Biomedical subjects

H Zheng

Publications and source records attributed to H Zheng.

At least 235 records · Page 13Linked to original sources

PubMed1998

Preparation of highly purified hemoglobin by affinity elution.

The large scale production of recombinant hemoglobin (Hb) from microorganism or transgenic hosts for Hb-based blood substitutes places utmost emphasis on purity. In the present study, a high-resolution, convenient and inexpensive purification method is developed for purification of Hb from mixtures containing E. coli extract and bovine serum. This method is based on affinity elution by pyrophosphate (PPi) of Hb adsorbed on an FPLC column of the anion exchanger Toyopearl DEAE-650M. Compared to pH elution or NaCl elution, PPi elution makes possible the preparation of Hb of much higher purity. A procedure combining pH elution and PPi elution sequentially using a single column proves particularly valuable. The purification method is also applicable to the purification of cyanomet-Hb (CNHb+).

Chromatography, Affinity↗

PubMed1998

[An epidemiology study on common diseases among the elderly in Beijing].

In order to study the status and characteristics of some common diseases of elderly which seriously influence the Quality of Life of the elderly, 1434 elderly over 60 years old in the urban and rural areas of Beijing were investigated with a random sampling method. The results showed that prostate hyperplasia, deafness, cataract, osteoarthropathy, bone fracture and constipation among the elderly were 61.4%, 53.9%, 46.4%, 24.4%, 14.2% and 18.2% in the urban areas, and 65.7%, 64.7%, 44.4%, 14.9%, 9.1% and 23.0% in the rural areas respectively. The prevalence rates of osteoarthropathy (P < 0.01) and bone fracture (P < 0.01) were higher in the urban areas than in the rural areas (P < 0.01). In contrast, the prevalence rates of deafness (P < 0.01) and constipation (P < 0.05) of the elderly were higher in the rural than those in the urban. The prevalence rates of above diseases were lower when selfreporting than that by the medical examination, and also lower in the rural than in the urban (P < 0.01). Hence, the prevention and treatment of the common diseases of elderly should be strengthened, especially in the rural areas.

Aged↗

PubMed1998

Study on acupuncture and moxibustion therapy for female urethral syndrome.

Among 180 patients with female urethral syndrome, 128 were treated by acupuncture and moxibustion and 52 by western medicine as controls. The short-term effective rate in the acupuncture and moxibustion group was 90.6% and the long-term effective rate, 80.4%; whereas the short-term effective rate of the control group was 26.9% (P < 0.01). The maximal uroflow rate increased by an average of 4.6 ml/s, after acupuncture and moxibustion treatment (P < 0.001) and the mean uroflow rate increased by an average of 3.1 ml/s (P < 0.001); on the contrary, no changes were found in the control group (P > 0.05). Sixty-nine cases from the acupuncture and moxibustion group and 39 from the control group were subjected before and after treatment to determinations of the maximal bladder pressure, maximal abdominal pressure, bladder-neck pressure, and maximal urethral closure pressure during urination. All these indexes were decreased remarkably in the acupuncture and moxibustion group, while no changes were observed in the control group.

Acupuncture Therapy↗

PubMed1998

[Genotype distribution of hepatitis C virus in hepatocellular carcinoma tissue in Hunan province].

Genotypes of hepatitis C virus(HCV) were detected by PCR using type-specific primer in 50 patients' hepatocellular carcinoma(HCC) tissue. The results showed that in the 50 HCC specimens, 30(60%) and 3(6%) were infected with the HCV type II and III, 5(10%) and 8(16%) with type II + III and type II + IV, 2(4%) with type II + I + III in combination, respectively. 2 cases were negative for HCV. These data suggest that HCV type II may be a predominant genotype related to hepatocarcinogenesis in Hunan Province, some cases of HCC may result from coinfection of HCV type II and other genotypes, and only few HCC be separately caused by infection of HCV type III.

