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Biomedical subjects

H Y Chen

Publications and source records attributed to H Y Chen.

At least 73 records · Page 4Linked to original sources

WAIS-III reliability data for clinical groups.

Reliability estimates for psychological tests are almost always reported for nonclinical populations (e.g., the normative samples). Such practice will no longer be sufficient as the new standards for testing call for an adequate assessment of psychometric properties within the specific population being tested. The purpose of this study was to provide internal consistency reliability estimates for clinical groups on the Wechsler Adult Intelligence Scale-Third Edition. The study included data from 403 clinical participants composed of 10 groups of adults recruited as part of the WAIS-III clinical validity studies. Split-half reliability coefficients were obtained for these groups replicating the procedure used in the WAIS-III. With 8 of the clinical groups, the split-half reliability coefficients were comparable to, or even higher than, those reported for the WAIS-III standardization sample. In general, the split-half coefficients for the Verbal subtests tended to be higher than the coefficients for the Performance subtests. The high magnitude and general pattern of these coefficients demonstrate that the WAIS-III scales do not include additional error variance above and beyond what is reported in the WAIS-III-WMS-III Technical Manual when it was used to assess certain clinical groups. For the ADHD/ADD and learning disabilities groups, however, the internal consistencies coefficients of some subtests were relatively lower, although not statistically significant, than the normative sample. These findings may reflect more heterogeneity within the groups. The implications for assessment and for using alternate methods of determining the psychometric properties in these populations are discussed.

Adolescent↗

Human milk antibacterial factors: the effect of temperature on defense systems.

Bovine milk will eventually spoil at refrigeration temperatures, but endogenous or exogenous pathogenic or spoilage bacteria in human milk stored for delayed feeding will die. We investigated the mechanism for these antibacterial properties and their response to high-tempertature, short-time (HTST, 72 degrees C-75 degrees C, 15 sec) and low-temperature long-time (LTLT, 65 degrees C, 30 min) pasteurization. Nonpathogenic Listeria innocua (10(6) cfu/mL) was inoculated into raw and processed bovine and human milk; bacterial plate counts twice weekly determined antibacterial activities. Up to 99% of L. innocua were killed and further growth was inhibited in raw and pasteurized human milk for at least 60 days at 4 degrees C. Reactive IgA antibodies against Listeria antigens were demonstrated by enzyme immunoassay in some human milk samples; sIgA activity against Escherichia coli O antigens was significantly decreased by heat treatments (raw, 1.8; HTST, 1.1; LTLT, 1.3 activity units). Adding human lactoferrin (0.5-20 mg/mL) to the Listeria inoculum (approximately 10(7) cfu/mL) in 1% peptone water did not inhibit bacterial growth.

Animals↗

Shear instabilities of freely standing thermotropic smectic- A films.

In this Letter we discuss theoretically the instabilities of thermotropic freely standing smectic- A films under shear flow [3]. We show that, in Couette geometry, the centrifugal force pushes the liquid crystal toward the outer boundary and induces smectic layer dilation close to the outer boundary. Under strong shear, this effect induces a layer buckling instability. The critical shear rate is proportional to 1/sqrt[d], where d is the thickness of the film.

Journal Article↗

Mouse mammary tumor virus-Ki-rasB transgenic mice develop mammary carcinomas that can be growth-inhibited by a farnesyl:protein transferase inhibitor.

