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Biomedical subjects

H Ueki

Publications and source records attributed to H Ueki.

At least 145 records · Page 8Linked to original sources
PubMed1986

Distribution of 2,4-dinitrophenyl groups on the epidermal Langerhans cells of guinea pigs following skin painting with 2,4-dinitrochlorobenzene.

It has been postulated that epidermal Langerhans cells (LC) are essential for induction and elicitation of contact sensitivity (CS) to simple chemical allergens. Although the precise role of LC in CS has not been determined, recent reports have focused on a function for LC in the presentation of antigen to T lymphocytes. To investigate this possibility, the distribution of allergen on the epidermal LC of inbred strain 13 guinea pigs was observed by the immunofluorescent method using antibodies against Ia antigen and hapten. 2,4-Dinitrophenyl groups were found to be localized on/in Ia-positive epidermal LC of the animals following skin painting with 2,4-dinitrochlorobenzene. This finding is discussed in relation to the role of LC as antigen presenting cells in CS.

2,4-Dinitrophenol↗
PubMed1986

[Stewart-Bluefarb syndrome (kaposiform arteriovenous fistula with bone changes)].

In a 14-year-old female patient with arteriovenous shunts (Stewart-Bluefarb syndrome), fist-sized cutaneous swelling, venous dilatation, blue-brown pigmentation and painful subcutaneous fingertip-sized nodules were noted on the right lower thigh. In addition to an arteriovenous malformation, bone hypertrophy, bone atrophy and after bone defects were observed in the involved area on X-ray examination. Histological examination revealed increased vascularity and vascular cavernous structures with endothelial swelling and extravasation of erythrocytes, but no malignant changes.

Adolescent↗
PubMed1985

Simple and rapid identification of phosphorylated peptides from bovine brain myelin basic protein by reversed-phase high-performance liquid chromatography.

The phosphorylation sites of the myelin basic protein from bovine brain were determined after phosphorylation with a cyclic 3':5'-phosphate-dependent protein kinase from the same source. Three phosphorylated peptides were selectively and rapidly separated, before and after dephosphorylation, by reversed-phase high-performance liquid chromatography on a styrene 250 column under alkaline conditions. Partial sequencing of the peptides by automated Edman degradation revealed that the serine-115 residue located in the main encephalitogenic determinant of the protein was a phosphorylation site, in addition to the two phosphorylation sites established (threonine-34 and serine-55).

Amino Acid Sequence↗
PubMed1985

Fibronectin. Localization in normal human skin, granulation tissue, hypertrophic scar, mature scar, progressive systemic sclerotic skin, and other fibrosing dermatoses.

The histologic localization of fibronectin (FN) in normal human skin, granulation tissue, hypertrophic scar, mature scar, progressive systemic sclerotic skin, and tissue of other fibrotic disorders was investigated by an indirect immunofluorescence technique using specific antiserum prepared in rabbits against purified human plasma FN. In granulation tissue that developed just after traumatic wounding, FN seemed to increase remarkably in the wound as a fibrillar network. In the hypertrophic scar, one to five years after wounding, FN was detected in a linear or curling arrangement throughout the dermis. On the contrary, FN gradually decreased in the wound of the mature scar five to 20 years after wounding. There were some interesting observations among other diseases. In the skin of patients with progressive systemic sclerosis and morphea, FN was found to be localized faintly on the dermoepidermal junction and papillary dermis. In the involved skin of dermatofibroma, FN was observed in a curling arrangement throughout the dermis.

Adolescent↗
PubMed1985

Effects of histamine on collagen synthesis by cultured fibroblasts derived from guinea pig skin.

Fibroblast-like cells derived from guinea-pig skin were cultured for 3 h in the presence of various concentrations of histamine. The total protein synthesized was determined by the incorporation of radioactive proline, and the collagenous protein synthesized was measured by the incorporation of labeled hydroxyproline in the cell layer and medium. Synthesis of both total and collagenous protein increased in the presence of histamine in the concentration range of 10(1)-10(3) micrograms/ml. The ratio of collagen to total protein synthesized also increased at these concentrations. However, in no case was an increase found when H1 antagonist (chlorpheniramine) and H2 antagonist (cimetidine) were added with the histamine. DNA synthesis was not affected by histamine at the concentrations used. These results suggest that histamine increases the synthesis of collagen by fibroblast-like cells through H1 and H2-receptors.

Animals↗
PubMed1985

The distribution of 2,4-dinitrophenyl groups in guinea pigs following feeding of 2,4-dinitrochlorobenzene.

