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H Wurm

Publications and source records attributed to H Wurm.

At least 37 records · Page 2Linked to original sources
PubMed1987

Free and Apo B-associated Lpa-specific protein in human serum.

The distribution of Lpa-specific protein (Apo A) among various fractions of human serum was investigated. The following results were obtained. In very low density lipoproteins (VLDL) of fasting human serum Apo A cannot be detected. Most of Apo A is complexed to LDL forming lipoprotein a (Lpa) present in the density fraction of 1.006-1.125 g/ml. Only approximately 5% of Apo A are found in the d greater than 1.125 bottom fraction after ultracentrifugation. Phosphotungstate-Mg (PTA), heparin-Mn (Hep) and dextran sulfate-Mg (DS) precipitate approximately 95% of serum Lpa together with the Apo B containing lipoproteins. Also monospecific antibodies against Apo B do not precipitate all Lpa and leave approximately 3% of Apo A in the supernatant. With polyethylene glycol (PEG) on the other hand, virtually all Lpa is removed since this reagent also precipitates Lpa and Apo A. Apo A of the bottom fraction after ultracentrifugation and of the supernatant after selective precipitation is not stainable with Sudan black and probably represents a protein which is not associated with lipids.

Adolescent↗
PubMed1987

Beta 2-glycoprotein-I (apo-H) inhibits the release reaction of human platelets during ADP-induced aggregation.

In order to further characterize the modulation of the ADP-induced aggregation of gel-filtered human platelets by beta 2-glycoprotein-I (beta 2-G-I), the influence of this glycoprotein upon the serotonin (5-HT) release during aggregation was measured. The following results were obtained: beta 2-G-I completely inhibits the 5-HT release during ADP-induced platelet activation. The inhibition is correlated with the inhibition of the second wave of the ADP-induced aggregation. This effect of beta 2-G-I is not dose-dependent and appears above a threshold concentration of 0.1-0.15 mg/ml in the assay. The specificity of the beta 2-G-I interaction with the ADP-activation is supported by results obtained with collagen or thrombin as aggregating agents. In these cases neither the aggregation nor the release is influenced by the glycoprotein. In respect to the results obtained, beta 2-G-I is a potent candidate to be a modulator of ADP-induced platelet activation in vivo.

Adenosine Diphosphate↗
PubMed1987

Cyclooxygenase and lipoxygenase metabolites during platelet aggregation: quantitative measurement by negative ion chemical ionization--gas chromatography/mass spectrometry.

This study was aimed at investigating systematically the aggregation of human gel filtered platelets induced by various physiological stimuli such as thrombin (0.25 U/ml), collagen (2 micrograms/ml), a mixture of thrombin and collagen, and ADP (2-5 microM). For quantitative measurement of TXB2, PGF2 alpha and 12-HETE, negative ion chemical ionization-gas chromatography/mass spectrometry using stable isotope dilution was applied. Stimulation by thrombin, collagen and the combined agonists resulted in an increase of TXB2 (up to 54 ng/1 X 10(8) platelets) and 12-HETE (up to 44 ng/1 X 10(8) platelets) during platelet aggregation. The stimulation with ADP showed only little increase of these metabolites and this only during the second wave of aggregation. Very little amounts of PGF2 alpha were produced during thrombin stimulated aggregation.

12-Hydroxy-5,8,10,14-eicosatetraenoic Acid↗
PubMed1986

Prothrombinase activity of human platelets is inhibited by beta 2-glycoprotein-I.