For Ras oncoproteins to transform mammalian cells, they must be posttranslationally modified with a farnesyl group in a reaction catalyzed by the enzyme farnesyl:protein transferase (FPTase). Inhibitors of FPTase have therefore been developed as potential anticancer agents. These compounds reverse many of the malignant phenotypes of Ras-transformed cells in culture and inhibit the growth of tumor xenografts in nude mice. Furthermore, the FPTase inhibitor (FTI) L-744,832 causes tumor regression in mouse mammary tumor virus (MMTV)-v-Ha-ras transgenic mice and tumor stasis in MMTV-N-ras mice. Although these data support the further development of FTIs, it should be noted that Ki-ras is the ras gene most frequently mutated in human cancers. Moreover, Ki-RasB binds more tightly to FPTase than either Ha- or N-Ras, and thus higher concentrations of FTIs that are competitive with the protein substrate may be required to inhibit Ki-Ras processing. Given the unique biochemical and biological features of Ki-RasB, it is important to evaluate the efficacy of FTIs or any other modulator of oncogenic Ras function in model systems expressing this Ras oncoprotein. We have developed strains of transgenic mice carrying the human Ki-rasB cDNA with an activating mutation (G12V) under the control of the MMTV enhancer/promoter. The predominant pathological feature that develops in these mice is the stochastic appearance of mammary adenocarcinomas. High levels of the Ki-rasB transgene RNA are detected in these tumors. Treatment of MMTV-Ki-rasB mice with L-744,832 caused inhibition of tumor growth in the absence of systemic toxicity. Although FPTase activity was inhibited in tumors from the treated mice, unprocessed Ki-RasB was not detected. These results demonstrate the utility of the MMTV-Ki-rasB transgenic mice for testing potential anticancer agents. Additionally, the data suggest that although the FTI L-744,832 can inhibit tumor growth in this model, Ki-Ras may not be the sole mediator of the biological effects of the FTI.

Alkyl and Aryl Transferases↗

Identification and analysis of the sap genes from Vibrio fischeri belonging to the ATP-binding cassette gene family required for peptide transport and resistance to antimicrobial peptides.

Partial nucleotide sequences of the sapD and sapF genes of the sap operon (GenBank Accession No. AF178651) from Vibrio fischeri ATCC 7744 have been determined, and the peptide transport system of ATP-binding proteins SapD and SapF encoded by the genes have been deduced. Alignment and comparison of the Sap proteins of V. fischeri, Escherichia coli, Salmonella typhimurium, and Haemophilus influenzae Rd show that these proteins are homologous. The sap operon residing in the genome enables V. fischeri to transport peptides and resist antimicrobial peptides. Nucleotide sequence and functional analyses confirm that the specific regulatory-region-like sequence R&R* that resides inside the sapD gene and before the sapF gene functions in gene expression and regulation; also, it is regulated by the LuxR-AI complex of the V. fischeri lux regulon. The putative upstream activator binding sequences SigmaUASI, SigmaUASII, SigmaUASIII TGTCGACTTGGGCCTCGCTGTCCGTATGCACA (72nd to 103rd bp), TGTCCGTATGCACA (90th to 103rd bp), and TGTTCAAGTACCAGAAAGACA (111st to 133rd bp) in the R&R* sequence, which are similar to the two-component regulator binding sequence TGT-N(8-12)-ACA and the LuxR-AI binding sequence ACCTGTAGGATCGTACAGGT in the regulatory region of the V. fischeri lux regulon, might be the specific sequences recognized by the LuxR-AI complex for enhancement.

Amino Acid Sequence↗

Structure-activity relationship studies on 1-[2-(4-Phenylphenoxy)ethyl]pyrrolidine (SC-22716), a potent inhibitor of leukotriene A(4) (LTA(4)) hydrolase.

Leukotriene B(4) (LTB(4)) is a pro-inflammatory mediator that has been implicated in the pathogenesis of a number of diseases including inflammatory bowel disease (IBD) and psoriasis. Since the action of LTA(4) hydrolase is the rate-limiting step for LTB(4) production, this enzyme represents an attractive pharmacological target for the suppression of LTB(4) production. From an in-house screening program, SC-22716 (1, 1-[2-(4-phenylphenoxy)ethyl]pyrrolidine) was identified as a potent inhibitor of LTA(4) hydrolase. Structure-activity relationship (SAR) studies around this structural class resulted in the identification of a number of novel, potent inhibitors of LTA(4) hydrolase, several of which demonstrated good oral activity in a mouse ex vivo whole blood assay.

Administration, Oral↗

Simultaneous determination of polycarboxylic acids by capillary electrophoresis with a copper electrode.

