Biomedical subjects

H Yanagi

Publications and source records attributed to H Yanagi.

At least 181 records · Page 10Linked to original sources

PubMed1990

Effect of linoleic acid hydroperoxide on adenovirus DNA replication.

The effect of linoleic acid hydroperoxide on replication of adenovirus type 2 DNA in HeLa cells was examined. The level of the DNA was extremely reduced when the cells immediately after the infection with adenovirus type 2 were incubated for 24 h in the presence of 1 nmol/ml (measured by the thiobarbituric acid reaction and expressed in terms of malondialdehyde) of the hydroperoxide. The levels of the messenger RNA for adenovirus type 2 DNA polymerase and the expressed polymerase were equally reduced. The inhibition of DNA replication by lipid hydroperoxides in animal cells was discussed in relation to degenerative disorders.

Adenoviruses, Human↗

PubMed1989

Cloning and nucleotide sequence of a flagellin-coding gene (hag) from Serratia marcescens 274.

The pigmented enterobacterium Serratia marcescens shows a high frequency of color variation. In strain 274, color variation was shown to be closely associated with flagellar variation [Paruchuri and Harshey, J. Bacteriol. 169 (1987) 61-65]. As a first step towards understanding the genetic basis of this phenomenon, we have cloned and sequenced a flagellin-coding gene of S. marcescens 274. The gene codes for a protein product of 351 aa, with a predicted Mr of 36,862. Amino acid sequence comparisons reveal a high degree of homology between the N-terminal half and C-terminal third of the Serratia flagellin and flagellins from Escherichia coli and Salmonella typhimurium.

Amino Acid Sequence↗

PubMed1989

Expression of human erythropoietin cDNA in human lymphoblastoid Namalwa cells: the inconsistency of a stable expression level with transient expression efficiency.

Recombinant plasmids for the expression of human erythropoietin (EPO) cDNA in Namalwa cells were constructed. From the results of the EPO expression efficiency in transiently transfected cells, it was found that the simian virus 40 (SV40) early promoter directs EPO synthesis more efficiently in Namalwa cells than does the long terminal repeat promoter of Rous sarcoma virus and that the 3'-noncoding sequence including splice junction and polyadenylation site derived from the rabbit beta-globin gene are more effective than those of the SV40 early gene. However, in stable transformants, no simple relationship was found between the expression level of EPO cDNA and the structure of the introduced expression vectors.

Base Sequence↗

PubMed1989

Three novel partial deletions of the low-density lipoprotein (LDL) receptor gene in familial hypercholesterolemia.

The low-density lipoprotein (LDL) receptor genes from 18 unrelated Japanese heterozygotes and 1 homozygote with classical familial hypercholesterolemia were analyzed by Southern blot hybridization using fragments of the human LDL receptor cDNA as probes. Four different deletion mutations were detected among 20 mutant LDL receptor genes (20%); they were characterized by restriction mapping. None of these mutations has previously been reported in Caucasian patients with FH: three of the mutations were novel and one was similar to the deletion mutation of FH-Tonami described previously in Japanese patients. In three of the four deletion mutations, the rearrangements were related to intron 15 of the LDL receptor gene, in which many Alu sequences exist. The data suggest that a wide range of molecular heterogeneity exists even in major rearrangements resulting in deletions in the LDL receptor gene. The data also support the hypothesis that there are preferential sites within the LDL receptor gene for major rearrangements resulting in deletions. The possibility that a higher frequency of deletion mutations occurs in classical FH than previously suspected is discussed.

Blotting, Southern↗

PubMed1989

An ultrasonographic method for detection of Achilles tendon xanthomas in familial hypercholesterolemia.

The diagnosis of familial hypercholesterolemia (FH) is frequently made on clinical grounds and detection of tendon xanthomas is crucial for that. In order to clarify whether ultrasonography (US) can be used as a reliable and practical method for detection of Achilles tendon xanthomas in FH, the Achilles tendon thickness in the sagittal section was examined by US in 15 patients with heterozygous FH and 34 normocholesterolemic subjects. US visualized clearly the anterior and posterior borders of the Achilles tendon. The Achilles tendon thickness determined by US correlated with that measured by conventional radiography (r = 0.99). The mean values +/- SD of the Achilles tendon thickness determined by US were 4.5 +/- 0.5 mm in the normal controls and 11.9 +/- 5.1 mm in the patients and the difference was significant (P less than 0.001). In 13 of 15 patients, US visualized thickened Achilles tendons with convex shape in the sagittal section. All the thickened Achilles tendons revealed by US were confirmed by radiography. The data indicate that US can detect Achilles tendons thickened by xanthomas. We conclude that US is a useful aid in the clinical diagnosis of FH.

Achilles Tendon↗

PubMed1989

[Preoperative perspective versus postoperative functional assessment of surgery in chronic mitral regurgitation].

Thirty patients with chronic pure mitral regurgitation who underwent mitral valve replacement during 1981 to 1986 were evaluated for pre- and one year postoperative left ventricular function using two dimensional echogram. Indicators in their evaluation were DdI, DsI, MassI and systolic and diastolic R/Th. Postoperatively, patients (n = 7) were placed in a group of abnormal responses if even one of these indicators of left ventricular function became worse than preoperative performance, patients in this group were symptomatic and two subsequently died. The postoperative FS and mVcf data fort this group were significantly lower than the patients with normal response group (n = 23). These two groups could not be differentiated by preoperative assessments of either DdI, MassI, systolic/diastolic T/Th or normalized systolic/diastolic peak velocity. However, a combination of normalized systolic peak velocity lower than 1.70 and diastolic peak velocity lower than 2.20 of left ventricular wall motion proved to be good predictors of postoperative results.

Chronic Disease↗

PubMed1988

TaqI polymorphism in the LDL receptor gene and a TaqI 1.5-kb band associated with familial hypercholesterolemia.

The low density lipoprotein (LDL) receptor gene was analyzed in 67 unrelated healthy Japanese and 38 members of six consecutive families with familial hypercholesterolemia (FH) by Southern blot hybridization with TaqI, an LDL receptor cDNA fragment containing exons 1 to 8 being used as a probe. A new TaqI RFLP at the LDL receptor locus was detected with allele frequencies of 0.67 and 0.33. The data obtained with smaller cDNA subfragment probes revealed that the TaqI RFLP site is located within 1.1 kb of the 5' side of the EcoRI site of exon 5. The TaqI RFLP was in linkage disequilibrium with the PstI RFLP but showed no significant linkage disequilibrium with the RFLPs for AvaII, ApaLI/I15, PvuII, NcoI, and ApaLI/3'. Among the seven RFLPs at the LDL receptor locus, the TaqI RFLP was the only useful genetic marker in one of the six families with FH. Furthermore, the association of an additional TaqI 1.5-kb band with a mutant LDL receptor gene was observed in another family with FH in which the proband was homozygous for all of the seven RFLPs. The data obtained with various restriction enzymes and smaller cDNA subfragments probes suggested that a minor change in nucleotide sequences in the region including exons 5 to 8 is present in the mutant gene. These data suggest that the TaqI RFLP is a useful genetic marker at the LDL receptor locus and that TaqI serves for the analysis of some mutant LDL receptor genes, when used with small LDL receptor cDNA probes.

DNA Restriction Enzymes↗

PubMed1988