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Biomedical subjects

H de Barjac

Publications and source records attributed to H de Barjac.

At least 37 records · Page 2Linked to original sources
PubMed1976

[Biochemical determination of B. thuringiensis thermostabile, exotoxin, using the inhibition of bacterial RNA polymerases].

This method can determine amounts of exotoxin near 1 mug. Used on the autoclaved filtrates of cultures of B. thuringiensis var. thuringiensis (serotype 1 type-strain), it shows an excretion rate of 250 to 300 mug per millilitre. The results on different exotoxin-producer strains give a perfect parallelism with the results obtained by bio-assays on Insects, but the biochemical method is more accurate and reproducible. This biochemical technique allows us, for the first time, to place the exotoxin excretion during the sporulation stage and not during the exponential growth.

Bacillus thuringiensis↗
PubMed1975

[Interest of some additional biochemical tests for the classification of "Bacillus" (author's transl)].

In addition to previous taxonomic work, eight biochemical tests have been applied to the study of 580 Bacillus strains belonging to 22 species. The attack of chitin, melibiose and amygdalin can be used for the differentiation of some species. It is the same with most of the enzymes studied here, although Tween-esterase and DNase are often present in the majority of the isolates. RNase is still more frequent, being found in nearly all the strains. On the other hand, arginin-dihydrolase appears to be very rare and ornithin-decarboxylase seems always absent.

Amygdalin↗