One of the aims of the facility is to develop and push the automated on-line DNA sequencing gel technology to its limit in sequence throughput, which may be somewhere around 100 kilobases of sequence per device per day. Key new developments were initiated and applied in operation on the European Molecular Biology Laboratory (EMBL) automated sequencer and its commercial version A.L.F. (Pharmacia). Sequencing speed was increased by a factor of 10-20, up to 1500 bases per hour per clone on ultrathin (about 100 microns) gels, while the resolution and reading length were extended to 1000 bases on gels with 50 cm separation length, using fluorescein-15-*dATP as the internal label. With our sequencing strategy, closing about 40% of the sequence with "walking" primers and F-15-*dATP as internal label, we sequenced both strands of a cosmid insert of 38.5 kb in length, each strand twice, in only 430 sequencing reactions and with average reading of 380 bases per reaction.

Base Sequence↗

PubMedDate not supplied

[No motivation, no time, no money--does oncologic graduate and continuing education still have a future? Presentation at the 50th Scientific Session of the Society of Pediatric Oncology and Hematology within the scope of the KOK and PSAPOH general session].

For many years the nursing profession has been considered a dead-end street without any prospects of career advancement or continued qualification. This situation has thoroughly changed. Nursing as an academic specialty has been established in 24 colleges. Furthermore, on-the-job specialty training has become available. In some regions of Germany the installation of specialty training courses has, however, been hampered by political opposition. The Northrhine-Westphalian situation is described to illustrate the reasons for such an attitude. At the same time many nurses lack motivation to continue on-the-job training. Benefits (such as advancement opportunities) for those who complete training might serve to increase the motivation and participation of nurses. Specialty training is essential in ensuring the quality of care particularly in high pressure fields such as oncology and also extends the nurses' personal range of experience, reasons enough to enforce the legal realisation of specialty training.

Child↗

PubMedDate not supplied

[The importance of the substrate for the induction of tyrosine-alpha-ketoglutarate-transaminase in RLC-cells (author's transl)].

1. Dexamethasone phosphate causes approximately a threefold increase of the tyrosine-alpha-ketoglutarate transaminase in the culture of RLC cells. 2. The induction of the enzyme depends on the presence of L-tyrosine. Omission of L-leucine or L-tryptophan, respectively, has no effect. 3. Omission of L-tyrosine influences the activity of the lactate dehydrogenase and malate dehydrogenase not at all and that of the glucose-6-phosphate-dehydrogenase only to a small extent. 4. In the absense of L-tyrosine an superinduction takes also place by actinomycin.

Cell Line↗

PubMedDate not supplied

[Hypersensitivity in schistosomiasis mansoni].

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Adolescent↗