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H Wysocki

Publications and source records attributed to H Wysocki.

106 records · Page 6Linked to original sources
PubMedDate not supplied

The effect of selected antiarrhythmic drugs on neutrophil free oxygen radicals production measured by chemiluminescence.

The evidence that free oxygen radicals produced by polymorphonuclear neutrophils (PMN) participate in generation of reperfusion arrhythmias is well documented. The in vitro effect of selected antiarrhythmic drugs on PMN free radicals production was evaluated by luminol- (LuCL) and lucigenin- (LgCL) amplified chemiluminescence stimulated with opsonized zymosan (o.z.) and phorbol myristate acetate (PMA). Fast sodium channel inhibitors varied in the influence on PMN chemiluminescence: from an inhibition in all models studied by procainamide, to lack of an effect by mexiletine. Propafenone, similarly to ajmaline and verapamil, inhibited LuCL stimulated with PMA, as well as LgCL after stimulation with both PMA and o.z. Bretylium tosylate decreased LuCL stimulated with both inducers, with no effect on LgCL. Amiodarone in high concentrations inhibited both LuCL and LgCL. beta-blockers propranolol and practolol impaired LuCL stimulated with o.z., as well as LgCL induced with PMA, whereas alpha-blocker phentolamine inhibited LuCL and LgCL stimulated with both inducers. The drugs' effect on PMN free oxygen radicals production may constitute an additional mechanism of their activity.

Acridines↗
PubMedDate not supplied

The influence of increasing glucose concentrations on selected functions of polymorphonuclear neutrophils.

The influence of increasing glucose concentrations on some essential functions in polymorphonuclear neutrophils (PMN) was evaluated in vitro in 20 healthy persons. PMN chemotaxis, adherence, phagocytosis and bactericidal capacity were estimated. Higher than physiologic glucose concentrations were found to diminish chemotaxis, phagocytosis and bactericidal capacity. The results obtained may indicate another possible mechanism that accounts for the increased susceptibility to infection observed in diabetic patients. However, PMN adherence rose parallel with increasing glucose concentrations reaching maximum values at 300 mg/dl (16.6 mmol/l). The changes of PMN adherence may suggest the participation of these cells in the development of microangiopathic injuries.

Adult↗
PubMedDate not supplied

Polymorphonuclear neutrophils adherence, superoxide anion (O2-) production and HBA1 level in diabetic patients.

It was established that metabolic disturbances accompanying the impairment of diabetes control induced polymorphonuclear neutrophil (PMN) activation. Therefore, we evaluated the influence of diabetes control, measured as HbA1, level on superoxide anions production and neutrophils adherence to foreign surface. Twenty patients with insulin-dependent or non-insulin-dependent diabetes being in different state of metabolic control were studied. It was revealed that PMN from diabetics demonstrated significantly increased unstimulated superoxide anion production (11.07 +/- 1.25 vs. 4.15 +/- 1.24 nmol O2-/10(6) PMN/10 min). Stimulated O2- production did not reach the same levels as controls (21.80 +/- 1.34 vs. 23.67 +/- 4.36 nmol O2-/10(6) PMN/10 min). There were highly significant correlations between unstimulated O2- production and HbA1, levels (r = 0.86). Adherence of diabetic PMN suspended in autologous plasma was significantly increased in comparison to controls (12.76 +/- 1.20 vs. 6.04 +/- 1.99 cells/mm2). However, these data did not correlate with HbA1 level (r = 0.06). In conclusion our results seems to indicate that metabolic control of diabetes influence PMN function.

Adult↗
PubMedDate not supplied

Effects of magnetic resonance imaging on polymorphonuclear neutrophil adhesion.

