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Biomedical subjects

H Thompson

Publications and source records attributed to H Thompson.

At least 145 records · Page 8Linked to original sources
PubMed1988

Studies on canine parvovirus infection: preparation of challenge virus.

Two techniques, adsorption on to hydroxylapatite and density gradient centrifugation, were investigated as prospective methods for the large scale purification of canine parvovirus from faecal suspensions. Adsorption with hydroxylapatite successfully removed virus from faecal material. However, the resultant virus was contaminated and some virus was left behind in the faecal suspension. Repeated adsorption with hydroxylapatite appeared to result in some damage to the virus particles. In contrast, density gradient centrifugation provided a simple, economical method of purification which yielded uncontaminated, infectious virus. The final method, using both isopyknic and rate zonal centrifugation is described.

Animals↗
PubMed1988

Multiple bidirectional alterations of phenotype and changes in proliferative potential during the in vitro and in vivo passage of clonal mast cell populations derived from mouse peritoneal mast cells.

Mouse peritoneal mast cells (PMC) express a connective tissue-type mast cell (CTMC) phenotype, including reactivity with the heparin-binding fluorescent dye berberine sulfate and incorporation of [35S] sulfate predominantly into heparin proteoglycans. When PMC purified to greater than 99% purity were cultured in methylcellulose with IL-3 and IL-4, approximately 25% of the PMC formed colonies, all of which contained both berberine sulfate-positive and berberine sulfate-negative mast cells. When these mast cells were transferred to suspension culture, they generated populations that were 100% berberine sulfate-negative, a characteristic similar to that of mucosal mast cells (MMC), and that synthesized predominantly chondroitin sulfate [35S] proteoglycans. When "MMC-like" cultured mast cells derived from WBB6F1-+/+ PMC were injected into the peritoneal cavities of mast cell-deficient WBB6F1-W/Wv mice, the adoptively transferred mast cell population became 100% berberine sulfate-positive. In methylcellulose culture, these "second generation PMC" formed clonal colonies containing both berberine sulfate-positive and berberine sulfate-negative cells, but exhibited significantly less proliferative ability than did normal +/+ PMC. Thus, clonal mast cell populations initially derived from single PMC exhibited multiple and bidirectional alterations between CTMC-like and MMC-like phenotypes. However, this process was associated with a progressive diminution of the mast cells' proliferative ability.

Animals↗
PubMed1987

Plateletapheresis using single vein access: a comparison of Haemonetics V-50 and Fenwal CS-3000 blood cell separators.

Donors were randomly selected for plateletapheresis using either the Haemonetics V-50 surge procedure or the Fenwal CS-3000 with special modifications. Six procedures using the V-50 were performed with 6 collection cycles. Platelet yields averaged 3.9 X 10(11) with an average white cell contamination of 3.8 X 10(8). Ten procedures were performed with 8 collection cycles. Platelet yields averaged 4.5 X 10(11) with an average white cell contamination of 1.2 X 10(8). CS-3000 procedures were performed using either 4,000 or 4,800 ml whole blood processed as the endpoint. Concentrates obtained using 4,000 ml (n = 10) as the endpoint contained 3.9 X 10(11) platelets with a white cell contamination of 9.2 X 10(8). A 4,800 ml (n = 5) endpoint produced concentrates with a yield of 4.5 X 10(11) and white cell contamination of 10.4 X 10(8).

Blood Component Removal↗
PubMed1986

Crohn's disease involving the penis.

Perianal complications of Crohn's disease occur in 25-70% of patients but perineal and genital lesions are rare. Treatment is controversial and there is a risk of recurrent or persistent disease. We report two cases of Crohn's disease involving the penis, one with multiple scrotal urinary fistulae, partial destruction of the proximal urethra, and ulceration of the penile shaft; the other with metastatic cutaneous ulceration of the penile shaft. The second case is particularly unusual in that the patient presented at the VD clinic as a case of syphilis.

Adolescent↗
PubMed1986

Mammographic identification and biopsy of occult breast cancer.

During a 9 year period we excised 70 suspicious non-palpable breast lesions detected by mammography. We found the most effective method of preoperative localisation to be insertion of a self retaining wire, and this procedure was facilitated by a Perspex grid incorporated in the mammography equipment. Thirty biopsies (43%) were found to be malignant, of which 14 contained invasive and 16 in situ carcinoma. The presence of malignancy could not be predicted from the mammographic appearance, although in situ cancer was always associated with microcalcification.

Adult↗
PubMed1985

A distinct class of vertebrate collagen genes encodes chicken type IX collagen polypeptides.

Type IX collagen is a disulfide-bonded protein first isolated from hyaline cartilage. The structure of this collagen is unusual in that the molecules contain three triple-helical domains interspersed with noncollagenous regions. The molecules are heterotrimers composed of three genetically distinct polypeptide chains. In our laboratory, cDNAs specific for two of these polypeptide chains have recently been isolated. Here we report on the isolation of genomic clones by use of these cDNAs as probes for screening a chicken genomic library. Nucleotide sequence analysis of these clones shows that the exon structure of type IX collagen genes is fundamentally different from the exon structure of the genes for the fibrillar collagen types I-III. Whereas the sizes of exons in fibrillar collagen genes are related to a basic 54-base-pair coding unit, the exons of type IX collagen genes show a large variation in size and do not appear to be related to a 54-base-pair unit. We propose, therefore, that type IX collagen genes belong to a class of vertebrate collagen genes distinct from that of fibrillar collagens.

Amino Acid Sequence↗
PubMed1985

Epstein-Barr virus infections in homosexual men with chronic, persistent generalized lymphadenopathy.

The status of infection by the Epstein-Barr virus (EBV) in 20 patients with chronic, persistent generalized lymphadenopathy was evaluated with use of three parameters: antibodies to EBV, EBV excretion, and EB virocytemia. Results were compared with those obtained from two groups of control subjects: patients with infectious mononucleosis and healthy EBV-seropositive adults. Profiles of antibody to EBV and the prevalence of EBV excretion were essentially similar for study subjects and healthy control subjects, but the level of EB virocytemia was significantly higher in study subjects than in healthy control subjects.

Adult↗