Adult↗

PubMed1998

[Study on relationship between apoptosis and proliferation of cells in liver cirrhosis and hepatocellular carcinoma].

Apoptosis and nuclear antigen of proliferating cells were detected by labelling technique of in situ terminal deoxynucleotide transferase and immunohistochemical method in liver cirrhosis and hepatocellular carcinoma(HCC). The density of apoptotic cells in HCC was significantly lower than that in cirrhosis, and the density of proliferating cells was much higher in HCC than that in cirrhosis. Apoptotic cells mainly distributed in the peripseudolobular region of cirrhosis and formed an apoptosis zone. But they scattered within the cancer tissue. The results suggest that the formation of apoptosis zone in cirrhosis may be related to the change of liver blood stream. Selective proliferation of cells may exist during carcinogenesis of liver cirrhosis.

Adult↗

PubMed1998

[The factors affecting apoptosis by in situ terminal deoxynucleotide transferase method].

Apoptosis in biopsies of patients with hepatocirrhosis or hepatocellular carcinoma and in small intestinal mucosa of mice were detected by the method of in situ terminal deoxynucleotide transferase (ISTdT). It was found that the best positive result might be obtained by digesting tissue sections with 25mg x L-1 proteinase K for 15 minutes, then treating sections with moderate degree of microwave for 5 minutes after reaction with terminal deoxynucleotide transferase. The best choice of above conditions were discussed.

Animals↗

PubMed1998

[Comparative study of tetracycline-estrone and estrone effects on bone histomorphometric parameters in ovariectomized rats].

OBJECTIVE: To compare the effects of tetracycline-estrone (TE) and estrone (E) on bone histomorphometric parameters of femoral distal diaphysis in ovariectomized (OVX) rats. METHODS: Twenty female rats were randomly allocated into four groups, 5 rats in each: tetracycline-estrone (TE), estrone (E), OVX and sham operation (S). The OVX rats were used as a model for osteoporosis. After being fed with TE or E for 13 weeks, all rats were sacrificed. The effects of TE and E on bone microarchitecture and dynamics were studied by bone histomorphometry. RESULTS: The histomorphometric data showed that the connectivity of trabecular bone in TE and E groups was significantly improved in comparision with that in S group (P < 0.05). The dynamic data indicated that in TE and E group, the tetracycline labelled and osteoid surfaces were remarkably increased in comparision with those in OVX group, especially the data in TE group were significantly higher than those in E group and other two groups (P < 0.05). CONCLUSIONS: The connectivity of trabecular bone could be significantly improved by both TE and E. The activation frequency seemed to be higher in TE group than that in E group.

Animals↗

PubMed1998

[Effects of the new compound-XW630 on osteoblast].

This study aimed to observe the influence of the new compound-XW630 on the proliferation of osteoblast, ALP action, and the forming of osteoclast. The MTT method and the alkaline phosphatase method were adopted to investigate the influence of XW630 on ALP action and the proliferation of osteoblast cultured from grown rat's skull. The Tartrate-resistant acid phosphatase dyeing method was used to observe the influence of XW630 on forming of cultured osteoclast in vitro. The result showed that, when XW630 concentration was 10(-6)-10(-8) mol/L, it obviously enhanced the proliferation of osteoblast and improved the activity of ALP, and it evidently provented PTH from stimulating the forming of osteoclast. It is concluded that XW630 is obviously effective for stimulating the proliferation of cultivated osteoblast in vitro and for inhibiting the forming of osteoclast.

Alkaline Phosphatase↗

PubMed1998

[The effect of HCV NS3 protein on expression of p53 gene protein in hepatocarcinogenesis].

