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Biomedical subjects

H Wu

Publications and source records attributed to H Wu.

At least 811 records · Page 45Linked to original sources
PubMed1990

[Effect of dry ginger and roasted ginger on experimental gastric ulcers in rats].

This paper reports the effect of dry ginger and roasted ginger decoctions on four experimental gastric ulcer models in rats at an oral administration of 4.5 g/kg. The result shows that the roasted ginger has an obvious inhibiting tendency on three gastric ulcer models except the indomethacin induced model while the dry ginger has no such effects. The acute toxicity test has shown that the LD50 of roasted ginger decoction administered orally is 170.6 +/- 1.1 g/kg, but it is over 250 g/kg with dry ginger. This suggests that the water soluble constituents of the dry ginger have changed in the roasting process.

Acetates↗
PubMed1990

A study on the life-cycle and epidemiology of Pseudanoplocephala crawfordi Baylis, 1927.

A study of the life-cycle and epidemiology of Pseudanoplocephala crawfordi Baylis, 1927 is reported. Intermediate hosts are Tenebrio obscurus, T. molitor, Carpophilus dimidiatus and Tribolium castaneum. The first three species are new host records. Development in the intermediate host takes 1-1.5 months. The completion of the life-cycle takes 2-2.5 months. Surveys of intermediate hosts, pigs, humans and rats were carried out. In Rattus norvegicus, the prevalence was as high as 22%. Epidemiological factors and suggestions for control measures are discussed.

Animals↗
PubMed1990

[Ultrastructures of the capsule and subcapsular epithelium of the senile cataractous lens].

The capsule and subcapsular epithelium of 18 senile cataractous lenses and 2 normal lenses were studied by scanning and transmission electron microscopy. The structures of the capsule and subcapsular epithelium in most senile cataractous lenses were similar to those in the normal lenses. Low electron density bands composed of tiny granular or fibrillar material deep in the posterior capsule were observed in 4 of the senile cataractous lenses. Degeneration was more marked in the subcapsular epithelial cells in some of the senile cataractous lenses.

Aged↗
PubMed1989

Human deoxycytidine kinase. Sequence of cDNA clones and analysis of expression in cell lines with and without enzyme activity.

Deoxycytidine kinase (dC kinase) is the rate-limiting enzyme in the anabolism of important anticancer and retroviral nucleoside derivatives. Its activity is often decreased in resistance to these drugs. To analyze the structure, function, and control of this clinically important enzyme we isolated 15 cDNA clones for human deoxycytidine kinase from lambda gt11 thymus and Molt 4 libraries. Four clones were sequenced. The largest clone is 2.9 kilobases and codes for a 626-amino acid open reading frame. The DNA and deduced amino acid sequence of the human dC kinase clones are homologous with a previously unidentified murine cDNA clone p3.4J (EMBL:MM34j) reported to be related to granulocyte-macrophage colony-stimulating factor. Deoxycytidine kinase also has cysteine-rich regions that are homologous with thioredoxin, the beta subunit of prolyl 4-hydroxylase, phosphoinositide-specific phospholipase C, thyroid hormone-binding protein, and protein disulfide isomerase. No differences were seen in the amount and size of deoxycytidine kinase protein and mRNA between CCRF/CEM and L1210 leukemic cell lines that express and do not express enzyme activity. Genomic restriction fragments were similar between the active and inactive CCRF/CEM cell lines. These data suggest that the cells deficient in dC kinase activity have a small defect in the structural gene.

Amino Acid Sequence↗
PubMed1989

Creatine kinase is modified by 2-chloromercuri-4-nitrophenol at the active site thiols with complete inactivation.

Creatine kinase modified by mercurials has been reported to be fully reactive as the native enzyme. This was ascribed to the modification of a second class of thiol groups instead of the reactive thiols at the active site (Laue, M.C. and Quiocho, F.A. (1977) Biochemistry 16, 3838-3845). It has now been shown by spectrophotometric titration and fluorescence studies that 2-chloromercuri-4-nitrophenol (MNP) reacts preferentially with the active-site thiol. Moreover, if the activity of the modified enzyme is determined in the absence of added bovine serum albumin or other enzymes, as usually employed in coupled activity assay systems for creatine kinase, the modified enzyme is completely inactive. Addition of an excess of bovine serum albumin or rabbit muscle glyceraldehyde-3-phosphate dehydrogenase restores the activity of the enzyme to over 80% of its original level. It appears that the active thiol groups at the active site of creatine kinase are after all modified by MNP with complete inactivation.

Animals↗
PubMed1989

[Imaging and biodistribution of 111In-labeled anti-carcinoembryonic antigen monoclonal antibodies in nude mice bearing human colon cancer xenografts].

Anti-CEA monoclonal antibody was labeled with 111In using DTPA cyclic anhydride. The radiochemical purity of the product was more than 99% and specific binding was 38%. Imaging and biodistribution of this radiopharmaceutical in nude mice bearing human colon cancer xenografts were investigated. The results showed that a clear image of tumor was obtained by gamma camera between 24 to 120 hrs, best in 72 hrs, after injection. A high uptake of 111In-labeled monoclonal antibodies in tumor tissue was also observed, although the radioactivity in liver was considerable.

Animals↗
PubMed1989

[Observation on the development of Plasmodium falciparum in Anopheles dirus].

This paper is a record of our observation on the stages of development of Plasmodium falciparum in Anopheles dirus. The malarial parasites were derived from 5 infected patients living in Guizhou Province and used to infect 8 batches of An.dirus. The morphology of various developmental stages studied under light microscope and their average size were as follows: Microgametes were filament-shaped, 13.31 +/- 2.22 microns; macrogametes and zygotes oval or round, 4.36 +/- 0.59 microns and 3.39 +/- 0.39 microns respectively; ookinetes banana shaped, 13.56 +/- 0.80 microns x 2.90 +/- 0.48 microns; oocysts ovoid or spherical in shape, the smallest one being 7.086 microns in equivalate diameter (2 days old) and the largest one 72.60 microns (11 day old); slender sporozoites measured 10.625 +/- 0.82 microns x 1.179 +/- 1.3 microns. The late sporogonic stage of P. falciparum was observed with scanning electron microscope. The sporozoite buds developed on the surface of the sporoblast bodies, being round or elliptic or irregular-shaped. The anterior end of sporozoites was truncate and sometimes a micropyle could be seen at a distance 1/3 from the anterior end. A description was given of the different characteristics of the macrogametes and zygotes, together with the arrangement of the pigment granules of oocysts.

Animals↗
PubMed1988

Guanine nucleotides inhibit agonist-stimulated arachidonic acid release in both intact and saponin-permeabilized human platelets.

The effects of guanine nucleotides on arachidonic acid (AA) release were studied in intact and saponin-permeabilized human platelets. While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agonists in intact, as well as permeabilized platelets. Inhibition of agonist-stimulated AA release by nucleotides was partially attenuated by addition of ADP, and was abolished by prior stimulation of platelets to discharge the ADP-containing dense granules. These results suggest: (i) that released ADP plays an important contributory role in agonist-stimulated platelet AA release, and (ii) that guanine nucleotides can modulate platelet activation through an extracellular action which is distinct from their effects on G-proteins.

Arachidonic Acid↗