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Biomedical subjects

H Zahner

Publications and source records attributed to H Zahner.

108 records · Page 6Linked to original sources
PubMed1979

CELISA (complement-enzyme linked immuno sorbent assay) a new method for the estimation of complement fixing antibodies; its use for Chagas' disease.

A description is given of a new method for the estimation of complement fixing antibodies. This is a modification of the Enzyme Linked Immuno Sorbent Assay (ELISA) to a Complement-Enzyme Linked Immuno Sorbent Assay (CILISA) using an anti-C3 conjugate. The method is independent of the haemolytic system. To test the sensitivity of CELISA, comparative studies were carried out with the Haemolytic Complement Fixation Test (CFT), indirect haemagglutination test and ELISA with sera from patients with Chagas' Disease. The results with CELISA showed significant correlations with CFT titers whereas no correlations were observed with the other serological tests.

Antibodies↗
PubMed1979

Chemotherapeutic studies on Litomosoides carinii infection of Mastomys natalensis. 8. The action of furazolidone on adult worms and microfilariae.

After oral administration of furazolidone in doses of 5 x 50 mg/kg and 1 x 100 mg/kg body weight to Litomosoides carinii--infected Mastomys natalensis microfilaraemia decreased continuously and was reduced by more than 98% 42 days after start of treatment. After the 5-day treatment all adult female and male worms were found dead and encapsulated within 2 weeks, whereas after the single dose 100% of the female parasites were encapsulated 28 days after treatment. In untreated animals quantiative examinations of the intrauterine stages showed an average number of 500 x 103 embryos per adult female worm. Following the 5-day treatment the number of embryos per female parasite was reduced after 42 days to 12.5 x 103, and after the single treatment to 26.9 x 103. By classification into 5 different stages (2- and 4-cell stages, Morula stage, "Horse-shoe" stage, "Ring" and "Brezel" stages, and intruterine microfilariae) an embryogram showed a continuous increase in pathologically-altered embryos during the whole observation period. The 2- and 4-cell stages suffered the most damaged. By 16 days after the end of the 5-day treatment and by 28 days after the single treatment all embryonic stages in the uteri were found to be pathologically altered. Furazolidone possessess high macrofilaricidal activity together with a considerable adverse effect on embryognesis and some delayed effect on microfilaraemia.

Animals↗
PubMed1976

[Capillaria hepatica infection of Mastomys natalensis: on the development, egg production and host reaction (author's transl)].

Studies on the development and egg production of Capillaria hepatica and on the macroscopically visible alterations of the liver and spleen of the host were carried out in experimentally infected Mastomys natalensis. Following the oral administration of infective eggs the first stage larvae hatched in the caecum of fasting and fed animals after about 8 and 15 hours respectively. The prepatency could be found with 20 days. The dynamics and duration of egg production of the parasite proved to be dependent on the infective dose. After the first week of patency increasing numbers of eggs/liver were found with increasing doses of infection up to 800 eggs per animal. This relation could not be observed 76 days post infection. After infections with 50, 300 und 800 eggs per animal maximum egg production was found between 60 and 72, 36 and 48 and about 30 days p.i., respectively. The egg production of the parasites correspondently continued for more than 85 days after infection or had ceased 72 or 48 days p.i. Intraperitoneal administration of infective eggs revealed a lower infection rate, evaluated by the number of eggs per liver, than oral infection.

Age Factors↗
PubMed1975

[Chemotherapeutic studies on litomosoides carinii infection of mastomys natalensis. 5. Alterations of haematological parameters after the administration of filaricidal compounds (author's transl)].

The peripheral blood picture of multimammate rats (Mastomys natalensis) infected with Litomosoides carinii was studied. Changes were followed in patent infections from 64 to 176 days after infection in animals treated with diethylcarbamazine (5 x 100 mg/kg per os), HOE 258 V (5 X 10 mg/kg subcutaneously), suramin (5 x 40 mg/kg subcutaneously) and with a combined treatment of diethylcarbamazine and suramin. In addition, haematological changes were followed in non-infected animals treated with suramin alone as well as in non-infected and infected animals receiving no treatment. During the course of non-treated L. carinii infection in Mastomys natalensis, animals developed anaemia, an increased sedimentation rate and a leucopenia. In addition there was a relative increase in circulating eosinophils, in large lymphocytes and monocytes and a relative decrease in small lymphocytes. After treatment with the microfilaricidal drugs diethylcarbamazine and HOE 258 V blood changes followed those of infection controls but there was a transient change towards normal in peripheral blood values when the microfilariaemia was reduced by the drugs. After treatment with the macrofilaricide suramin, nearly normal values were reached at the end of the experiment for the red blood cell and leucocytes levels. Combined treatment with suramin and diethylcarbamazine resulted in relatively normal levels for some parameters. Suramin was shown to cause haematological changes in normal animals which became evident several days before use of the drug. Such changes added to the pathological alterations caused by the filarial infection. In both infected and non-infected animals suramin treatment resulted in oedema around the nose and on the front feet approximately three weeks afterwards.

Animals↗