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Biomedical subjects

H Wen

Publications and source records attributed to H Wen.

At least 73 records · Page 4Linked to original sources
PubMed1997

IgG1 and IgG4 serum antibody responses in asymptomatic and clinically expressed cystic echinococcosis patients.

IgG1 and IgG4 subclass antibody responses were investigated in two clinically different groups of hepatic cystic echinococcosis (CE) patients. One group consisted of surgically proven CE cases (clinically expressed hospitalized cases) and a second group comprised asymptomatic CE patients (first time community detected cases) diagnosed by portable ultrasound and serology in four different endemic communities. Fifty eight sera from surgically proven CE patients and 133 sera from asymptomatic ultrasound diagnosed CE cases were screened by ELISA using purified human hydatid cyst fluid antigen B for total specific IgG, IgG1 and IgG4 antibodies. Fifty sera from healthy individuals from within endemic regions were used as control negatives. Compared to control negatives total IgG antibody levels were elevated in both surgically proven (85%) and asymptomatic CE cases (77%) but there was no significant difference between the two groups. IgG1 subclass antibody levels were also elevated in both surgically proven (55%) and asymptomatic cases (58%) compared to endemic controls and similarly the difference in this response was not significant between these two groups. In contrast the prevalence of IgG4 antibodies in surgically confirmed chronic CE patients was greater (71%) than the respective IgG1 antibody levels (55%). The greatest difference in specific antibody response, between advanced surgically confirmed CE patients and ultrasound detected asymptomatic CE cases, was observed for IgG4 antibody levels which were detected in 71% of symptomatic compared to only 23% of asymptomatic patients (P < 0.0001). These observations confirm that IgG4 is an important diagnostic parameter for clinically expressed cystic echinococcosis in humans and suggest that a switch from a predominant IgG1 response to IgG4 might occur in CE patients as the disease progresses.

Animals↗
PubMed1997

The intrinsic signal-to-noise ratio in human cardiac imaging at 1.5, 3, and 4 T.

Cardiac imaging is inherently demanding on the signal-to-noise performance of the MR scanner and may benefit from high field strengths. However, the complex behavior of the radiofrequency field in the human body at high frequencies makes model-based analyses difficult. This study aims to obtain reliable comparisons of the signal-to-noise profile in the human chest in vivo at 1.5, 3, and 4 T. By using an RF-field-mapping method, it is shown that the intrinsic signal-to-noise increases with the field strength up to 4 T with a less than linear relation. The RF field profile is markedly distorted at 4 T, and the onset of this distortion is dependent on the body size. The high power deposition and the consequences of the RF field distortion are discussed.

Algorithms↗
PubMed1997

Simulation of B1 field distribution and intrinsic signal-to-noise in cardiac MRI as a function of static magnetic field.

Two issues that pertain to the optimal static magnetic field for cardiac MRI were addressed: intrinsic signal-to-noise ratio (ISNR) and radiofrequency power deposition. From 1.5 to 9.5 T, proton Larmor frequencies of 63 to 400 MHz, numerical simulations were performed of the RF fields from a surface coil and a body coil loaded by a heterogeneous, three-dimensional, symmetric model of the human chest. The RF field distribution, the power required to produce the RF field, and the ISNR at the center of the heart were computed. The model was validated by comparison with experimental data up to 4 T. The RF field distortion was quantified and found to increase linearly up to 6 T due mostly to dielectric resonance modes. Body coil simulations beyond 6 T showed the onset of higher-order modes at the center of the heart. A range of expected RF power requirements was constructed as a function of field up to 9.5 T for surface coils and up to 6.8 T for body coils. Over this range of static field, ISNR for a constant coil geometry was bracketed by an upper limit that was slightly greater than linear with field and a lower limit that was slightly less than linear with field. The RF power and ISNR showed a strong dependence on chest thickness at 1.5 and 4.0 T. Additionally, independent of chest thickness, the model predicts a lower limit of a factor of 5 increase in RF power as the static field is increased from 1.5 to 4 T. Implications for imaging with other nuclei are discussed. Methods for checking the self-consistency of electrodynamic simulations are presented.

Algorithms↗
PubMed1997

A pilot, serological survey for cystic echinococcosis in north-western Mongolia.