OBJECTIVE: To investigate hepatocarcinogenesis by detecting the effect of HCV NS3 protein on expression of P53 protein in hepatocellular carcinoma (HCC) and pericarcinomatous liver tissue. METHODS: The expression of HCV NS3 and P53 protein was detected by immuno-histochemical technique (SP method) in specimens of HCC and their surrounding liver tissues from 47 patients with negative HBV. RESULTS: The positive rate of HCV NS3 protein was lower in HCC(62%) than in pericarcinomatous liver tissue(83%) (P < 0.025). The expressive strength of HCV NS3 protein in HCC was related to the degree of carcinomatous cell differentiation(P < 0.025). The positive rate of P53 protein in carcinomatous tissue(81%) was higher than in pericarcinomatous liver tissue (47%)(P < 0.025). The worse differentiation of cancer cells, the stronger expression of P53 protein (P < 0.05). The expression of P53 protein was not correlated with the expression of HCV NS3 protein in carcinomatous tissiue (P > 0.5), whereas their expression was closely related in pericarcinomatous liver tissue(P < 0.01), and the expressive rate of P53 protein in the cases of positive HCV NS3 protein was higher than that in the cases of negative HCV NS3 protein. CONCLUSION: HCV NS3 protein may exert its hepatocarcinogenic effect in early stage on host cells by endogenous pathway which may bring about mutation of p53 gene and transformation of hepatocytes.

Carcinoma, Hepatocellular↗

PubMed1998

[Treatment of malignant hematological diseases with autologous peripheral blood stem cell transplantation].

OBJECTIVE: To observe the effectiveness of autologous peripheral blood stem cell transplantation(ABSCT) in the treatment of malignant hematologic diseases. METHODS: Thirteen ABSCT and 15 autologous peripheral blood stem cells(ABSC) combining autologous bone marrow transplantation (ABMT) were performed for hematological malignancy patients. RESULTS: In polyactin A mobilization group, the mononuclear cells(MNC) collected in each case were 3.58 +/- 2.12 x 10(8)/kg, and in G-CSF mobilization group 6.68 +/- 5.31 x 10(8)/kg. Twenty-one of the 28 transplanted cases achieved continuous complete remission(CCR). The medium CCR duration was 18(5-58) month. The 3 year disease-free survival rate was 68.7% and relapse rate was 22.3%. CONCLUSION: After ABSCT or ABSC combining ABMT, there were earlier hematopoietic reconstitution and fewer complications than that after ABMT.

Adolescent↗

PubMed1998

Effects of combined radiation and thermal burn injury on the survival of skin allograft and immune function in rats.

OBJECTIVES: To investigate the effects of combined radiation and thermal burn injury on the survival of skin allografts and to analyze the relationship between the prolongation of allograft survival and the changes of immune functions of the thymocytes and splenocytes in rats. METHODS: Wistar rats were irradiated with 3, 4, 5, 6 and 8 Gy of gamma rays. Thirty minutes after radiation, 15% TBSA III-degree burn was inflicted to the rats. Twenty-four hours after the burn injury, allografts were used to cover the burn wounds. In the 8 Gy group, 1 hour before skin grafting, the bone marrow cells (4 x 10(8)) from the same donor were also transplanted. All rats were carefully observed after injury. The rats with single radiation injury of 5 Gy gamma rays, with single burn injury and with combined radiation-burn injury were killed 3, 7, 10, 15 and 30 days after skin grafting for immunological assay and pathological study. RESULTS: All the allografts in the single burn group were rejected in 10 days. In the combined injury groups, the survival rates of the allografts in rats undergoing 3 and 4 Gy radiation were 20% and 30%, respectively. In the combined injury groups undergoing 5, 6 and 8 Gy radiation, the 10-day survival rates of the allografts were 69%, 88% and 100% respectively, and the 30-day survival rates in the three groups were 36%, 42% and 100% separately. The grafted allogenic skin, with normal epithelial cells and good vascularity, healed well with the recipient's skin. Hairs grew well from the allografts 30 days after grafting. Three, 7 and 15 days after allografting, in the single burn group, the proliferative activities of the thymocytes were 90%, 185% and 130% of the preinjury level, and the antibody forming capacities of the splenocytes were 200%, 171% and 300% of the preinjury level, respectively; in the combined injury groups, the proliferative activities were 6%, 99% and 91% of the preinjury level, and the forming capacities were 2%, 36% and 90% of the preinjury level. CONCLUSIONS: The survival rate of allograft in rats undergoing combined radiation and thermal burn injury rises with the increase in radiation dosage. The allograft covering single bun injury is severely rejected by immune reaction. The prolongation of the survival of allograft in combined injury group mainly results from radiation that suppresses immune functions.

