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Biomedical subjects

H Xu

Publications and source records attributed to H Xu.

At least 379 records · Page 21Linked to original sources
PubMed1999

[Experimental study on multi-infarct dementia treated with reinforcing essence to refresh mental activity method].

OBJECTIVE: To explore the mechanism of multi-infarct dementia (MID) treated with reinforcing essence to refresh mental activity method (RERM). METHODS: MID rat models were established by injecting sterile dry blood clots into common carotid artery and screening by the first jumping-off latency of diving platform reflex. Effect of RERM on model rats in learning, memory, serum and brain malondialdehyde (MDA), superoxide dismutase (SOD) level, brain monoamine neurotransmitter content, and brain morphosis were observed. RESULTS: Obvious malfunction of learning and memory was found in MID rat models, and there were also significant decreasing of monoamine neurotransmitters content in partial brain zones, decreasing of SOD activity in brain and increasing of MDA content in serum and brain. RERM could obviously improve learning and memory, raise SOD activity and monoamine neurotransmitters content in brain tissue, lower MDA content in serum and brain of MID rat models, protect brain morphosis of multi-infarction rats. CONCLUSION: RERM might treat MID by restraining lipid peroxidation, improving monoamine neurotransmitters content in partial brain zones and decreasing ischemic damage of brain tissue.

Animals↗
PubMed1999

[Protective effects of bactericidal/permeability-increasing protein on the vital organ function after endotoxic shock in rats].

OBJECTIVE: To investigate the protective effects of BPI on the vital organ function after endotoxic shock in rats, and to provide experimental evidence for further clinical studies. METHODS: 12.5 mg/kg of E. coli 026:B6 LPS were bolus injected via i.a. to reproduce endotoxic shock. Immediately after injection of LPS, 5 mg/kg of BPI or equal volume of physiological saline were bolus injected via i.a. RESULTS: The survival time of shocked animals was prolonged in the BPI group, 24 h survival rate was higher than that in the saline group. The values of mean arterial pressure, left intraventricular systolic pressure, isovolemic ventricular pressure, and dp/dtmax in the BPI group were markedly higher than those in the saline group. The plasma glutamic-pyruvic transaminase and urea nitrogen levels were obviously lower than those in the saline group. The plasma endotoxin and TNF levels in the BPI group were significantly lower than those in the saline group. CONCLUSIONS: BPI has good protective effects on cardiac, hepatic, and renal function in endotoxic shock in rats, indicating that BPI might be useful in clinic treatment of sepsis/septic shock.

Animals↗
PubMed1999

[Biologic behavior and prognosis of patients with gastric cancer in young persons].

OBJECTIVE: To explore the relationship between the biological behavior and prognostic factors in treating gastric cancer of young persons by various surgical methods. METHODS: 89 young patients with gastric cancer aged 35 years or less were analyzed. RESULTS: The resectable rate was 73.0% (65/89), radical resectable rate 48.3% (43/89), and palliative resectable rate 24.7% (22/89). The 5-year survival rate was 24.7% (22/89) and 48.8% (21/43) for the radical group. Biological characteristics included infiltrated, diffused growth, lymph node metastasis, female predominance. CONCLUSIONS: The biological behavior of gastric cancer in young persons is poor. Early management gastric cancer and radical operation combined with complex treatment are essential.

Adult↗
PubMed1999

[An experimental study on effect of methazolamide on decreasing intraocular pressure in rabbits].

OBJECTIVE: To study the therapeutic effect of methazolamide on glaucoma. METHODS: Various doses of methazolamide were orally administered in rabbits, their effects on high intraocular pressures and on recovery of low intraocular pressures were observed. RESULTS: The high intraocular pressures were significantly decreased and the maintenance of low intraocular pressure was significantly prolonged by the oral administration of 40, 20, 10 mg/kg of methazolamide. A dose-effect relationship that is the reduction of intraocular pressure increases with the increase of the dosage administration of methazolamide could be observed. CONCLUSION: This study indicates that methazolamide might play an effective therapeutic role in glaucoma.

Animals↗
PubMed1999

[Retreatment after excimer laser photorefractive keratectomy].

