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H Terashima

Publications and source records attributed to H Terashima.

At least 37 records · Page 2Linked to original sources
PubMed1999

Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins.

The final destination of glycosylphosphatidylinositol (GPI)-attached proteins in Saccharomyces cerevisiae is the plasma membrane or the cell wall. Two kinds of signals have been proposed for their cellular localization: (i) the specific amino acid residues V, I, or L at the site 4 or 5 amino acids upstream of the GPI attachment site (the omega site) and Y or N at the site 2 amino acids upstream of the omega site for cell wall localization and (ii) dibasic residues in the region upstream of the omega site (the omega-minus region) for plasma membrane localization. The relationships between these amino acid residues and efficiencies of cell wall incorporation were examined by constructing fusion reporter proteins from open reading frames encoding putative GPI-attached proteins. The levels of incorporation were high in the constructs containing the specific amino acid residues and quite low in those containing two basic amino acid residues in the omega-minus region. With constructs that contained neither specific residues nor two basic residues, levels of incorporation were moderate. These correlations clearly suggest that GPI-attached proteins have two different signals which act positively or negatively in cell wall incorporation for their cellular localization.

Amino Acid Sequence↗
PubMed1999

Colon-specific delivery of budesonide with azopolymer-coated pellets: therapeutic effects of budesonide with a novel dosage form against 2,4,6-trinitrobenzenesulphonic acid-induced colitis in rats.

The objective of this study was to achieve colon-specific delivery of budesonide using azopolymer-coated pellets and to accelerate healing of 2,4,6-trinitrobenzenesulphonic acid sodium salt (TNBS)-induced colitis in rats. After oral administration of azopolymer-coated pellets containing budesonide, a significant increase was observed in the therapeutic effects of the drug accompanied by a decrease in its systemic adverse effects when compared with oral administration in saline or rectal administration by enema. In addition, with the use of the colon-specific oral dosage form the dose of budesonide could be reduced. These results suggested that azopolymer-coated pellets may be a useful dosage form for the colon-specific delivery of budesonide as an anti-inflammatory steroid drug to bring about the healing of TNBS-induced colitis in rats.

Administration, Oral↗
PubMed1999

cDNA sequence of bovine thioredoxin.

In this paper, we report the cDNA sequence of bovine thioredoxin. We determined the full-length cDNA sequence of bovine thioredoxin by RT-PCR, 5'-RACE and 3'-RACE methods. Currently, the thioredoxin cDNA sequences of only five mammalian species (human, macaca, mouse, ovine and rat) are registered in the GenBank database. We performed sequence comparisons on the total cDNA sequence and the coding region, and produced a multialignment between the amino acid sequences of bovine and other mammalian thioredoxins. The amino acid sequences of thioredoxins are highly conserved among mammalian species, for example, only one difference exists between the amino acid sequences of bovine and ovine thioredoxin.

Animals↗
PubMed1999

cDNA sequence of bovine thioredoxin.

In this paper, we report the cDNA sequence of bovine thioredoxin. We determined the full-length cDNA sequence of bovine thioredoxin by RT-PCR, 5'-RACE and 3'-RACE methods. Currently, the thioredoxin cDNA sequences of only five mammalian species (human, macaca, mouse, ovine and rat) are registered in the GenBank database. We performed sequence comparisons on the total cDNA sequence and the coding region, and produced a multialignment between the amino acid sequences of bovine and other mammalian thioredoxins. The amino acid sequences of thioredoxins are highly conserved among mammalian species, for example, only one difference exists between the amino acid sequences of bovine and ovine thioredoxin.

Amino Acid Sequence↗
PubMed1999

[Usefulness and problems of total hepatic vascular exclusion in liver surgery].

Total hepatic vascular exclusion (THVE) is an useful method enabling safe and sure hepatic resection in patients with liver tumors adjacent to the large hepatic veins or inferior vena cava (IVC), tumor thrombi, invasion of the IVC, etc. To avoid serious hypotension during THVE, test clamping of the IVC prior to the procedure is indispensable. Hemodynamics should be carefully maintained by blood transfusion and sufficient infusion of colloidal and electrolyte solutions during THVE. The veno-venous bypass method which shunts blood from the IVC and portal vein to the superior vena cava enables prolongation of the period of THVE and is useful to avoid postoperative renal dysfunction. In situ liver perfusion with cold solution during THVE is an additional modality by which the liver is protected from warm ischemic injury and the duration of THVE can be further prolonged. However, the maximum duration of THVE is still controversial, especially in patients with chronic liver damage. The most appropriate method for THVE should be carefully chosen in each case by considering the type of lesion, liver function, and the goal of the surgery.