The simultaneous determination of polycarboxylic acids including oxalic acid, citric acid, malonic acid, malic acid, tartaric acid, aspartic acid and glutamic acid was achieved by capillary electrophoresis with a copper disk electrode (d = 200 microm). In the system. 0.2 mmol/l cetylpridinium bromide (CPB) was used as an electroosmotic flow (EOF) modifier to reverse the direction of EOF. The effects of the solution pH and CPB concentration on separation were evaluated to achieve the optimum separation conditions. At the working potential of +0.14 V (vs. saturated calomel electrode), the calibration curves for all polycarboxylic acids studied were linear with 2 approximately 3-orders of magnitude and all the detection limits (S/N = 3) were below 15 fmol except malonic acid. Furthermore, the oxalic and citric acids in urine were successfully separated and determined with high sensitivity.

Carboxylic Acids↗

Mechanical properties of new composite restorative materials.

OBJECTIVE: Determination of flexural strength, flexural modulus, fracture toughness, Vickers hardness, and wear resistance of condensable composites (Solitaire, Surefil, Alert) and an ormocer (Definite) in comparison with a hybrid composite (Tetric Ceram) and an ion-releasing composite (Ariston pHc). METHODS: Flexural strength, flexural modulus, and fracture toughness were determined in 3-point bending. Single-edge notched-bend specimens were used to evaluate fracture toughness. Microhardness was measured with a Vickers indenter. Wear was determined in a pin-on-block-design with a Degusit antagonist at 50 N load and quantified by a replica technique after 6000, 10000, 30000, and 50000 load cycles using a 3D-laser scanner. All results were statistically analyzed with ANOVA and post hoc Tukey HSD tests. RESULTS: Alert exhibited the highest flexural modulus, K(IC), and hardness, but lowest wear resistance. Solitaire presented the highest wear resistance, but significantly lower flexural strength, flexural modulus, K(IC), and hardness than all other materials. No significant correlation could be detected between hardness and wear of the tested composites with Pearson's correlation coefficient. SIGNIFICANCE: The condensable composites differed significantly in their mechanical properties. This study suggested that, besides the filler content level and filler size, other factors like matrix-filler interactions highly influence the fracture and wear behavior of the materials.

Composite Resins↗

Immunomodulation by dietary beta-1, 3-glucan in the brooders of the black tiger shrimp Penaeus monodon.

The present study evaluated the effectiveness of beta-1,3-glucan derived from Schizophyllum commune in enhancing shrimp survival as well as haemocyte phagocytosis and superoxide anion production in brooder Penaeus monodon. Pond-reared P. monodon adults (135 +/- 25 g) stocked in outdoor or indoor tanks were fed either a test diet containing beta-1,3-glucan (2.0 g kg(-1) or a glucan-free control diet for 40 days. Their survival was compared. The brooders reared in indoor tanks were analysed at days 0, 1, 3, 6, 12, 24, 30 and 40 for their haemocyte phagocytic activity and superoxide anion production. The results showed that regardless of indoor or outdoor rearing the survival rate of shrimp fed the glucan diet was significantly higher (P<0.001) than that of the control group. The brooders showed enhanced haemocyte phagocytic activity, cell adhesion and superoxide anion production when glucan was administered in their diets. The immunostimulatory enhancement peaked at day 24 after starting the dietary exposure and subsequently decreased to the pre-feeding level at the end of the 40 days feeding trial.

Adjuvants, Immunologic↗

Voltammetric determination of mifepristone at a DNA-modified carbon paste electrode.

A new strategy for the preparation of a DNA-modified carbon paste electrode is developed. It is found that the anodic response of mifepristone is greatly enhanced at the dsDNA-modified carbon paste electrode comparing with that obtained at the bare electrode, while the response at a ssDNA-modified electrode is similar to bare electrode. So the dsDNA-modified electrode is employed as a sensitive biosensor for the detection of mifepristone. A linear dependence of the peak currents on the concentration is observed in the range 2.0 x 10(-7) approximately 2.0 x 10(6) mol/L, with a detection limit of 1.0 x 10(-7) mol/L. The relative standard deviation is 4.3% for six successive determinations of 1.0 x 10(6) mol/L mifepristone. The determination of mifepristone tablets is carried out and satisfactory results are obtained.