The seroprevalence of cystic echinococcosis in north-western Mongolia was determined in a cross-sectional study of 334 semi-nomadic pastoralists in their summer grazing areas. Overall, 5.2% of the subjects were found to be strongly seropositive for antibodies to Echinococcus granulosus antigen B by ELISA. Only 10% of the adult subjects (aged > or = 15 years) had heard of the disease and only 5% recognized hydatid cysts in their livestock (in contrast to the neighbouring province in China, where 76% had seen and recognized cysts in the organs of slaughtered animals). The close association between Mongolian pastoralists, their dogs and livestock is likely to encourage ready transmission of the parasite in a region where home slaughter is practised and offal for various scavengers, including domestic dogs. Further information is needed to determine the prevalence of infection in other intermediate hosts and in dogs and the clinical importance of the disease in this remote region.

Adolescent↗
PubMed1997

Small-dose itraconazole pulse therapy in the treatment of onychomycosis.

The aim was to evaluate efficacy and tolerance of a short-schedule treatment regimen using a small dose of itraconazole in pulse intermittently. The open study evaluated the pulse therapy consisting of monthly 1-week cycles of oral itraconazole 200 mg daily for 2-3 consecutive months in 42 patients with onychomycosis. After active therapy, patients were evaluated for a maximum period of 1 year. Twelve of 42 patients were considered as being clinically cured, 17 were markedly improved, 11 were improved and two were failures. A mycological examination at the final visit was performed on all patients. Thirty-five were negative and seven were positive. This short treatment was well tolerated, with no adverse reactions, and may offer a new option for treatment of onychomycosis.

Administration, Oral↗
PubMed1997

Antibody responses against Em18 and Em16 serodiagnostic markers in alveolar and cystic echinococcosis patients from northwest China.

Western blot analysis was carried out in order to evaluate new serodiagnostic markers, Em18 and Em16, for differentiation of alveolar echinococcosis (AE) from cystic echinococcosis (CE) using 36 serum samples from hydatid patients from Xinjiang, China, where AE and CE are both endemic and one double infection case has been reported. All AE cases except one (5/6) who exhibited a calcified lesion and a single case of double infection showed antibody responses against Em18 and Em16. Some of CE patient sera (6/22) showed antibody response against Em16 except one who showed that against Em18. Analyses of IgG subclass responses against Em18 and Em16 were carried out using all serum samples showing antibody responses against Em18 and/or Em16 (seven CE, five AE, and one AE + CE) and additional samples of three CE and 22 AE from Sichuan, China. IgG4 was the most predominant antibody subclass. Em18 and Em16 were recognized by both IgG4 and IgG1 (in most cases) or by either IgG4 or IgG1 (in minor cases) or by IgG3 (in very rare cases). Neither Em18 nor Em16 was recognized by IgG2 antibodies. The usefulness of Em18 and Em16 as potential new markers for serological differentiation of human AE and CE, respectively, is discussed.

Antibody Formation↗
PubMed1997

[Plasma and skin tissue endothelin in patients with psoriasis vulgaris].

The levels of plasma endothelin in 46 patients with psoriasis vulgaris and the levels of endothelin in skin tissue of 25 cases were determined by RIA. The results showed that: (1) The plasma levels of endothelin in patients with psoriasis vulgaris was significantly increased compared with that of the controls (P < 0.01), so was the active stage group compared with the stable stage group (P < 0.01); (2) The level of endothelin in skin lesions of patients was significantly higher than that of the uninvolved skin. These findings suggest that endothelin might play a role in the pathogenesis of psoriasis vulgaris.

Adolescent↗
PubMed1997

[Determination on the glycosyl sequence of gypenoside A by TLC-FABMS].

The glycosyl sequence of gypenoside A (A) isolated from Gynostemma pentaphyllum Makino was determinated by TLC-FABMS. The gypenoside A was hydrolysed with cellulase and the hydrolysate showed eight spots on a TLC plate, and the FABMS analysis of these spots indicated they corresponded to gypenoside A, A-Rham, A-Glu, A-Rham-Glu, A-Rham-Glu, A-Glu-Glu, A-Rham-2Glu, A-Rham-2Glu in order. Two chains can be determined to be Rham-Glu, and Glu-Glu, respectively. So the glycosyl sequence of gypenoside A was identical of gypenoside XLIII.

Chromatography, Thin Layer↗
PubMed1997

[Prognosis and prognostic factor analysis epithelial ovarian cancer].

