The oxygen consumption (MO2) response over a 4 h period of exposure to altered ambient O2 (air, 10, 15, 40, 60, 80 and 100%), helium (He) and sulfur hexafluoride (SF6) environments was determined for young (12 days old) and late (16 and 18 days old) embryos, externally pipped (EP) eggs and just hatched chicks (hatchlings) of the domestic fowl. The young embryos were insensitive to hyperoxic gas mixtures and to He exposure, while the late embryos increase their MO2 in hyperoxic environments, independently of O2 concentration, and also in a He atmosphere. Both the young and late embryos responded to SF6 exposure with decreasing MO2, as SF6 reduces O2 diffusivity through the eggshell. The MO2 of EP eggs and hatchlings in He and SF6 varied very widely, the effects of altered diffusivity being insignificant. In hypoxic environments in which the MO2 decreased, the fall of MO2 became smaller as embryos developed and particularly after they pipped the shell and hatched. In an atmosphere of 10% O2, the MO2 of all embryos in the egg before hatching decreased to below 10% of the control after 4 h, while in hatchlings the MO2 remained above 80% of the control. As all embryos in situ in the egg depend entirely or partly on diffusion in order to obtain O2, this emphasizes the limitation of the diffusive process. A 4 h exposure to 10% O2 was lethal for embryos in the egg even if they had pipped the shell and were breathing air with the lungs.

Animals↗

PubMedDate not supplied

Comparisons between invasive and noninvasive determinations of embryonic heart rate in chickens.

The embryonic heart rate (HR) of chicken eggs was determined by ballistocardiography, electrocardiography, impedance-cardiography and acoustocardiography. The effects of these different methods on the HR were examined for young (12-day-old) and late (16- and 18-day-old) embryos. Ballistocardiography is a non-invasive method. Nevertheless, the mean HRs determined using this method at 5-hr intervals were significantly different in many embryos. This difference was caused by the natural variability of embryonic HR. The statistical analyses indicated that any of these four methods did not influence the HR measurement and can be used as a simple means for determination of the embryonic HR.

Acoustic Stimulation↗