Biosensing Techniques↗

A 2-year clinical study of composite and ceramic inlays.

Objective long-term clinical data are necessary to evaluate the performance of dental restorations. This prospective clinical trial evaluated composite and ceramic inlays for clinical acceptability as restorative materials in posterior teeth and provided 2-year results. The study involved 7 student operators placing 47 composite inlays (Tetric, blend-a-lux, Pertac) and 24 ceramic inlays (Empress) under the supervision of an experienced dentist. Clinical assessment of 56 inlays (78.9%) was performed after 2 years with modified USPHS criteria and statistically analyzed using the Mann-Whitney U test and Fisher's exact test. All the ceramic inlays and 90% of the composite inlays were considered clinically excellent or acceptable. During the first year 3 composite inlays failed and during the second evaluation period 1 had to be replaced. Ceramic inlays produced significantly better "anatomic form of the surface" (P = 0.038) and "integrity of the restoration" values (P = 0.043). Inlays in small cavities exhibited superior "marginal integrity" (P = 0.026) and "marginal discoloration" values (P = 0.034). Fisher's test revealed a significantly higher failure rate in molars than in bicuspids (P = 0.034). Posterior tooth-colored inlays exhibited a success rate of 100% for ceramic inlays and 90% for composite inlays even if placed by relatively inexperienced but supervised student operators.

Adult↗

Mechanical properties and wear behavior of light-cured packable composite resins.

OBJECTIVES: Determination of flexural strength, flexural modulus, fracture toughness and wear resistance of three packable composites (Solitaire, Surefil, ALERT) and a packable ormocer (Definite) in comparison with an advanced hybrid composite (Tetric Ceram) and an ion-releasing composite (Ariston pHc). METHODS: Flexural strength, flexural modulus and fracture toughness of each material were determined in three-point bending (each test n = 10). Single-edge notched-bend specimens were used to evaluate the fracture toughness (K1C). Wear of the materials (n = 8) was determined in a pin-on-block-design with a spherical Degusit antagonist at 50 N vertical load and quantified by a replica technique using a 3D-laser scanner. Replicas were made after 6000, 10,000, 30,000 and 50,000 load cycles. The mean wear rate (MWR (micron 3 cycle-1)) was obtained by a linear regression analysis in the steady-state of the time-wear-curve. All results were statistically analyzed with ANOVA and post hoc Tukey HSD tests (p < 0.05). RESULTS: ALERT exhibited the highest flexural modulus (12.5 +/- 2.1 GPa) and K1C (2.3 +/- 0.2 MN m-3/2), but the lowest wear resistance (8275 micron 3 cycle-1). Solitaire presented the highest wear resistance (1591 micron 3 cycle-1), but significantly lower flexural strength (81.6 +/- 10.0 MPa), flexural modulus (4.4 +/- 0.3 GPa), and K1C (1.4 +/- 0.2 MN m-3/2) than all other materials. Surefil revealed a significantly higher flexural modulus (9.3 +/- 0.9 GPa) and wear resistance (3028 micron 3 cycle-1) than Tetric Ceram (6.8 +/- 0.5 GPa; 5417 micron 3 cycle-1) and Ariston pHc (7.3 +/- 0.8 GPa; 7194 micron 3 cycle-1). SIGNIFICANCE: The tested packable composite resins differed significantly in their mechanical properties. This study suggested that fracture and wear behavior of the composite resins are highly influenced by the filler system. Overall, Surefil demonstrated good fracture mechanics parameters and a low wear rate.

Analysis of Variance↗

Fetal ear assessment and prenatal detection of aneuploidy by the quantitative three-dimensional ultrasonography.