In the present paper the influence of beta 2-glycoprotein-I, also known as apolipoprotein H, upon the prothrombinase activity of platelets and phospholipid vesicles was investigated. The results can be summarized as follows. 1. The prothrombinase activity of resting, non-activated platelets, lysed platelets and vesicles composed of phosphatidylserine and phosphatidylcholine at different molar ratios is inhibited by beta 2-glycoprotein-I in a dose-dependent manner. The concentration of glycoprotein which produces marked inhibition is within the physiological plasma concentration range of beta 2-glycoprotein-I. 2. The time dependence of this inhibition is a relatively slow process, which is not fully expressed before 1 h of incubation. 3. The effect of the glycoprotein is not due to a direct interaction with the components of the prothrombinase complex, i.e. factors Xa, Va, Ca2+ or prothrombin, nor is the inhibitory action abolished by increasing concentrations of coagulation factors Xa and Va. This suggests that beta 2-glycoprotein-I causes a reduction of the prothrombinase binding sites of these coagulation factors to platelets or phospholipid vesicles. 4. The prothrombinase activity of platelets stimulated with ionophore A23187 or with collagen plus thrombin is also inhibited by beta 2-glycoprotein-I in a manner similar to that observed for phospholipid vesicles or for lysed platelets. These findings suggest a regulatory role for beta 2-glycoprotein-I in the pathway of blood coagulation.

Blood Platelets↗
PubMed1986

[History of methods for estimating body height by skeletal findings. Recommendations for estimating body height by skeletal findings up to the middle of the 20th century].

Up to now no historical review concerning the methods to estimate body height from skeletons has been given. As well no collection of the formulae relevant to this subject, which would save laborious search in the wide-scattered literature, is available hitherto. This study tries to fill this gap. The anthropological and statistical treatment of the populations under study will not be discussed in this connection, because this has to be left to a separate study.

Anthropology, Physical↗
PubMed1986

[Constitution and nutrition. III: On the constitution and nutrition of the Germans in early history].

This documentation is part of an inquiry about constitution and nutrition in Germany since early history. In this documentation, authorities of antiquity and modern researches about constitution and nutrition in early Teutonic history were collected and then possible influences of nutrition of that period on constitution of early Teutons were analysed. The early Teutons were characterised by great and strong stature, tendence to dolichocephalie and were of spectacular vigour. Their food was characterised by vitality and growth stimulating ingredients (like milk, meat, barley, oats, millet), but with differences in quantity in course of the year. A comparisation between nutrition and constitution of early Teutons shows a connection among typical patterns of early-Teuton-constitution and these nutrition. Differences in constitution to the southern neighbours, Celts and Romans, were not so marked, that constitutional changes in Germany, especially in southern Germany, in the middle ages can be explained by mixed race.

Body Constitution↗
PubMed1986

[Specific selection of recommendations for estimating body height from skeletal findings, comparability of estimated results and general problems in estimating body height during life].

Problems in estimating stature from skeletal remains are not enough analysed in anthropology. Certainly, a great number of statements exists but the problems of recommendability and comparability of these statements must studied more complete. Reasons for these ommisions must be searched in the neglect of the fact that physical constitutions differed in historical and geographical respects. Therefore in this paper, some basic problems in estimating stature are represented, some aids for better comparability and recommendability are given.

Anthropology, Physical↗
PubMed1985

Influence of beta 2-glycoprotein-I upon the content of cAMP and cGMP in human blood platelets.

The influence of beta 2-glycoprotein-I on the cyclic nucleotide content in human gel filtered platelets (GFP) was studied: beta 2-G-I up to a concentration of 5 mg/ml does not alter the basal cAMP level in resting GFP and also does not influence the action of PGE1 which strongly inhibits platelet aggregation by elevation of cAMP. Collagen induced aggregation of GFP leads to an increase of the platelet cGMP content. Preincubation of GFP with 0.2 mg/ml of beta 2-G-I enhances significantly the cGMP increase by collagen. In a similar manner beta 2-G-I also amplifies the increase in cGMP concentration induced by ADP. In this case, a concomitant alteration of the aggregation pattern is observed. Thrombin produces a much higher cGMP elevation in GFP than ADP or collagen. The admixture of 0.2 mg/ml of beta 2-G-I is not able to enhance further the thrombin effect on cGMP. The results are discussed in view of the possible significance of these observations for platelet aggregation in vivo.

Adenosine Diphosphate↗
PubMed1985

Interaction of beta 2-glycoprotein-I with human blood platelets: influence upon the ADP-induced aggregation.

