Biomedical subjects

Hsin-Chieh Wu

Publications and source records attributed to Hsin-Chieh Wu.

3 recordsLinked to original sources

PubMed2005

Complete recovery time after exhaustion in high-intensity work.

This study was aimed to investigate complete recovery time (CRT) after exhaustion in high-intensity work. Twenty-four subjects were divided into two groups based on the cardiorespiratory capability index, which was measured in a maximum capacity test. Each subject then performed two cycling tests (at 60% and 70% maximum working capacity). The subject continued cycling until exhaustion in each test and then sat recovering until he/she no longer felt fatigue or until the oxygen uptake (VO2) and heart rate (HR) returned to their baselines, whichever was longer. The results indicated that HR required the longest time to recover and, consequently, HR data were adopted to set the CRT. The CRT was significantly correlated with the cardiorespiratory capability index and the relative workload indices: RVO2 and RHR. The RVO2 was the average elevation in VO2 during work from the resting level as a percentage of maximum VO2 reserve. The RHR's definition was similar to that of RVO2. Based on the obtained CRT-prediction model, the CRT for a high-cardiorespiratory-capability person was 20.8, 22.1, 23.4, and 24.7 min at 50%, 60%, 70%, and 80% RHR levels, respectively. These suggested CRT values should be increased by 10 min for a low-cardiorespiratory-capability person.

Adult↗

PubMed2003

Regulation of selenoprotein GPx4 expression and activity in human endothelial cells by fatty acids, cytokines and antioxidants.

Phospholipid hydroperoxide glutathione peroxidase (GPx4) is the only antioxidant enzyme known to directly reduce phospholipid hydroperoxides within membranes and lipoproteins, acting in conjunction with alpha-tocopherol to inhibit lipid peroxidation. Peroxidation of lipids has been implicated in a number of pathophysiological processes, including inflammation and atherogenesis. We investigated the relative positive and negative effects of specific polyunsaturated fatty acids (PUFAs) and inflammatory cytokines on the activity and gene expression of the selenium-dependant redox enzyme GPx4. In human umbilical vein endothelial cells (HUVEC), GPx4 mRNA levels and activity were increased optimally by 114 nM selenium (as sodium selenite). Docosahexaenoic acid (DHA) and conjugated linoleic acid (CLA) further increased mRNA levels whereas arachidonic acid (ARA) had no effect; enzyme activity was decreased by DHA, was unaffected by CLA or was increased by ARA. GPx4 protein levels increased with selenium, ARA and DHA addition but not with CLA. Interleukin-1beta (IL-1beta) increased GPx4 mRNA, protein and activity whereas TNFalpha at 1 ng/ml increased activity while at 3 ng/ml it reduced activity and mRNA. Conversely, alpha-tocopherol reduced mRNA levels without affecting activity. These results indicate that lipids, cytokines and antioxidants modulate GPx4 in a complex manner that in the presence of adequate selenium, may favour protection against potentially proatherogenic processes.

Antioxidants↗

PubMed2002

Relationship between maximum acceptable work time and physical workload.

For safe job design, it is necessary to know the maximum acceptable work time (MAWT) for a given workload. The aim was to establish the relationship between MAWT and physical workload. Cycling tests at six different work rates relative to personal maximum working capacity were performed by 12 young adults in the laboratory. The oxygen uptake (VO(2)) in the per cent maximum aerobic capacity (%VO(2max)), relative heart rate (RHR) and relative oxygen uptake (RVO(2)) were collected throughout the test. MAWT was determined by observing the heart rate data during the test. The results showed that MAWT was negatively correlated with %VO(2max), RHR and RVO(2) (p < 0.01). Three exponential regression models were obtained and all their R(2) > 0.80. These models suggest that long-hour shifts (> 10 h) should assign a lower work intensity than for an 8-h workday. It is also logical that the workload limit for a 4-h work shift could be set at about 10% VO(2max) higher than the suggested limit for an 8-h workday.

Adult↗