Biomedical subjects

H Wang

Publications and source records attributed to H Wang.

At least 541 records · Page 30Linked to original sources

PubMed2001

[Multivariate logistic regression analysis on the risk factors of type 2 diabetes mellitus].

OBJECTIVE: To explore the relationship between multivariate factors and type 2 diabetes mellitus. METHODS: Polymorphisms of microsatellite markers in uncoupling protein 3 (UCP3) gene, hormone-sensitive lipase (HSL) gene and protein tyrosine phosphatase-1B (PTP-1B) gene were tested in 106 patients with type 2 diabetes and 102 control subjects by performing polymerase chain reaction (PCR), polyacrylamide gel electrophoresis and silver staining. Multivariate logistic regression analysis was performed by all factors. RESULTS: Through univariate analysis, type 2 diabetes had significantly positive associations with age, systolic blood pressure (SBP), fasting insulin (FINS) level, cholesterol, triglyceride, low-density lipoprotein, apolipoprotein B (ApoB), alpha lipoprotein, UCP3 gene allele 6, UCP3 gene allele 7, HSL gene allele 9, and negative associations with UCP3 gene allele 1 and 3, HSL gene allele 5, high-density lipoprotein. The results of multivariate logistic analysis showed that UCP3 gene allele 6, UCP3 gene allele 7, SBP, ApoB, alpha lipoprotein were still positively related to type 2 diabetes, while HSL gene allele 5, high-density lipoprotein were still negatively related to type 2 diabetes. CONCLUSION: Our data showed that UCP3 gene allele 6, UCP3 gene allele 7, SBP, abnormity of plasma ApoB and alpha lipoprotein might play a role in the development of type 2 diabetes. HSL gene allele 5, high-density lipoprotein might play some protective role in the development of type 2 diabetes.

Blood Pressure↗

PubMed2001

[Preliminary study of HLA haplotype matched and T-cell undepleted allogeneic bone marrow transplantation for treatment of leukemia].

OBJECTIVE: To explore the feasibility of allogeneic bone marrow transplantation (Allo-BMT) with graft from HLA haplotype matched related donor without T-cell depleted for the treatment of leukemia. METHODS: Fifteen patients with leukemia received allo-BMT with grafts from HLA 2 or 3 antigen mismatched related donors. All patients were treated with standardized conditioning regimen consisting of high dose Ara-C, cyclophosphamide (CY) and total body irradiation (TBI). Donors were given G-CSF at 3 to 4 micrograms.kg-1.d-1 for seven days prior to marrow harvest. GVHD prophylaxis programme consisted of CsA, MTX, ATG and mycophenolate mofetial. RESULTS: All patients established successful engraftment. The median days of granulocyte > 0.5 x 10(9)/L and platelet > 20 x 10(9)/L were 19(range 13-23) and 21 (range 16-32) days, respectively. Acute grade II-IV GVHD occurred in 5 of 15 patients (33.3%). Two of them were grade II gut aGVHD, 2 grade III gut aGVHD, and 1 grade IV gut and liver aGVHD. Chronic GVHDs were seen in 8 of 9 evaluable patients (88.9%) and none developed extensive cGVHD. The median follow-up duration was 395 (110-690) days. Six of fifteen patients died. Five of them died from transplantation related mortality and 1 from relapse. Nine patients were alive in a disease-free situation. Six of them survived more than one year. CONCLUSION: The major histoincompatibility barriers in the haplotype matched related donor/recipient allo-BMT might be crossed by donor stimulated with G-CSF and combined GVHD prophylaxis program.

Adolescent↗

PubMed2001

Antioxidant properties of phenolic diterpenes from Rosmarinus officinalis.

