Biomedical subjects

Henrik Bruus

Publications and source records attributed to Henrik Bruus.

11 recordsLinked to original sources

PubMed2007

Quantitative characterization of magnetic separators: comparison of systems with and without integrated microfluidic mixers.

We present two new types of microfluidic passive magnetic bead separator systems as well as methods for performing quantitative characterizations of them. Both systems consist of a microfluidic channel with long rectangular magnetic elements of permalloy that are placed by the sides of the channel and magnetized by an external magnetic field. In one of the systems, a staggered herringbone microfluidic mixer is integrated in the channel. The characterization of the systems includes magnetic measurements of the capture-and-release efficiencies, estimates of distributions of captured beads in a channel from micrographs, and simulations and analytical models of bead trajectories, capture efficiencies, and capture distributions. We show that the efficiencies of both systems compare favorably to those in the literature. For the studied geometries, the mixer is demonstrated to increase the bead capture-and-release efficiency for a fixed flow rate by up to a factor of two. Moreover, high capture efficiencies can be achieved in the system with mixer at up to ten times higher flow rates than in the system without mixer.

Computer Simulation↗

PubMed2006

Universal dynamics in the onset of a Hagen-Poiseuille flow.

The dynamics in the onset of a Hagen-Poiseuille flow of an incompressible liquid in a channel of circular cross section is well-studied theoretically. We use an eigenfunction expansion in a Hilbert space formalism to generalize the results to channels of an arbitrary cross section. We find that the steady state is reached after a characteristic time scale tau=(A/P)2(1/nu), where A and P are the cross-sectional area and perimeter, respectively, and nu is the kinematic viscosity of the liquid. For the initial dynamics of the flow rate Q for t<<tau we find a universal linear dependence, Q(t)=Q(infinity) (alpha/C)(t/tau), where Q(infinity) is the asymptotic steady-state flow rate, alpha is the geometrical correction factor, and C=P2/A is the compactness parameter. For the long-time dynamics Q(t) approaches Q(infinity) exponentially on the time scale , but with a weakly geometry-dependent prefactor of order unity, determined by the lowest eigenvalue of the Helmholz equation.

Journal Article↗

PubMed2006

ac electrokinetic micropumps: the effect of geometrical confinement, Faradaic current injection, and nonlinear surface capacitance.

Recent experiments have demonstrated that ac electrokinetic micropumps permit integrable, local, and fast pumping (velocities approximately mm/s) with low driving voltage of a few volts only. However, they also displayed many quantitative and qualitative discrepancies with existing theories. We therefore extend the latter theories to account for three experimentally relevant effects: (i) vertical confinement of the pumping channel, (ii) Faradaic currents from electrochemical reactions at the electrodes, and (iii) nonlinear surface capacitance of the Debye layer. We report here that these effects indeed affect the pump performance in a way that we can rationalize by physical arguments.

Journal Article↗

PubMed2006

Universality in edge-source diffusion dynamics.

We show that in edge-source diffusion dynamics the integrated concentration N(t) has a universal dependence with a characteristic time scale tau=(A/P)2pi/(4D), where D is the diffusion constant while A and P are the cross-sectional area and perimeter of the domain, respectively. For the short-time dynamics we find a universal square-root asymptotic dependence N(t)=N0(sqrt)t/tau while in the long-time dynamics N(t) saturates exponentially at N0. The exponential saturation is a general feature while the associated coefficients are weakly geometry dependent.

Journal Article↗

PubMed2005

AC electroosmotic pump with bubble-free palladium electrodes and rectifying polymer membrane valves.

We present the design, test and theoretical analysis of a novel micropump. The purpose is to make a pump with large flow rate (approximately 10 microL min-1) and high pressure capacity (approximately 1 bar) powered by a low voltage DeltaV<30 V. The pump is operated in AC mode with an electroosmotic actuator in connection with a full wave rectifying valve system. Individual valves are based on a flexible membrane with a slit. Bubble-free palladium electrodes are implemented in order to increase the range of applications and reduce maintenance.

Electrodes↗

PubMed2005

Microfluidic capturing-dynamics of paramagnetic bead suspensions.

We study theoretically the capturing of paramagnetic beads by a magnetic field gradient in a microfluidic channel treating the beads as a continuum. Bead motion is affected by both fluidic and magnetic forces. The transfer of momentum from beads to the fluid creates an effective bead-bead interaction that greatly aids capturing. We demonstrate that for a given inlet flow speed a critical density of beads exists above which complete capturing takes place.

Magnetics↗

PubMed2005

Surface-directed capillary system; theory, experiments and applications.