An immunofluorescent technique using anti-DNP antibody was employed to investigate the distribution of 2,4-dinitrophenyl (DNP) groups in various tissues following the feeding of 125 mg/kg 2,4-dinitrochlorobenzene (DNCB)-ethanol to guinea pigs that had been starved for 1 day. DNP groups were detected in the areas corresponding to the cytoplasm and the cell membrane of the epithelium in the upper gastrointestinal tract as well as on the cells of mesenteric lymph nodes, Payer's patches, the spleen, and peripheral blood. These results are discussed in relation to the mechanism of tolerance induction produced by the feeding of haptens.

Animals↗
PubMed1985

The suppressive effect of tape-stripping treatment of guinea-pig skin on the induction of contact sensitivity by intradermal injection of haptenated epidermal cells.

Contact sensitivity (CS) to 2,4-dinitrochlorobenzene (DNCB) was produced in inbred JY1-strain guinea pigs by the intradermal injection of epidermal cells (ECs) prepared from DNCB-painted skin (DNP-ECs). When the site of DNP-EC-induced CS was pretreated by tape stripping, the rate and intensity of the challenge reactions to DNCB were diminished. The ability of DNP-ECs to induce CS returned to normal when normal peritoneal macrophages together with DNP-ECs were administered into the stripped skin. Normal ECs had a similar effect. Using either anti-Ia antiserum and complement or allogeneic ECs (strains 2 and 13), Ia-positive cells among the ECs (presumably Langerhans cells) were found to be essential for the recovery of CS. Tape-stripping treatment also resulted in the development of immunological tolerance, as assessed by subsequent painting with a sensitizing dose of DNCB. These findings suggest that the immunological function of the mononuclear-phagocyte system in the dermis may be impaired when the epidermal surface is markedly disturbed by tape-stripping treatment.

Adenosine Triphosphatases↗
PubMed1985

T-cell subsets in lesions of systemic and discoid lupus erythematosus.

In 6 patients with untreated systemic lupus erythematosus (SLE) in the progressive stage, and in 6 with discoid lupus erythematosus (DLE), an analysis of inflammatory infiltrates was performed in situ using the avidin-biotin-peroxidase complex (ABC) method with monoclonal antibodies. In all patients, over 75% of the infiltrates reacted with the pan T-cell antibody OKT3, but only sporadically with that of B-cell OKB7. In addition, a large number of the infiltrates were OKIal-positive, indicating that they were in an activated state. Many OKT8-positive cells were seen infiltrating the epidermis especially in the vicinity of basal keratinocytes. Staining for T-cell subsets revealed that the proportion of OKT8-positive cells (suppressor/cytotoxic) was from 2 to 3 fold higher than that of OKT4-positive cells (helper/inducer) in lesions of SLE. On the contrary, in DLE, a predominance of OKT4-positive cells (the OKT4/OKT8 ratio was from 1:1 to 3:1) was observed. Thus, our results provide further evidence that these 2 main types of LE show quite contrary findings on immunohistochemical analysis of T-cell subsets, and that besides the humoral immune mechanism, the cell-mediated immune mechanism may be involved in the pathogenesis of these disorders.

Antibodies, Monoclonal↗
PubMed1985

Increased number of OKT6-positive dendritic cells in the hair follicles of patients with alopecia areata.

In 6 patients with untreated alopecia areata in the progressive stage, 6 in the stationary stage, and 6 normal individuals as controls, an in situ analysis of OKT6-positive dendritic cells in hair follicles, and peribulbar and intrabulbar infiltrates was performed using the avidin-biotin-peroxidase method with monoclonal antibodies. In controls, OKT6-positive dendritic cells were distributed only in the upper portions of hair follicles and were not observed in the bulbar area, and the percentage of these cells among all epithelial cells of the hair follicles was 1.0 +/- 0.1% (mean +/- SE). In stationary-stage patients, the distribution and the percentage of positive cells were the same as those for the controls (1.1 +/- 0.1%). In the progressive stage, however, positive cells were distributed in both the upper portions of the hair follicles and the bulbar area, and the percentage of positive cells (4.9 +/- 0.3%) was significantly higher than that of controls. Staining for T, B lymphocytes and T cell subsets in the peribulbar infiltrates revealed a predominance of OKT4-positive cells (the OKT4/OKT8 ratio was from 3:1 to 4:1). This indicates that the number of OKT6-positive dendritic cells increases in the hair follicles of progressive alopecia areata and that these cells may play an important role in cooperation with T cells in the pathogenesis of alopecia areata.

Alopecia Areata↗
PubMed1985

Effect of an N-succinyl-L-trialanine p-nitroanilide-hydrolyzing protease from pronase on glucose metabolism in mice.

