[Immunogenicity and protective activity of glycoprotein extracted from rabies virus: results from comparison of preparations obtained by different methods of purification (author's transl)].
A rabies vaccine was prepared from virus grown on primary cultured bovine foetal kidney cells. After beta-propiolactone inactivation of the virus, the vaccine was partially purified by zinc acetate precipitation, G-75 Sephadex chromatography, and ultracentrifugation prior to a non ionic detergent solubilisation. A glycoprotein fraction with protective capacity was extracted by Triton X100 from the spikes of the inactivated rabies virus. An electrophoretically homogenous glycoprotein was found to have higher values of protective activity compared to the initial tissue culture vaccine. T-e yield from this extraction method was from 10 to 20 per cent, based upon an immunological assay.
Adjuvants, Immunologic↗