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Biomedical subjects

H Weber

Publications and source records attributed to H Weber.

At least 469 records · Page 26Linked to original sources
PubMed1981

Homologous 3'-terminal regions of mRNAs for surface antigens of different antigenic variants of Trypanosoma brucei.

Sequences corresponding to the complete 3'-terminal regions of the messenger RNAs for three different Variant Surface Glycoproteins of Trypanosoma brucei were determined on complementary DNA inserts cloned in recombinant plasmids. The three sequences show 80-130 base pair long segments of strong (70-80%) homology at the 3' ends, whereas ther regions upstream from the last 130-140 base pairs contain no significant homology. The signal AAUAAA, present near the 3' ends of almost all known polyadenylated mRNAs of eukaryotes, does not occur in the 3'-terminal sequences of these three variants.

Amino Acid Sequence↗
PubMed1981

Microbiological implications of electric field effects. III. Stimulation of yeast protoplast fusion by electric field pulses.

Prototrophic colonies could be selected on minimal medium after mixing of protoplasts from diauxotrophic mutants of the yeasts Saccharomycopsis lipolytica and/or Lodderomyces elongisporus and treatment with polyethylene glycol (PEG) in the presence of calcium chloride. This is the result of protoplast fusion and complementation of auxotrophic deficiencies. Under identical conditions an electric field pulse in the mus-range applied via an electric discharge to the protoplast-PEG mixture resulted in a drastic enhancement of the protoplast fusion rate. The presence of polyethylene glycol was demonstrated to be a prerequisite for fusion in this case, too. The frequency of hybrid formation detected a prototrophic colonies could be increased in the case of intraspecific fusion at initial electric field strengths between 2.5 and 5 kV . cm-1. The application of an electric field pulse of proper strength and duration to a yeast protoplast suspension turned out to be a more effective tool in production of fusion products that conventional methods. Large numbers of parasexual hybrids for different selection programmes in yeast genetics and for industrial purpose may be delivered by this technique.

Electricity↗
PubMed1981

Fontan procedure--indication and clinical results.

Twenty-six patients with tricuspid atresia (15), univentricular heart (7), and single ventricle (4) underwent 27 Fontan or modified Fontan procedures between 1975 and 1981. The age of the patients varied between 4 and 26 years. Twenty patients had had a total of 33 palliative operations prior to correction. The original Fontan procedure was performed in 10 patients from 1975 to 1977. According to the various anatomical findings modifications of the Fontan procedure, such as direct anastomosis or implantation of a valveless conduit, were introduced in 1977. Early mortality among all the patients was 22% (6 patients died). Three deaths occurred in the initial period 1975 to 1977. Among the last 20 patients (1978 to 1981) there were 3 early deaths. Three patients with single ventricle survived, one died due to pulmonary failure. There were 2 late deaths (sepsis, sudden cardiac death). Postoperative cardiac catheterization performed in 17 patients revealed excellent results in 13 patients; the remaining 4 displayed diminished arterial oxygen saturation, three of them had Glenn palliation prior to corrective surgery. Postoperative right atrial mean pressure varied from 10 to 23 mmHg. The left ventricular parameters were within the normal range.

Adolescent↗
PubMed1981

[Bacterial endocarditis in congenital heart defects (author's transl)].

Seven patients with bacterial endocarditis have been treated at the Department of Pediatric Cardiology University Göttingen from September 1975 to July 1978. All of them had congenital heart defects considered as a predisposing condition and confirmed by previous cardiac catheterization, three patients were cyanotic. Unlike the spectrum of micro-organisms prevalent hitherto Pseudomonas aeruginosa was isolated in three cases. In one of these patients antibiotic drug therapy was unsuccessful and only surgical management with closure of the rest-VSD and excision of the endocardiac lesions was finally helpful. Another patient died and Pseudomonas aeruginosa endocarditis was disclosed by post mortem examination only. The onset of symptoms in four patients was less than six months after cardiac surgery. In two cases aortic valve prosthesis became necessary. No relapse during the further follow up period was observed.

Adolescent↗
PubMed1981

[Propafenon therapy of arrhythmias in infancy and childhood (author's transl)].

The therapeutic dose range of propafenon was studied in 28 infants and children aged 5 days to 15.2 years (mean 8.8 years) with the following cardiac arrhythmias: paroxysmal tachycardia (20) atrial flutter (5) ventricular extrasystoles (2) and junctional tachycardia (1). With the intravenous administration of the drug in a dosage of 1.03-3.2 mg/kg/d (mean 2.28 mg/kg/d) in patients less than 15 kg and of 0.71-2.06 mg/kg/d (mean 1.26 mg/kg/d) in patients more than 15 kg body weight arrhythmias abolished. In the same patients the oral dosage was 12.2-22.6 mg/kg/d (mean 16.03 mg/kg/d) and 7.2-16.6 (mean 11.12 mg/kg/d). After 10 to 14 days a therapeutic serum level above 150 ng/ml could be maintained by a reduced oral dosage of 5.7-13.3 mg/kg/d) mean 8.5 mg/kg/d) and 3.6-11.1 mg/kg/d (mean 6.33 mg/kg/d) respectively. There were no side effects except for transitory by elevated gamma-GT in 8 patients. During a follow-up period of 1 month to 2.2 years (mean 1.6 years) 20 patients had no more arrhythmias, in 7 the number of arrhythmic attacks was reduced, and in 1 therapy failed.

Adolescent↗
PubMed1981

[Computer-assisted long-term ECG analysis in patients with artificial trial pacemaker (AAI) (author's transl)].

Control of AAI-function is limited. To investigate the efficacy of AAI-stimulation, a computer-assisted analysis system for long-term ECG records was developed using "Multipass-Scanning I". 12 patients (5 females, 7 males, 33--78 years old with AAI) underwent Holter-monitoring for 24 hours continuously. THe ECGs of 5 among the collective with heart rate programmable AAI were recorded three times each with different AAI stimulation rates (50--80 b.p.m.). The number of AAI-induced heart beats in relation to the total number of QRS-complexes (AAI-QRS%) was 43 +/- 37% mean. AAI-QRS% during day showed a significantly lower distribution (35 +/- 38%, p less than 0.01) than during night (58 +/- 40%, p less than 0.001). Using an AAI-stimulation frequency of 50 b.p.m., only 9% of the heart beats were AAI-induced during day. Whereas AAI-QRS% increased to 72% during night in patients with AAI-stimulation frequency of 70 b.p.m. The interval between the PM-spike (S) and the following answer of the heart, i.e. SR-interval, demonstrated also a circadian behaviour: SR max occurred between 10 p.m. and 2 a.m. It was significantly prolonged to the SR min. (9--31%, m 20%, p less than 0.0001), which occurred between 9 a.m. and 4 p.m. So the presented computer-assisted long-term ECG analysis system for AAI-pacemakers is able to evaluate the efficacy of AAI with regard to different AAI-stimulation rates. The quantity of AAI-induced heart beats is dependent on patient's heart rate, its circadian behaviour and the stimulation rate of the AAI. The SR-interval also demonstrated a significantly circadian pattern, which can be related to the vagus-influenced PQ- and also AH-interval.

Adult↗