Utilization of 13C-13C coupling in structural and biosynthetic studies. XI Biosynthetic studies of coarctatin.
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Biomedical subjects
Publications and source records attributed to H Yonehara.
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The amino-acid composition and molecular weight of actinocarcin, and C-terminal structure of actinocarcin are reported in this paper. By STEIN-MOORE'S and ultraviolet-absorption methods, the molar ratio of amino acids in actinocarcin was determined. The N-terminal amino acids are alanine and valine. The C-terminal amino acid, determined by hydrazinolysis, is tryptophan. The molecular weight is about 11,000 as estimated by ARCHIBALD'S method and amino acid analysis. Three fragments, named BrCN-I, BrCN-II and BrCN-III, were obtained by cyanogen bromide cleavage. The structure of BrCN-III, namely the C-terminal structure of actinocarcin was determined by EDMAN degradation.
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A new antifungal antibiotic was isolated from the fermentation broth of Streptomyces sp. 5140-A1. Degradation studies of the crystalline antibiotic, m.p. 186 approximately 188 degrees C, C21H28O8N2, suggested Piricularia oryzae and less toxicity against killfish than antimycin A--blastmycin antibiotics.
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