AIM: To investigate the inhibition capacities of carnosol, rosmanol, and epirosmanol, which are phenolic diterpenes from Rosmarinus officinalis, to oxidized low-density lipoprotein (LDL) formation in human blood and detect their scavenging activities to lipid free radical and superoxide anion in vitro. METHODS: The antioxidant activities which were expressed with the inhibilities to lipid free radicals in the membrane lipid of cell and oxidized LDL formation were evaluated by TBARS assay and ESR method. The inhibition on the Cu2+-mediated oxidization of apo B formation in LDL was investigated by fluorescence spectroscopy. RESULTS: Carnosol, rosmanol, and epirosmanol had an inhibitory activity to lipid peroxidation and oxidized apo B formation in human bloods LDL. The IC50 were 7-10 micromol/L. The antioxidant mechanism was related to the scavenging activities to lipid free radical. CONCLUSION: carnosol, rosmanol, and epirosmanol showed the activity in inhibiting LDL oxidation.

Abietanes↗

PubMed2001

Inhibitory role of GDP on saikosaponin (I) stimulated enzymes secretion and rising of [Ca2+]i in rat pancreatic acini.

AIM: To study the role of guanosine diphosphate (GDP) on enzyme secretion and rising of [Ca2+]i in saikosaponin (I) [SA(I)] stimulated rat pancreatic acini. METHODS: Cell membrane of isolated rat pancreatic acini were permeabilized using streptolysin O (SLO). Enzymes secretions were indicated by detecting total protein secretions. Intracellular Ca2+ ([Ca2+]i) was measured using Fluo-3 in SPEX spectrofluorimeter. RESULTS: The inhibition of GDP on SA(I) stimulated enzymes secretion increased with increasing GDP concentration. There were two peaks in the time course of increase in [Ca2+]i evoked by SA(I) 10 micromol/L. After adding GDP 5 mmol/L, [Ca2+]i rose gradually without the two peaks. In permeabilized acini, the accumulation of enzymes stimulated by SA(I) in 30 min reduced by 57 % compared with intact acini. GDP 5 mmol/L decreased the initial rate of secretion. CONCLUSION: Inhibition of GDP on increase in [Ca2+]i reduces SA(I) stimulated enzymes secretion in pancreatic acini.

Animals↗

PubMed2001

[Effect of Zn2+, Cd2+ and their combined on Ca, Fe and Mn uptake by wheat seedlings].

The effect of Zn, Cd and their combination on Ca, Fe and Mn uptake by wheat seedlings was studied with solution culture. The result shows the Zn and Cd contents of wheat increased with increasing concentrations of Zn2+ and Cd2+ in solution, while there was a difference between single and compound treatments. Zn affected Cd uptake by wheat, and Cd inhibited Zn uptake. The uptake of Ca and Mn reduced with the increase of Zn2+ and Cd2+ in solution. The uptake of Fe increased with increasing Zn2+ and Cd2+ concentrations in single treatment, while decreased in compound treatment. The interactive effect of Zn and Cd was also related to the parts of plants.

Cadmium↗

PubMed2001

[Preliminary approach to environmental risk assessment of drainage projects during oilfield exploitation in Daqing].

A qualitative analysis was made to assess the environmental risk and its impact of alternative drainage projects around Yong-5 oil-transport station at Yongle oilfield in Daqing. On the basis of this, a method of fuzzy comprehensive assessment was applied to set up corresponding indexes, to determine hazard environmental coefficients, and to calculate the environmental risk fuzzy assessment indexes which reflect the relative risk degrees of alternative drainage projects. The measures on environmental risk management were proposed according to the priority of alternative projects.

China↗

PubMed2001

[Diagnosis and surgical treatment of peripheral intrahepatic cholangiocarcinoma].

