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Biomedical subjects

H Ueno

Publications and source records attributed to H Ueno.

At least 361 records · Page 20Linked to original sources
PubMed1994

Myelodysplastic syndrome with t(9;11)(p22;q23) after treatment for B-cell acute lymphoblastic leukemia without epipodophyllotoxins.

Secondary myelodysplastic syndrome (MDS) with t(9;11)(p22;q23) developed in a child after intensive treatment for B-cell acute lymphoblastic leukemia diagnosed 12 months earlier. His chemotherapy had consisted mainly of cyclophosphamide and methotrexate, but no epipodophyllotoxins. The combination of MDS, the cytogenetic anomaly involving bands at 11q23 and no previous exposure to epipodophyllotoxins represents a unique case of therapy-related leukemia.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1994

Diagnostic value of captopril test in hypertensive patients with renal artery stenosis.

To examine the utility of the single-dose captopril test in detecting renovascular hypertension (RVHT), the authors measured peripheral plasma renin activity (PRA), before and thirty and sixty minutes after an oral dose of captopril (25 mg), in 28 patients with RVHT and 22 patients with high-renin essential hypertension (EHT) without renal artery stenosis who were consuming 8 grams of sodium chloride per day. There was considerable overlap of individual values in basal PRA between the two groups. Sixty minutes after captopril, PRA was higher in RVHT than in EHT patients (74.8 +/- 63.9 versus 15.1 +/- 11.9 ng/mL/hr, P < 0.01). With the cutoff point set at 16 ng/mL/hr, RVHT was detected with a sensitivity of 96% and a specificity of 77%. The discriminating power was also superior to that based on blood pressure response to angiotensin II analogue under sodium depletion, rapid-sequence intravenous pyelography, or renography. These results show that captopril-stimulated peripheral PRA is an adequate screening tool for detecting RVHT in a population with high-renin hypertension.

Adult↗
PubMed1994

Alterations of specific and nonspecific binding of monoclonal antibody by introduction of acidic and hydrophobic groups.

AB-6 monoclonal antibody (mAb) against bovine serum albumin (BSA) was coupled with beta-alanine or n-propylamine using a heterobifunctional cross-linking reagent, N-(epsilon-maleimidocaproyloxy)succinimide (EMCS) and a linker, poly-L-cysteine to introduce acidic or hydrophobic groups into the mAb. The mAb molecules coupled with beta-alanine at lower than 1:16 molar ratios and those coupled with n-propylamine at 1:14 retained the original reactivity to BSA but modification with higher amounts of beta-alanine or n-propylamine decreased the reactivity. In contrast, nonspecific bindings of AB-6 mAb to a plastic culture plate and tumor (MDA-MB-453) cells were decreased by modification with beta-alanine and increased by n-propylamine. These results suggested that introduction of acidic groups, and hydrophobic groups to antibody leads to fluctuations in the non-specific binding of antibody.

Animals↗
PubMed1994

Derivation and application of monoclonal antibodies recognizing several epitopes on bovine serum albumin.

Three (AB-3, AB-4 and AB-6) monoclonal antibodies (mAb) to bovine serum albumin (BSA) were derived and characterized for their physicochemical and immunological properties. AB-3 recognized an epitope distinct from epitopes recognized by AB-4 and AB-6 as determined by binding inhibition assay. AB-4 and AB-6 mAbs recognized similar but not identical epitopes on BSA. Based on the antigenic specificity, we applied these mAbs to quantitative analysis of BSA in medium and to depletion of BSA from culture medium containing fetal calf serum (FCS). For quantitative analysis, we employed a sandwich enzyme-linked immunosorbent assay (ELISA) using biotinylated AB-3, solid-phase of AB-6 and an avidin-biotin-peroxidase complex system. This assay was highly sensitive and quantitative in the range of BSA concentration at 10 to 1,500 ng/ml. To deplete BSA from medium, we prepared affinity-gel coupled to AB-6. Repeated treatment of FCS-containing medium with the affinity-gel efficiently depleted BSA from the medium. The depletion capacity was 0.74 to 1.0 moles of BSA/mole of coupled mAb.

Animals↗
PubMed1994

"Undifferentiated" cerebral primitive neuroectodermal tumor in a young adult--case report.

A 21-year-old female presented with a cerebral primitive neuroectodermal tumor (PNET) without either glial or neuronal differentiation, who has survived for 27 months without neurological deficit following total removal of the tumor, radiotherapy, and intensive chemotherapy. Both immunohistochemical and ultrastructural studies are important for the diagnosis of PNET. "Undifferentiated" PNET may be a distinct entity from common PNET which shows either glial or neuronal differentiation or both.