Effect of an N-succinyl-L-trialanine p-nitroanilide-hydrolyzing protease (STA-protease) purified from Pronase on glucose metabolism was investigated by an intravenous injection into fasted mice. The maximum decrease in blood sugar level by STA-protease was observed 2 h after the injection of a dose of 5.0 mg/kg. The hypoglycemic activity was observed with other microbial proteases, such as Pronase E and subtilisin BPN', whereas no activity was found with the modified enzymes which almost wholly lost their proteolytic activities by the treatment with diisopropyl fluorophosphate or guanidine HCl. The increase in blood sugar level by epinephrine and the conversion of [1-14C] pyruvate into blood glucose were distinctly suppressed with STA-protease as well as 5-methoxyindole-2-carboxylic acid (MICA), an inhibitor of gluconeogenesis. A slight increase in glycogen content in diaphragm of mice was observed during 2-4 h after the injection of STA-protease, though it was markedly increased by insulin. In test of glucose tolerance, the increase in blood sugar was distinctly suppressed by insulin but not by STA-protease. Blood lactate level was not subjected to change by STA-protease, in Therefore, STA-protease may affect the in vivo metabolism of glucose in a different way from the in vitro action which has been reported to react with cell surface and consequently to mimic the actions of insulin.

Aluminum Silicates↗
PubMed1985

[Evaluation of the malignancy of breast carcinoma using flow cytometry].

Flow cytometric DNA analyses of single cell nuclei were performed on nuclear suspensions prepared from tumors of primary breast carcinomas in 40 patients operated on in our hospital. Fluorescence intensities of propidium-iodide stained cell nuclei were analyzed in a SHOWA DENKO SC-20 cell sorter. The DNA index and the percentage of S-phase cells and DNA aneuploidy were calculated from each DNA histogram. There was a tendency for more advanced nodal tumor involvement to have a higher DNA index, increasing DNA aneuploidy proportions and significantly larger percent of S-phase cells.

Adenocarcinoma, Mucinous↗
PubMed1985

[Pure sensory stroke due to midbrain hemorrhage--case report].

We had a case of patient with pure sensory stroke which was caused by the rapture of a cryptic angioma located on the mesencephalon. The patient was a 41-year-old woman. She visited our clinic with sudden onset of numbness in the left hand, arm, trunk, leg, and face, and of hyposmia of the left side on arising. CT scans revealed a small hematoma with calcification located on the right dorsal mesencephalon, which diminished in size correlating with her recovery of the sensation. Her symptoms were considered to be caused by this hematoma which destroyed only the trigeminothalamic and the spinothalamic tracts excluding the superior colliculi, oculomotor nucleus, and the medial leminiscus. By angiography we failed to demonstrate a cause of the hemorrhage, but the presence of a cryptic angioma was suggested, because the post angiographic CT scan exhibited enhancement of the lesion. Her olfactory sensation was also restored accompanied with the recovery of the superficial sensation, therefore the hyposmia seemed to be due to the damage of the trigeminothalamic tract.

Adult↗
PubMed1984

Effect of elastase on glucose and lipid metabolism in rat fat cells.

We examined the effects of elastase [EC 3.4.21.11] on lipogenesis, antilipolysis, and pyruvate dehydrogenase activity in rat epididymal adipose tissue in comparison with those of insulin and trypsin [EC 3.4.21.4]. The rate of conversion of [3-3H]-glucose into lipid in fat cells was stimulated by elastase, trypsin, and insulin. When fat pads were incubated with elastase, trypsin, or insulin in the presence of glucose, pyruvate dehydrogenase activity in the homogenate of the incubated fat pads was markedly increased. In the absence of glucose, elastase did not increase pyruvate dehydrogenase activity, though trypsin and insulin showed a slight but significant increase. Further, the increasing effect of elastase in the presence of glucose was inhibited by the addition of 3-O-methylglucose or phlorizin to the incubation mixture of the fat pads. Trypsin and insulin still showed a significant increase under similar conditions. When the homogenate of intact fat pads was incubated with elastase, the pyruvate dehydrogenase activity was progressively decreased with increase in the concentration of elastase. Concanavalin A showed an additive effect on the pyruvate dehydrogenase activity increase caused by elastase, whereas such an effect was not observed with insulin or H2O2. The stimulation of lipolysis by epinephrine in the fat cells was not suppressed by elastase, in contrast to trypsin and insulin. These results suggest that elastase reacts with the cell surface, facilitates glucose transport into the fat cells, and consequently affects glucose and lipid metabolism by somewhat different mechanisms from those of insulin and trypsin.

Adipose Tissue↗