Constriction↗
PubMed1998

Amino acid sequence requirement for efficient incorporation of glycosylphosphatidylinositol-associated proteins into the cell wall of Saccharomyces cerevisiae.

During cell wall biogenesis in Saccharomyces cerevisiae, some glycosylphosphatidylinositol (GPI)-attached proteins are detached from GPI moieties and bound to beta-1,6-glucan of the cell wall. The amino acid sequence requirement for the incorporation of GPI-attached proteins into the cell wall was studied by using reporter fusion proteins. Only the short omega-minus region composed of five amino acids, which is located upstream of the omega site for GPI attachment, determined the cellular localization of the GPI-associated proteins. Within the omega-minus region, amino acid residues at the omega-4 or -5 and omega-2 sites were important for the cell wall incorporation. Yap3p, a well characterized GPI-anchored plasma membrane aspartic protease, was localized in the cell wall when the omega-minus region was mutated to sequences containing Val or Ile at the omega-4 or -5 site and Val or Tyr at the omega-2 site.

Amino Acid Sequence↗
PubMed1998

[Continuous injection therapy of morphine solution for terminally-ill patients at home].

We surveyed 21 terminally-ill patients with cancer pain who received continuous injection therapy of morphine at home in the three years from 1995 to 1997. The period of home therapy was 179.3 +/- 257.5 (mean +/- SD) days. The period of morphine injection was 39.2 +/- 29.1 days. Morphine solution was injected intravenously via a catheter inserted in a central vein in all patients. The instrument used most frequently for injection was the seven-day type disposable balloon pump (n = 17, 81.0%). The maximum dosage of morphine in the period of home therapy was 57.3 +/- 30.6 mg per day. Nine patients (42.9%) seldom used supplemental analgesics, while 11 patients (52.4%) used them sometimes. Nine patients had some trouble with continuous injection therapy of morphine. The following were problems: accidents with the injection instruments (insufficient injection volume, delay of drug supply, etc.) and of the intravenous catheters (accidental removal of catheter, occlusion of catheter, etc.). Eleven patients (52.4%) died at home, and 10 patients (47.6%) were hospitalized again. Continuous morphine injection therapy is an effective strategy for cancer pain management at home.

Analgesics, Opioid↗
PubMed1998

[Current status of cancer pain control in home hospice care--assessment by family members].

To improve the quality of life of terminally ill cancer patients, we care for them in the home whenever possible, making use of home parenteral nutrition (HPN) and continuous intravenous infusion of morphine. The purpose of this study was to assess the current status of and problems associated with our home hospice care program, with emphasis placed on the effectiveness of cancer pain control as viewed by the family of the patient. A questionnaire was sent to six families of patients who died at home. The members of each family were divided into two groups (spouse group or non-spouse group) and evaluated for their reaction to the patient's suffering. It was found that the two groups displayed a different reaction to the patient's suffering. The difference may have derived from the different mixture of objectivity and affection in each group. Therefore, we believe it is necessary to provide emotional support to each group based on this point of view. All respondents expressed a desire to receive home hospice care if they themselves were afflicted with end-stage cancer. This result showed that overall our home hospice care was successful. We intend to proceed with our program to provide patients and their families with more comfortable care, paying due regard to the difference in the reaction of family members to the patient's suffering depending on their relation to patient.

Aged↗
PubMed1997

Human endothelin receptors ET(A) and ET(B) expressed in baculovirus-infected insect cells--direct application for signal transduction analysis.

We expressed human endothelin receptors, ET(A) and ET(B), in insect Sf9 cells infected by recombinant baculoviruses that contained the respective cDNAs. Ligand-binding experiments showed that the two expressed receptors have the same affinities as observed for the receptors in mammalian cells, i.e. the ET(A) receptor showed an affinity order of ET-1 > or = ET-2 >> ET-3, and the ET(B) receptor remained nonselective for three isopeptide ligands. The ET(B) receptor was purified by affinity chromatography with K9-biotinyl-ET-1 without losing the ligand-binding activity from the membrane of infected Sf9 cells. Protein chemical analysis of the purified ET(B) receptor showed that it is glycosylated, and that the N-terminal 38-amino-acid peptide is susceptible to proteolytic digestion, resulting in a small 35-kDa receptor like that found in the human placenta. Surprisingly, the infected and unlysed cells showed a strong intracellular Ca2+ concentration increase ([Ca2+]i), which was generated by a unique signal-transduction pathway consisting of the insect GTP-binding protein and human endothelin receptors expressed in the late phase of virus infection. Due mainly to an efficient expression (over 200,000 receptors/cell), to a low background owing to no endogenous homolog receptor in insect Sf9 cells, and to a sensitive fluorescent reagent Fura-2, this insect Sf9 cell system can detect the [Ca2+]i induced by picomolar levels of endothelin-receptor. We propose that this highly sensitive system be used to screen for potential antagonists/agonists of endothelin receptors.