Adult↗
PubMed1994

[HTLV-I and retinal antigen recognized by T cells].

MT-2 immune but not naive murine (B10.BR) spleen cells responded not only to HTLV-I-infected cell lines but also to retinal antigens of various species by antigen-induced cell proliferation. The phenotype of the responding cells against HTLV-I-infected cell lines as well as retinal antigens was Thy-1.2+, CD4+ and CD8-. The antigen-induced cell proliferation against both HTLV-I-infected cell lines and retinal antigens was clearly inhibited by monoclonal antibodies to CD3, CD4, and MHC class II (I-AK). These data indicate that an epitope of HTLV-I-infected cell lines recognized by the CD4+ T cell is crossreactive to that of retinal antigens.

Animals↗
PubMed1994

[Exercise-nitroglycerine technetium-99m-2-methoxy isobutyl isonitrile tomoscintigraphic imaging for identifying diseased coronary vessels: comparison with thallium-201 standard exercise-redistribution study].

A same-day double injection protocol employing 99mTc-methoxy isobutyl isonitrile (MIBI) and myocardial single-photon emission computed tomography (SPECT) for detecting coronary heart disease (CAD) was assessed in 21 patients. Our exercise-nitroglycerin (NTG) MIBI study was performed as follows: 150 MBq 99mTc-MIBI was injected at peak exercise, and after 5 minutes 0.3 mg of NTG was sublingually administered. Then, SPECT was performed 1 hour later. Immediately after the 1st imaging, patients were injected of 750 MBq 99mTc-MIBI and were reimaged 1 hour later. Within 1 month, all patients were underwent standard exercise redistribution SPECT thallium (Tl) study. Of the 126 myocardial segments evaluated, 81 were judged as normal by both techniques, while the presence of stress defects were demonstrated in 37 segments (Agreement: 94%). Vessel sensitivities were 75% by MIBI and 67% by Tl. Specificities were 90% by MIBI and 93% by Tl. For the pattern of reversibility in myocardial segments with stress defects, the agreement was 73%. In conclusion, our exercise-NTG MIBI may be safely performed, giving results equivalent to those of standard stress-redistribution thallium studies.

Angina Pectoris↗
PubMed1994

[Myocardial SPECT with iodine-123-labeled beta-methyl-branched fatty acid in patients with angina pectoris].

To evaluate the usefulness of 123I-BMIPP as a tracer of fatty acid metabolism in patients with angina pectoris, we performed rest BMIPP myocardial SPECT and stress 201Tl SPECT in 20 patients with angina pectoris. BMIPP SPECT was evaluated in the defected regions with Tl redistribution. Abnormal findings in BMIPP were observed in 11 of 20 patients and in 14 of 29 myocardial segments with Tl redistribution. Such decreased uptake was observed more often in patients with multivessel disease (64% vs. 12%). In addition, the decreased BMIPP uptake was seen more often in the segments exhibiting hypokinesis than the segments showing with normal wall motion. Thus, BMIPP imaging may be available to detect myocardial ischemia, particularly in patients with severe coronary disease.

Aged↗
PubMed1994

[Pure red cell aplasia with thymoma, myasthenia gravis and normal pressure hydrocephalus].

Cases of pure red cell aplasia with thymoma and myasthenia gravis are rare. We described a patient who had concomitant pure red cell aplasia, thymoma, myasthenia gravis and a normal pressure hydrocephalus. A 63-year-old man with disturbances of gait, left blepharoptosis and anemia was presented to our hospital. Laboratory examination on admission revealed severe anemia. Bone marrow aspirates showed erythroid hypoplasia of marked degree. Chest x-ray and chest CT revealed a tumor to the right of cardiac wall. The tensilon chloride test and antiacetylcholine receptor antibody were positive. A hydrocephalus was demonstrated with brain CT. Cerebrospinal fluid pressure was in the normal range. He was diagnosed as having pure red cell aplasia with thymoma, myasthenia gravis and a normal pressure hydrocephalus. This appears to be a fairly rare case. It seems important to consider that a normal pressure hydrocephalus may have immunological disorders.

Humans↗
PubMed1994

[Dielectric behavior of the rabbit cornea as a measure of the healing process in injured epithelium].