The purposes of this study were to use quantitative three-dimensional (3-D) ultrasonography to establish the normal charts of three fetal ear-growth indexes, (ear length, ear width and ear area), and to validate their efficacy in the prenatal diagnosis of fetal trisomies, either separately or as a combination. Using quantitative 3-D ultrasonography, we performed a prospective study to measure the three fetal ear growth indexes (i.e., ear length, ear width and ear area) in 129 singleton pregnancies, including 122 normal fetuses and 7 trisomies. The ear shape was also evaluated in these fetuses using both 2-D and 3-D ultrasonography. Our results showed that: First, 3-D ultrasonography offers better visualization and easier evaluation of fetal ears than 2-D ultrasonography. Second, when using the quantitative assessment of 3-D ultrasonography, the measurements of ear length, ear width and ear area were all correlated significantly with gestational age in normal pregnancies (r = 0.881, 0.848, and 0.890, respectively). In addition, 3 of 7 fetal trisomies had ear length below the tenth percentile, 1 had ear width below the tenth percentile, and 2 had ear area below the tenth percentile. Furthermore, with the combination of these three measurements, (including ear length, ear width and ear area), the sensitivity in detecting fetal trisomies was elevated to 57.1% and the specificity was 83.2%. In conclusion, 3-D ultrasonography reduces the limitations of 2-D ultrasonography in the evaluation of fetal ears. However, fetal ear measurement may not be used as a single ultrasonographic parameter in identifying aneuploid fetuses. We recommend using the combination of these three ear growth indexes to enhance the detection rate of aneuploidy.

Aneuploidy↗

Systemic assessment of fetal hemodynamics by Doppler ultrasound.

The aim of our study was to investigate the parameters of fetal circulation of normal pregnancies and their relationship to fetal cardiac output. We performed a cross-sectional study of 315 normal singleton pregnancies between 20 and 40 weeks' gestation without fetal chromosomal or structural malformations. After follow-up to delivery, 212 patients who fit all the criteria were enrolled for final analysis. Blood flow velocity waveforms were obtained from the tricuspid and mitral ventricular inflow, ascending aorta (AAO), pulmonary artery (PA), middle cerebral artery (MCA), renal artery (RA), umbilical artery (UA), descending aorta (DAO), inferior vena cava (IVC) and ductus venosus (DV) using duplex (real-time Doppler) ultrasound (US) scanner. The peak velocity of DV, AAO, PA and MCA were also obtained. At the intracardiac level, the ratio of peak flow velocity of E wave to peak flow velocity of A wave (E:A ratio) of mitral valve (MV) increased more rapidly than tricuspid valve (TV) E:A ratio. For the great vessels, aortic peak velocity remained higher than the pulmonary peak velocity with advancing gestation. The cardiac output closely correlated to the cardiac compliance and flow resistance indices at arterial and venous level. The acceleration time in the fetal arteries increased with advancing gestation in AAO, PA, MCA and DAO, but it decreased in RA and kept constant in UA. In addition, the acceleration time of UA was unrelated to cardiac output. The changes of the fetal intracardiac, arterial and venous impedances were remarkable through the gestation and related to cardiac output. Fetal cardiac output correlated well with the changes of arterial resistance, except with the DAO. The ventricular compliance increased with advancing gestation, especially in the left side, and was highly related to the change of cardiac output. The acceleration time in major arteries positively correlated with the gestational age and cardiac output, except in UA and RA; this indicates the difference of the changes of mean arterial pressure in uteroplacental circulation, fetal organs and great vessels. In conclusion, the fetal cardiac output correlated well with the ventricular compliance and was influenced by both hemodynamic changes in peripheral resistance and mean arterial pressure.

Adult↗

Assessment of fetal cerebellar volume using three-dimensional ultrasound.

