Biomedical subjects

Hao Wu

Publications and source records attributed to Hao Wu.

131 records · Page 8Linked to original sources

PubMed2002

[Clinical characteristics and diagnosis of Pneumocystis carinii pneumonia (PCP) in patients with the acquired immunodeficiency syndrome (AIDS)].

OBJECTIVE: To explore the clinical characteristics and the diagnostic methods for PCP in patients with AIDS. METHODS: Twelve cases of AIDS associated PCP confirmed by sputum polymerase chain reaction (PCR) were analyzed. RESULTS: Tuberculosis was found in 4 cases and pneumonia in 1 case of PCP. Sputum PCR was positive in all the 12 cases, of them blood PCR was positive in 9, Gomori methenamine silver stain (GMS) positive in 5 and Giemsa stain positive in 6 cases. The CD(4)(+) lymphocyte count was (5 approximately 155) x 10(6)/L, with a mean of (51 +/- 48) x 10(6)/L; the number of CD(4)(+) cells less than 100 x 10(6)/L in 10 cases (83%) with 9 cases (75%) less than 50 x 10(6)/L. The CD(4)(+)/CD(8)(+) ratio was 0.01 approximately 0.29. CONCLUSIONS: PCP was common in patients with advanced AIDS and often co-infected with other opportunistic infections such as tuberculosis. A diagnosis of PCP should be considered if sputum PCR is positive and typical clinical manifestations are present in patients with AIDS.

AIDS-Related Opportunistic Infections↗

PubMed2002

[The alteration of inflammatory cytokine during acute pancreatitis].

OBJECTIVE: To observe the alteration of both inflammatory and anti-inflammatory cytokines during acute pancreatitis, and to investigate the effect of somatostatin on modulation of inflammatory and anti-inflammatory cytokines in experimental acute pancreatitis. METHODS: SD male rats were divided into 3 groups: group 1. the normal rats as control (n = 6); group 2, the rats with acute pancreatitis induced by transabdominal injection of 5% sodium cholate sulfur (at the volume of 1.0 ml/kg) into the parcreatic duct and not given drug treatment; group 3, the rats injected with stilamin 20 micrograms/kg, intravenously, 30 minutes after the successful induction of acute pancreatitis. The animals in groups 2 and 3 were killed at 2, 6 and 24 hours after operation. The blood samples were taken for measurement of IL-1, TNF alpha, IL-6 (by Bioassay) and IL-10, TNF-beta (by ELISA). The wet weight of pancreatic tissue and amylase were also determined. RESULTS: Serum IL-1, TNF alpha, IL-6, IL-10 and TGF-beta in control group were 0.56 +/- 0.06 ng/ml, 23.50 +/- 1.87 IU/ml, 69.0 +/- 6.40 IU/ml, 32.05 +/- 14.87 pg/ml and 66.4 +/- 13.20 pg/ml, respectively. After acute pancreatitis was induced, the serum level of these inflammation-concerned cytokines increased significantly in group 2 (P < 0.05). Serum IL-1, TNF alpha, IL-6, IL-10 and TGF-beta in group 2 at 24 hours after pancreatitis were 1.15 +/- 0.13 ng/ml, 55.33 +/- 12.79 IU/ml. 127.17 +/- 13.91 IU/ml, 68.13 +/- 19.90 pg/ml, and 103.77 +/- 28.95 pg/ml, respectively. After administration of somatostatin, the inflammation-concerned cytokines in group 3 were remarkably decreased (P < 0.05). Serum IL-1, TNF alpha, IL-6, IL-10 and TGF-beta in group 3 were 0.83 +/- 0.12 ng/ml, 33.00 +/- 7.40 IU/ml. 71.83 +/- 6.34 IU/ml, 42.2 +/- 14.55 pg/ml, and 45.98 +/- 18.10 pg/ml, respectively. The indeies of the severity of pancreatitis, such as amylase and the weight of pancreas alse improved in group 3. CONCLUSION: Both inflammatory and antiinflammatory cytokines increased remarkably in the rats with acute pancreatitis. This result indicates that there is a potential tendency of inflammatory response syndrome and compensatory anti-inflammatory response syndrome in the course of acute pancreatitis. Somatostatin can modulate the derangement of these cytokines in acute pancreatitis.

Acute Disease↗

PubMed2002

[Reinnervation of the posterior cricoarytenoid muscle by the phrenic nerve for bilateral vocal cord paralysis in humans].

