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Biomedical subjects

H Witas

Publications and source records attributed to H Witas.

22 records · Page 2Linked to original sources
PubMed1978

A simple and rapid procedure for isolation of ceruloplasmin from porcine and bovine blood serum.

A rapid and simole procedure for isolation of ceruloplasmin from porcine and bovine blood sera was elaborated; it involves DEAE-Sephadex A-50 chromatography, precipitation with an ethanol-chloroform mixture plus extraction with saline, and preparative polyacrylamide-gel electrophoresis. The preparations obtained showed a high degree of electrophoretic homogeneity and a higher A610/A280 ratio and specific activity than the preparations obtained by the modified method of Broman & Kjellin (Biochem. Biophys. Acta, 82, 101-109, 1964).

Animals↗
PubMed1977

Changes of adenine nucleotides content and release reaction of human blood platelets following gamma irradiation.

The aim of this work was to examine the effect of gamma irradiation on the energy metabolism and physiological functions of blood platelets. Blood platelets were irradiated with a 60Co-source in the range of 0.5--8.0 krad. Before and after irradiation, the free nucleotide content of platelets and the ability of platelets to perform their hemostatic functions (the release reaction with thrombin) were determined. The obtained results demonstrated that: 1. ATP, ADP and AMP content reached minimum values at 1.5--2.0 krad. 2. The ability of platelets to perform the release reaction correlates with the ATP level at doses of 1.5--2.0 krad. At higher doses (6.0--8.0) disturbances of the release reaction, indicating damage to the platelet plasma membranes, were observed.

Adenine Nucleotides↗
PubMedDate not supplied

Lipids associated with dog gastric mucus glycoprotein.

The lipid content and composition of dog gastric mucus and its mucus glycoprotein before and after Pronase digestion was investigated. The mucus and the purified glycoprotein, in its intact and degraded form, were extracted with chloroform/methanol and the lipids contained in the extracts were quantitated. The lipids accounted for 18.9% of the dry weight of mucus and their content increased to 29.4% in the purified glycoprotein and fell to 27.8% following digestion of the glycoprotein with Pronase. In comparison to mucus, lipids associated with undegraded glycoprotein contained 1.7 times more phospholipids and 1.8 times more glycolipids, and were virtually free of glycosphinoglipids. Pronase digestion of the glycoprotein led to 2.3-fold decrease in phospholipids and 1.3-fold increase in glyceroglucolipids. The results suggest that the interaction of mucus glycoprotein with phospholipids involves its nonglycosylated region.

Animals↗