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H Yasunaga

Publications and source records attributed to H Yasunaga.

26 records · Page 2Linked to original sources
PubMed1992

[The changes of the cell-mediated immunity in patients with administration of recombinant erythropoietin].

The change of cell-mediated immunity was studied in patients receiving open heart surgery with or without administration of recombinant erythropoietin (rEPO). Group I was not administered rEPO in 30 patients, and Group II was done intravenously with 200U/kg/day of rEPO for 6.4 +/- 2.4 days before operation and also for 7.2 +/- 3.6 days after operation in 20 patients. The ratio of reticulocyte increased in all patients receiving rEPO. In both groups the ratios of OKT3 and OKT4 positive T lymphocytes decreased significantly on postoperative day 1. However, the ratios in patients with rEPO increased more significantly than in those without rEPO. Lymphocyte blast formation which was indicated by PHA-SI (phytohemagglutinin stimulation index) increased after administration of rEPO. The postoperative PHA-SI in both groups showed similar changes. The level of interleukin-2 (IL-2) production increased after the administration similar to PHA-SI change. The level of it decreased on postoperative day 1 and increased on postoperative days 3 and 7. We administered 200U/kg/day of rEPO for 7 days in a patient with postoperative erythroderma after open heart surgery and the level of IL-2 production was found to also increase in patient according with recovery of symptom. In conclusion, our data suggested that the rEPO might effect on not only erythrocyte but also lymphocyte activation.

Adult↗
PubMed1985

Amino acid sequence of polypeptide chain IIB of extracellular hemoglobin from the polychaete Tylorrhynchus heterochaetus.

The giant extracellular hemoglobin from the polychaete Tylorrhynchus heterochaetus consists of two types of subunits: a "monomeric" chain (chain I) and a disulfide-bonded trimer of chains IIA, IIB, and IIC. The complete amino acid sequence of chain IIB was determined. This chain has 148 amino acid residues and a molecular weight of 17,236 including a heme group. Of the residues in chain IIB, 74 (50%) and 34 (30%) were found to be identical with those in the corresponding positions in Tylorrhynchus chains IIC and I, respectively (Suzuki, T., Furukohri, T., and Gotoh, T. (1985) J. Biol. Chem. 260, 3145-3154). Marked differences were found between the chains of Tylorrhynchus and Lumbricus in the COOH-terminal regions. Significant differences were predicted between the monomeric chain I and the "trimeric" chains (IIB and IIC) in the hydropathy profiles and alpha-helical contents.

Amino Acid Sequence↗
PubMed1984

[Experimental study on free muscle transplantation--histological changes and functional recovery of transplanted muscles in relation to the duration of ischemia and denervation].

UNLABELLED: The purpose of this study was to clarify the influence of temporary ischemia and denervation on the characteristics of transplanted muscles. MATERIALS AND METHODS: The rectus femoris brevis muscle of white rabbit was used. Under an operating microscope, this muscle was mobilized preserving its feeding vessels, and the innervating nerve was severed at 1 cm proximal to the entrance of the muscle. Six groups were made as follows. Group D: The severed nerve was left as it was. Group S: The nerve was sutured immediately after severance. No treatment was made on the feeding vessels. Group SI: The artery and vein were clamped for 1, 2, 3 and 4 hours (group SI-1, 2, 3 and 4) after nerve repair. The proximal and distal tendon were reattached to their original place in every group. Examination I: Transplanted muscles were examined histologically and histochemically from 48 hours to 6 months. Motor end-plate was one of the targets of observation. RESULTS: Main patho-histological changes observed on the muscle and motor end-plates at one week were thought due to denervation, and the longer ischemic time caused more severe change in the group SI-3 and 4. Phagocytosis, destruction of muscle fibers and edema in the interstitial tissue were seen in these long ischemic time groups. A few motor end-plates in the group SI-3 and many of them in the group SI-4 were atrophic and their acetylcholinesterase (AChE) activity was low. Reduced nicotinamide-adenine dinucleotide-tetrazolium reductase (NADH-TR) and phosphorylase activity were reduced in all groups at one week. At 3 weeks, muscle atrophy was pronounced and was found earlier in type II fibers than in type I as a result of denervation. Pathological changes such as elongation, atrophy or segmentation and low AChE activity were observed in the motor end-plates of all groups at 3 and 6 weeks. These findings were more pronounced in the group SI-3 and 4. In all groups at 6 weeks, phosphorylase activity was found to be generally low but NADH-TR reaction stained a few regenerated muscle fibers. Although muscle atrophy was still observed at 9 weeks, recovery in size and shape and histochemical reaction was seen in all groups at this stage. These findings became more manifest at 15 weeks and almost normal appearance of muscle fibers was observed at 6 months. Interstitial connective tissue, however, increased, and necrotized muscle fibers were seen extensively in the group SI-3 and 4.(ABSTRACT TRUNCATED AT 400 WORDS)

Acetylcholinesterase↗
PubMed1969

[Neurosis].

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Humans↗