BACKGROUND: Limited research has been performed concerning the effects of MR imaging on the immune system. In this study the influence of MR imaging exposure on polymorphonuclear neutrophil (PMN) adhesion was evaluated. MATERIAL AND METHODS: In vivo and in vitro studies were performed in 10 patients undergoing an MR imaging procedure, PMN adhesion to a plastic surface, as well as the expression of adhesion molecules beta 2-integrins CD11b, CD18, and L-selectin on the surface of PMN were estimated. RESULTS: Exposure to MR imaging significantly increased adhesion of isolated PMNs to plastic surfaces. PMNs from blood samples obtained from patients undergoing MR imaging as well as from blood samples placed beside patients during MR imaging did not differ from controls in adhesion to plastic surfaces. Similarly, plasma from three tested samples did not change control PMN adhesion to plastic surface. Expression of beta 2-integrins (CD11, CD18) was significantly increased in samples left beside patients during MR imaging, while significantly decreased in samples obtained from patients after MR imaging exposure when compared to control samples. Expression of the surface adhesion molecule L-selectin on the surface of PMN decreased significantly in blood samples left beside patients during MR imaging. CONCLUSION: The results indicate that the PMN adhesion properties increase under the influence of MR imaging exposure. This phenomenon may be the result of direct stimulation of polymorphonuclear neutrophils by the exposure to MR imaging.

Adult↗
PubMedDate not supplied

[Superoxide anion production and adhesiveness of neutrophils in obese patients].

In 15 subjects with simple obesity and 10 patients with obesity associated with type II diabetes neutrophil adhesiveness and the rate of resting and stimulated production of superoxide anions (O2-) by these cells were assessed. High values were demonstrated of neutrophil adhesiveness suspended in autologous plasma in both groups of patients. The value of O2- production by resting cells was significantly raised, particularly in cases of simple obesity. Stimulated production of superoxides by neutrophils was approaching the value noted in the control group. The obtained results may suggest participation of these cells in the development of atherosclerotic changes.

Adhesiveness↗
PubMedDate not supplied

[Lysozyme activity (LZM) and unsaturated vitamin B-12-binding capacity (NZW vit B-12) in the serum following prednisone administration in patients with bone marrow aplasia with pancotypenia].

In 18 patients with bone marrow aplasia with pancytopenia lysozyme activity and unsaturated vitamin B12-binding capacity in the serum were determined. These investigations, together with determinations of peripheral blood polymorphonuclear count, were done again after prednisone administration. In all cases a significant fall was found in the NZW vit B12 and LZM activity in the serum. A slight rise in the polymorphonuclear count in the 24th hour of the study was associated with a rise in the NZW wit. B12 in the serum, and decreased LZM activity. This confirmed the previously demonstrated complex character of corticosteroid action on the system of polymorphonuclears. These results point also to the usefulness of determination of unsaturated vitamin B12-binding capacity for evaluating the value of the total granulocyte pool in granulocytopenia.

Adult↗
PubMedDate not supplied

Evaluation of the effect of oral enalapril on neutrophil functions: comparison with the in vitro effect of enalapril and enalaprilat.

Polymorphonuclear neutrophils (PMN) participate in the development of myocardial injury by releasing free oxygen radicals and by involvement in the no-reflow phenomenon. Neutrophil-mediated myocardial injury, therefore contributes to the pathogenesis of heart failure. We investigated the effect of oral treatment with enalapril on neutrophil free oxygen radical production, aggregation and adherence in patients with moderate heart failure (New York Heart Association-NYHA II and III degrees). Samples were taken before and 48 h after a single 10 mg oral dose. Oral enalapril inhibited hydrogen peroxide released by unstimulated PMN, but did not affect stimulated H2O2 release, superoxide anion production, adhesion or aggregation of PMN. Enalaprilat in vitro stimulated PMN to release H2O2 and superoxide anions. Furthermore, in the in vitro conditions both enalaprilat and enalapril inhibited hydrogen peroxide release by stimulated cells. We conclude that, despite certain modifications of neutrophil function in vitro, oral administration of enalapril seems to exert a limited biological effect on circulating neutrophils.

Adult↗
PubMedDate not supplied

[Usefulness of hydrocortisone test for assessment of bone marrow reserve in neutrophils].

The activity of lysozyme and unsaturated binding capacity of vitamin B12 in the serum were determined in hydrocortisone test for calculating the reserve of granulocytes in bone marrow. A significant rise in the count of neutrophils in peripheral blood 3 hours after the beginning of the test was not associated with statistically significant changes in the determined parameters. This in an evidence that prolongation of the survival of cells in the circulation as one of the mechanisms of corticosteriod action on the granulocyte system is without any greater importance. This is connected, most probably, with short duration of the test. The rise of neutrophil count in peripheral blood after hydrocortisone administration may be regarded, therefore, as a measure of the value of one marrow reserve of these cells.

Bone Marrow↗