Animals↗

PubMed1998

[Interleukin-6 gene cloning, expression and purification].

OBJECTIVE: The main purpose of this paper is to study the batch production of recombinant human interleukin-6(rhIL-6). METHODS: The cloned rhIL-6 gene is under the control of T7 promoter of pET30a vector and expressed in E. coli. RESULTS: The ratio of expressed recombinant protein to total cell protein is more than 50%. The rhIL-6 molecular weight is 21,000, isoelectric point is 6.7. The purity of the rhIL-6 is more than 95%, and the activity of rhIL-6, determined by IL-6 dependent mice hybridoma cell line 7TD1 and MTT assay, is 0.35 ng/ml. CONCLUSIONS: All results mentioned show that rhIL-6 meets the request of the middle scale production.

Cloning, Molecular↗

PubMed1998

[Identification and study on effect of new stress protein from Neisseria gonorrhoeae].

OBJECTIVE: To investigate the influence of changing environmental growth conditions on the virulence of Neisseria gonorrhoeae. METHODS: The gonococci were subjected to 1 mmol/L hydrogen peroxide (sublethol concentration) for one hour. RESULTS: A novel gonococcal stress protein with molecular weight of 62,000 was identified by radioactive labeling and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Gonococci exposed to 1.0 mmol/L hydrogen peroxide made it significantly more resistant to higher concentrations of hydrogen peroxide and to neutrophils than control organisms (P < 0.01). CONCLUSIONS: These results suggest that the 62,000 stress protein might increase the virulence of N. gonorrhoeae and might be an adapted regular protein produced by N. gonorrhoeae survived within human neutrophils.

Chaperonin 60↗

PubMed1998

[Determination of oil contents and fatty acids in seeds of Torreya Arn. in China].

OBJECTIVE: To analyze the oil contents and fatty acid composition in the seeds of Torrreya in China. METHOD: The contents and composition were determined by gravimetric analysis and GC respectively. RESULT: The oil contents in the seeds were 42.67%-54.39%. Among the fatty acids, linoleic acid and oleic acid were dominant. The unsaturated fatty acids accounted for 76.1%-82.0% of the whole fatty acids, and the major saturated fatty acids were behenic acid and palmitic acid. CONCLUSION: The oil in the seeds of Torreya is of high-quality, and the higher contents of bdhenic acid are characteristic for the oil. The development and utilization values of the seed oil in Torreya are evaluated.

Cycadopsida↗

PubMed1998

[The estrogenic activities of 2-[3-estrone-N-ethyl-piperazine-methyl]tetracycline(XW630)--a new compound with anti-osteoporosis activity].

2-[3-Estrone-N-ethyl-piperazine-methyl] tetracycline (XW630) showing anti-osteoporosis activity is a new compound with piperazine-tetracycline in the steroid structure of estrone. The affinity of XW630 with uterine estrogen receptor and its estrogenic activity were studied in this paper. The relative affinities of estrone, estradiol and XW630 with estrogen receptor (ER) of the uterine myoma tissues were found to be 0.325, 1.000 and 0.011, respectively, by the competitive assay. The estrogenic activity index of estrone, estradiol and XW630 were shown to be 9.70, 10.30 and 6.90, respectively, by the uterine weight assay using immature mice. These indexes indicate that: the estrogenic activity of XW630 is about 1/1960 that of estrone and 1/7742 that of estradiol. The vaginal smear assay of oophorectomized mice also showed that the estrogenic activity of XW630 is less than that of estrone and estradiol.

