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Biomedical subjects

H Welzl

Publications and source records attributed to H Welzl.

At least 37 records · Page 2Linked to original sources
PubMed1992

The effects of anticholinergic drugs on delayed time discrimination performance in rats.

To investigate the effects of the muscarinic antagonist scopolamine and the nicotinic antagonist mecamylamine on time discrimination and short-term memory, rats were trained on a delayed conditional time discrimination task until performance stabilized. In a two-lever operant chamber, pressing one lever was correct after the presentation of a stimulus light for 2 seconds (SD short); and pressing the other lever was correct after presentation of a stimulus light for 8 seconds (SD long). Scopolamine (0.06 mg, 0.25 mg, and 1.0 mg/kg) attenuated performance in a dose-dependent manner. Furthermore, the drug decreased nose-pokes (an activity necessary to trigger the presentation of the discriminative stimuli and the presentation of the response levers), and increased response delay (time from opportunity to lever press to actual lever press). Performance attenuating effects of mecamylamine in the time discrimination task did not appear unless high doses (8 mg/kg) of the drug were given. With increasing delays between 0 and 8 seconds, animals injected with saline developed a bias towards reporting the occurrence of the SD long, independent of the actual stimulus presented. A reversal of this bias was seen in animals injected with scopolamine; they more often reported the occurrence of the SD short. Our results support a role for muscarinic receptors in discrimination learning, attention, and time estimation.

Animals↗
PubMed1991

Prenatal stress effects on exploratory activity and stress-induced analgesia in rats.

Pregnant rats were exposed three times daily to immobilization stress during gestational Days 15-19. The behavior of their offspring was compared with the behavior of offspring from unstressed control mothers. Although the stress procedure decreased the weight gain of mothers during pregnancy, it slightly but significantly increased the weight of their offspring at birth and at weaning. On postnatal Day 10, prenatally stressed pups returned to their home cage more quickly than did prenatally unstressed control pups during a nest odor discrimination task, but no differences between groups in the number of correct responses were found. On postnatal Days 70-72, prenatally stressed offspring showed increased exploratory activity in a complex tunnel maze compared with control offspring. On postnatal Day 80, analgesia induced by stress (swimming for 3 min in cold water) was determined (tail flick latency). The degree of stress-induced analgesia was smaller in prenatally stressed rats than in control rats. These data suggest that the effects of prenatal stress on behavior are most clearly discernable when such animals are confronted with a novel or stressful situation.

Animals↗
PubMed1991

Effects of nicotine, caffeine, and their combination on locomotor activity in rats.

The interactive effect of caffeine and nicotine on spontaneous locomotor activity in a tunnel maze was determined in nicotine-naive and nicotine-tolerant rats. Rats were daily injected subcutaneously for 12 days with nicotine (0.4 mg/kg) to induce nicotine tolerance. Nicotine-naive rats were injected with saline. During the next two days, they were exposed to a tunnel maze for two 6-min trials. On the third day, locomotor activity was measured (30-min trial) in the tunnel maze 15 minutes after subcutaneous injection of saline, nicotine (0.2 mg/kg), caffeine (8 mg/kg), or nicotine (0.2 mg/kg) and caffeine (8 mg/kg) in combination. Acute exposure to nicotine decreased locomotor activity in nicotine-naive rats. This decrease was antagonized by simultaneous injection of caffeine. Chronic nicotine exposure induced the development of tolerance to the acute behavioral depressive effects of nicotine. In nicotine-tolerant rats, caffeine and nicotine in combination significantly increased locomotor activity above saline level, whereas given alone they had no significant stimulant effect. Neither chronic nicotine treatment nor acute drug treatments affected exploratory efficiency of rats.

Animals↗
PubMed1990

Acute effects of nicotine injection into the nucleus accumbens on locomotor activity in nicotine-naive and nicotine-tolerant rats.

To assess the role of nicotine receptors in the nucleus accumbens on locomotor activity we bilaterally implanted guide cannulae for later injection of (-)-nicotine into the nucleus accumbens of Wistar rats. Motor activity was tested in a complex tunnel maze equipped with photocells for automatic recording. This system of dark tunnels elicits spontaneous exploration even after repeated exposure. Half of the rats were made nicotine-tolerant by daily systemic injections of (-)-nicotine for 15 days (nicotine pretreatment); the other half remained nicotine-naive (saline pretreatment). Whereas (-)-nicotine (40 nmol/0.3 microliter) bilaterally injected into the nucleus accumbens of nicotine-naive animals suppressed locomotor activity, the same amount injected into the nucleus accumbens of nicotine-tolerant rats had no effect on locomotor activity. Systemic injections of nicotine (0.4 mg/kg) induced a depression and stimulation of locomotor activity in saline-pretreated and nicotine-pretreated rats, respectively. Our results support a dual role for nicotine in locomotor activity with the initial depressant effect in nicotine-naive animals due to stimulation of the nucleus accumbens and perhaps other structures.

Animals↗
PubMed1989

Self-administration of small amounts of morphine through glass micropipettes into the ventral tegmental area of the rat.

A micropipette-system was used to investigate intracranial self-injection of morphine in the rat. The system consisted of a glass micropipette (tip dia 5 microns) connected by flexible tubing and a swivel to a pressure source. Using nose-poking through a hole in one wall of the cage as the operant, rats self-injected quantities as small as 50 ng/5 nl of morphine into the ventral tegmental area. In contrast, rats in the saline-treated group and yoked-control group did not increase their rates of nose-poking behaviour above baseline levels. Intermittent reinforcement in the morphine-treated group resulted in a further increase in the rate of nose-poking. Histological inspection of the site of injection revealed minimal damage to brain tissue and likely confinement of the injected solution to the area around the tip of the pipette.

