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Biomedical subjects

H Ueda

Publications and source records attributed to H Ueda.

At least 559 records · Page 31Linked to original sources
PubMed1993

[Evaluation of the invasion of esophageal cancer to the aorta by cine-MR imaging].

We examined the usefulness of cine-MR imaging for evaluation of the invasion of esophageal cancer to the aorta in 12 cases. We used the technique of field echo pulse sequence. When the low intensity stripe was recognized between the tumor and the wall of aorta, we interpreted it as negative finding of the direct tumor invasion. By using this criteria, 11 of the 12 cases (92%) of the esophageal cancer for aortic wall invasion were correctly diagnosed as compared with 75% correct diagnosis by conventional MR imaging.

Aorta↗
PubMed1993

Immunocytochemical and immunogold localization of two prolactin isoforms in the same pituitary cells and in the same granules in the tilapia (Oreochromis mossambicus).

The tilapia pituitary secretes two forms of prolactin (tPRL) and a single growth hormone (tGH). The tPRLs share only 69% sequence identity and are designated tPRL177 and tPRL188 to indicate the number of amino acid residues in each isoform. Our aim was to develop specific antisera for detection of these three related polypeptides. Ten peptides corresponding to unique epitopes on the tPRLs and two peptides of tGH were synthesized using solid-phase methods, conjugated to carrier proteins, and used as immunogens for antibody production in rabbits. Select antisera for the tPRLs were highly specific, exhibiting only 1% cross-reactivity to the alternate tPRL under noncompetitive ELISA conditions at dilutions used in immunocytochemical analysis. The anti-tGH specifically bound to cells in the proximal pars distalis. Production of both tPRLs by a single cell type was indicated by the binding of both anti-tPRL177 and anti-tPRL188 to the same cells in the rostral pars distalis. Ultrastructural analysis of PRL-producing cells stained sequentially using the two different anti-tPRL antibodies labeled with immunogold of two size classes indicated that both tPRLs appear in the same granules. These findings suggest that the biological significance of two forms of PRL in the adult tilapia is not a function of differential regulation of two different classes of PRL cells or differential release of unique secretory granules.

Amino Acid Sequence↗
PubMed1993

Immunocytochemical study of membrane skeletons in abnormally shaped erythrocytes as revealed by a quick-freezing and deep-etching method.

Ultrastructures of membrane skeletons in spherocytic and elliptocytic erythrocytes were investigated immunocytochemically. Erythrocytes obtained from patients with hereditary spherocytosis (HS) and hereditary elliptocytosis (HE) were split open mechanically to obtain exposed cytoplasmic sides of erythrocyte membranes and were immunostained with anti-spectrin antibody. Replica membranes were prepared by a quick-freezing and deep-etching method and were checked by electron microscopy. The in situ membrane skeletons of normal erythrocytes consisted mainly of reticular patterns of spectrin filaments, which formed networks on the cytoplasmic sides of the cell membrane. In contrast, the membrane skeletons of abnormally shaped erythrocytes (HS and HE) were much less filamentous and more granular than those of normal erythrocytes. This abnormal organization in erythrocyte membrane skeletons may be one of the factors that induce abnormally shaped erythrocytes in HS and HE patients.

Adult↗
PubMed1993

EDTA-insensitive deacylation of phosphatidylinositol in porcine platelet membranes.

Pathways for EDTA-insensitive degradation of phosphatidylinositol (PI) were investigated in porcine platelet membranes and cytosol. The incubation of platelet membranes with [3H]glycerol-labeled PI in the presence of 2mM EDTA produced [3H]lysoPI and aqueous radioactive products, but not radioactive neutral lipids. The degradation in the membranes was optimal at pH8.0-9.0, while EDTA-insensitive hydrolysis was also observed in cytosol with optimal pH at pH7.0-9.0. The major water-soluble product was identified as glycerophosphoinositol. Under the conditions, [14C]arachidonate was released from 1-stearoyl-2-[14C]arachidonyl PI without accumulation of [14C]lysoPI. The deacylation activity preferred PI to phosphatidylcholine and phosphatidylethanolamine. Collectively, these results suggest that PI can be converted to lysoPI by phospholipase A2 in the absence of free Ca2+, providing the substrates for lysoPI-specific phospholipase C characterized earlier in porcine platelet membranes (Murase and Okuyama (1985) J.Biol.Chem. 260, 262-265).