OBJECTIVE: To evaluate the efficacy and safety of reoperation of residual myopia because of regression, undercorrection with or without coexisting severe corneal haze after excimer laser photorefractive keratectomy (PRK). METHODS: A VISK 20/20 excimer laser was used to treat myopia of -1.00 to -16.50 diopters (spherical equivalent) at corneal plane. 35 patients (51 eyes) who had residual myopia because of regression, undercorrection with or without coexisting severe corneal haze after initial PRK were retreated by using PRK and phototherapeutic keratectomy. RESULTS: At 12 months after retreatment, the rates of uncorrected visual acuity more than or equal to 10/20 or 20/20 were 82.6% and 39.1% respectively, 100.0% and 50.0% in myopia up to -6.00 D, 73.3% and 33.3% in myopia of -6.25 to -16.50 D respectively. 86.3% of eyes were within +/- 1.00 diopters of desired emmetropia. Corneal haze after retreatment was not greater than that observed after initial procedures. CONCLUSIONS: The residual myopia because of regression, undercorrection with or without severe corneal haze after initial PRK can be successfully retreated. Although the therapeutic result of retreatment of PRK is less successful than that of the initial procedure, retreatment appears to enhance it.

Adult↗
PubMed1999

[Association of body size at birth with impaired glucose tolerance during their adulthood for men and women aged 41 to 47 years in Beijing of China].

OBJECTIVE: To explore if there was relationship between small body size at birth and type 2 diabetes and impaired glucose tolerance (IGT) during adulthood in China. METHODS: Six hundred and twenty-eight singletons born in the Peking Union Medical College Hospital in Beijing during July 1948 to the end of 1954 were followed-up and their medical records at birth were abstracted. Anthropometry and standard oral glucose tolerance test were carried out for all of them. Plasma insulin level was measured with radioimmunoassay for them. RESULTS: Prevalence of type 2 diabetes and IGT decreased with the increase in their birth weight, Ponderal index (PI) and head circumference at birth (for trend test, chi(2) = 6.7, P = 0.01; chi(2) = 4.8, P = 0.03; and chi(2) = 5.8, P = 0.02; respectively), with the highest of 43.8% in those with thin body size (PI < 24 kg/m(3)) at birth and obesity (BMI < 75 percentile) during adulthood, and the lowest of 8.3% in those with more fat at birth (PI >or= 28 kg/m(3)) and keeping relatively thin (BMI < 25th percentile) during adulthood. BMI of mothers in their early and late pregnancy correlated reversely with blood glucose levels two hours after sugar load for their children in adulthood. In addition, body weight, PI and head circumference at birth correlated reversely with their plasma insulin levels fasting and two hours after sugar load and levels of 32 -- 33 split pro-insulin during their adulthood. After adjustment for current BMI, lifestyle and economic status, body size at birth mentioned above still associated with their glucose tolerance and level of plasma insulin during adulthood. CONCLUSION: There was relationship between small body size at birth and prevalence of type 2 diabetes and IGT during adulthood. Prevalence of type 2 diabetes and IGT depended on the synergic effect of thin body size at birth and obesity during adulthood.

Adult↗
PubMed1999

[Experimental study on ultrashort wave therapy on the healing of fracture].

The models of fracture in the rabbit radii were made. The rabbits in the experimental group were treated--with ultrashort wave at the fracture sites. The changes in the x-ray, histology, and biomechanics were observed dynamically. The results were that the speed and quality of the fracture healing in the experimental group were much better than in the control group.

Animals↗
PubMed1999

[Investigation of the bone mineral density in hemodialysis patients for different terms].

The investigate the change of bone mineral density(BMD) in hemodialysis patients for different terms of hemodialysis, 50 patients were divided into three groups according to the duration of hemodialysis. Group I was < 5 years. Group II was 5-10 years, and Group III > 10 years. The BMD of the patients were assayed by using dual energy X-ray bone absorptiometer. Serum calcium, phosphorus, aluminium, and intact parathyroid hormone(iPTH) were detected at the same time. The mean BMD and serum iPTH were 0.624 +/- 0.180 g.cm-2 and 9.67 +/- 9.46 ng.cm-1 in Group I, 0.573 +/- 0.160 g.ml-2 and 10.16 +/- 28.05 ng.ml-1 in Group II, and 0.513 +/- 0.117 g.cm-2 and 10.75 +/- 24.94 ng.ml-1 in Group III, respectively. There was no statistical significance in BMD (P > 0.05) and iPTH(P > 0.05) among the above groups. The BMD of hemodialysis patients is not affected by different terms of hemodialysis.

