Biomedical subjects
Hongjin Duanmu
Publications and source records attributed to Hongjin Duanmu.
[Report on fourth national epidemiological sampling survey of tuberculosis].
OBJECTIVE: To investigate the epidemiological trend of tuberculosis, to evaluate the efficacy of control measures and to provide scientific basis for making National Tuberculosis Control Programme 2001 approximately 2010. METHODS: Tuberculin testing was carried out among 0 approximately 14 years old children; fluroscopy was carried out for >/= 15 years old population and children with >/= 10 mm reaction of tuberculin testing; chest X-ray film, sputum smear and culture were done for the patients of fluroscopy abnormal and suspects of tuberculosis symptom (persistent cough for 3 weeks or more); drug sensitivity test was done for the patients with culture positive; a retrospective study of tuberculosis mortality in 1999 was conducted at all investigation points; social economic study was done for the active pulmonary tuberculosis cases; the survey of tuberculosis infection rate for all population was carried out in 59 investigation points. RESULTS: The population actually examined in this survey numbered 365 097. The examination rate was more than 95%. The prevalence of active pulmonary tuberculosis was 367/100 000, the prevalence of smear positive pulmonary tuberculosis was 122/100 000 and the prevalence of bacteriological positive pulmonary tuberculosis was 160/100 000. In comparison with 1979, the annual reduction rates were 4.5% for the standardized prevalence of active pulmonary tuberculosis and 3.8% for the standardized prevalence of smear positive pulmonary tuberculosis. In comparison with 1990, the annual reduction rates were 5.4% and 3.2%, respectively. The smear positive prevalence standardized showed a 44.4% decrease in the regions of implementing project of Health V but only 12.3% decrease in the regions without the project. The prevalence in the west region was higher than national average prevalence. CONCLUSIONS: The epidemic of tuberculosis is still serious and prevalence decrease was slow. The governments at different levels must pay more attention to tuberculosis control programme, increase budget, implement DOTS strategy.
[IS6110-restriction fragment length polymorphism based DNA fingerprinting in molecular epidemiology of tuberculosis].
OBJECTIVE: To identify the IS6110-restriction fragment length polymorphism (RFLP) DNA fingerprinting patterns of some clinical isolates of Mycobacterium tuberculosis (MTB) isolated from Ningxia, Beijing and Shanghai in recent years, and to observe their epidemiological characteristics. METHODS: Chromosomal DNA of MTB was digested with endonuclease PvuII, then electrophoresed on agarose gel plate and transferred capillarilly to a nylon filter and hybridized with 245 bp fragment of IS6110 which labeled using random primer fluorescein labeling kit. The RFLP patterns of Southern hybridization were inspected autofluorographically and the chromosomal DNAs of MTB were thereby typed. RESULTS: Most of 103 isolates of MTB shared 8 approximately 21 copies. Some of them were clustered. Strains isolated from Ningxia and Beijing had similar DNA fingerprinting patterns. One zero copy strain and one single copy strain were found among isolates from Shanghai. CONCLUSIONS: IS6110-RFLP based typing is feasible for MTB molecular epidemiological study in China. Most of isolates of MTB show analyzable patterns. Isolates of MTB from Ningxia and Beijing have close genomic relation.