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Biomedical subjects

H Yasueda

Publications and source records attributed to H Yasueda.

99 records · Page 6Linked to original sources
PubMed1983

Changes of alpha 1- and beta-adrenergic and cholinergic muscarinic receptors guinea pig lung sensitized with ovalbumin.

The effect of immunization of guinea pigs with ovalbumin on the number and affinity of alpha 1- and beta-adrenergic and cholinergic muscarinic receptors was studied in lung membranes by direct binding techniques using 3H-prazosin, 1-3H-dihydroalprenolol and 1-3H-quinuclidinyl benzilate. After immunization by intraperitoneal injection of ovalbumin to guinea pigs, the number and affinity of each receptor in sensitized animals were not significantly different from those of control animals. Sensitization of guinea pigs by an aerosol exposure with the antigen resulted in a decreased number of beta-adrenergic receptor (458 +/- 29 vs. 687 +/- 56 fmol/mg protein; p less than 0.01), and an increased number of alpha 1-adrenergic receptor (36 +/- 2 vs. 25 +/- 2 fmol/mg protein; p less than 0.01), but no change was observed in the number of muscarinic receptors, as compared with control animals. On the other hand, following a prolonged sensitization of guinea pigs with a low dose of the aerosolized antigen, the number of muscarinic receptors was significantly increased in the lung of sensitized animals (50 +/- 2 vs. 42 +/- 2 fmol/mg protein; p less than 0.01); however, we found no significant differences between sensitized and normal animals in the number of alpha 1- and beta-adrenergic receptors. There were no different changes in the affinity of these receptors in all experiments.

Aerosols↗
PubMed1982

Effect of Bordetella pertussis on alpha 1 and beta-adrenergic and cholinergic muscarinic receptors in guinea pig lung membranes.

After intraperitoneal injection of Bordetella pertussis vaccine to guinea pigs, the alpha 1- and beta-adrenergic and cholinergic muscarinic receptors of the whole lung were measured by binding assays with the radioisotope-labeled antagonists, 3H-prazosin, 1-(3)H-dihydroalprenolol and 1-(3)H-quinuclidinyl benzilate, respectively. Injection of guinea pigs with pertussis vaccine resulted in an increase in the maximum binding (Bmax) of 3H-prazosin, while there was about a 30% reduction in 1-(3)H-dihydroalprenolol binding to beta-adrenergic receptors. No difference was observed in the dissociation constant (KD) for binding of each ligand to alpha 1- and beta-adrenergic receptors. 1-(3)H-quinuclidinyl benzilate binding indicated that the Bmax and KD for cholinergic muscarinic receptor in pertussis-sensitized guinea pigs were not significantly different from those normal control animals.

Animals↗
PubMed1980

Simultaneous determination of histamine and N tau-methylhistamine in human plasma and urine by gas chromatography--mass spectrometry.

A new and sensitive method is described for the determination of histamine and N tau-methylhistamine in human plasma and urine by gas chromatography--mass spectrometry. 15N2-Labeled histamine and N tau-[methyl-d3]methylhistamine were used as internal standards. Histamine and N tau-methylhistamine were converted to the derivatives N alpha-heptafluorobutyryl-N tau-ethoxycarbonylhistamine and N alpha-heptafluorobutyryl-N tau-methylhistamine, respectively. After these derivatives had been purified on a small column packed with CPG-10, the molecular ions were monitored during selected ion monitoring. Linear standard curves were obtained in the range of 0.5-10 ng/ml for both compounds. The reliability of the histamine analysis was demonstrated by using two different ion pairs, while a comparison with results from two different derivatizations on the same urine sample also established the specificity of the N tau-methylhistamine analysis. An increase of 1 ng of histamine in the plasma could be precisely determined by the present method. The histamine content of plasma from five normal subjects was determined as 0.83 +/- 0.37 (S.D.) ng/ml and the N tau-methylhistamine content in most subjects was below the limits of this measurement. High excretion of histamine was noted in the urine collected in the early morning from a patient with nephritis.

Gas Chromatography-Mass Spectrometry↗
PubMed1980

Quantitative analysis of histamine in biological samples by gas chromatography--mass spectrometry.

A mass fragmentographic method for the quantitative analysis of histamine in the supernatant from antigen-challenged leukocytes, whole blood, and urine is described. Histamine labeled with two 15N atoms was synthesized and added to the sample as an internal standard. N alpha-Heptafluorobutyryl-N tau-ethoxycarbonylhistamine was prepared for mass fragmentographic analysis and the molecular ions at m/z 379 and 381 were used for monitoring histamine and 15N2-labeled histamine, respectively. The quantitation limit of histamine was 2 ng by this method. The experimental error of the method was less than 7% at the level of 5 ng in the supernatant from antigen-challenged leukocytes. The value obtained by this method correlated well with that from radioisotopic enzymatic assay (r=0.990).

Gas Chromatography-Mass Spectrometry↗