Bone Density↗
PubMed1999

[Effect of gui xin tong on sorbitol and nerve conduction velocity in diabetic rats].

OBJECTIVE: To investigate the effect of Gui Xin Tong(GXT) on sorbitol and nerve conduction velocity in diabetic rats. METHOD: The model rats suffering from diabetes were induced by STZ, and blood sugar levels above 16 mmol/L were chosen for observation. The rats were divided into GXT group, AG group and control group. The nerve conduction velocity, aldose reductase activity and sorbitol in STZ induced diabetic rats were investigated. RESULT: Compared with the control group GXT could increase the sciatic nerve conduction velocity significantly (P < 0.05), decrease the RBC and sciatic nerve, aldose reductase activity, and RBC-sorbitol content. CONCLUSION: GXT has the effect of improving nerve conduction velocity significantly and reducing aldose reductase activity and sorbitol

Aldehyde Reductase↗
PubMed1999

[Studies on quality standard of Fructus Amomi].

OBJECTIVE: To study the quality standard of different parts and species of Fructus Amomi from different producing. METHOD: GC method, ash determination and water determination. RESULT: The contents of bornyl acetate in different parts and species of Fructus Amomi from different producing areas were obviously different, but the traditional curative effects remained unchanged. The standards for the total ash content, acid-insoluble ash content and water content in Fructus Amomi have been set up. CONCLUSION: Bornyl acetate with a definite content limit should be chosen as the index in studying the quality standard of Fructus Amomi.

Amomum↗
PubMed1999

[Study of cleft palate with problems of middle ears and its treatment].

OBJECTIVE: To gain a clear idea of the following: 1. the relationship between the incidence of functional obstruction of middle ear and surgery on cleft palate and patients' age; 2. the bacterial culture of secretion taken from the tympanic cavity with secretory otitis media; 3. the preliminary effects of treatment by inserting a tube into the tympanic cavity of a patient with secretory otitis media. METHODS: 275 middle ears of patients with cleft palate were selected, then acoustic immitence examination and hearing threshold of pure tone were adopted, and bacterial culture was carried out for studying secretion of the middle ear with secretory otitis media. RESULTS: The tympanograms of 75.5% ears of the patients before cleft palate operation were found to be abnormal, among which patients under 3 years were 81%, and 41.2% ears of those after closure of cleft palate were abnormal. Gram's positive and negative germs of bacterial culture were 20.1% and 24% respectively. The hearing of 6 ears with secretory otitis media has recovered from the moderate hearing loss due to tube insertion. And the tympanograms of 4/8 ears were transformed from type B to type A. CONCLUSION: It is beneficial to improve function of Eustachian tube if cleft palate can be repaired at an early stage and beneficial to improve the patients' hearing and devlope their speech if a tube is inserted into the typanic cavity in cases of secretory otitis media at an early stage.

Adolescent↗
PubMed1999

[p53 gene expression of human hepatoma cell lines and their sensitivities to parvovirus H-1].

DNA structure and expression of p53 gene in human hepatoma cell lines SMMC-7721, YY-8103 and a spontaneously transformed liver cell line L-02 were analysed using the following method: analysis of allelic losses on chromosome 17p, PCR/SSCP, Northern blot and immunoprecipitation. There was no point mutation found in the exons 4-9 of the p53 gene, and a low level of expression of p53 gene was detected in the three cell lines. These observations were in agreement to the reported results of the relevant experiment using the human hepatoma cell line QGY-7703. Sensitivities of these cell lines and other eight human hepatoma cell lines (QGY-7703, PLC/PRF/5, Tong/HCC, Huh-7, FOCUS, Hep3B, SK-Hep-1, HepG2) with known p53 backgrounds to parvovirus H-1 was assayed using MTT method. Abnormality in the structure and/or function was observed in all of the cell lines examined except HepG2. The cell line HepG2 with normal structure and function of the p53 gene was found to be the least sensitive to H-1 in comparison to all the cell lines which have defeated structure and/or function of the p53 gene. The present study serves as a preliminary evidence that enhancement of the sensitivity of human hepatoma cell lines to H-1 is correlated to the abnormality of the structure and/or function of the p53 gene.