OBJECTIVE: To summarize the experience in diagnosis and surgical treatment of peripheral intrahepatic cholangiocarcinoma (PICC). METHODS: Clinicopathological features, surgical treatment and prognosis of 20 patients with PICC treated in our hospital from 1970 to 1999 were retrospectively analyzed. RESULTS: The patients lacked typical clinical manifestations and some had a history of hepatitis B (35%), cirrhosis (45%), and elevation of serum alpha-fetaprotein (AFP) (25%). The diagnostic rates of ultrasound examination (BUS), CT, MRI and emission computerized tomography (ECT) were 90% (18/20), 94% (17/18), 100% (3/3) and 100% (4/4), respectively. However, the liver lesions detected by these methods needed to be differentiated from hepatocellular carcinoma (HCC), metastatic neoplasm and liver cyst, etc. In all the patients, the resection rate was 60% (12/20) and postoperative overall 1-, 3- and 5-year survival rates were 55% (11/20), 35% (7/12) and 20% (4/20), respectively. The liver resection rates were 83% (11/12), 58% (7/12) and 33% (4/12), respectively. Of the 4 patients who had survived for more than 5 years, 3 had a small liver carcinoma with a diameter less than or equal to 3 cm and one had large nodular liver carcinoma with intact capsule. After operation, all the patients received selective hepatic artery perfusion chemotherapy. CONCLUSION: PICC patients lack specific serum tumor marker can achieve good survival after early detection and curative resection with adjuvant therapies.

Adult↗

PubMed2001

[Human factor VIII expression in retrovirus vector-transduced bone marrow stromal cells].

OBJECTIVE: To explore the feasibility of bone marrow stromal cells (BMSCs) used for gene therapy of hemophilia A. METHODS: Murine and rabbit BMSCs were transduced with a recombinant replication-incompetent retrovirus LNC-FVIII delta B, which encloses a B-domain-deleted (760aa-1,639aa) human factor VIII cDNA. An optimized protocol involving temperature declining (32 degrees C) and centrifugation was used. Antigen and coagulant activity of human FVIII in the cell culture medium were measured by ELISA assay and one-stage method, respectively. Semi-quantitative PCR was performed for the transduction efficiencies. Western blot was used for FVIII protein analysis. RESULTS: Transduction efficiencies were increased 5- to 8-fold by temperature decline and centrifugation, which resulted in high expression and secretion of FVIII in transduced mouse[(556 +/- 80) ng/10(6) cells/24 hr] and rabbit [480 +/- 56) ng/10(6) cells/24 hr] BMSCs. Coagulant activities of human FVIII secreted from transduced murine and rabbit BMSCs were 2.42 U/10(6) cells/24 hr and 1.8 U/10(6) cells/24 hr, respectively. Western blot analysis demonstrated that the B domain-deleted FVIII cDNA was secreted predominantly as heavy and light chain heterodimers that resemble the native forms in human plasma. CONCLUSIONS: It was demonstrated that the BMSCs system has potential utility in the gene therapy for hemophilia A.

Animals↗

PubMed2001

[Clotting factor X deficiency resulted from an T 58-->G mutation within exon 1 of human factor X gene].

OBJECTIVE: To identify the genetic defect of a patient with clotting factor X deficiency (QGZ). METHODS: PCR and DNA sequencing were used to screen mutation in factor X gene. PCR primers were designed covering all the coding regions for exon and flanking intron sequences. RESULT: A single nucleotide substitution T 58G in exon 1, which caused a missense mutation Ser(AGT) 11 Arg(AGG) in signal peptide, was identified by DNA sequencing. CONCLUSION: This nucleotide substitution might be the molecular etiology of factor X deficiency.

Base Sequence↗

PubMed2001

[Clinical analysis of nosocomial infection of extended spectrum beta-lactamase-producing bacteria].

OBJECTIVE: To investigate the clinical characteristics of nosocomial infection of extended spectrum beta-lactamase(ESBL)- producing bacteria. METHODS: The data of 50 cases of ESBL(+) E. coli and K. pneumonia from January to November 1999 in Peking Union Medical Hospital were analysed; 45 cases of ESBL (-) infection were randomly selected as controls. t-test and chi-square tests were used for statistical analysis. RESULTS: Long time hospitalization and use of the third generation cephalosporins were risk factors for ESBL(+) pathogen infection(P < 0.02); the prevalence of ESBL(+) bacteria infection in abdominal and pelvic cavity was higher than that of ESBL(-) bacteria(P < 0.02); the outcome of properly treated group(sensitive antibiotics were used within 72 hours) was much better than improperly treated group. All ESBL(+) bacteria were sensitive to imipenam, the resistance rate of ESBL(+) bacteria to cefmetazole, amikacin and piperacillin/tazobactam was low; the in vitro activity of ceftazidim to ESBL(+) bacteria was high but the in vivo activity is still under study. CONCLUSION: The mortality of ESBL(+) bacteria infection was high; the outcome would be improved if sensitive antibiotics were chosen as soon as the diagnosis was made.

