Transitions from conventional metals and insulators to Kondo metals and insulators.
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Journal Article↗
Biomedical subjects
H Zheng
Publications and source records attributed to H Zheng.
Effect of quantum-lattice fluctuations in one-dimensional fluctuating-valence systems.
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Journal Article↗
Variational treatment of retarded phonon-induced electron pairing.
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Journal Article↗
A novel granulocyte-derived peptide with lipopolysaccharide-neutralizing activity.
Rabbit CAP18 (cationic antimicrobial protein, 18 kDa) is a leukocyte protein identified and purified using as an assay its capacity to bind and inhibit various activities of LPS. Oligonucleotide probes designed from the putative N-terminal protein sequence were used to obtain the corresponding cDNA from a rabbit bone marrow cDNA library. Examination of the cDNA sequence revealed that the protein fragment of the putative N-terminus was actually a 37-amino-acid C-terminal fragment. This fragment, designated CAP18(106-142), inhibits many activities of LPS. In the present studies, synthetic CAP18(106-142) is shown to: 1) bind to erythrocytes coated with diverse strains of LPS; 2) inhibit LPS-induced release of cytokines (TNF, IL-1, IL-6) and nitric oxide from macrophages; 3) inhibit LPS-induced LAL coagulation and 4) protect mice from LPS lethality. CAP18(106-142) may have therapeutic utility for conditions associated with elevated concentrations of LPS.
Amino Acid Sequence↗
Exclusion of ferritins and iron-responsive element (IRE)-binding proteins as candidates for the hemochromatosis gene.
We have looked for genes for ferritin and its translational control protein that could account for anomalies in the expression of ferritin (FT) and the transferrin receptor in the duodenum of individuals with hemochromatosis (HC). We show that there are probably only two FTH-like sequences near the HC locus on the short arm of chromosome 6 and no FTL-like sequences. We report the cloning of the previously uncharacterized FTH sequence from 6p (FTHL15) and show that it is probably a processed pseudogene. This gene has been mapped with a panel of radiation hybrid cells to near 6p12. Additionally, we show that there are no sequences on chromosome 6p for a protein that coordinately regulates expression of ferritin and the transferrin receptor.
Animals↗
Primary culture of rat gastric epithelial cells as an in vitro model to evaluate antiulcer agents.
Primary rat gastric cell cultures were investigated as an in vitro model for evaluating antiulcer agents. Following exposure to concentrations of up to 5 mg/mL of an antiulcer agent sucralfate, an aluminum hydroxide complex of sucrose octasulfate, cultured cells were treated with either pH 3.5 medium or 3.5 mM indomethacin. Cytoprotection was evaluated by colony forming efficiency, neutral red uptake, and 3-(4,5-dimethyl-2-thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) hydrolysis. By each measure, and depending on damaging agent, 2 and 5 mg/mL sucralfate provided partial (50% of untreated control) to near-complete (90% of untreated control) cytoprotection, respectively. Aluminum hydroxide also provided partial (55% of untreated control) to near-complete (more than 90% of untreated control) cytoprotection at 2 and 5 mg/mL, respectively, for the pH 3.5 medium-induced damage. Over a concentration range of 0.05 to 5 mg/mL, the potassium salt of sucrose octasulfate, KSOS, stimulated cell growth up to 40-60% over untreated controls but had little or no cytoprotective action in the presence of either 3.5 mM indomethacin or pH 3.5 medium. Overall results suggested that sucralfate may have at least two roles in influencing gastric epithelial cell function, cytoprotection and stimulation of cell growth in vitro. These observations serve as a basis for further study of in vitro models in evaluating the cytoprotective activity of antiulcer agents and their respective mechanisms of action.
Acids↗
Evaluation of a recombinant parasite antigen for the diagnosis of lymphatic filariasis.
We evaluated the usefulness of a recombinant parasite antigen (recSXP1) for the serologic diagnosis of lymphatic filariasis. A large proportion of sera from microfilaremic donors living in five different endemic countries (356 of 446 [80%]) contained IgG antibodies to recSXP1, as do sera from approximately 33% of amicrofilaremic patients with acute filarial disease and/or indirect evidence of active filarial infection. Exposure to filarial worms per se does not appear sufficient to elicit an anti-SXP1 antibody response. Thus, this serologic test identifies a large proportion of persons with active lymphatic filariasis among residents of endemic areas.