The purposes of this study were to assess the fetal cerebellar volume during normal gestation using three-dimensional (3-D) ultrasound (US) and to establish a normal chart of fetal cerebellar volume using Altman's model of statistics. In total, 231 healthy fetuses were studied for assessment of cerebellar volume using a 3-D US volume scanner. The fetuses to be studied were selected to give a cross-sectional series (i.e., each fetus was examined only once). Polynomial regression analysis was calculated to find the best-fit model using gestational age as the independent variable and cerebellar volume as the dependent variable. Altman's model was used to calculate the age-related reference centiles for the variance of cerebellar volume. In addition, common fetal growth indices, such as biparietal diameter, occipitofrontal diameter, head circumference, abdominal circumference, femur length and estimated fetal weight, were also measured for the correlation between cerebellar volume and these indices. Our results indicated that the fetal cerebellar volume was highly correlated with gestational age in normal pregnancies with the best-fit polynomial regression equation of a second-order (r = 0.91, p < 0.0001). In addition, fetal cerebellar volume in normal gestation is also highly correlated with common fetal growth indices, such as biparietal diameter, occipitofrontal diameter, head circumference, abdominal circumference, femur length and estimated fetal weight (all p < 0.0001). Following the Altman's model, a normal growth chart of fetal cerebellar volume was established for clinical reference. In conclusion, with 3-D US, the assessment of fetal cerebellar volume becomes feasible. We believe that fetal cerebellar volume assessed by 3-D US may be useful in detecting cerebellar hypoplasia and relevant syndromes prenatally.

Cerebellum↗

Three-dimensional ultrasound in the assessment of fetal cerebellar transverse and antero-posterior diameters.

Fetal cerebellum scanning by prenatal ultrasound (US) is very important for early detection of fetal central-nervous-system anomaly, as well as for the determination of gestational age (GA). Due to the small organ size and the unique shape of the fetal cerebellum (CL), accurate measurement of the dimensions of CL by two-dimensional (2-D) US is not easy if the appropriate plane cannot be reached. With the advent of three-dimensional (3-D) US, the disadvantages of 2-D US in assessing the fetal CL dimensions can be avoided. The purpose of this study was to assess the fetal cerebellar transverse diameter (CTD) and cerebellar antero-posterior diameter (CAD) using 3-D US. First, we compared the reproducibility of 2-D and 3-D US on the assessment of fetal cerebellar dimensions. Second, we prospectively measured CTD and CAD in 223 healthy fetuses using a cross-sectional design with an attempt to establish the normal growth charts of fetal CL. Our results showed 3-D US is superior to 2-D US in the reproducibility test of fetal cerebellar dimensions. In addition, with GA as the dependent variable, polynomial regression analysis showed that the best-fit equations for both CTD vs. GA and CAD vs. GA were the first-order. The best-fit predictive equation of GA by CTD was GA (week) = 9.0281 + 0. 58533 x CTD (mm) (r = 0.95, n = 223, SE = 1.82 weeks, p < 0.0001), and the best-fit predictive equation of GA by CAD was GA (week) = 10. 855 + 1.1672 x CAD (mm) (r = 0.82, n = 223, SE = 3.41 weeks, p < 0. 0001). Furthermore, all the correlation coefficients of CTD or CAD vs. the common fetal growth indexes were also highly significant (all p < 0.0001). In conclusion, our data of fetal CL dimensions assessed by 3-D US may serve as a useful reference in assessing fetal CL growth, dating GA or detecting fetal CL anomalies.

Anthropometry↗

Role of the melanocortin-4 receptor in metabolic rate and food intake in mice.

We evaluated the role of the melanocortin-4 receptor (MC-4R) in the control of metabolic rate and food intake in mice. Intraperitoneal administration of the non-selective MC-R agonist melanotan II (MT-II; a cyclic heptapeptide) increases metabolic rate in wildtype mice, while MC-4R knockout mice are insensitive to the effects of MT-II on metabolic rate. MC-4R knockout mice are also insensitive to the effects of MT-II on reducing food intake. We conclude that MC-4R can mediate control of both metabolic rate and food intake in mice. We infer that a role for MC-3R in mediating the acute effects of MT-II on basal metabolic rate and food intake in wildtype mice seems limited.

Animals↗

Esophageal varices in congenital heart disease with total anomalous pulmonary venous connection.

Total anomalous pulmonary venous connection (TAPVC) is an uncommon congenital anomaly in which the anatomical presentations vary widely among patients. We hereby present two newborns with TAPVC associated with asplenia syndrome; both had severe esophageal varices due to infradiaphragmatic pulmonary venous drainage. Ultrafast computed tomography (CT) scanning was superior to color Doppler echocardiography and cardiac catheterization as it provided a detailed portrait of the pulmonary drainage. The remarkable radiographic manifestations are presented.

Cardiac Catheterization↗