Anti-Bacterial Agents↗

PubMed2001

[A study on affecting factors on dental care demands by logistic regression model].

OBJECTIVE: To develop probability model for dental visits based on analysis of the factors affecting people's dental services utilization in urban area of Beijing, thus providing some evidence for forecasting further demanded dental care and building the more efficient oral health care delivery system. METHODS: A cross-sectional survey was conducted amnog 1,517 subjects of all age groups in Beijing selected by stratified, clustering, random sampling. The first model of dental care demand--probability model for dental visits was established with logistic regression. RESULTS: Awareness of oral health showed the most important relationship. Different social background and economic factors also had great effects on demand. Higher demand for care were existed among those who suffered from a disease that was painful or resulted in poor oral function. CONCLUSIONS: It is suggested that dental care has different characters from general health service. Utilization of dental service is largely determined by people's awareness, income and insurance system. So oral health education should be extended in order to promote the effective demands and reduce the potential demands. Rational oral health insurance system should be set up to provide some priority to certain people, thus stimulating the supply of dental care and improving the utilization of dental service. Since raising price for care will restrain the low-incomer's basic demand, and it will neither add the profit nor improve people's oral health, the price decision should be considered carefully. At last, oral health care should be emphasized on children and adolescent as priority age groups based on the conclusion from demand model.

Dental Care↗

PubMed2001

[Cloning and biological regulating effects of human WAF1 gene].

OBJECTIVE: To clone human WAF1 gene and construct WAF1-pcDNA3 recombinant plasmid, and to explore the biological regulating effects of WAF1 gene. METHODS: Human WAF1 gene was amplified and cloned into pcDNA3 vector by RT-PCR and DNA direct sequencing from Hela cells. By immunofluorescence and immunohistochemistry staining, the identify the expression of the inserted WAF1 gene and its biological regulating effects on Rb, cyclin D1 gene. RESULTS: Human WAF1-pcDNA3 recombinant plasmid was correctly constructed and transfected into Hct-8 cells. P21WAFI/Clip was successfully expressed. The functional characterization of the inserting WAF1 gene implied that P21WAF1/Cip1 expression could upregulate Rb gene and downregulate cyclin D1 gene at protein level. CONCLUSION: Human WAF1-pcDNA3 can modulate the protein expression of the downstream gene.

Cloning, Molecular↗

PubMed2001

[The Rsa I and Sma I polymorphisms in the von Willebrand factor gene discrimination by hybridization with oligonucleotide arrays].

OBJECTIVE: To develop a new assay for the Rsa I and Sma I polymorphisms (single nucleotide polymorphism, SNP) in the von Willebrand factor gene detection that utilizes oligonucleotide arrays (DNA chips) on glass supports. Expoloring the association of the RsaI and SmaI polymorphisms in the von Willebrand factor gene with thrombosis in Chinese. METHODS: Allele-specific oligonucleotides are covalently immobilized on glutaradehyde derivatized glass slides in arrays. Single strand PCR product of PCR-amplified genomic DNA is fluorescently labeled by assymetric PCR with fluorescently tagged dUTP and hybridized to the support-bound oligonucleotide array. The hybridization pattern is detected by fluorescence scenning. The effect of hybridization conditions were evaluated and optimized. The method was validated by the discrimination of blinded DNA samples identified by restriction enzymes. 50 patients with thrombosis were examined by this method. RESULTS: The genotypes scored by oligonucleotide arrays assay were in 100% agreement with restriction enzymes results. The genotype distributions GG, GA, AA and CC, CT, TT of RsaI and SmaI polymorphisms of patients or controls were 4.0%, 12.0%, 84.0% and 24.0%, 44.0%, 32.0% or 1.4%, 11.8%, 86.8% and 8.8%, 57.4%, 33.8%. The allele frequencies G, A and C, T were 10.0%, 90.0% and 46.0%, 54.0% or 7.4%, 92.6% and 37.5%, 62.5%. No statistical difference in genotype distribution and allele frequencies was observed between patients with thrombosis and the control groups (all P > 0.05). CONCLUSION: A rapid and accurate method for the RsaI and SmaI polymorphisms in the von Willebrand factor gene detection, or for the analysis of single nucleotide polymorphism (SNP) has been developed using oligonucleotide arrays on glass supports. No association was observed between the Rsa I and Sma I polymorphisms in the vWF gene and thrombotic disease.