OBJECTIVE: To reestablish the respiratory abduction of the paralyzed vocal cord through reinnervation of the posterior cricoarytenoid(PCA) muscle by the phrenic nerve in humans. METHODS: In six cases with bilateral recurrent laryngeal nerve paralysis, the phrenic nerve was anastomosed to the anterior branch of recurrent laryngeal nerve, while the adductor branch of recurrent laryngeal nerve was severed and its proximal end was implanted into the PCA muscle belly in one side, for the other side nerve-muscle pedicle technique was used. All cases had been subjected to preoperative and postoperative video laryngoscopy, stroboscopy, electromyography, voice recording and acoustic analysis. RESULTS: Among the 6 patients, it is observed in five cases' phrenic nerve reinnervation side the inspiratory abducent motion evidently recovered, and the abducent range was from 3 to 5 mm, While only slight abductent motion or no motion could be recorded on the other side reinnervated with nerve-muscle pedicle technique, and the vocal cord excursion on this side was less than 1 mm in all cases. It is because the glottis is broad enough for the patients to have daily activities without short of breath, so all of them were decannulated postoperatively. The reinnervated PCA muscle by the phrenic nerve showed typical inspiratory high frequency discharge with 100-200 ms delay as compared with the other side, indicating the phrenic motoneuron pattern. No long-term diaphragmatic paralysis and lesion of respiratory function was found. All cases' voice was not weakened, and no aspiration occurred. CONCLUSION: The phrenic reinnervation is feasible clinically for treating vocal cord paralysis, and it is found to be more effective for restoring inspiratory abducent function than the nerve-muscle pedicle technique.

Adult↗

PubMed2002

[The study of MRI examination of acoustic neuromas].

OBJECTIVE: To discuss the examination parameters of acoustic neuromas. METHOD: The MRI features of 20 lesions 20 patients with acoustic neuroma had been analyzed retrospectively. The examinations were performed by a 1.5 Tesla superconducting magnet system transaxial T1. T2-weighted imagines and coronal or sagittal T1. T2-weighted imagines were obtained in each cases. 20 patients were examined with Gd-DTPA. RESULT: 20 cases of acoustic neuroma were divided into three groups according to the tumor size: 1.1 approximately 2 cm (4 lesion) ;2. 2 approximately 4 cm (14 lesion); 3. over 4 cm (2 lesion). T1 weighted imagines shovos the equal or low signal;T2 weighted imagines shows the high signal. Low and high mixture signal will be shown when being hemorrhage or necrosis. CONCLUSION: Combining thin slice and Gd-DTPA in MRI examination was the best method to find acoustic neuroma. MRI not only can find small intracanalicular tumors, but also has advantages in demonstrating the formation of cyst and hemorrhage, the compression of adjacent anatomical structures and the tumor vessels.

Adult↗

PubMedDate not supplied

Localization and mRNA expression of somatostatin receptor subtypes in human prostatic tissue and prostate cancer cell lines.

Somatostatin (SST) plays an important regulatory role in the physiological control of various organs including the prostate. Somatostatin receptors (SSTRs) and SST analogs are potential targets for prostate cancer treatment, especially since it has been shown that SST analogues are clinically effective in the treatment of advanced prostate cancer. The presence of SST containing neuroendocrine (NE) cells in the epithelium of the human prostate and their suggested role in the paracrine regulation of this gland prompted us to study the potential expression of somatostatin receptors (SSTRs) in human prostatic tissue and prostate cancer cell lines. Using the reverse transcriptase polymerase chain reaction (RT-PCR), we found the SSTR subtypes 1-3 in stromal cells and in prostate cancer cell lines LNCaP, PC-3 and DU 145. Immunohistochemical analysis of 27 radical prostatectomy specimens demonstrated the presence of hSSTR1 in a subpopulation of cancerous and NE cells, whereas hSSTR2 was found in the stroma, peritumoral blood vessels and tumor cells. Receptor subtype 3 was demonstrated to be present on the cell membrane of BPH and malignant areas. A strong immunoreaction (IR) of hSSTR4 was found in tumor cells, as compared with a less intense IR in adjacent BPH areas. Somatostatin receptor subtype 5 was not detectable. Western blot analysis revealed immunoreactive bands of molecular weight between 44-60 kDa. In summary, the present study clearly demonstrates the presence of hSSTR1-3 in tumoral and nontumoral epithelial cells as well as in the stromal compartment, whereas hSSTR4 was found to be confined to epithelial cells, and SSTR5 was not detectable.

Adult↗