Search PubMed⌕ Search

Biomedical subjects

H Woelk

Publications and source records attributed to H Woelk.

62 records · Page 4Linked to original sources
PubMedDate not supplied

Benfotiamine in treatment of alcoholic polyneuropathy: an 8-week randomized controlled study (BAP I Study).

A three-armed, randomized, multicentre, placebo-controlled double-blind study was used to examine the efficacy of benfotiamine vs a combination containing benfotiamine and vitamins B6 and B12 in out-patients with severe symptoms of alcoholic polyneuropathy (Benfotiamine in treatment of Alcoholic Polyneuropathy, BAP I). The study period was 8 weeks and 84 patients fulfilled all the prerequisite criteria and completed the study as planned. Benfotiamine led to significant improvement of alcoholic polyneuropathy. Vibration perception (measured at the tip of the great toe) significantly improved in the course of the study, as did motor function. and the overall score reflecting the entire range of symptoms of alcoholic polyneuropathy. A tendency toward improvement was evident for pain and co-ordination; no therapy-specific adverse effects were seen.

Adult↗
PubMedDate not supplied

The Ca2+-dependent incorporation of nitrogenous bases into brain microsomal phospholipid subspecies in vitro.

The specificity of the Ca2+-stimulated choline and ethanolamine incorporation into the molecular subspecies of the correspondent choline and ethanolamine phosphoglycerides has been investigated in vitro in rat brain microsomes. In the presence of 5.0 mM Ca2+-ions and at pH 8.1, choline was incorporated 6 times faster into the tetraenoic diacyl-glycero-3-phosphorylcholines (diacyl-GPCs or lecithins) than into the saturated subspecies. The specific activities of the other species were intermediary, and decreased with increasing saturation. Hexaenoic species of lecithins were however weakly labelled. The rate of labelling of diacyl-GPC molecular subspecies was affected noticeably by changing the pH and the Ca2+-ion concentration of the incubation medium. Ethanolamine was incorporated in the presence of 2.5 mM Ca2+-ions and at pH 8.1 preferentially into the monoenoic species of total ethanolamine phosphoglycerides of rat brain microsomes. The rate of incorporation into the monoenoic species was twice that into the trienoic, tetraenoic and hexaenoic and 4 times faster that into the dienoic species. When the pattern of labelling was determined specifically for the molecular subspecies of diacyl-glycero-3-phosphorylethanolamines (diacyl-GPEs or phosphatidylethanolamines), the rate of incorporation of ethanolamine into the hexaenoic species resulted three times faster that into the saturated and monoenoic species and about twice that into the trienoic and tetraenoic species, in accordance with data for liver microsomes. The pattern of labelling of the molecular subspecies of ethanolamine phosphoglycerides and of diacyl-GPEs was not influenced by changing the pH and the Ca2+-ion concentration of the incubation medium.

Animals↗
PubMedDate not supplied

The influence of soybean diacylglycerol on brain phospholipid synthesis during aging.

The synthesis of choline phosphoglycerides and ethanolamine phosphoglycerides has been examined in brain cortex microsomes and neuronal populations of rats during various times of aging as compared to 2 months-old animals. A noticeable decrease of synthesis of both lipid classes takes place in these conditions in vitro. The concentration of the endogenous diacylglycerol is not changed during aging neither in microsomes nor in neurons. The molar distribution of fatty acids in brain microsomal diacylglycerols of aging rats is noticeably different from that of adult animals. The content of monoenoic and dienoic species is increased, whereas that of the tetraenoic species is decreased. The addition of soybean diacylglycerol to microsomes or neuronal preparations from aged rat brain restores practically the decreased synthesizing activity of CPG and partially that of EPG. It is proposed that adding diacylglycerols to aged membranes affects the properties of the membranes and probably enzyme-lipid interactions.

Aging↗