Animals↗
PubMed1993

A microsatellite polymorphism in the human insulin receptor gene: a highly informative marker for linkage analysis.

To study genetic susceptibility to non-insulin dependent diabetes mellitus (NIDDM), association of insulin receptor gene, a candidate gene for NIDDM, with NIDDM was studied. A microsatellite polymorphism located in intron 2 of the human insulin receptor gene was detected by the polymerase chain reaction and used as a genetic marker. Eight different alleles were observed, indicating highly polymorphic nature of this marker. Although no association of this marker with NIDDM was observed in Japanese subjects, this marker will be useful for linkage studies of insulin receptor gene and genes located close to this gene on chromosome 19.

Alleles↗
PubMed1993

Immunocytochemical localization of copper-zinc superoxide dismutase in mouse olfactory mucosa.

Copper-zinc superoxide dismutase (Cu-Zn SOD) has been localized in mouse nasal mucosa. Immunocytochemical staining using polyclonal antibody against Cu-Zn SOD revealed endogenous Cu-Zn SOD in sustentacular cells in the olfactory area, and in ciliated epithelial cells in the respiratory area of the olfactory mucosa. Since these cells are located in the superficial portion of the olfactory mucosa, Cu-Zn SOD may serve as an intracellular antioxidant.

Animals↗
PubMed1993

Conservative treatment of burst fractures of the thoracolumbar and lumbar spine.

Twenty-three patients with burst fracture of the thoracolumbar and lumbar spine were treated nonoperatively. Among these 23 patients, 13 had a neurological deficit and 10 patients did not have such a deficit. Union of burst fractures occurred with conservative treatment in 22 out of 23 patients, but late operation was necessary in only one patient, who had an increasing kyphotic deformation. Of the 13 patients with neurological deficit, 8 showed full recovery and none had neurological deterioration. It was observed by CT scans that the narrowing of the spinal canals was progressively relieved by natural remodelling of the fragments retropulsed into the spinal canal.

Adolescent↗
PubMed1993

Possible involvement of beta-PKC rather than that of alpha-PKC in differentiation of 3T3-L1 cells to adipocytes.

The change of subspecies of protein kinase C (PKC) was studied in 3T3-L1 cells in terms of their differentiation to adipocytes. 3T3-L1 cells feasible to differentiate to adipocytes by exposure to 3-isobutyl-1-methylxanthine (IBMX) and dexamethasone had both alpha- and beta-PKC. However, 3T3-L1 cells unfurnished with such feasibility had only alpha-PKC. alpha-PKC, therefore, seems to be more deeply involved in differentiation of 3T3-L1 cells to adipocytes than alpha-PKC.

1-Methyl-3-isobutylxanthine↗
PubMed1993

A possible pathway of phosphoinositide metabolism through EDTA-insensitive phospholipase A1 followed by lysophosphoinositide-specific phospholipase C in rat brain.

Incubation of [2-3H]glycerol-labeled phosphatidylinositol with a crude cytosol fraction of rat brain in the presence of EDTA yielded [3H]lysophosphatidylinositol predominantly without accumulation of labeled monoacylglycerol and diacylglycerol. The pH optimum of this phospholipase A activity was 8.0. The activity for phosphatidylinositol was twofold higher than for phosphatidylethanolamine, whereas phosphatidylcholine, phosphatidylserine, and phosphatidic acid were not hydrolyzed significantly under the conditions used. The phospholipase A activity for phosphatidylethanolamine was resolved in part from that for phosphatidylinositol by ammonium sulfate fractionation of the cytosol, indicating the existence of at least two forms of EDTA-insensitive phospholipase A. The positional specificity of the phosphatidylinositol-hydrolyzing activity was found to be that of a phospholipase A1, as radioactive lysophosphatidylinositol was produced from 1-stearoyl-2-[1-14C]arachidonyl-sn-glycero-3-phosphoinositol without release of free arachidonate. A phospholipase C activity specific for lysophosphoinositides was found in a membrane fraction from rat brain, which was similar to that characterized in porcine platelets. The phospholipase C was demonstrated to hydrolyze the 2-acyl isomer as well as the 1-acyl isomer of lysophosphatidylinositol. Taken together, our results suggest a possible pathway through which phosphatidylinositol is selectively degraded to the 2-acyl isomer of lysophosphatidylinositol in a Ca(2+)-independent manner, and subsequently converted to a 2-monoacylglycerol in rat brain.

