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Biomedical subjects

H Tasaka

Publications and source records attributed to H Tasaka.

At least 73 records · Page 4Linked to original sources
PubMed1987

Superoxide generation of leukemic cells in children.

Superoxide (O2-) generation of leukemic cells was investigated in 28 children with acute leukemia. Leukemic cells of seven with acute myelomonocytic leukemia and one with acute monocytic leukemia produced various amounts of O2- (0.13-0.87 nmol/min/10(6) cells) when stimulated with wheat germ agglutinin (WGA) and phorbol myristate acetate (PMA). Other types of leukemia cells exhibited no such functions. Leukemic cells of two patients diagnosed as unusual cases of acute lymphoblastic leukemia generated significant amounts of O2- when stimulated with WGA and PMA. Both patients went into complete remission while on acute nonlymphoblastic leukemia treatment. Thus O2- generating activity can serve as a functional marker of leukemic cells in myelomonocytic lineage.

Antigens, Neoplasm↗
PubMed1985

Specificity and distribution of alpha antigens of Mycobacterium avium-intracellulare, Mycobacterium scrofulaceum, and related species of mycobacteria.

Specificity of antigenic determinants in alpha antigens of Mycobacterium avium, M. intracellulare, M. scrofulaceum, M. gordonae, and M. szulgai was investigated by the agar gel diffusion technique using the respective absorbed antialpha serum on a total of 225 strains classified into 13 species of slowly growing mycobacteria. The specific antigenic determinants in alpha antigen of M. scrofulaceum, M. gordonae, and M. szulgai were species specific, whereas those of M. avium and M. intracellulare were M. avium-M. intracellulare complex specific. Alpha antigens were found to be stable. These results suggest that the absorbed serums of antialpha- M. avium-intracellulare, -M. scrofulaceum, -M. gordonae, and -M. szulgai are useful for serologic identification of these species of slowly growing mycobacteria.

Antigens, Bacterial↗
PubMed1984

Characterization of new non-T, non-B acute lymphoblastic leukemia cell lines: analysis of surface antigens by quantitative cellular radioimmunoassay and flow cytometry.

Two novel acute lymphoblastic leukemia (ALL) cell lines, HOON and HYON, have been established from patients with non-T, non-B ALL. The cell lines have been characterized and shown to express phenotypic markers on non-T, non-B ALL. By indirect immunofluorescence and flow cytometry they express Ia and common ALL (CALLA) antigens and are reactive with monoclonal antibodies BA-1, BA-2 and OKT-9. However, the cells do not express detectable amounts of B1 antigen or of cytoplasmic mu chain, which are markers of pre-B cells. Quantitation of Ia and CALLA antigens on HOON and HYON cell lines using a cellular radioimmunoassay revealed that both cells bind high levels of anti-Ia antibodies, 110 X 10(4) molecules per cell, and low levels of anti-CALLA antibodies, 7 X 10(4) molecules per cell. Although both HOON and HYON carry equivalent amounts of Ia on their surface, only the former is a good stimulator of the one-way mixed lymphocyte reaction.

Antigens, Neoplasm↗
PubMed1984

Specificity and distribution of alpha antigens of Mycobacterium kansasii and Mycobacterium marinum.

Specificity of antigenic determinants in alpha antigens of Mycobacterium kansasii and Mycobacterium marinum was investigated in 170 strains classified into 16 species of slowly growing mycobacteria by the agar gel diffusion technique using the respective absorbed anti-alpha serum. The specific antigenic determinants in alpha antigen of M. kansasii were detected in all of the 20 strains classified as M. kansasii, and not in strains belonging to other species. The specific antigenic determinants in alpha antigen of M. marinum were only detected in all of the 13 strains classified as M. marinum. These results suggest that the absorbed anti-alpha-M. kansasii and -M. marinum serums are useful for serologic identification of these species of slowly growing mycobacteria.

Antigens, Bacterial↗
PubMed1983

Purification and characterization of beta-glucuronidase inhibitor from Mycobacterium tuberculosis.

Factors inhibitory to beta-glucuronidase were found in the culture filtrate and in a bacillary extract of Mycobacterium tuberculosis H37Rv grown for 6 weeks on Sauton medium. The inhibitors were purified by ammonium sulfate fractionation, treatment with n-butanol and streptomycin, and chromatography on DEAE-Sepharose CL-6B. Two inhibitors were obtained from the culture filtrate. The molecular weights were estimated to be 25,500 and 15,500 by gel filtration on a Sephadex G-75 column. Three inhibitors were purified from the bacillary extract, two of which were similar to those from the culture filtrate. The molecular weight of the third inhibitor was 21,000. However, the molecular weight of all the denatured inhibitors was 8,600 in the presence of sodium dodecyl sulfate. The inhibitors contained extremely high amounts of glutamic and aspartic acids and had a highly acidic isoelectric point of pH 2.5. The inhibitors acted noncompetitively against beta-glucuronidase of guinea pig origin at an optimal pH 4.5. beta-Glucuronidases from human peripheral leukocytes and beef liver were partially sensitive to the inhibitors; all the other enzymes tested for sensitivity were unaffected by the inhibitors.

Amino Acids↗