Animals↗
[Customs, habits and medical culture in the Qin-Han Periods].
The unity of Qin-Han feudal dynasties improves the development of society, flourishes the economy and creates a good environment for friendly communication and fusion of customs among all nationalities, exerting positive and active influence on medicine. Analyses are made on the relation between customs and habits, and medical culture. it is stressed that not all customs are in line with science. Some of them might even be superstitious or anti-scientific. However, in some sense, it is the soil for the advent of medical and health care issues.
China↗
[Experimental study on the reinnervation of the vocal cord adductor by implantation of ansa cervicalis].
The sub-branches of main branch of ansa cervicalis were cut and implanted into the thyroarytenoid and lateral cricoarytenoid muscles on the left side in 7 dogs after the recurrent and superior laryngeal nerves were sectioned on the same side. After six months, laryngoscopy showed that adduction of the vocal cord recovered in five post-operative cases. Adduction was caused by the reinnervated adductor muscles, as demonstrated by laryngeal electromyography, histologic studies, tension of contraction and electron microscopy for the neuro-muscular junctions. The present results demonstrated that the procedure seemed to produce satisfactory reinnervation of the adductor musculature.
Animals↗
[Experimental study on delayed reinnervation of vocal cord adductors].
The adductor branch was anastomosed with the main branch of ansa cervicalis to reinnervate the vocal cord adductors in six dogs at the time of denervation and after varying intervals of chronic denervation. Some recovery of adduction was noted within ten-month interval. Laryngeal electromyography, tension tests and histologic studies of the adductor muscles were correlated with the recovery of adduction. Although adduction didn't recover after twelve-month interval, striations of the adductor muscles were normal, only slight fibrosis was observed.
Anastomosis, Surgical↗
Rabbit CAP18 derived peptides inhibit gram negative and gram positive bacteria.
CAP18 (cationic antimicrobial protein of 18 kDa) was originally isolated from rabbit granulocytes using as an assay the agglutination of Re-lipopolysaccharide coated erythrocytes. The C-terminal 37 amino acids of CAP18 comprise the LPS-binding domain called RNIP, reactive nitrogen inhibitory peptide. Synthetic RNIP has broad antimicrobial activity versus both gram positive [IC50 = 130-200 nM] and gram negative bacteria [IC50 = 20-100 nM). Susceptible strains include Staphylococcus aureus, Klebsiella pneumoniae, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes. Antimicrobial activity is highly dependent upon peptide structure. Although a 32 amino peptide resulting from truncation of five amino acids from the C terminus of RNIP is highly active, other fragments of RNIP including truncation of its N-terminus do not exhibit antimicrobial activity. Unlike previously characterized antimicrobial peptides derived from granulocyte proteins RNIP is active in serum. RNIP or a derivative peptide may have therapeutic potential for bacterial sepsis.
Amino Acid Sequence↗
[A prospective study on the relationship between body mass index and mortality from all causes in 15,827 middle aged and elderly citizens].
The association between Body Mass Index (BMI) and mortality from all causes was investigated in a cohort study of 15827 citizens (aged 35 and over) in Shanghai. During about 3-year follow up the person-year mortality for men and women were 1311.78/100000 and 1036.87/100000, respectively. Cox regression analysis was conducted in a lower and an upper range of BMI, respectively. The inverse association between BMI and mortality in the lower range was statistically significant for men and women. The positive association between BMI and mortality in the upper range was highly statistically significant for women but not for men. Logistic regression analysis conducted over the entire range of BMI showed that BMI and all-cause mortality association was significantly "L"-shaped distribution for men and women. The mortality or relative hazard curve can be fit with quadratic model (Y = a-bX + cX2) in different sex.
Aged↗
[Intravenous placental immunoglobulin for treatment of chronic graft versus host disease].
Intravenous placental immunoglobulin (IV Pt IG) was used for the treatment of chronic graft versus host disease (CGVHD) in 30 patients who are refractory to steroid and cyclosporinic A. 15 (50%) patients showed excellent response and 11 (36.66%) good response. The total response rate is 86.66%. The dosage of IV Pt IG was 4 gm/day in adults by intravenous infusion. Effectiveness of IV PtIG was discerned within 2 weeks. The plasma levels of IgG, IgA and IgM were tested before and after IV PtIG treatment. There was no significant statistical difference between the plasma IgG, IgA, IgM levels in pre- and post-treatment period. The efficacy of IV PtIG against CGVHD is therefore ascribed to its pharmacological effect.