Animals↗

PubMed1998

[Synthesis and antiosteoporosis activity of steroid-bisphosphonate compounds].

Bisphosphonate have bone-seeking affinity, some of them are used as drugs for osteoporosis. Steroidal hormones are often used in prevention and treatment of osteoporosis. In order to search for bone-targeted leading compounds with high affinity and low toxicity, seven steroid-bisphosphonate compounds were synthesized. These compounds were found to promote bone formation in bone cell culture.

Animals↗

PubMed1998

[Application of immunochromatographic test for diagnosis and surveillance of bancroftian filariasis].

AIM: To evaluate the usefulness of immunochromatographic test (ICT) for rapid diagnosis and surveillance of Wuchereria bancrofti infection. METHODS: The monoclonal antibody based-ICT assay was used to detect filarial antigens in the sera from bancroftian filariasis patients. RESULTS: 111 out of 116 bancroftian microfilaria cases showed positive ICT reaction, giving a positive rate of 95.7%. Serum samples from 12 malayian microfilaremia cases, 33 ascariasis cases, 20 schistosomiasis japonica cases and 6 trichinellosis cases were all found negative in ICT, demonstrating a specificity of 100%. In 73 chronic filariasis cases with elephantiasis, hydrocoele, and chyluria cases, 18 showed ICT positive, among whom 16 revealed microfilaremia, suggesting that the ICT positive cases were with active infections. Of the 30 microfilaremia cases before single dose ivermectin treatment, 29 were ICT positive (96.7%). A negative conversion of microfilaremia was observed in all cases 8-14 days post-treatment. Upon following up to 6 and 12 months, microfilaremia reappeared in 5 and 7 cases, respectively. 5 out of 13 ICT positive cases were found microfilaremic at 6 month post-treatment, while among the rest of 8, 5 were found microfilaremic at 12 month post-treatment. Parallel use of ICT with routine blood film examination in Houda village, Zhecheng County, Henan Province for a post-control surveillance survey in 132 local individuals revealed 9 microfilaremia cases were also ICT positive. Of the 123 persons with negative blood examination, only 1 was ICT positive. CONCLUSION: ICT is a rapid and simple method with high sensitivity and specificity, and might be used for the diagnosis, efficacy evaluation as well as a tool for post-control surveillance of bancroftian filarasis.

Animals↗

PubMed1998

[Bio-mechanical property changes of long bone in ovariectomized goats].

This paper presents the biomechanical property changes of long bone in goats at 180 days after ovariectomy. 10 female goats at the age of 1.5 +/- years(average body wight 22 kg) were randomly divided into the control group and ovariectomized group. These goats were sacrificed at 180 days after ovariectomy and the femora, tibiae, metatarsus, humeri, radius and metacarpus were taken out bilaterally. The structural mechanical properties of these bones were measured with the three-point bending method. The material mechanical properties of flexura, compress and tensile in the compact bone specimens taken from the two broken ends were also measured. The results showed that the bone density, bone thickness in broken ends, and the break load of three-point bending for the long bones in the OVX group were obviously lower than those in the control group (P < 0.05). And compared with the control group all the long bones in the OVX group, except the femur, had significantly lower ultimate bending strength, bending elastic modulus; ultimate flexural strength, flexural elastic modulus; ultimate compressing strength, and compressing elastic moduus. Some long bones in the OVX group had significantly decreased ultimate tensile strength (P < 0.05). These indicated that the mechanical properties of long bone in OVX group at 180 days after ovariectomy were reduced in varying degrees and were liable to fracture. This result is in accord with the clinical findings in osteoporosis resulting from post-menopausal estrogen fall and is also consistent with the osteoporotic model of ovariectomized rats. The authors conclude that the bone biomechanical properties are important parameters in assessing osteoporosis and the ovariectomized goat can possible be a good big animal model of osteoporosis.

Animals↗