactory efficacies. The reforged ears looked natural and achieved superior cosmesis, and none of them experienced ear rim fracture or implant exposure or distortion. CONCLUSION: This compound auricular framework has advantages of practicability, simplicity and minimal complications. It may be an ideal framework for the reconstruction of total external ear at present.

Adolescent↗

PubMed2004

[One-stage reconstruction of postburn whole auricle defect with MEDPOR ear scaffold covered with superficial temporoparietal fascia flap].

OBJECTIVE: To study the effect and the key points in the operative procedure of the one-stage reconstruction of postburn whole auricle defect with medpor car scaffold covered with superficial temporoparietal fascia (TPF) flap. METHODS: Medpor car scaffold was embedded under the superficial temporal (TFP) fascia. Razor-thin skin was grafted onto the surface of the fascia flap. RESULTS: Fifteen patients with postburn whole auricle defect were treated by one-stage reconstruction with Medpor ear scaffold during the last four years. It was successful in all the patients with satisfactory appearance of the reconstructed ears. CONCLUSION: Medpor possessed friendly biological compatibility. The reconstruction gave satisfactory results, and its advantages consisted of short operational time, easy manipulation, less injury to patients and good auricular contour.

Adolescent↗

PubMed2004

[The effects of extracts from Herba Lagopsis on lymph microcirculation of acute blood stasis rats].

OBJECTIVE: To observe the effects of the extracts from Herba Lagopsis (HLE) on lymph microcirculation of acute blood stasis rats. METHODS: The acute blood stasis model was made by iv dextran (10 ml/kg) within 1 min. After 6 minutes, HLE(1.0g/kg) was given intravenously to the treatment group and the same volume of NS was done in the normal control group. The changes of contractility of mesenteric micro-lymphatics (ML) was observed by using a vital microscope with TV recorder. RESULTS: HLE could markedly improve the ML spontaneous constraction frequency, lymphatics constractive activity (Index I), total lymphatics constractive activity (Index II) and lymphatics dynamics (L. D-Index) (P < 0.05 approximately 0.01). CONCLUSION: HLE played an important role in lymph microcirculation of acute blood stasis rats.

Acute Disease↗

PubMed2004

[Advances in phalloplasty].

The penis is an important genitourinary organ, whose deficiency not only causes physiological dysfunction but inflicts mental and psychological traumas to the patient. Ideal phalloplasty should address the following requirements: (1) It should give a good shape; (2) It should ensure appropriate size and sexual intercourse; (3) It should make the phallus sensitive to both touching and sexual desire; (4) The donor site morbidity should be inconspicuous. To satisfy the above-mentioned requirements, doctors both at home and abroad have made increasing efforts for the improvement of phalloplasty. Recent progress in phalloplasty is reviewed in this article.

Humans↗

PubMed2004

[Effect of expression of platelet-derived growth factor B gene on blood vessel reconstruction after tissue engineering skin grafting].

OBJECTIVE: To study the effect of PDGF on dermal blood vessel reconstruction by transplanted tissue-engineering skin containing PDGF-B gene to rats. METHODS: The recombined eukaryotic expression vector, pcDNA3.1-hPDGF-B, was constructed and transfected into fibroblasts mediated by LipofectAMINE. Keratinocytes + acellular dermal matrix (group A), keratinocytes + acellular dermal matrix + fibroblasts (group B), keratinocytes + acellular dermal matrix + fibroblasts with PDGF gene (group C) were recombined respectively, then transplanted them to rat dorsum and evaluated the reconstruction of blood vessels in the dermis after 2, 4, 6 week postoperation. RESULTS: In 2-4 weeks after skin grafting the vascularization rate in group C was higher than that of group B and group A. The vascularization rates in all groups had no significant differences in six weeks (P > 0.05). CONCLUSION: PDGF-B gene plays an important role in reconstruction of blood vessels in the dermis at early tissue-engineering skin grafting, which ensures the take of grafted tissue-engineering skin.

Acellular Dermis↗

PubMed2003

Self-assembly of chiral molecular polygons.

Treatment of 2,2'-diacetyl-1,1'-binaphthyl-6,6'-bis(ethyne), L-H2, with 1 equiv of trans-Pt(PEt3)2Cl2 led to a mixture of different sizes of chiral metallocycles [trans-(PEt3)2Pt(L)]n (n = 3-8, 1-6). Each of the chiral molecular polygons 1-6 was purified by silica gel column chromatography and characterized by 1H, 13C{1H}, and 31P{1H} NMR spectroscopy, MS, IR, UV-vis, and circular dichroism spectroscopies, and microanalysis. The presence of tunable cavities (1.4-4.3 nm) and chiral functionalities in these molecular polygons promises to make them excellent receptors for a variety of guests.

Journal Article↗

PubMed2003

Aromatic delta-peptides.

Oligoamides of 8-amino-2-carboxy-quinoline adopt a very stable helical conformation characterized in solution by 1H NMR and in the solid state by single-crystal X-ray diffraction. The helix comprises only 2.5 units per turn, which represents the highest curvature achieved by aromatic oligoamides until now. Monomers possessing alkoxy substituents in position 4 are easily available from 2-nitroaniline and dimethyl acetylenedicarboxylate.

Models, Molecular↗