Alleles↗

PubMed2001

[Cloning and analyzing of the conserved protein gene in Streptococcus sanguinis ATCC10556].

OBJECTIVE: To clone and analyze the conserved protein gene in Streptococcus sanguinis(S.s) ATCC10556. METHODS: Southern blotting hybridization, molecular cloning techniques and dot blotting hybridization were employed to obtain the target clone which harbored the specific DNA fragment. The nucleotide sequence of the target clone was determined. The gene and its product were analyzed by Genework software and bioinformatics techniques. RESULTS: The conserved protein gene in Streptococcus sanguinis ATCC10556 was cloned for the first time and named as Streptococcus sanguinis conserved protein gene (Sscp gene). The Genbank accession number for Sscp gene is AY032739. The conserved protein gene in S.s ATCC10556 (named sscp163) was a transmembrane protein and contained two AAA domains. CONCLUSION: sscp163 protein might take part in the cell division in Streptococcus sanguinis; its function is similar to that of FtsK in E.coli.

Amino Acid Sequence↗

PubMed2001

[The transfer and expression of human clotting factor IX in muscle mediated by electroporation].

OBJECTIVE: To probe into the feasibility of increasing hFIX cDNA transfer and expression in muscle. METHODS: The high-frequency electric field was used to promote both Lac-Z-encoding plasmid pCMV beta and hFIX-expressing plasmid G1NaMCIX to transfer and express in muscle. The effects of frequency and length of square pulse, as well as eletroporation time on hFIX expression were investigated. RESULTS: Electric stimulation could increase the transfer and expression of pCMV beta in muscle, the number of X-gal positive myofiber cells in electroporation-treated mice is 2.1 times larger than that of mice not treated by electroporation (P<0.01). The most optimal electric simulation condition for hFIX cDNA transfer and expression was obtained, under this condition, the highest level of hFIX antigen in plasma is (40+/- 5.4) ng/ml and 7 times higher than that of mice without electroporation P < 0.001). CONCLUSION: Electroporation is able to enhance hFIX cDNA transfer and expression in muscle efficiently.

Animals↗

PubMed2001

Effect of all-trans retinoic acid and arsenic trioxide on tissue factor expression in acute promyelocytic leukemia cells.

OBJECTIVE: To study the effect of all-trans retinoic acid (ATRA) and arsenic troxide (As2O3) on tissue factor (TF) expression and procoagulant activity (PCA) of acute promyelocytic leukemia (APL) cells in vivo and in vitro. METHODS: PCA from freshly isolated APL blasts from APL patients treated with ATRA or As2O3 was detected using a one-stage clotting assay. TF antigen was detected by ELISA and TF mRNA by RT-PCR. The maturation sensitive (NB4) or resistant subclones (NB4-R1) of the promyelocytic NB4 cell line, as well as U937 cells infected with pMSCV-PML-RARa treated with or without ATRA or As2O3, were also examined. RESULTS: Both ATRA and As2O3 can down-regulate the TF antigen, its mRNA transcription and membrane PCA of APL cells in vivo and in vitro, in a time-dependent manner. The TF antigen level in PML-RARa + U937 cells was significantly higher than that in U937 cells infected with retrovirus vector. Both ATRA and As2O3 can also down-regulate the TF antigen in U937 cells transfected with or without PML-RARa. CONCLUSION: Tissue factor expression and PCA in APL cells may be down-regulated by ATRA and As2O3. By down-regulating TF expression, As2O3 might also be used to improve the DIC-related hemorrhage in APL. Our data indicate that elevated TF antigen in PML-RARa + U937 may be related to the fusion protein PML-RARa. The down-regulating effect of ATRA and As2O3 on TF expression in U937 cells might not involve this fusion protein.