Adolescent↗
[Clinicopathological analysis of rhabdomyosarcoma of the ocular adenexa].
12 cases including 11 males and 1 female of rhabdomyosarcoma of ocular adenexa were analysed. The average age of the patients was 4.2 years. 9 cases of lesion were located in the orbit, 2 in the eyelid and 1 in conjunctiva. Pathologically 8 cases were embryonal forms, 2 alveolar, 2 polymorphic. Immunohistochemical studies were performed in 8 cases, which showed desmin positive. Myoglobin presented strong or weak positive in 5 cases, suspicious positive in 3 cases. The clinical manifestation, prognosis and treatment were briefly discussed.
Child↗
[Study on genetic epidemiology of albinism].
Segregation analysis and consanguinity analysis were applied to 37 albinism families ascertained from Shandong province (P. R. China) to study the genetic heterogeneity of the disease. Our results show that albinism is an autosomal recessive disease with multilocus. The least number of the detrimental loci is 8, The average frequence of detrimental genes is 0.0023, and 3.83% individuals in general population are carriers. The incidence among the offsprings of the first cousin marriages would be 22 times higher than that in the general population.
Albinism↗
[A community cohort study on risk factors of strokes in Shanghai: a Cox regression analysis on 15,885 subjects].
Baseline investigation on 15,885 subjects who were over thirty-five years old had been launched since July 1987. All of them were selected from eighteen neighbourhoods of three districts in Shanghai by using multiple stage cluster sampling procedure. All of them were followed up till the end of 1989. Each new case of stroke was randomly matched with about twenty controls by age and sex. The total 2,682 controls together with 127 new cases of strokes were analyzed by using Cox regression model for risk factors. The population attributable risk proportion (PARP) of factors was also estimated.
Acute Disease↗
[Application of synthetic 27 oligopeptide of HDV antigen for detecting serum anti-HD in HBV infected subjects in Chongqing].
A synthetic HDAg 27 peptide which was selected and designed by the authors and synthesised by Shanghai Institute of Biochemistry, Chinese Academy of Science was used with ELISA method to detect serum anti HD in HBV infected subjects in Chongqing. Anti HD was positive in one of 300 blood donors and was negative in all of 113 cases of hepatitis A and 58 cases of hepatitis non-B. Anti HD was positive in 106 out of 882 cases with positive HBV marker (12.02%), among which anti HD was positive in 3.17% (13/410) of HBsAg carrier, 14.4% (11/76) of acute hepatitis, 7.6% (1/13) of chronic persistent hepatitis, 17.68% (22/121) of chronic active hepatitis, 19.77% (17/86) of severe hepatitis, 29.49% (23/78) of liver cancer and 19.39% (19/98) of primary hepatic cancer. These results coincided with those of previous reports. The coincidence rate was 94.9% (74/78) when compared with Abbott EIA kit. When the natural HDAg was used to compete anti HD in four anti HD positive and two anti HD negative serum specimens, anti HD was negative in all specimens. It is shown that the HDAg 27 peptide has natural HDAg activity capable of being recognized by natural anti HD and is a new diagnostic agent being more simple, save, stable and reliable.
Antibodies, Viral↗
[Cloning and DNA sequencing of the gene encoding trichosanthin].
A gene encoding Trichosanthin (TCS) was amplified by means of DNA polymerase chain reaction (PCR) and cloned from the genomic DNA of Trichosanthes kirilowii. Its DNA sequence has been determined. Nucleotide sequence analysis indicates that the cloned TCS gene includes 5'-flanking region and the encoding sequence for TCS preproprotein. Comparison of its encoding sequence with the published nucleotide sequences of three TCS genes demonstrates that they have the sequence homologies of 99.20%, 98.74% and 98.64% respectively. Comparing its deduced amino acid sequence with four reported amino acid sequences of TCS demonstrates that they have the sequence homologies of 98.62%, 97.62%, 97.41% and 98.38% respectively. A new TATA-like box sequence was found in the 5'-flanking region of cloned TCS gene. In order to further study the structure, expression, regulating mechanism of TCS gene and the structure-functional relationship of TCS, a series of mutants of TCS gene has been constructed. The expression of these mutants in bacteria and transgenic plant has being studied.
Amino Acid Sequence↗