Carcinoma, Hepatocellular↗
PubMed1999

[Antibiotic resistance and plasmid profiles of Vibrio isolates from cultured Sparus sarba].

A total of 51 potential pathogenic vibrios were isolated from moribund silver seabream Sparus sarba, which were collected from local fish farms of Hong Kong. All the isolates were classified and identified as 7 species by the API 20 E system and the scheme of Alsina & Blanch. These species were Vibrio alginolyticus (24 strains), Vibrio vulnificus (12 strains), Vibrio parahaemolyticus(7 strains), Vibrio logei(4 strains), Vibrio pelagius II(2 strains), Vibrio fluvialis (1 strains) and Vibrio meditterranei (1 strains). Among these isolates, the three predominant species (V. alginolyticus, V. vulnificus and V. parahaemolyticus) were confirmed to be virulent to sea bream by experimental challenge. All isolates were also screened for plasmid DNA by agarose gel electrophoresis and tested for susceptibility to 16 antimicrobial agents by the agar dilution method. Of the 51 isolates examined, all strains were sensitive to ceftriaxone, streptomycin, nalidixic acid and rifampicin, and almost all were sensitive to ceftazidime, netilimicin, chloramphenicol and sulfamethoxazole except one or two strains. Most isolates were resistant to ampicillin (60. 8%), cefuroxime (66.7%), amikacin(55%), kanamycin(58.8%) and trimethoprinm (76.5%). Fifteen of the 51 isolates harboured 1-4 plasmids, with sizes ranging from 9 to 123 kb. Both the plasmids and the associated antimicrobial resistance (ampicillin, cefuroxime and trimethoprim) of 9 isolates could be transferred to recipient by single-step conjugation, however, the frequencies were very low, ranging from 10(-11) to 10(-9). The present results indicate that resistance to these antibiotics is chromosomal.

Animals↗
PubMed1999

[Studies on preparation of L-phenylalanine from phenylpyruvic acid by E. coli EP8-10].

E. coli EP8-10 was selected from the soil. It was able to produce the transaminase with high activity when it was cultivated on the medium containing peptone and beef extract. Optimum conditions of enzyme reaction was: phenylpyruvic acid's concentration of 0.3-0.5 mol/L, L-Asptaric acid used as amino donor, pH 8.5 37 degrees C. When phenylpyruvic acid was 0.3 mol/L, 48 g/L L-phenylalanine was produced after 6 h with 97% conversion rate.

Culture Media↗
PubMed1999

[Determination of T lymphocyte subsets in peripheral blood lymphocytes of patients with chronic tonsillitis].

OBJECTIVE: To observe the cellular immunologic status of patients with chronic tonsillis. METHOD: T lymphocyte subsets in peripheral blood lymphocytes of 60 cases with chronic tonsillis were detected. RESULT: It was found that CD4, CD4/CD8 in chronic tonsillis patients decreased than that in normal control group with statistical significance (P < 0.01). CONCLUSION: This study indicates that the cellular immunologic status in patients with chronic tonsillitis was decreased.

Adolescent↗
PubMed1999

[C677T genetic polymorphism of methylenetetrahydrofolate reductase in premature coronary heart disease].

OBJECTIVE: Methylenetetrahydrofolate reductase (MTHFR) is an important factor responsible for hyperhomocysteinemia. The relation of MTHFR gene C677T polymorphism and premature coronary heart disease was studied. METHODS: MTHFR C677T genetic polymorphisms in 67 patients with premature coronary heart disease were detected by PCR-RFLP technique. RESULTS: In case group, the frequency of T homogenic type was 34.3% (23/67), heterogenic type 43.3% (29/67) and C homogenic type 22.4% (15/67). T allele frequency was 55.9% (75/134) while C allele frequency 44.1% (59/134) in case group. There were significant differences in MTHFR genotype and allele frequencies between cases and controls (chi 2 = 6.82 and 5.41 respectively, P < 0.05). CONCLUSIONS: It was suggested that MTHFR gene C677T mutation was a possible risk factor of Chinese premature coronary heart disease.

Adult↗