To correlate changes in the passive electrical properties of the rabbit cornea with quantitative grading of corneal injuries induced by topical application of 70% ethanol, we measured ocular tissue impedances using a surface electrode over the range of 10(4)-10(8) Hz and followed their temporal changes for up to 17 days. Dielectric measurements on control eyes yielded a broad dispersion curve, which, in loss tangent terms, could be decomposed into two components: dispersion 1 on the low-frequency side and dispersion 2 on the high-frequency side. By defining the peak value of the total dispersion as Pt and those of subdispersions 1 and 2 as P1 and P2, the ratios, P1/Pt and P2/Pt, were found to serve as useful indices. Upon appearance of corneal erosion due to ethanol, P1/Pt markedly increased and returned to the control level with re-epithelialization of the cornea, and the time course of P2/Pt showed a mirror image to that of P1/Pt. Both ratios correlated well with the erosion area determined photographically. These results indicate that dielectric spectroscopy is applicable to the assessment of the extent and severity of corneal injury.

Animals↗
PubMed1994

Etiology of prune belly syndrome: evidence of megalocystic origin in an early fetus.

BACKGROUND: Prune belly syndrome is a rare and complicated condition affecting the genitourinary organs and abdominal wall. The etiology of abdominal musculature deficiency in prune belly syndrome is controversial. We present a case that should elucidate the etiology of this syndrome. CASE: A spontaneously aborted fetus at 12 weeks' gestation with an early stage of prune belly syndrome was investigated by necropsy and light and electron microscopy. Megalocystis resulting from urethral atresia was diagnosed. There was no hydroureter or hydronephrosis, and both light and electron microscopy demonstrated evidence of development of the abdominal musculature. Both testes were elevated as a result of the megalocystis. CONCLUSION: These findings suggest that hypoplasia of the abdominal musculature and cryptorchidism might develop secondary to the presence of chronic megalocystis in this syndrome.

Abdominal Muscles↗
PubMed1993

Dominant-negative mutations of platelet-derived growth factor (PDGF) receptors. Inhibition of receptor function by ligand-dependent formation of heterodimers between PDGF alpha- and beta-receptors.

We showed previously that a truncated form of the platelet-derived growth factor (PDGF) beta-receptor lacking its kinase region can form a nonfunctional heterodimer with the wild-type beta-receptor and thereby inhibit its signal transduction. In this paper we investigated whether the truncated form of either alpha- or beta-receptor could block the function of the other type of wild-type PDGF receptor. When the truncated alpha-receptor was expressed in Xenopus oocytes in excess over either the wild-type alpha- or beta-receptor, the Ca2+ mobilization signal elicited by either the wild-type alpha- or beta-receptor was completely blocked. The truncated beta-receptor abolished signaling by the wild-type alpha-receptor in response to PDGF-AB or -BB. However signal transduction by the alpha-receptor in response to PDGF-AA was not affected by the truncated beta-receptor. In the presence of PDGF-AB or -BB, both the wild-type and truncated beta-receptors formed a heterologous complex with the alpha-receptor in intact cells. A kinase-inactive beta-receptor (an ATP-binding site mutation) became cross-phosphorylated on tyrosine residues by the co-expressed wild-type alpha-receptor in response to PDGF-AB or -BB but not in response to PDGF-AA. These findings indicate that the alpha-and beta-receptors interact in response to PDGF-AB or -BB and are consistent with the hypothesis that the truncated alpha-receptor inhibits function of the wild-type beta-receptor through formation of a ligand-dependent nonfunctional alpha beta-heterodimer. A similar heterodimer can form between the truncated beta-receptor and the wild-type alpha-receptor. These observations provide useful information for future studies using dominant-negative mutations of PDGF receptors to selectively inhibit the actions of specific PDGFs in animals.

Animals↗
PubMed1993

Efficient immunostaining of tissue sections with chemically modified monoclonal antibody.

Monoclonal antibodies (mAbs) were modified with a photo-cross linker, sulfosuccinimidyl 2-(m-azido-o-nitrobenzamido)-ethyl-1,3'-dithiopropionate (SAND), and their efficacy in immunohistochemical staining of tissue sections was examined comparatively with that of unmodified mAbs. mAbs used were HBJ127 and SV2-61 gamma which recognized a proliferation-associated human gp125 cell surface antigen and a human c-erbB-2 protooncogene product, respectively. Both mAbs could stain relevant cancer tissue in frozen sections but not that in paraffin sections. Whereas SAND-modified mAbs intensely stained the cancer tissue in paraffin sections too, and the UV irradiation to SAND-modified mAb-treated tissue sections further increased the intensity of the staining. Scatchard plot analysis using 125I-labeled mAbs indicated that the augmentation of staining capacity by the SAND modification is due to the increase of the mAb amount capable of binding to the antigen.

Animals↗