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Biomedical subjects

H Zhuang

Publications and source records attributed to H Zhuang.

At least 127 records · Page 7Linked to original sources
PubMed1990

Effect of dietary cholesterol on the lipoprotein profile and binding of radioiodinated lipoproteins to hepatic membranes in the cockerel (Gallus domesticus).

1. Cockerels fed a cholesterol-supplemented diet experienced a marked elevation of lipoprotein particles of density less than or equal to 1.006 g/ml (VLDL) and a diminution of lipoprotein particles of density 1.02-1.05 g/ml (LDL). 2. Unlike VLDL of some cholesterol-fed animals, cholesterol-fed cockerel VLDL did not display beta-mobility on agarose gel electrophoresis. 3. [125I]LDL and [125I]HDL binding to cockerel liver membranes was not affected by cholesterol feeding. 4. Different lipoprotein types appear to bind to a common site on cockerel liver membranes. 5. The results suggest that liver cells of cockerels may not possess LDL binding sites that are analogous to those of mammalian species.

Animals↗
PubMed1990

Effect of radix Salviae miltiorrhizae extract injection on survival of allogeneic heart transplantation.

The effect of Radix Salviae Miltiorrhizae (RSM), a commonly used herbal blood circulation invigorator for the treatment of blood stasis in traditional Chinese medicine, on the duration of allograft survival following heterotopic heart transplantation in experimental animals was observed. The results in three heart transplantation models--auricular free graft in mice, abdominal graft in rats and cervical graft in rabbits--suggested that RSM injection in an appropriate dosage prolonged the survival time of cardiac allograft. The herb showed no significant toxicity. It was also found that RSM injection had a synergic effect with corticosteroids against graft rejection.

Animals↗
PubMed1990

[Effect of oxymatrine on prolonging the survival time of cardiac tissue allograft in mice and its immunologic mechanisms].

Oxymatrine is an extract from Sophora flavescens Ait. A daily dose of 75 mg/kg or 225 mg/kg of oxymatrine was given to the recipient intramuscularly for 14 days. The survival time of cardiac tissue allograft was prolonged significantly to 12.2 days (at the dose of 75 mg/kg, P less than 0.05) and 15.7 days (at the dose of 225 mg/kg, P less than 0.001) by oxymatrine, while that in the control group was 10.8 days. The effects of oxymatrine on immune-function in BABL/c mice with or without heart allograft were further studied. Experiments showed that in vitro spontaneous proliferation of spleen cells increased markedly on the 10th day after transplantation, while the proliferation response to Con A of spleen cells decreased. The spontaneous proliferation and proliferation responses to Con A or to LPS of spleen cells could be inhibited significantly in normal mice by oxymatrine. The proliferation response to LPS of spleen cells and RPFC was inhibited obviously in transplanted mice by oxymatrine. However, oxymatrine did not affect the proliferation response to Con A of spleen cells, which had been decreased after transplantation. The results suggested that this drug exhibited selective immuno-suppression on function of B cells without obvious effect on T cell function in transplanted mice. This characteristics of the drug seemed beneficial for avoiding side-effect produced by the conventional immuno-suppressive agents.

Alkaloids↗
PubMed1983

A cross sectional study of markers of hepatitis B infection in Niue.

To determine whether the island of Niue would be a suitable location to evaluate the efficacy of hepatitis B vaccine, the prevalence of hepatitis B infection in the adult population was studied. Sera were collected from 1147 of 1244 residents above the age of 20 years, and tested for the presence of hepatitis B surface antigen (HBsAg) and specific antibody to the surface and core antigens (anti-HBs and anti-HBc) by solid-phase radioimmunoassay (SPRIA). Hepatitis B was found to be hyperendemic; 11.9% of those tested were found to be carriers of HBsAg and an additional 84% had detectable levels of anti-HBs or anti-HBc indicative of current or past infection. In this population HBV infection appears to occur early in life as the peak prevalence of serological markers was found in young adults. The almost universal infection of the population, their high rate of compliance with the study and the relatively high birth rate indicate that Niue would be a suitable location to evaluate methods of preventing hepatitis B infection.

Adult↗
PubMed1983

Characterization of a precipitating antigen detected in the serum of patients with viral hepatitis.

