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Biomedical subjects

H Zankl

Publications and source records attributed to H Zankl.

At least 73 records · Page 4Linked to original sources
PubMed1976

Isonucleolinosis in cell cultures of human meningiomas.

In cell cultures of 13 human meningiomas the internal structure of the nucleolus was stained by the toluidine blue-molybdate method and compared with the karyotype of the tumors. Although some of the meningiomas had lost or gained one or more chromosomes and had undergone structural aberrations, all of them showed isonucleolinosis, which is normally found only in cells with normal karotype. It seems possible that the occurrence of iso- or anisonucleolinosis is not a specific sign of euploidy or aneuploidy, but of benignity or malignancy of the examined tissue.

Cell Nucleolus↗
PubMed1975

Cytological and cytogenetical studies on brain tumors. VI. No evidence for a translocation in 22-monosomic meningiomas.

The recently detected reciprocal translocations in chronic myeloic leucemia (CML) and Burkitt's lymphoma (BL) made it necessary to clarify if meningiomas really show the described monosomy 22 or also a translocation. In 10 out of 12 meningiomas a total or partial translocation of the missing chromosome 22 to another chromosome could be ruled out by fluorescence banding analysis. Two meningiomas showed marker chromosomes of such a complex composition that it was impossible to decide if a 22 translocation was present or not. From these results it was concluded that meningioma cells, in contrast to CML and BL, show almost regularly a loss of a definitive part of their genome.

Aneuploidy↗
PubMed1975

[Chromosome studies on human fibroblast cultures following exposure to cyproheptadin].

Cyproheptadin was tested on chromosomes in fibroblast cultures of children for its possible mutagenic efficacy. Neither 24-hour exposure to Cyproheptadin at concentrations of 8-16 mg/l medium nor a chronic exposure for 8-11 weeks at a concentration of 8 mg/l caused any discernable chromosomal mutations. At concentrations between 8-16 mg/l reversible cytomorphological and cytochemical changes occured; 32 mg/l suppressed cell division and higher concentrations (64-128 mg/l) brought about irreversible changes leading to cell death. These reactions were rather in vitro cytotoxic effects of unspecific nature.

Cell Division↗
PubMed1975

Cytological and cytogenetical studies on brain tumors. V. Preferential loss of sex chromosomes in human meningiomas.

Twelve out of 88 cytogenetically examined meningiomas of female patients showed, in addition to the typical loss of a chromosome 22, a loss of 1 or more chromosomes of group C. Among them 8 tumors had less than 8% cells with Barr-body-like particles, whereas in one tumor 12% and in 3 others over 20% Barr bodies were found, which, based on control studies, were classified as sex-chromatin negative, partly positive, and positive, respectively. In one case the loss of an X chromosome was verified by Giemsa banding. In 6 out of 24 meningiomas of male origin, the chromosomal morphology and association pattern strongly indicated that besides the loss of a chromosome 22, the Y chromosome was also missing. Moreover, the loss of the male sex chromosome could be ascertained in 4 tumors by the conspicuous absence of Y fluorescence in interphase nuclei and in metaphase plates after fluorescence staining. The findings are discussed in connection with the gonosomal loss in other human tumors and in old age.

Adult↗