Animals↗
PubMed1989

Effects of ketamine on tunnel maze and water maze performance in the rat.

The NMDA receptor, which has been implicated in memory formation, is noncompetitively blocked by ketamine. The present study examines the effect of ketamine (0, 3, 6, 12, and 25 mg/kg body wt; ip) on tunnel maze and water maze performance in Wistar rats. In the hexagonal tunnel maze (HTM) high doses of ketamine (12 and 25 mg/kg) decreased locomotor activity. Moreover, ketamine induced perimeter walking (6, 12, and 25 mg/kg) and attenuated exploratory efficiency (25 mg/kg). When the HTM was converted into a modified six-arm radial maze, ketamine impaired short-term but not long-term memory. In the Morris water maze, rats injected with ketamine (12 and 25 mg/kg) acquired a spatial navigation task more slowly than controls. When the escape platform was removed, the drug-treated rats did not preferentially search for it in the area where the platform had been during the acquisition phase. However, when the escape platform was visible, no differences in the performance of ketamine-treated and control rats could be found. In summary, ketamine seems to attenuate some but not all forms of learning in the tunnel maze and it impairs the acquisition of a spatial navigation task.

Animals↗
PubMed1988

The effects of long-term nicotine treatment on locomotion, exploration and memory in young and old rats.

To assess the effects of long-term treatment with nicotine on several behavioral measures (locomotor activity, exploratory efficiency, habituation, short-term and long-term memory) of young (5 months) and old (22 months) rats in a hexagonal tunnel maze, nicotine was added to the drinking water (0, 20 or 50 mg/l) for up to 131 experimental days. With the exception of effects on exploratory efficiency, young and old rats did not differ in their response to the drug. Nicotine decreased body weight throughout the experiment. Nicotine treatment reduced water intake during the first 30 min of the daily 4.5 h access to drinking water. Nicotine increased locomotor activity throughout the experiment. When nicotine treatment was discontinued during a 7-day withdrawal period, locomotor activity immediately dropped to control values. Intertrial habituation was not affected by nicotine. Long-term nicotine treatment had an attenuating effect on exploratory efficiency in young rats; however, the drug did not influence performance in tasks measuring spatial memory. Finally, age increased weight, decreased locomotor activity and impaired exploratory efficiency and short-term memory. Age, however, did not affect the performance of the long-term memory task.

Aging↗
PubMed1986

Unilateral intranigral injection of MPTP in the rat induces contraversive turning.

Unilateral injection of MPTP into the pars compacta of the substantia nigra in rats induced contraversive turning immediately after the injection. Contraversive turning decreased, and reversed its direction after about 30 min. Ipsiversive turning was still present 24 h after the injection of MPTP. These results suggest that MPTP has an initial stimulatory effect on dopaminergic neurons followed by a depression of the activity of these cells.

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine↗
PubMed1985

Intracranial application of substances in the unrestrained, awake rat by pressure injection through glass micropipettes.

A method is described which allows intracranial injection of drug solutions through glass micropipettes (tip diameter 15 micron) in the freely moving rat by use of air pressure. Compared with conventional injection methods through steel cannulae this method has the advantages of (1) minimal destruction of brain tissue, (2) precise injection of small volumes of solutions (1 nl to several microliters), (3) simultaneous recording of local EEG activity, and (4) usage of multipipette assemblies.

Animals↗
PubMed1984

Perioral biting reflex and turning after intranigral injection of a GABA- or metenkephalin-agonist: role of the thalamus and superior colliculus.

Unilateral destruction of the ventromedial thalamus (VMT) with a radiofrequency lesion attenuated turning induced by injection of muscimol (40 ng/0.4 microliter) but not of a metenkephalin-analogue (DAME; 2.5 micrograms/0.4 microliter) into the substantia nigra, pars reticulata (SNR). Unilateral lesions in the deep layers of the superior colliculus (DLSC) attenuated both muscimol- and DAME-induced turning. Lesions in the DLSC but not in the VMT blocked the sensitization of the perioral biting reflex by injection of muscimol or DAME into the SNR on the same side of the lesion. When injected with apomorphine (0.5 mg/kg) all rats with lesions turned ipsiversive to the lesion side and reacted to tactile stimulation of the perioral area on both sides with orienting towards and then biting into the stimulus probe.

Animals↗
PubMed1981

Sensitization of the perioral biting reflex by intranigral GABA agonist after detelencephalization.

Unilateral injection of 160 ng (0.4 microliter) of muscimol into the substantia nigra pars reticulata of rats elicits contraversive turning and simultaneously sensitizes the perioral biting reflex on the side of the face contralateral to the injected substantia nigra. Bilateral removal of the telencephalon eliminated neither the muscimol-induced behavior nor the peroral biting reflex.

Animals↗
PubMedDate not supplied

Facilitation of tunnel maze performance by systemic injection of the neurokinin substance P.

The effect of peripheral injections of substance P (SP) on performance in two different configurations of an automated tunnel maze was examined in three experiments. In two experiments, the effect of pretrial SP injections (10-1000 micrograms/kg) on performance in the hexagonal and radial maze configuration of an automated tunnel maze was investigated. In the hexagonal maze, which measures activity, exploratory efficiency, habituation, and perimeter walking, injection of SP affected perimeter walking only. In the radial maze, SP produced a facilitation of measures of efficiency and long-term and short-term memory without affecting activity. In the third experiment, the effect of pre- and posttrial injections of SP (50 or 500 micrograms/kg) on performance in the radial maze configuration was tested. Again, pretrial injections of 500 micrograms of SP facilitated performance with respect to measures of efficiency and short- and long-term memory; 50 micrograms produced a weaker effect. Virtually no effect was seen with posttrial injections.

Animals↗