Improved method for determination of "true" creatinine.
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Biomedical subjects
Publications and source records attributed to H Yatzidis.
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Cyanide and thiocyanate was measured in blood and in peritoneal lavage solution in patients with advanced or terminal renal failure. The behavior of these substances was comparable to that of normal people. Our finding does not support the hypothesis of an indirect toxicity of urea via its previous conversion to cyanate. Cyanate ion has not been implicated in the toxic status of chronic uraemia.
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Total urinary protein is rapidly precipitated at room temperature by tannic acid. The tannic acid/protein precipitate, dissolved in aqueous triethanolamine/ferric chloride solution, gives a purple-violet color of high absorptivity. Absorbance at 510 nm is linearly related to concentration from 0.05 to 1.50 A for a protein content of 0.05 to 1.50 g/liter, and less than 5 mg/liter can be detected. The CV and analytical recovery ranged from 0.5 to 1.8% and 98 to 103%, respectively. Nonprotein urinary constituents do not interfere.
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