Animals↗
PubMed1993

Is an additional vaccination necessary for a successful second pregnancy in unexplained recurrent aborters who were successfully immunized with their husband's lymphocytes before the first pregnancy?

PROBLEM: Is an additional immunotherapy necessary or not for patients who have obtained successful results after initial immunotherapy? METHODS: The successive pregnancy outcome was analyzed in 22 patients out of 29 unexplained recurrent aborters who had undergone immunotherapy with their husband's lymphocytes according to our previously reported protocol (Takakuwa et al., Am J Reprod Immunol Microbiol. 10:1-9, 1986; Takakuwa et al., Am J Reprod Immunol. 23:37-41, 1990) and had obtained successful outcome between January 1983 and December 1989. In addition, the alteration of blocking antibodies (BAbs), which was evaluated by a one-way mixed lymphocyte culture reaction (MLR) blocking assay between the spouses, was analyzed in 26 patients out of 29. RESULTS: None of the 22 patients underwent further immunotherapy because a significantly high titer of MLR-BAbs had been detected before the new pregnancy. In 19 out of the 21 patients (90.5%), pregnancy was successful. CONCLUSION: Additional immunotherapy is not necessary for patients who have obtained successful results after the initial immunotherapy and are positive for MLR-BAbs after their first delivery.

Abortion, Habitual↗
PubMed1993

Arrhythmogenic right ventricular dysplasia: evaluation with electron-beam CT.

The authors evaluated four cases of arrhythmogenic right ventricular dysplasia (ARVD) with electron-beam computed tomography (CT). Except for magnetic resonance imaging, non-invasive imaging techniques such as two-dimensional echocardiography and radionuclide angiography cannot always depict the abnormal morphology of the right ventricle. Definite diagnosis of ARVD requires the use of invasive cardiac cineangiography. Proper identification of right ventricular involvement in ARVD with CT helps in the diagnosis. Volume-mode CT clearly depicted an enlarged right ventricle with a scalloped surface of the free wall, conspicuous trabeculations with low attenuation, and abundant epicardial adipose tissue, which are characteristics of ARVD. Cinemode CT revealed reduced right ventricle function and abnormal wall motion. The authors propose that millisecond-order, electron-beam CT is a suitable noninvasive examination for evaluating the morphologic characteristics of the right ventricle and its function in ARVD.

Adult↗
PubMed1993

Syntheses and inhibitory effects on gastric lesions of 4-guanidinomethylbenzoic acid arylamides.

A novel series of 4-guanidinomethylbenzoic acid (GMBA) arylamides was synthesized. Several showed more potent inhibitory effects on stress-induced gastric lesion in rats than cetraxate. We selected 4-guanidinomethylbenzoic acid (2'-ethoxycarbonyl)phenylamide 3 for further pharmacological assessments because it had low toxicity. Compound 3 showed significant inhibitory effects on stress-, HCl-ethanol- and indomethacin-induced gastric lesions and gastric secretion, the ED50 values being 34.4, 45.0 and 23.0 mg/kg (p.o.) and 240 mg/kg (i.d.), respectively. Furthermore, this compound restored the reduction of gastric mucus caused by the stress-loading and inhibited compound 48/80-induced ulcer.

Animals↗
PubMed1993

Syntheses and inhibitory effects on gastric lesions of trans-guanidinomethylcyclohexane carboxylic acid arylamides.

A novel series of trans-guanidinomethylcyclohexanecarboxylic acid (trans-GMCHA) arylamides was synthesized. The several trans-GMCHA arylamide derivatives showed more potent inhibitory effects on the stress- and HCl-ethanol-induced gastric ulcers than cetraxate in rats. In acute toxicity studies in mice, most amides showed such severe toxicity that all mice injected with these compounds (50 mg/kg, i.p.) died. However, mice injected with the trans-GMCHA (2'-,3'- and 4'-ethoxycarboxy)phenylamide (7, 8 and 9) which bear an alkyloxycarbonyl group at benzene ring survived. From these results, trans-GMCHA (2'-ethoxycarbonyl)phenylamide (7) was selected as a promising anti-ulcer agent.

Animals↗
PubMed1993

Serum collagenase-like peptidase activity correlated with bone mineral density. A study of 102 elderly women.