The interaction of beta 2-glycoprotein-I (beta 2-G-I), a plasma constituent of unknown function, with blood platelets was studied. The following results were obtained: 1) beta 2-G-I binds to washed human platelets isolated by centrifugation (WP) at one kind of specific, saturable binding sites. The dissociation constant was found to be approx. 1 X 10(-6) M. In the presence of physiological concentrations of Ca++ (2.5 mM), this specific binding is markedly reduced. Unspecific binding of beta 2-G-I to platelets, however, is not influenced by Ca++. Platelets prepared by gel filtration (GFP), differing in their in vitro aggregability from WP, exhibit no specific binding of beta 2-G-I. Binding to GFP is also not induced by activation with thrombin, collagen or ADP. beta 2-G-I causes significant alteration of the ADP-induced aggregation of GFP. Aggregation induced by thrombin, collagen, arachidonic acid or PAF-acether, however is not altered by beta 2-G-I. It is suggested, that pelleting during centrifugation causes irreversible rearrangements in the membrane of platelets.

Adenosine Diphosphate↗
PubMed1985

[Not Available].

Explore the source record for details and available documents.

Diet↗
PubMed1985

[Average body height of the middle and lower social classes in the middle European Germanic settlement area from the early Middle Ages to modern times].

This paper reviews the body height data of middle and low social classes of North and Central European populations from the early Middle Ages until the beginning of the 17th century. It is mainly based on anthropological data from the ancient Germans, but considers also some historical reports. From the data presented here it is seen that the initially rather considerable body heights in these two social groups are decreasing during the Middle Ages. This could be observed on population samples from the Central German Highlands and from Southern Germany as well as on samples from the utmost Western Teutonic settlement: Greenland. The reasons for these variations are most likely to be seen in nutritional changes, in increasing hard physical work due to the transition to predominating agriculture, in biological effects of birthrights, and in deteriorating hygienic conditions.

Anthropology, Physical↗
PubMed1985

[History of the determination body height from skeletal findings (body height determination for men). The proposed methods of body height determination from skeletal findings since the middle of the 20th century].

A scientific historical review of the procedures of stature estimation according to skeleton finds including a collection of important tables and formulae replacing the troublesome looking for formulae and tables in the scattered publications was still missing. In the 1st part, a collection of estimations published since the middle of the 18th century to the middle of the 20th century is therefore presented. In this part, a collection important procedures of stature estimation published since the middle of our century is given. The presentation follows the chronological succession. A short description of all mentioned base populations is included in the text. A thorough critical anthropological and statistical analysis of base populations and estimations was not intended, because this should be the topic of a separate research.

Age Factors↗
PubMed1985

[Hypotheses and causes of body height progressions since mid 19th century--a scientific historical review. I: Discussion of causes since the end of the 19th century to mid 20th century, including references on body height development in the 19th century].

This examination is trying to give a scientific historical survey of the hypothesis and the reasons for progression of height since the middle of the 19th century. These progressions of height are not based on a single reason, but were kept going by the addition of consecutive reasons. Physical relief is mainly responsible for the most recent phase of progression of height.

Adolescent↗
PubMed1985

[Hypotheses and reasons for the progression of body height since the middle of the 19th century--a science history retrospect. II. A discussion of the reasons since the middle of the 20th century including some terminological considerations].

In part I of this paper, reasons for secular height-progression till the middle of our century were discussed. In the beginning, reasons were seen in the improvement of nutrition and in lightening from strong physical load. To the middle of our century, more and more hypothetical reasons were alleged. After this time, science found back to new reality. The secular height-progression is the consequence of nutritive improvements, especially of increased consumption of animal protein. An chief matter seems to be the nutrition of babies with food containing more protein than by breast-fed childs. Early child-nutrition may to be like a stamping for endocrine activity. The last phase of stature's increase is caused by intensive lightening from physical load in the course of modern mechanization, which advanced bone-growth of the legs.

Adolescent↗