OBJECTIVE: To investigate the prognosis change and prognostic factors of advanced epithelial ovarian cancer in recent over twenty years. METHODS: One hundred and fourty patients with advanced epithelial ovarian cancer were analyzed. They were divided into two groups based on their operation time. 56 patients operated before Jan. 1980 were included in the first group, and the other 84 patients in the second group. Kaplan-Meier survival curves of the two groups were calculated, and Cox model was used to evaluate the prognostic factors of the disease at univariate and multivariate levels using SPSS and SURVCALC software. RESULTS: There was no pathological difference in between the two groups, but the patients in the second group received more aggressive chemotherapy. The overall 1-, 2-, and 5-year survival rates were 61.2%, 32.1%, and 8.5%, respectively. In the first group they were 42.3%, 29.6% and 4.5% respectively and in the second group 69.3%, 36.2%, and 11.2% respectively. The prognosis of the patients in the second group was better than that in the first group, P < 0.05. Univariate analysis revealed that stage, grade, residual tumor size and chemotherapy were significant predictors of patients' outcome. COX regression analysis identified that they were all independent prognostic factors. The patients with advanced, low grade, residual tumor (> 2 cm) had poor prognosis. The prognosis of the patients who received equal or more than four, especially six cycles combined chemotherapy was improved. CONCLUSION: The prognosis of the patients with advanced ovarian cancer has improved in recent over 10 years, higher optimal cytoreductive rate and equal or more than six cycles combined chemotherapy are important measures for the better prognosis of advanced ovarian cancer.

Adenocarcinoma, Mucinous↗
PubMed1997

[The clinical and pathological characteristics of epithelial ovarian carcinoma in patients with age under 40].

OBJECTIVE: To investigate the clinical and pathological characteristics of epithelial ovarian carcinoma in patients with age under 40. METHODS: From Jan 1978 to Dec 1992, 54 cases with epithelial ovarian carcinoma under 40 years old were admitted to our hospital and enrolled in the study group, another 54 patients suffered from the same disease with age over 40 in the same period were served as control. Comparison analysis for clinical and pathologic data of 2 groups was performed using SPSS and SURVALC statistics software. RESULTS: Main complaint because of either pelvic mass or abdominal mass found by chance was more common in study group (46.3%) than that in control group (27.8%). whereas because of symptoms was less common in study group (53.7%) than in control group (72.2%). Other parameters such as early stage (I and II) rate (61.1%), unilateral tumor rate (68.5%), average maximum diameter of tumor (13.6 cm), highly differentiated rate (50%) and the possibility for tumor reductive surgery were higher in study group than in control group. The difference of histological pattern and chemotherapy between 2 groups was not significant. Single variate analysis showed that the prognosis was better in study group. The 2-year and 5-year survival rates were 69.8% and 50.2% respectively. No recurrence was found in the 8 patients in whom the contralateral healthy ovary remained. However multiple variate analysis indicated that age was not a prognostic factor. CONCLUSION: Epithelial ovarian carcinoma was prone to be lower degree malignancy in patients under 40 years therefore the prognosis of them was better. It might be resonable to have healthy ovary left in some select cases.

Adolescent↗
PubMed1996

A method to improve the B0 homogeneity of the heart in vivo.

A homogeneous static (B0) magnetic field is required for many NMR experiments such as echo planar imaging, localized spectroscopy, and spiral scan imaging. Although semi-automated techniques have been described to improve the B0 field homogeneity, none has been applied to the in vivo heart. The acquisition of cardiac field maps is complicated by motion, blood flow, and chemical shift artifact from epicardial fat. To overcome these problems, an ungated three-dimensional (3D) chemical shift image (CSI) was collected to generate a time and motion-averaged B0 field map. B0 heterogeneity in the heart was minimized by using a previous algorithm that solves for the optimal shim coil currents for an input field map, using up to third-order current-bounded shims (1). The method improved the B0 homogenelty of the heart in all 11 normal volunteers studied. After application of the algorithm to the unshimmed cardiac field maps, the standard deviation of proton frequency decreased by 43%, the magnitude 1H spectral linewidth decreased by 24%, and the peak-peak gradient decreased by 35%. Simulations of the high-order (second- and third-order) shims in B0 field correction of the heart show that high order shims are important, resulting for nearly half of the improvement in homogeneity for several subjects. The T2* of the left ventricular anterior wall before and after field correction was determined at 4.0 Tesis. Finally, results show that cardiac shimming is of benefit in cardiac 31P NMR spectroscopy and cardiac echo planar imaging.