We present a capillary flow system for liquid transport in microsystems. Our simple microfluidic system consists of two planar parallel surfaces, separated by spacers. One of the surfaces is entirely hydrophobic, the other mainly hydrophobic, but with hydrophilic pathways defined on it by photolithographic means. By controlling the wetting properties of the surfaces in this manner, the liquid can be confined to certain areas defined by the hydrophilic pathways. This technique eliminates the need for alignment of the two surfaces. Patterned plasma-polymerized hexafluoropropene constitutes the hydrophobic areas, whereas the untreated glass surface constitutes the hydrophilic pathways. We developed a theoretical model of the capillary flow and obtained analytical solutions which are in good agreement with the experimental results. The capillarity-driven microflow system was also used to pattern and immobilize biological material on planar substrates: well-defined 200 microm wide strips of human cells (HeLa) and fluorescence labelled proteins (fluorescein isothiocyanate-labelled bovine serum albumin, i.e., FITC-BSA) were fabricated using the capillary flow system presented here.

Adsorption↗

PubMed2005

Long-term stable electroosmotic pump with ion exchange membranes.

We present the design, fabrication and test of a novel inline frit-based electroosmotic (EO) pump with ion exchange membranes. The pump is more stable than previous types due to a new flow component that ensures a controlled width of the diffusion layer close to the ion exchange membranes. The pump casing is constructed in polymers while the EO active part, the frit, is made in a nanoporous silica. The pressure capability of the pump is Deltapm/DeltaV = 0.15 bar V(-1). The flow rate to current ratio is Qm/I = 6 microL min(-1) mA(-1). This translates to Deltapm = 4.5 bar and Qm = 6 microL min(-1) at DeltaV = 30 V. The pump has been tested with four different buffer concentrations. In order to investigate day-to-day reproducibility each Q-p pump characteristic has been recorded several times during hour-long operation runs under realistic operating conditions.

Journal Article↗

PubMed2005

Electrohydrodynamics of binary electrolytes driven by modulated surface potentials.

We study the electrohydrodynamics of the Debye screening layer that arises in an aqueous binary solution near a planar insulating wall when applying a spatially modulated ac voltage. Combining this with first order perturbation theory we establish the governing equations for the full nonequilibrium problem and obtain analytic solutions in the bulk for the pressure and velocity fields of the electrolyte and for the electric potential. We find good agreement between the numerics of the full problem and the analytics of the linear theory. Our work provides the theoretical foundations of circuit models discussed in the literature. The nonequilibrium approach also reveals unexpected high-frequency dynamics not predicted by circuit models.

Journal Article↗

PubMed2005

Reexamination of Hagen-Poiseuille flow: shape dependence of the hydraulic resistance in microchannels.

We consider pressure-driven, steady-state Poiseuille flow in straight channels with various cross-sectional shapes: elliptic, rectangular, triangular, and harmonic-perturbed circles. A given shape is characterized by its perimeter P and area A which are combined into the dimensionless compactness number C= P2/A, while the hydraulic resistance is characterized by the well-known dimensionless geometrical correction factor alpha. We find that alpha depends linearly on C, which points out C as a single dimensionless measure characterizing flow properties as well as the strength and effectiveness of surface-related phenomena central to lab-on-a-chip applications. This measure also provides a simple way to evaluate the hydraulic resistance for the various shapes.

Journal Article↗

PubMed2003

Polymer microfluidic chip for online monitoring of microarray hybridizations.

A disposable single use polymer microfluidics chip has been developed and manufactured by micro injection molding. The chip has the same outer dimensions as a standard microscope slide (25 x 76 x 1.1 mm) and is designed to be compatible with existing microscope slide handling equipment like microarray scanners. The chip contains an inlet, a 10 microL hybridization chamber capable of holding a 1000 spot array, a waste chamber and a vent to allow air to escape when sample is injected. The hybridization chamber ensures highly homogeneous hybridization conditions across the microarray. We describe the use of this chip in a flexible setup with fluorescence based detection, temperature control and liquid handling by computer controlled syringe pumps. The chip and the setup presented in this article provide a powerful tool for highly parallel studies of kinetics and thermodynamics of duplex formation in DNA microarrays. The experimental setup presented in this article enables the on-chip microarray to be hybridized and monitored at several different stringency conditions during a single assay. The performance of the chip and the setup is demonstrated by on-line measurements of a hybridization of a DNA target solution to a microarray. A presented numerical model indicates that the hybridization process in microfluidic hybridization assays is diffusion limited, due to the low values of the diffusion coefficients D of the DNA and RNA molecules involved.

Automation↗