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Biomedical subjects

H Yeager

Publications and source records attributed to H Yeager.

At least 55 records · Page 3Linked to original sources
PubMed1978

Human alveolar macrophages: antigen-independent binding of lymphocytes.

To characterize the initial step in alveolar macrophage (AM)-lymphocyte (L) interaction in the human lung, we studied the ability of human AM to bind autologous blood L in vitro in the absence of antigen. AM were obtained by saline bronchial lavage through a fiberoptic bronchoscope. Monolayers of AM attached to glass bound autologous blood L prepared by Ficoll-Hypaque and nylon wool column separation. The AM-L binding increased from zero time to a maximum at 2 h and then declined to a zero time value at 18 h. The binding was dependent on the number of L added to the AM monolayers, with greatest binding at an AM:L ratio of 1:50. AM:L binding required viability of AM, but not of L, and was temperature dependent. Pretreatment of AM with iodoacetic acid, trypsin, neuraminidase, or colchicine diminished attachment of L at 2 h. Neuraminidase pretreatment of L resulted in increased binding to nontreated AM. Thus, a physical interaction between human AM and autologous peripheral blood L can occur in vitro in the absence of known antigen; similar interaction in vivo may play a role in the generation of cell-mediated immune responses in the lung.

Adult↗
PubMed1977

Rickets in children receiving anticonvulsant drugs. Biochemical and hormonal markers.

Forty-one epileptic children, aged 2 to 16 years, receiving combinations of phenobarbital, phenytoin, and primidone, and 39 control children were studied. The epileptics demonstrated slight but significant reductions in serum calcium, phosphorus, and 25-hydroxyvitamin D concentrations, and a significant increase in serum alkaline phosphatase values. No significant difference in serum immunoreactive parathyroid hormone levels was noted. Further analysis of the data revealed that patients whose drug therapy included primidone had the lowest serum levels of calcium and 25-hydroxyvitamin D. They had also received the largest number of drugs for the longest duration, and had serum phenobarbital levels that were significantly higher than those of other patients. The minimal degree of vitamin D deficiency in our epileptic children contrasts with the results of other investigations and warrants emphasis. The reasons for this difference are not apparent.

Adolescent↗
PubMed1977

Sarcoidosis: analysis of cells obtained by bronchial lavage.

The cellular content of bronchial lavage was studied in 14 patients with sarcoidosis and 20 normal volunteers. There was no alteration in the total number of cells recovered, except for the expected increase in cellularity in smokers. Nonsmoking patients with sarcoidosis had 19.6 per cent lymphocytes, compared to 8.1 per cent lymphocytes in nonsmoking control subjects. The percentage of alveolar macrophages showing spontaneous adherence of one or more bronchial lymphocytes were, respectively, 10.8 and 8.4 in nonsmoking and smoking patients with sarcoidosis, compared to 1.8 and 2.1, respectively, in nonsmoking and smoking control subjects.

Adult↗
PubMed1976

Idiopathic pulmonary hemosiderosis: ultrastructural studies and responses to azathioprine.

Two boys are presented who fulfilled criteria for a diagnosis of idiopathic pulmonary hemosiderosis. A lung biopsy specimen from the first patient showed alveolar-capillary basement membrane abnormalities, together with abnormalities of capillary endothelial cells and hemosiderin-laden macrophages. A lung biopsy specimen from the second patient showed mainly capillary endothelial abnnormalities and interestitial fibrosis. Both patients had a noticeable improvement in symptoms and relative stabilization of their roentgenographic and pulmonary function abnormalities following azathioprine therapy.

Anemia, Hypochromic↗
PubMed1976

Asthma: comparative bronchodilator effects of ipratropium bromide and isoproterenol.

The effect of ipratropium bromide administered at two dosage levels, 40 and 80 mug, isoproterenol, 150 mug, and placebo using a metered dose inhaler was evaluated in ten adult patients with asthma in a double-blind, crossover study. The new atropine-like drug proved to be as effective a bronchodilator as isoproterenol in this study, although it had a later peak effect. Ipratropium bromide had a longer course of action than isoproterenol (4 hours compared to 1-2 hours) and was free of significant side effects. The larger dose of the new drug produced a slightly greater and longer-acting effect than the smaller dose. Ipratropium bromide seems to have had bronchodilator effects on both large and small airways.

Adult↗
PubMed1976

Comparative production of interferon by explanted lymphoreticular tissue and alveolar macrophages from rabbits and humans.

Studies were undertaken to compare interferon production among a variety of lymphoreticular cells, with emphasis on the alveolar macrophage. Explanted cells from rabbit lung, spleen, peritoneum, bone marrow, and blood produced interferon in varying amounts in response to six of the seven viruses studied. The various lymphoreticular tissues responded differently to a single interferon-inducing virus, and each tissue produced varying amounts of interferon when stimulated by different viruses. In addition, glass-adherent rabbit alveolar macrophages produced more interferon than did the nonadherent subpopulation. Human blood and lung cells produced much less interferon than did the equivalent rabbit cells under similar conditions of stimulation. It appeared that interferon production may have been controlled by several variables, including the species, the type of inducer, and the type of tissue and cell.

Adult↗
PubMed1975

Nephrectomy in selected patients with severe refractory hypertension receiving dialysis.

The effect of bilateral nephrectomy was examined in ten patients with severe hypertension in whom dialysis and multiple drug therapy had not resulted in blood pressure control and was compared with that in 15 patients undergoing nephrectomy solely in preparation for transplantation. A highly significant, P less than 0.0005, and clinically impressive reduction in mean blood pressure occurred in the first group of patients, permitting discontinuation of all drugs. Although impossible to quantitate, these patients also demonstrated a reversal of a failure-to-thrive syndrome which had seriously compromides prenephrectomy management. Nephrectomy resulted in a reduction in mean hematocrit values of greater than 20 per cent and less than or equal to 20 percent showed a significant fall in the former but not in the latter. No increase in transfusion requirements developed in these patients. Until more potent and reliable medical therapy becomes generally availabe, nephrectomy will remain an important treatment modality in those patients requiring dialysis and having severe unremitting hypertension.

Antihypertensive Agents↗
PubMed1974

Pinocytosis by human alveolar macrophages. Comparison of smokers and nonsmokers.

Alveolar macrophages were obtained from human volunteers, smokers and nonsmokers, by bronchial lavage through a fiberoptic bronchoscope. Cells were incubated in a chemically defined medium containing [(14)C]sucrose (0.36 mM) and varying concentrations of rabbit serum. Pinocytosis was assessed by the cellular uptake of isotope over 30, 75, and 120-min periods. Pinocytic activity of smokers' cells was dependent on serum concentration but always less than the activity of nonsmokers' cells. The degree of pinocytosis by nonsmokers' cells was independent of serum concentration. It is concluded that the decreased level of pinocytic activity in smokers' alveolar macrophages as indicated by the uptake of sucrose in the presence of rabbit serum may represent a form of reticuloendothelial blockade.

Acid Phosphatase↗