Biomedical subjects
Publications and source records attributed to H Wiesinger.
Eleven proximal pseudarthroses of the tibia and elevated osteotomies of the head of the tibia were treated by fitting a condylar plate; this was done at two hospitals in Berlin and Bayreuth in the course of the past ten years. In all osteosyntheses, osseous reconstruction was achieved within a favourable time. Although mounting is technically intricate, it yields immediate or at least early exercise stability.
Non-unions of the neck of the femur are still observed today in spite of all the improvements which have been made in the surgical treatment of median fractures of the neck of the femur. Linear osteotomy according to Pauwels is technical relatively simple to perform and yields good results. Such linear osteotomy is performed in children and young adults only even if the head of the femur is not rounded. Indication for alloplasty is now more liberal even in relatively young adults since cement-free implantation of total prostheses has proved successful. Indication for performing replacement of the head alone, is very rare. Palliative measures, such as Girdlestone or resection angulation osteotomy are performed in our patients subsequent to septic complications only.
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Association of the apo-beta 2 and the holo-(beta-PLP)2 subunits of tryptophan synthase from Escherichia coli (L-serine hydro-lyase (adding indole) (EC 4.2.1.20)) with alpha subunits of the same enzyme has been studied by microcalorimetry. The results obtained from thermometric titrations clearly demonstrate that only the native complex alpha2beta 2 is formed, independent of an excess of alpha protein. The reaction of the holo-(beta-PLP)2 with alpha subunits at 25 degrees C is accompanied by a negative enthalpy change, which is almost twice as large as that for complex formation with the apo-beta 2 protein, thus indicating that the interaction enthalpy becomes more favorable in the presence of the coenzyme pyridoxal 5'-phosphate (PLP). Both reaction enthalpies show very large negative temperature coefficients, -3600 +/- 100 cal K-1 (Mol of beta 2)-1 being the value for the formation of the apoenzyme and -2300 +/- 100 cal K-1 (mol of beta 2)-1 pertaining to formation of the holoenzyme. The studies on the association of alpha and beta2 subunits in the two buffers revealed that at 25 degrees C approximately 0.75 proton are absorbed in the presence and absence of the coenzyme, whereas at 35 degrees C one proton is taken up from the solution when PLP is present, but two if the apo-beta 2 complex reacts. These results are a clear indication of energetic linkage between intersubunit interaction, hydrogen ion equilibria, and the binding of the coenzyme.
For a period of 8 days, pregnant white New Zealand rabbits were treated either with the beta-sympathomimetic Ritodrine (1 mg/kg) body weight daily) or with 0.9% saline solution. The fetuses were delivered by caesarian section on the 25th and 30th day of gestation (term being 31 days). No significant difference was found between the Ritodrine and saline treated groups with respect to the levels of dipalmitoyl-lecithin (DPL) obtained from the homogenates as well as the alveolar lavages of the fetal lungs. These results indicate that with this experimental model, prepartum treatment with Ritodrine has no effect either on DPL synthesis or its release into the alveolar spaces. Furthermore, treatment with the beta-sympathomimetic produced no significant change in the concentrations of the specific glucocorticoid receptors in the cytosol and nuclei of the fetal lungs, and in addition, the lung cytosol glucose concentrations as well as the plasma corticosteroid levels were unaffected. These findings support the conclusion that the synthesis of DPL in the fetal lung is not stimulated by Ritodrine.
Nine pure-bred albino New Zealand white rabbits were delivered on the 30th day of gestation (term 31 days) by Caesarean section. The concentration of dexamethasone binding receptors in the cytosol and nucleus of the lungs as well as the total plasma corticosteroid levels were measured in the newborns before the first breath, after 30 min, 1, 2, 4, 6 and 24 h after birth. The level of plasma corticosteroids increased after birth while the concentration of the receptors in the cytosol and nucleus decreased but was still present after 24 h as well as in the lungs of adult animals. A temporary increase in the concentration of the nuclear receptors after 1 and 2 h is explained by the translocation and binding of the abundantly produced steroid-receptor-complex to the nucleus. The stress of hypo- and hyperthermia on the newborns showed variable behaviour with respect to receptor activity. However, the relationship between the corticosteroid levels in the plasma and the concentrations of the receptors in the cytosol and the nucleus of the lungs was similar as in fetuses kept under "physiological" conditions. It may be concluded from these results that in the rabbit the specific glucocorticoid receptor activity in the lungs decreases after birth but is present throughout life.
A case is presented in which bilateral spontaneous choroidal detachments appear to be the direce result of bilateral dural arteriovenous fistula of the cavernous sinus region. Rapid resolution of the clinical signs followed bilateral orbital decompression via the transfrontal approach. Similarities in clinical presentation of both entities are reviewed and a premise for their cause-and-effect relationship elaborated. A literature search for similar unrecognised cases is discussed. The paper suggests that this association may be more frequent than published reports would imply.
Recently, the radioimmunological determination of C-peptide came into interest because of the jugdement of the remaining function of the islet apparatus in insulin-dependent diabetics. As the degradation of C-peptide preferably takes place in the kidney we performed an intravenous glucose load in 32 patients with kidney diseases. The following results were obtained: 1. In patients with a healthy carbohydrate metabolism a clear correlation exists between the concentration of creatinine on the one hand, the creatinine-clearance and the fasting C-peptide concentration respectively the measured amount of C-peptide on the other hand. 2. The more advanced the renal insufficiency the better is the correlation between the parameter of the kidney function and the C-peptide concentration. 3. In diabetic patients there shows to be no clear correlation between the C-peptide levels and the kidney function. --In insulin-dependent diabetics the amount of C-peptide is only of diagnostic use if the renal function is well known.
The effect of electric current on the eyes of experimental animals was studied. An apparatus was constructed to allow single and multiple shock exposures from 0 to 600 volts at variable exposure times. The current was measured on a Tectronex Storage Oscilloscope. Mature Dutch rabbits were used as experimental animals. When rabbit eyes were subjected to a current of 500 volts for no less than 250 milliseconds and 400 milliamperes or more, a permanent decrease in the amplitude of the b-wave of the Electroretinogram resulted in some animals. No histological or ophthalmological alterations in the retina, optic nerve or vasculature could be observed using conventional techniques. The cataractogenic properties of electric current were also studied. Most previous observations dealing with this subject utilized a multiple shock technique--something which is unlikely to occur in any accidental exposure. This study focussed on the production of lens changes in rabbit eyes following single exposures of measured electric current. The biomicroscopic characteristics of these changes were described. Minimal lens changes were produced by single shock exposures ranging from 6 to 26 watts per second while typical electrical anterior subcapsular cataracts were produced by current of 23 to 80 watts per second. Exposure times were mostly 250 milliseconds or less since the survival rate of animals subjected to longer exposures made such studies unfeasible. Three patients with electrical injuries were presented and some of the pertinent literature was reviewed.
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