Computerized analyzing system for chemiluminescence.
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Biomedical subjects
Publications and source records attributed to H Yuki.
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A high-performance liquid chromatographic procedure for the determination of serum iron is reported. Serum iron extracted with methyl isobutyl ketone was converted to dibenzoylmethane chelate (molecular weight 725), and it was separated from excess dibenzoylmethane (molecular weight 224) by gel permeation chromatography. The chelate was determined by measuring ultraviolet absorption at 280 nm. Good reproducibility, recovery, and correlation with the conventional colorimetric method were observed.
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A method for the determination of 6-methyladenine (6MA) by high-performance liquid chromatography (HPLC) has been developed. DNA bases were separated by using the strong cation-exchange resin Zipax SCX. Purine bases were obtained by hydrolysis and dialysis of DNA and analysed by HPLC. 6MA in DNA from Escherichia coli was determined by the proposed method. It is suggested that the method could be applicable to analyses of 6MA from other biological sources.
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