Biomedical subjects

H Tauchi

Publications and source records attributed to H Tauchi.

94 records · Page 6Linked to original sources

PubMedDate not supplied

Age and organ difference in amount and distribution of autofluorescent granules in rats.

The amount and distribution of autofluorescent granules in various organs and tissues of rats of different ages (2-, 11-, and 29.5-month-old) were compared by morphometrical analysis. The age-related increase of the granules was observed in the cardiac muscle and hepatic cells, but the amount of granules was little even in 29.5-month-old rats. In some other organs, the granules appeared early, at 2 months of age, increased up to 11 months, but thereafter no significant increase was observed. The autofluorescent granules are not merely considered to be an age-related pigment, but seems to be influenced by relationship to the cell metabolism and other functions.

Aging↗

PubMedDate not supplied

Age-related change in activation by Tris (hydroxymethyl) aminomethane on myosin-ATPase activity of human minor pectoral muscles.

Age related change of the human minor pectoral muscles was biochemically demonstrated. Myosin-ATPase activity was significantly decreased with age, and was activated with Tris. The degree of the activation by Tris was observed to be lower in old aged patients than in the young. Furthermore, at low concentration of CaCl2 (less than 100 microM), myosin-ATPase activity was higher in the young age than in the old, while at high concentration of CaCl2 (more than 1 mM) no significant difference was observed between young and old age. Decrease with age in activation by primary amine such as Tris would play an important role in the muscle working capacity in old age.

Adenosine Triphosphatases↗

PubMedDate not supplied

Comparative genomic hybridization analysis suggests a gain of chromosome 7p associated with lymph node metastasis in non-small cell lung cancer.

We analyzed the chromosomal gains and losses that occur in 30 non-small cell lung carcinomas by comparative genomic hybridization. Their chromosomal imbalances showed histological type-specific patterns in adenocarcinomas and in squamous cell carcinomas. The genetic changes in non-small cell lung carcinoma were also strongly dependent on metastasis to lymph node. The average numbers of chromosomal alterations were increased from 6.2 to 9.1 along with the presence of metastasis, and it gave rise to the increased copy number in specific chromosomes. In particular, a novel imbalance at 7p12-21 was recognized in a half of carcinoma with metastasis, although no genetic alteration was observed in 15 non-metastasizing lung carcinoma tested here.

Adenocarcinoma↗

PubMedDate not supplied

Absence of mutations in the NBS1 gene in B-cell malignant lymphoma patients.

BACKGROUND: Nijmegen breakage syndrome (NBS), also known as ataxia-telangiectasia (AT) variant, is an autosomal recessive disorder characterized by microcephaly, growth retardation, severe combined immunodeficiency and a high incidence of lymphoid carcinoma, the majority of which are B-cell lymphomas. To determine whether the NBS1 gene is a tumor suppressor gene in B-cell lymphoma, we screened B-cell malignant lymphoma (ML) for any evidence of NBS1 mutation. MATERIALS AND METHODS: Sequence analysis of the NBS1 gene was performed from PCR products amplified from the DNA of 12 extracranial ML or RT-PCR products amplified from cDNA of 8 primary central nervous system lymphoma. RESULTS: Direct sequence analysis revealed that no NBS1 mutations were present in any of these patients. CONCLUSION: The present results suggested that the contribution of NBS1 mutations to B-cell ML was minimal, despite the fact that the NBS1 gene was causative factor in these cases.

Adolescent↗