Amino Acid Sequence↗
PubMed1997

[The necessity of mediastinal lymph node dissection for yolk sac tumor of the anterior mediastinum].

Combined treatment with preoperative chemotherapy and surgical resection is the universally accepted effective treatment of mediastinal yolk sac tumors. However, a problem on the need and efficacy of lymph node dissection in mediastinal yolk sac tumors has been not enough discussed in the past. We reported a case of mediastinal yolk sac tumor with nodal metastasis. As described in this paper, the resection of the primary lesion including the regional lymph nodes en bloc should be recommended for mediastinal yolk sac tumors.

Adolescent↗
PubMed1996

A point mutation within each of two ATP-binding motifs inactivates the functions of elongation factor 3.

We have investigated how point mutations in the two ATP-binding motifs (G(463)PNGCGK(469)ST and G(701)PNGAGK(707)ST) of elongation factor 3 (EF-3) affect ribosome-activated ATPase activity of EF-3, polyphenylalanine synthesis, and growth of Saccharomyces cerevisiae. The point mutation impaired the ribosome-activated ATPase activity of EF-3, when glycine(463 and 701) and lysine(469 and 707) were replaced with valine and arginine, respectively. Thus, each glycine and lysine residue in both ATP-binding motifs is indispensable for EF-3's binding with ATP and the ensuing generation of ribosome-activated ATPase activity. Additionally, the mutant EF-3s did not catalyze polyphenylalanine synthesis in vitro when each glycine(463 and 701) was replaced with valine. The mutant EF-3s did not support cell growth in TEF3-disrupted S. cerevisiae, when each lysine(469 and 707) and glycine(463) was replaced with arginine and valine, respectively. Thus, each of the two ATP-binding motifs of EF-3 is indispensable for the ribosome-activated ATPase activity of EF-3, which is required for protein synthesis and cell growth in S. cerevisiae.

Adenosine Triphosphatases↗
PubMed1996

Radiotherapy after hyperbaric oxygenation for malignant gliomas: a pilot study.

The results of radiotherapy combined with hyperbaric oxygen in 9 patients with malignant glioma were compared with those of radiotherapy without hyperbaric O2 in 12 patients. This is the first report of a pilot study of irradiation immediately after exposure to hyperbaric O2 in humans. All patients receiving this treatment showed more than 50% regression of the tumor, and in 4 of them, the tumors disappeared completely. Only 4 out of 12 patients without hyperbaric O2 showed decreases in tumor size, and all 12 patients died within 36 months. So far, this new regimen seems to be a useful form of radiotherapy for malignant gliomas.

Adolescent↗
PubMed1996

[Primary carcinosarcoma of TE lung--a report of two cases].

Carcinosarcoma is a rare lung tumor and accounts for less than 0.3% of primary lung malignancies. Since the first description by Kika in 1908, only 36 cases with this kind of tumor have been appeared in the Japanese literatures by 1993. This report presents our surgical experiences of two cases with carcinosarcoma of the lung confirmed by pathological examination. Case 1: 64-year-old male underwent left lower lobectomy with lymph node resection. The patient has been well 27 months after the operation without tumor recurrence. Case 2: was a 75-year-old male, who underwent left upper lobectomy, partial resection of left lower lobe (S6) with lymph node resection. This patient died of aspiration pneumonia 90 days after successful resection of the tumor. In both patients, resected lung tumors were diagnosed to be true carcinosarcoma by histopathological examinations. True carcinosarcoma is defined to contain both cancelous and sarcomatous elements. Sarcomatous elements may differentiate into rhabdomyosarcoma, osteosarcoma and so on, or they may have non-epithelial elements demonstrated by electron microscopy or immunohistochemical studies. We reviewed the 36 cases with carcinosarcoma of the lung reported in Japanese literatures with special consideration of their histopathological findings. The prognosis of the patients with this rare tumor is also discussed according to the TMN stages.

Aged↗
PubMed1996

Membrane-bound carbonic anhydrase activity in the rat corneal endothelium and retina.