During a search for the aetiological agent of non-A non-B hepatitis, a precipitating antigen was detected in the sera of some patients during the acute phase of their illness. The antigen was detected by agar gel diffusion using antibody from convalescent sera obtained from patients with non-A non-B hepatitis, and from haemophiliac sera. The antigen was usually detected early in the patient's illness, disappearing as liver function tests returned to normal. In some patients specific antibody appeared during the convalescent phase of the disease. The antigen does not appear to be specific for non-A non-B hepatitis, as it could be detected with similar frequency in patients with hepatitis A or hepatitis B and some patients with other liver disorders. Biochemical and biophysical studies suggest that the antigen is probably an abnormal lipoprotein produced as a result of acute liver damage.

Antigens↗
PubMed1983

Serum high density lipoprotein cholesterol and factors influencing its level in healthy Chinese.

Serum HDL-cholesterol (HDLc) levels were studied on 2399 healthy urban inhabitants, from newborn to 78 years of age, in Shanghai. The results were as follows. (1) Frequency distribution of serum HDLc levels in most groups was approximately normal after age 20. (2) The serum HDLc levels and HDLc/total cholesterol ratio varied with age and sex among healthy subjects. The mean values of HDLc for people over 30 years of age were 58.7 +/- 11.2 mg/100 ml for males and 62.5 +/- 12.2 mg/100 ml for females. The difference was of statistical significance (P less than 0.01). (3) The mean values of serum HDLc were found to be 40.9 +/- 6.0 mg/100 ml and 40.7 +/- 7.9 mg/100 in male and female neonates respectively. These figures are quite similar to those reported from Western countries and Japan. The serum HDLc level rose steadily with age and such elevation was more marked in females than males before age 30. However, the values became closer at age 60, then began to decrease in both sexes. (4) Serum HDLc levels varied markedly with body weight; they correlated negatively with the weight/height index. (5) No statistically significant relationship was found in Chinese between smoking or drinking and serum HDLc levels. The high level of HDLc seemed a result of environmental factors and is, possibly, related to the lower incidence of coronary heart disease in China.

Adolescent↗
PubMed1982

Seroepidemiology of infection with hepatitis B virus in Fiji.

A batch of 984 sera obtained from a stratified sample of Melanesians and Indians living in rural and urban areas of Fiji in 1981 were for hepatitis B surface antigen (HBsAg) and antibody to hepatitis B core antigen (anti-HBc) by solid phase radioimmunoassay. The prevalence of hepatitis B infection (as measured by the sum of HBsAg and anti-HBc frequencies of HBsAg negative sera in the two groups) was 81.5% and 17.9%, respectively. No major differences were detected between urban and rural populations. While hepatitis B virus is endemic in Melanesians and Indians, the epidemiology of the infection shows certain differences. Among Melanesians, infection appears to be acquired early in life and peak prevalence of serologic markers of infection occurs during the second decade. Among the Indian population, the prevalence of markers increases steadily with age, presumably as a result of continuous exposure and infection throughout life. the high prevalence of infection and carriers among Melanesians is consistent with previous observations among Pacific populations. The lower prevalence of infection among Indians is remarkable, since they constitute almost half of the total population and live under similar conditions. Since the two populations remain largely separate in terms of housing and schooling, and intermarriage is uncommon, it is no possible to determine whether these differences merely represent different degrees of exposure to the virus or are the reflection of differences in susceptibility or response to infection.

Adult↗
PubMed1982

Serum immunoglobulin levels in acute A, B, and non-A, non-B hepatitis.

Immunoglobulin M, G, and A concentrations were determined by radial immunodiffusion in sera collected from 117 patients with acute hepatitis A, hepatitis B, or non-A, non-B hepatitis. Statistically significant differences in IgG and IgM levels were detected between the three groups. In particular, elevated IgG and almost-normal IgM levels were regularly detected in patients with non-A, non-B hepatitis while the opposite pattern was seen in patients with hepatitis A. Calculation of a serum IgG/IgM ratio enabled discrimination between most patients with non-B hepatitis. In 92% of patients with hepatitis A, the ratio was less than 6, whereas 82% of patients with non-A, non-B hepatitis had ratios of greater than 6. The IgG/IgM ratio may be of value in distinguishing between subjects with the two forms of the disease when specific serologic tests are unavailable.

Hepatitis C↗
PubMed1982

Detection of markers of hepatitis B infection in serum dried on to filter-paper: an application to field studies.

