Biomedical subjects

H Tao

Publications and source records attributed to H Tao.

At least 37 records · Page 2Linked to original sources

PubMed2000

[Experimental study on selective thrombolytic activity of pro-urokinase].

OBJECTIVE: To evaluate the valve of pro-urokinase as a selective thrombolytic agent in microsurgery. METHODS: The levators muscle model in rats was used to investigate the effect of pro-urokinase on dissolving ischemic thrombi and fibrinogenolytic activities in circulation by local infusion. The tissue microcirculation, pathologic appearance of rat lungs and muscles, and general fibrinogenolytic activities were observed. RESULTS: The thrombolytic rate of pro-urokinase was 92.3% (P < 0.05), the positive rate of D-dime was 83.3%(P < 0.05). No significant change of alpha 2-PI was observed on general fibrinogenolytic activities(P > 0.05). CONCLUSIONS: It is suggested that pro-urokinase can be used to dissolve the thrombi in microcirculation of free tissue transplantation without affect general fibrinogenolytic activities. It is safe to use pro-urokinase in free tissue transplantation.

Animals↗

PubMed2000

[Effect of foliar leaching on growth and mineral nutrition of maize under NaCl stress].

The impact of foliar leaching on growth and mineral nutrition maize under NaCl stress was investigated. The results showed that there was no difference in biomass between leaching and control treatments under low NaCl stress (0 and 50 mmol.L-1), but under high NaCl stress (100 and 200 mmol.L-1), the biomass of leaching treatment was higher, with a better at pH 3.5 than at pH 7.0. The shoot K+ content in leaching treatments was higher than that of control under no NaCl stress, but lower under 200 mmol.L-1 NaCl stress. Shoot Na+ content of leaching treatments was lower than that of control under high NaCl stress, and shoot Ca2+ and Mg2+ contents of leaching treatments were higher under no NaCl stress. Root K+, Na+, Ca2+ and Mg2+ content and relative water content of leaching treatments had no significant changes, compared with those of control. It was suggested that foliar leaching could alleviate harmful degree of maize under serious salt stress, which was related with the decrease of shoot Na+ content by foliar leaching.

Minerals↗

PubMed2000

Computer-aided 3-D reconstruction and measurement of the optic canal and intracanalicular structures.

OBJECTIVE: To reconstruct the human optic canal and its inner structures, and to provide detailed knowledge of this region for optic nerve decompression for further understanding on the pathologic mechanisms of indirect optic nerve injury. METHODS: Six optic canals and their inner structures were reconstructed using a computer-aided 3-dimensional reconstruction system. Quantitative measurement of the canal wall thickness, bony canal transverse area, optic nerve transverse area, dural sheath transverse area, subarachnoid space transverse area, and subarachnoid space volume were done by means of the computer morphometric analysis system. The detailed spatial relationship among intracanalicular structures were also carefully identified on the 3-D models. RESULTS: The thinnest portion of the canal was the middle part of the medial wall (0.45 +/- 0.35 mm) and the narrowest space was in the middle part of the optic canal (the transverse area was 18.21 +/- 2.50 mm2). The volume of subarachnoid space which can be considered the compensatory space for distention incurred by the hemorrhage, optic nerve edema, or hematoma was 21.16 +/- 4.31 mm3. At the cranial opening, the middle part and orbital opening, its transverse area was 4.45 +/- 1.12 mm2, 2.68 +/- 1.32 mm2 and 1.23 +/- 0.83 mm2, respectively. CONCLUSIONS: Since the compensatory space was limited, even a tiny amount of blood or swelling of the nerve may cause optic nerve compression. Because the narrowest space was in the middle part of the optic canal and the compensatory space for distention gradually decreases from cranial end to orbital end, the middle part and the anterior part of the optic canal and dural sheath are critical in optic nerve decompression.

Eye↗

PubMed2000

[Effect of ginsenosides on proliferation and differentiation of human CD34+ hematopoietic stem/progenitor cells].

OBJECTIVE: To investigate the effect of ginsenosides (GS) on proliferation and differentiation of human CD34+ stem/progenitor cells. METHODS: CD34+ hematopoietic progenitor cells were isolated from umbilical cord blood by using the immune beads sorting system. The cells were exposed to GS of different concentrations in both liquid culture and semi-solid culture, and the elevation rate on proliferation of CD34+ stem/progenitor cells and colony formation were estimated. The cells were marked with monoclonal antibody and the marker was examined by flow cytometry after incubated with GS for 14 days. RESULTS: GS (5-50 micrograms/ml) could raise the colony production rate of BFU-E, CFU-E, CFU-GM, CFU-GEMM by (87.6 +/- 2.6)%, (63.3 +/- 2.8)%, (58.0 +/- 3.1)% and (96.3 +/- 5.5)% respectively (all P < 0.01), and the best effect in improving cell proliferation of CD34+ cells in vitro was obtained when the concentration of GS was 25 micrograms/ml. After incubation with GS for 14 days, number of CD33+ cells was increased by GS in a dose-dependent manner with a peak increasing rate at 200 micrograms/ml. In the presence of GS 50 micrograms/ml, CD15+ cells were reaching the peak. Number of CD71+ and G-A+ cells increased only when the concentration of GS was 25 micrograms/ml. CONCLUSION: GS could not only promote the proliferation but also induce the differentiation of CD34+ hematopoietic stem/progenitor cells, GS may play the role by cooperating with hematopoietic growth factor, and by its growth factor-like function in the regulation of hematopoiesis.