Localization of carbonic anhydrase (CA) was investigated in the rat eye by light and electron microscopy, and the inhibitory effects on CA distribution in the rat eye were compared between dorzolamide and acetazolamide. The cobalt sulfide histochemical method was used to study the CA localization. Acidic osmification was carried out for postfixation of the electron microscopic observations. The inhibition of CA activity was examined with different concentrations of acetazolamide and dorzolamide. The cytoplasm, lateral cell membranes and karyoplasm of the corneal endothelium showed CA activity. In the retina, the processes and cell bodies of Müller cells, the tips of the outer segments of the rods, and apical villi and basolateral membranes of the pigment epithelium indicated CA activity. CA activity was inhibited by dorzolamide at lower concentrations than needed for acetazolamide and only the CA activity of the nerve fiber layer remained at 10(-6) M of acetazolamide and 5 x 10(-7) M of dorzolamide. The presence of membrane-bound CA activity was confirmed in the corneal endothelium and Müller cells. CA activity was also demonstrated in the apices of rod outer segments and scleral fibroblasts. The CA inhibition test revealed that dorzolamide was a stronger CA inhibitor than acetazolamide and that the CA activity of the nerve fiber layer was strongest in the rat eye.

Acetazolamide↗
PubMed1995

Ligand binding domain of the human endothelin-B subtype receptor.

We have employed both protein chemical and molecular biological approaches to determine the ligand binding domain of the endothelin-B subtype (ETB) receptor. The human ETB receptor purified from human placenta by using affinity chromatography was cross-linked with 125I-labeled endothelin-1 (ET-1) and then incubated in the presence of trypsin or thermolysin under nondenaturing conditions. The N-terminal amino acid sequence of the radiolabeled polypeptide encompassed approximately 115 amino acid residues starting from Ile85 of the human ETB receptor. This was confirmed by experiments in which the binding activity of endothelin-1 to various chimeric endothelin receptors was monitored in the presence and absence of competitive endothelin receptor antagonists such as BQ-123 and bosentan. The region from Ile138 to Ile197 (60 amino acid residues) of the ETB receptor was found to interact with both antagonists. Therefore, this sequence was determined to be the ligand binding domain. In addition, we found that part of the N-terminal domain in close proximity to the first transmembrane region was required for the ligand binding activity of the ETB receptor, and the 12 amino acid residues from Ser390 to Leu401 at the proximal cytoplasmic tail are perhaps necessary to maintain the ligand binding site in active form. The cysteine rich region from residue 400 to residue 403 in the C-terminus of the ETB receptor is involved in coupling of the guanine nucleotide-binding regulatory protein for ET-1-induced signal transduction.

Amino Acid Sequence↗
PubMed1995

Purification and characterization of aminopeptidase M from muscle and mucosa of the pig intestine.

The aim of this investigation was to purify aminopeptidase M (APM) from the muscle layer of the small intestine, to compare it with APM of the mucosa and kidney, and to examine the degradation of gastrointestinal neural and hormonal peptides by muscle APM. APM was purified from the muscle and mucosa of the pig small intestine by DEAE-Sepharose and immuno-affinity chromatography. The specific activity of APM from muscle, mucosa, and kidney was 3900, 3000, and 3800 nmol/min per mg protein, respectively (substrate [Leu5]enkephalin). Muscle and mucosa APM contained four protein bands with apparent molecular weights of 150, 110, 73, and 52 kDa. Kidney APM contained three protein bands of 150, 110, and 56 kDa. The 150, 110, and 52/56 kDa bands cross-rected with an APM antiserum. APM from each tissue degraded [Leu5]enkephalin and [Met5]enkephalin, but not cholecystokinin-8, gastrin releasing peptide-10, somatostatin-14, substance P, and vasoactive intestinal peptide. The enzymes were identically inhibited by APM antiserum, amastatin, bestatin, actinonin, and 1, 10 phenanthroline. Non-mucosal APM may degrade enkephalins and terminate their biological actions.

Aminopeptidases↗
PubMed1995

[Radiotherapy for carcinoma of the esophagus in aged patients].

One hundred fifty-four patients with esophageal carcinoma were treated with either irradiation alone or irradiation combined with surgery at the University of Occupational and Environmental Health Hospital between January 1980 and February 1992. The number of patients 75 years old and older was 25. In patients 74 years old and younger, the overall five-year survival rate by Kaplan-Meier method was 24.5%. The survival rate was best in the patients who were treated by a combination of irradiation and surgery. In patients 75 years old and older, the one-year survival rate was 59%, and the three-year rate was 20%. Aged patients had a tendency to be worse in performance status, and there was no correlation between treatment modality and survival time. We conclude that radiotherapy is useful for treating esophageal cancer in aged patients particularly when maintenance of the quality of life is considered.

Adult↗