To clarify the relationship between collagenase-like peptidase (CL-peptidase) and bone metabolism, serum CL-peptidase activity and bone mineral density (BMD) in the lumbar spine, measured by dual energy X-ray absorptiometry (DEXA), were determined in 102 women with a mean age of 70 (41-94) years. The serum activity of CL-peptidase increased slightly with age up to the 8th decade, and then decreased. Next, we classified the subjects into the following 2 groups by age: a postmenopausal group 50-69 years, and an elderly group over 70 years. In the younger group serum CL-peptidase activity did not correlate with BMD, while in the elderly it did. Our result suggests that serum CL-peptidase activity may reflect bone resorption in elderly women.

Adult↗
PubMed1993

[The effect on age for the relationship between hypertension and glucose intolerance].

Recently, high frequencies of glucose intolerance and insulin resistance have been reported in patients with hypertension. However, both blood pressure and glucose tolerance are influenced by age. To investigate the effect of age on the interaction between blood pressure and glucose tolerance, we analyzed blood pressure and glucose tolerance in otherwise healthy subjects (n = 576) who underwent an oral glucose tolerance test and blood pressure measurement as a part of a healthy care program. The prevalence of DM and IGT were significantly higher in the hypertensive group than in the normotensive group. When the subjects were divided into three groups according to their age (less than 50 years old, between 50 and 60 years old and more than 60 years old), the prevalence of glucose intolerance (DM or IGT) was significantly higher in the hypertensive group than in the normotensive group in subjects under 50 years old, but not in subjects between 50 and 60 years old or over 60 years old. In the subjects classified as having normal glucose tolerance, the incremental area of glucose under the curve of 75g-OGTT was significantly higher in the hypertensive group than in the normotensive group. These data suggest that hypertension is associated with glucose intolerance in Japanese population and that age significantly affects this interaction.

Aged↗
PubMed1993

[Evoked otoacoustic emissions: a comparison between responses from humans and guinea pigs].

Transiently evoked otoacoustic emissions (TEOAEs) from 16 normal human ears and 26 normal guinea pig ears were measured with the ILO 88 (ver 3.91). The following results were obtained: 1) In one animal ear, waveforms of TEOAEs were unchanged after administration of muscle relaxants, and showed reversible changes before and after asphyxia. 2) In 3 guinea pig and 2 human ears, the input-output functions showed a strong saturation at higher stimulus levels, when measurement of the highest peak-to-peak amplitude was used as the output energy. 3) Highest peak frequencies (HPFs) of TEOAEs were distributed at around 1 kHz in human ears but were concentrated around frequencies of 2 kHz to 3 kHz in almost all guinea pig ears. 4) Latencies of predominant TEOAEs in human and guinea pig ears were 9.26 +/- 1.89 msec and 2.37 +/- 0.47 msec, respectively. Endpoint times of TEOAEs were 16.37 +/- 1.79 msec and 5.74 +/- 1.10 msec, respectively. Both indicators in human ears were 3-4 times longer than those in guinea pig ears. From these results, we conclude that click-evoked TEOAEs can be detected even in guinea pigs. In addition, our measurements showed that HPFs of TEOAEs in guinea pig ears were higher than those in human ears, and that latencies and durations in guinea pig ears were much shorter than those in human ears. The higher HPFs of TEOAEs in guinea pig ears may be attributable to higher resonant frequencies in the middle ear than are found in human ears.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1993

WS1279, a novel lipopeptide isolated from Streptomyces willmorei. Biological activities.

WS1279, a new lipopeptide isolated from the fermentation broth of Streptomyces willmorei No. 1279, stimulated the proliferation of mouse bone marrow cells in vitro and accelerated the recovery of granulocyte counts in bone marrow from leukopenia induced by mitomycin C (MMC) in mice. The glycerylcysteine moiety of WS1279 is necessary and the lipid peptide structure is required for manifestation of full stimulating activity in vitro. WS1279 was the most effective on the proliferation of bone marrow cells among the tested immunostimulants in vitro. However, the effect of WS1279 on restoration of reduced granulocyte counts in MMC-induced leukopenia in mice was less than that of FK-565, lipopolysaccharide, picibanil or forphenicinol. WS1279 augmented host resistance to infection with Staphylococcus aureus 47 in normal and immunosuppressed mice.

Adjuvants, Immunologic↗