In an attempt to find a cheap, reliable, and convenient method for the transportation and storage of serum specimens during seroepidemiological surveys, a technique in which serum was dried on to pieces of filter paper was developed and evaluated. For the evaluation, a total of 382 sera were selected from the extensive serum collection held by the WHO Collaborating Centre for Virus Reference and Research at Fairfield Hospital, Australia. These sera were dried on to pieces of filter-paper, stored at different temperatures and then tested for the presence of the various markers of infection with hepatitis B virus by solid-phase radioimmunoassay. The results were in complete agreement with those obtained on whole serum specimens. In addition, storage at 4 degrees C, room temperature (22 degrees C), or 37 degrees C for up to 30 days did not alter the sensitivity of the test. This technique may be useful in field surveys, not only for the detection of hepatitis B infection, but also in the study of other diseases and metabolic disorders.

Australia↗
PubMedDate not supplied

Assessment of body fat and lean in the elderly by measuring body carbon and oxygen: validation against hydrodensitometry.

Based on the observation that the carbon-to-oxygen ratio (C/O) in tissue is a measure of fat content, we developed a model which correlates C/O to percent body fat. Carbon and oxygen mass and their ratio are measured in vivo by fast neutron inelastic scattering, using a miniature D-T neutron generator, at a radiation exposure of less than 0.06 mSv. We tested the validity of this model against hydrodensitometry with 19 healthy adult volunteers. The method was found to be accurate and insensitive to assumptions about the composition of lean tissue and, therefore, appropriate for studying the elderly and patients with catabolic conditions.

Adipose Tissue↗
PubMedDate not supplied

Use of fast neutrons for measuring muscle.

Elemental Partition Analysis (EPA) is a new approach to body composition. A major element of the body is measured and then, by means of other measurements, is partitioned to the contributing body compartments. We developed a model for measuring total body muscle by applying the EPA method to total body phosphorus (TBP). We measure TBP (head excluded) by in vivo fast neutron activation analysis using the reaction 31P(n, alpha)28 Al. The main contributors to TBP are the bone and skeletal muscle. Adipose tissue and the liver contribute less than 3%. We use dual-energy X-ray absorptiometry (DXA) to evaluate the contribution of bone to TBP. Corrections are applied for the small contributions of the liver and adiopose tissue to TBP to derive muscle phosphorous. The technique requires high precision measurements for both TBP (+/- 2%) and DXA (+/- 1%). The total body radiation exposure for measuring human subjects is 0.60 mSv. We observed that nitrogen can also be measured by fast neutron activation analysis through the (n,2n) reaction, simultaneously with TBP.

Adipose Tissue↗
PubMedDate not supplied

Detection of mutA genes in transmitted strains and nontransmitted strains of mutans streptococci.

Our aim was to determine whether an isolate carrying one of the mutA genes was related to its ability to be transmitted from mother to her child. First, 200 mutans streptococci isolates were genotyped by arbitrarily primed polymerase chain reaction (AP-PCR) to demonstrate transmission between 20 mother-child pairs and to detect the transmitted and nontransmitted strains. Then the mutacin structural genes mutA encoding mutacin types I, II, and III were screened by PCR. The results showed that all strains found to carry the mutAI gene were nontransmitted strains; PCR screening primers mutAII and mutAIII did not yield amplicons in any of the strains tested. Our data suggest that an isolate carrying the mutAI gene is related to reduced transmission. The low frequency of detection of mutAII, and mutAIII suggests that there is a high heterogeneity in the genetic determinants needed for the production of mutacin-like substances.

Adult↗
PubMedDate not supplied

Positron emission tomography imaging in evaluation of cancer patients.

Positron emission tomography (PET) is a diagnostic imaging technique that has progressed rapidly from being a research technique in laboratories to a routine clinical imaging modality. The most widely used radiotracer in PET is Fluorine18-fluorodeoxyglucose (F18-FDG), which is an analogue of glucose. The FDG uptake in cells is directly proportional to glucose metabolism of cells. Since glucose metabolism is increased many fold in malignant tumors PET has a high sensitivity and a high negative predictive value. PET with FDG is now the standard of care in initial staging, monitoring the response to the therapy, and management of lung cancer, colonic cancer, lymphoma, melanoma, esophageal cancer, head and neck cancer and breast cancer. Other indications of PET like bone tumor, ovarian cancer and cancer of unknown primary (CUP) has also been discussed in brief. The aim of this review article is to review the clinical applications of PET in various malignancies and only limited number of important studies will be discussed for this effort.

Fluorodeoxyglucose F18↗