Adolescent↗

PubMed2001

Astragali and Angelica protect the kidney against ischemia and reperfusion injury and accelerate recovery.

OBJECTIVE: To investigate the role of Astragali and Angelica (A&A) of Chinese herbs in acute renal ischemia/reperfusion injury, and the related intracellular signal transduction mechanism. METHODS: Acute ischemic renal injury in rats was induced by clamping in renal pedicel for 45 minutes. Rats in therapy group were given a single dose (2 ml/day) of A&A for 3 days before clamping, and then continued for another 3 days. Forty-five minutes after clamping and at different reperfusion time, serum creatinine (Scr) and renal pathological changes were taken and compared in both groups. The proliferating cell nuclear antigen (PCNA) positive cells were detected by immunohistochemistry. Extracelluar regulating kinase (ERK) and c-Jun N-terminal kinase (JNK) activity was assayed by specific substrate phosphorylation with immunoprecipitation. RESULTS: At the 24th hour of reperfusion, Scr was lower in A&A group than that in the control. Much less necrotic tubular cells, casts, and more PCNA-positive cells were found in A&A group. ERK activity decreased after clamping, and recovered at 5 minutes of reperfusion. There was no difference between the two groups. JNK activity did not change after ischemia, but increased at 5 minutes and peaked at 20 minutes of reperfusion. JNK activity was significantly higher in A&A group than that in the control group. CONCLUSION: A&A protected kidney against ischemic insult and accelerated both functional and histological recovery after acute renal ischemia/reperfusion injury, which may associate with the change of JNK signaling pathway.

Angelica↗

PubMed2001

Low incidence of severe aGVHD and accelerating hemopoietic reconstitution in allo-BMT using lenograstim stimulated BM cells.

OBJECTIVES: To investigate the efficacy of accelerating hemopoietic reconstraction and reducing a graft versus host disease (GVHD) in Allo-BMT receiving lenograstim stimulated donor marrow and to assess the preliminary biological mechanism. METHODS: The donors for thirty patients (study group) with leukemia were given lenograstim 3-4 micrograms.kg-1.d-1 for seven days prior to marrow harvest. The results of subsequent engraftment in the recipients was compared with fifteen donors without G-CSF (control group). Five donors themselves were studied to assess the effects of lenograstion on hematopoietic progenitor cells and lymphocyte subsets in BM. RESULTS: The stimulated bone marrow contained a higher number of nucleated cells, CFU-GM and CD34+ cells (P < 0.01). The hematopoetic reconstitution was accelerated. Until granulocyte counts exceeded 0.5 x 10(9)/L and plalete counts exceeded 20 x 10(9)/L, the days were 16.7 +/- 3.2 and 18.4 +/- 3.0 days as compared with those of the control group (22.5 +/- 5.1 and 26.3 +/- 5.9 days respectively, P < 0.01). The incidence of grade II-IV aGVHD was very low, only one case with grade II aGVHD on the skin in the study group. Four out of fifteen patients (26.7%) in the control group had grade II-IV aGVHD (P < 0.05). The number of T lymphocyte subsets in the harvested BM stimulated by G-CSF changed. In comparison with the control group, CD4+ decreased and CD8+ increased significantly (P < 0.01). The changes of progenitor cells and T lymphocyte subsets in BM from pre- to post-G-CSF stimulation indicated that the percentage of CD4+ cells reduced (P < 0.05), that of CD8+ cells, and that of CD34+ increased (P < 0.01). The incidence of chronic GVHD and relapse of leukemia were not different significantly between both groups. CONCLUSIONS: Allogenic bone marrow transplant (Allo-BMT) donors given G-CSF can accelerate engraftment and minimize the incidence of severe aGVHD. There is a trend in favour of improved transplant-related complications.

Acute Disease↗