Echo-Planar Imaging↗
PubMed1996

The evaluation of dielectric resonators containing H2O or D2O as RF coils for high-field MR imaging and spectroscopy.

Electromagnetic resonators consisting of low-loss dielectric material and/or metallic boundaries are widely used in microwave technologies. These dielectric resonators usually have high Q factors and well-defined field distributions. Magnetic resonance imaging was shown as a way of visualizing the magnetic field distribution of the resonant modes of these resonators, if the dielectric body contains NMR sensitive nuclei. Dielectric resonators have also been proposed as RF coils for magnetic resonance experiments. The feasibility of this idea in high-field MR is discussed here. Specifically, the dielectric resonances of cylindrical water columns were characterized at 170.7 MHz (4 T 1H Larmor frequency), and evaluated as NMR transmit and receive coils. The dielectric resonance of a cylindrical volume of D2O was used to image a hand at 170.7 MHz. This study demonstrated that MRI is an effective way of visualizing the magnetic field in dielectric structures such as a water cylinder, and can potentially be generalized to solid-state dielectric devices. The possible applications of dielectric resonators other than simple cylindrical volumes in MRI and MR solution spectroscopy at high field strengths are also discussed.

Deuterium↗
PubMed1996

Pharmacology and efficacy of liposome-entrapped albendazole in experimental secondary alveolar echinococcosis and effect of co-administration with cimetidine.

Encapsulation of the benzimidazole albendazole in multilamellar liposomes results in a preparation in which this normally insoluble anti-hydatid drug is well solublilized in aqueous media. The high entrapment efficiency observed (75-87%) and the stability of the formulation make this a promising delivery vehicle for improved chemotherapy with albendazole. In particular, the high degree of association with phospholipid may give rise to increased oral bioavailability. Oral administration of albendazole in liposomes led to increased concentration and/or altered metabolism of albendazole sulphoxide (ABZSX) in liver and/or plasma in non-infected Wistar rats. Results from experiments using cotton rats (Sigmodon hispidus) infected with metacestodes of Echinococcus multilocularis show that entrapment within liposomes clearly increases the uptake of albendazole via the oral route. This was reflected by increased levels of albendazole and the two major metabolites in plasma, liver and cyst homogenate when a dose of liposomal albendazole (35 mg/kg) was given orally compared to free albendazole at 50 mg/kg. There was a 75-94% reduction in biomass of the metacestode and a significant increase in survival time for the animals treated with liposome entrapped albendazole. A clear difference in distribution of albendazole and its metabolites in the liver and the metacestode tissues in the presence of cimetidine indicated that the latter has a profound effect on the metabolism of albendazole. There appeared to be a synergistic interaction between albendazole and cimetidine, since the metabolism of albendazole was markedly altered in the combined cimetidine/ liposome-albendazole group, and higher therapeutic effect was observed. These findings indicate potential both for improvement of treatment of larval E. multilocularis infection and for reduction of albendazole dose levels.

Albendazole↗
PubMed1996

MR imaging contrast in human brain tissue: assessment and optimization at 4 T.

PURPOSE: To evaluate multiple magnetic resonance (MR) imaging sequences for their ability to provide T1 and T2-weighted images at a field strength of 4 T, and to validate previously obtained relaxation time measurements. MATERIALS AND METHODS: Different spin-echo, inversion-recovery (IR), gradient-recalled acquisition in the steady state (GRASS), and magnetization transfer contrast-enhanced GRASS sequences were evaluated in a single section, each in at least four volunteers. Also, interleaved echo-planar imaging (IEPI) and interleaved gradient-recalled echo (IGRE) sequences were analyzed and compared with standard sequences. RESULTS: Predicted contrast behavior, according to MR relaxation time measurements, was found to agree well with that of the validation experiments. Fair T1 contrast can be achieved on MR images at 4 T, contrary to early predictions. Under other conditions, however, such as partially spin-density-weighted parameters, lower contrast is observed at the high field strength when compared with conventional field strengths. CONCLUSION: The longer T1 values at higher field strengths have a substantial effect on image contrast. Depending on the sequence and parameters chosen, good image contrast can be realized at 4 T. IEPI and hybrid IR IGRE sequences are useful in shortening the prolonged examination times owing to the longer T1 values at higher field strengths.

Artifacts↗