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Biomedical subjects

H Yoshii

Publications and source records attributed to H Yoshii.

At least 91 records · Page 5Linked to original sources
PubMed1981

[Immunopharmacological actions of neurotropin. (1). Immunostimulating actions of neurotropin (author's transl)].

Neurotropin (NSP) is an extract isolated from vaccinia virus-inoculated and inflamed skin of rabbits. The present study was undertaken to examine the immunostimulating actions of NSP. NSP alone did not induce a proliferation of spleen cells from mice or human peripheral blood lymphocytes (PBL). However, NSP clearly enhanced concanavalin A (Con A)-induced proliferation of both spleen cells and human PBL, but slightly inhibited lipopolysaccharide (LPS)-induced proliferation of spleen cells. NSP reversed the decreases of Con A-induced proliferation of spleen cells pretreated with mitomycin C (MMC) in a dose of 5 microgram/ml or irradiated at 100 to 500 R, while NSP did not reverse the decreases of LPS-induced proliferation of spleen cells pretreated with MMC, in a dose of 5 to 50 microgram/ml, or irradiated at 100 to 1000 R. NSP enhanced in a dose-dependent fashion one-way mixed lymphocytes culture (MLC) reaction using MMC-treated spleen cells from BDF1 mice as stimulator cells and those from C57BL/6 mice as responder cells, respectively. NSP reversed the considerable decrease of MLC-mediated proliferation of responder cells pretreated with MMC, in a dose of 5 to 10 microgram/ml, or irradiated at 100 to 250 R. These results indicate that NSP activates T cell function participating in cell-mediated immunity.

Adjuvants, Immunologic↗
PubMed1981

[Immunopharmacological actions of neurotropin. (2). Effect of neurotropin on graft-versus-host reaction (author's transl)].

It was reported that Neurotropin (NSP) is a new immunostimulating agent which reverses the decrease of proliferation of irradiated spleen cells from mice caused by concanavalin A or specific antigen. The present study was undertaken to examine the possibility that NSP prevents graft-versus-host reaction (GVHR) through host-versus-graft reaction (HVGR) in mice. Allogeneic (C3H/He) spleen cells were performed intravenously into 900 R-irradiated recipient mice (BALB/c). Systemic GVHR was clearly prevented by NSP as well as by cyclophosphamide or prednisolone administered intraperitoneally into irradiated recipient mice. In recipient mice irradiated with 1000 R, a more remarkably systemic GVHR was induced as compared to recipients irradiated at 250 to 500 R. NSP suppressed the proliferation of donor cells at the induction phase of GVHR, whereas NSP did not inhibit both systemic and local GVHRs induced by cell transfer at the effector phase. A decrease in hematopoietic function following irradiation or dosing with immunosuppressive drugs was also reversed by NSP. However, NSP had no effect on increases in hematopoietic function mediated by syngeneic bone marrow transplantation into 900 R-irradiated mice. These results strongly suggest that the inhibition of allogeneic GVHR by NSP is responsible for the suppression of donor cell proliferation by adequately restoring immunologic function in irradiated recipient mice, namely HVGR.

Adjuvants, Immunologic↗
PubMed1979

Tumors of Sprague-Dawley rats induced by long-term feeding of phenacetin.

Carcinogenicity of phenacetin was tested using Sprague-Dawley rats. Two groups of animals containing 50 males and 50 females per group were fed respectively with 2.5% and 1.25% phenacetin diet for 18 months and fed thereafter with basal diet for 6 months. Control animals containing 65 males and 65 females were fed with basal diet for 24 months. Animals surviving more than 24 months were regarded as effective animals and killed. Rats that died of tumor development within 24 months were also regarded effective animals. Every organ from the killed and dead animals was fixed in 10% formaldehyde solution and examined histopathologically. Effective number of rats was 27 males and 27 females in 2.5% phenacetin feeding group, and 22 males and 25 females in 1.25% phenacetin feeding group. In control group, 19 males and 25 females were effective. Neoplasms including spontaneous tumors were detected in 26 out of 27 males (96.3%) and 21 out of 27 females (77.8%) of 2.5% phenacetin feeding group, and in 20 out of 22 males (90.9%) and 19 out of 25 females (76.0%) of 1.25% phenacetin feeding group. In control group, 1 out of 19 males (5.3%) and 6 out of 25 females (24.0%) showed spontaneous tumor development. Histopathologically, carcinomas of the nasal cavity, such as adenocarcinoma, squamous cell carcinoma, and transitional cell carcinoma, and the urinary passage, as renal cell carcinoma of the kidney pelvis, and transitional cell carcinoma of the urinary bladder, were most conspicuous, suggesting the target organs of phenacetin carcinogenesis. Males showed higher tumor incidence compared to females. The higher the concentration of phenacetin given, higher incidence of tumors was observed.

Adenocarcinoma↗
PubMed1977

Production of alpha-fetoprotein by cultured rat ascites hepatoma cells determined in vivo as alpha-fetoprotein-negative.

Three ascites hepatoma cells of the rat, AH-41B, AH-34, and AH-64B, which had been determined in vivo as alpha-fetoprotein-negative, were cultivated in vitro in a medium containing adenosine 3',5'-cyclic monophosphate (cyclic AMP), cyclic dibutyryl-AMP, or theophylline. The concentration of alpha-fetoprotein in culture media was measured by the 125 I-radioimmunoassay. Results demonstrated that the AH-41B cells produced alpha-fetoprotein in vitor, the concentration of which being elevated in the media with three substances, while the remaining AH-34 and AH-64B cells did not. A comment was made on the producibility of alpha-fetoprotein in the so-called alpha-fetoprotein-negative hepatoma cells.

Animals↗
PubMed1976

Alpha-fetoprotein and albumin produced by subclonal cell population of the ascites hepatoma AH-66 in vitro.

Subclonal cell populations were prepared from a clonal line of the rat ascites hepatoma AH-66 in vitro, using the agar plate culture method. alpha-Fetoprotein and albumin concentrations in culture media of these subclones were determined by 125I-radioimmunoassay and single radial immunodiffusion method, respectively. Results demonstrated that the AH-66 clone was a complex of cells with varying producibility of alpha-fetoprotein and albumin. All the subclones showed varied and distinct production of alpha-fetoprotein, but not all the subclones produced detectable levels of albumin.

Albumins↗
PubMed1976

[Trichobezoar].

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Adolescent↗
PubMed1975

Intestinal tumors of rats by gastric or intestinal administration of cycad extract and cycasin.

Sprague-Dawley rats were given gastric intubation of cycad extract (group 1), rectal infusion of cycasin (group 2), or rectal indusion of cycad extract after external colostomy at 1/3 proximal portion of the large intestine (group 3). In group 1, intestinal tumors developed in any portion of the intestinal tract ranging from the duodenum to the rectum. In group 2, tumors developed in mucosa of the large intestine. In group 3, however, tumors arose from both sites of intestinal mucosa which were in contact and not in contact directly with the cycad extract infused. Possible hypothesis for intestinal tumor development by cycad extract and cycasin was presented.

Adenocarcinoma↗