Antigens, CD34↗

PubMed2000

[Relations of chemotherapy-induced tumor necrosis to plasma platin concentration and primary tumor temperature in patients with osteosarcoma in the lower extremities treated by hyperthermic isolation limb perfusion with cisplatin].

OBJECTIVES: To study the relations of chemotherapy-induced tumor necrosis to plasma concentration of platin and primary tumor temperature in hyperthermic isolation limb perfusion (HILP) with cisplatin, and to investigate the correlation between the tumor necrosis rate and the relapse-free survival (RFS). METHODS: Fifteen patients with II B osteosarcoma in the lower extremities were treated with neoadjuvant chemotherapy. Among them, 10 were male and 5 female. The average age was 18 years (ranging from 13 to 29). Chemotherapy included a single bonus dose of HILP with cisplatin (CDDP, 15 mg/m(2)). The plasma concentration of platin and the tumor temperature in the affected limb were measured during the course of the HILP with CDDP. Limb salvage surgery was performed in 9 patients and amputation in 6. After surgery, serial sections of the excised tumor specimens were made to determine the tumor necrosis rate. All of the patients were followed up for more than 5 years. RESULTS: The highest attained tumor temperature was between 41.4 degrees C - 43.0 degrees C (mean 42.3 degrees C +/- 0.46 degrees C), and the platin concentration ranged from 4.56 microg/mL to 14.66 microg/mL (mean 8.93 microg/mL +/- 3.16 microg/mL) during the course of HILP with CDDP. Thirteen patients with a necrosis rate of over 90% were well responded, and the remaining two with a necrosis rate 87.2% and 86.3% were poor responded. Eight patients remained tumor-free for 5 years. Primary tumor temperature and platin concentration were significantly related to the tumor necrosis rate (P = 0.001 and P < 0.001, respectively). No significant correlation was noted between the long-term survival rate and the three parameters in the regression analysis. CONCLUSIONS: HILP with CDDP is useful in the treatment of primary osteosarcoma. Increased tumor necrosis ensure a successful surgical excision of tumor. HILP however is still limited to the patients who are not candidates for lime salvage surgery, because the same condition of high temperature and high concentration of platin are not applicable to distant metastasis.

Adolescent↗

PubMed1999

Tin speciation in the femtogram range in open ocean seawater by gas chromatography/inductively coupled plasma mass spectrometry using a shield torch at normal plasma conditions.

A sensitive method for the determination of ultratrace organotin species in seawater is described. The merits and demerits of derivatization methods using Grignard reagent or sodium tetraethylborate (NaBEt4) were evaluated in terms of derivatization efficiency, applicability to the programmed temperature vaporization (PTV) method, and procedural blanks. The sensitivity of the gas chromatography/inductively coupled plasma mass spectrometry (GC/ICPMS) was improved by more than 100-fold by operating the shield torch at normal plasma conditions, compared with that obtained without using it. The absolute detection limit as tin reached subfemtogram (fg) levels. Furthermore, the detection limit in terms of relative concentration was improved 100-fold by using the PTV method, which enabled the injection of a large sample volume of as much as 100 microL without loss of analyte. When the organotin species in seawater were extracted into hexane with a preconcentration factor of 1000 after ethylation with NaBEt4 and a 100 microL aliquot of the extract was injected into the GC, the instrumental detection limit in relative concentration reached 0.01 pg/L in original seawater. Sources of contamination of organotin species during the sample preparation were examined, and a purification method of NaBEt4 was developed. Finally, the method was successfully applied to open ocean seawater samples containing organotin species at the level of 1-100 pg/L.

Borates↗

PubMed1999

Stage-specific expression of alpha1,2-fucosyltransferase and alpha1, 3-fucosyltransferase (FT) during mouse embryogenesis.

Lex [Galbeta1-4(Fucalpha1-3)GlcNAc] and Ley [Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc] are both stage-specific embryonic antigens. Lex is first detected on the blastomeres of the 8-cell stage embryo, which correlates with the onset of blastomere compaction. Ley is highly expressed on the surface of the blastocyst, which has been shown to be involved in blastocyst attachment in the mouse. In the present study, mouse alpha1,2-FT (also known as FUT1) and alpha1,3-FT (also known as Fuc-TIV), which were responsible for Lex and Ley formation, were examined in preimplantation stage embryos by reverse transcription-PCR and in situ hybridization. alpha1,3-FT mRNA was detected in all embryos of preimplantation stage, while alpha1,2-FT mRNA emerged in the later stage embryos from 8-cell to 16-cell to the blastocyst. These results indicated the expression of Ley was regulated by alpha1,2-FT. In situ hybridization showed that these two enzyme mRNAs were detected only in morula and blastocyst stage embryos. The alpha1,2-FT and alpha1, 3-FT mRNAs were located in both the inner cell mass and the trophoblast cells. 2-Cell and 4-cell embryos were isolated from the oviduct and cultured in vitro to the 8-cell, morula and blastocyst stage. The expression of alpha1,2-FT and alpha1,3-FT were observed in these embryos developed in vitro; immunohistochemical analysis also showed that Ley expression was positive. These results suggested the stage-specific expression of Ley on the embryos was synthesized by endogenous alpha1,2-FT and alpha1,3-FT rather than transfer from other sources. In addition, the expression of alpha1, 2-FT was differentially regulated and the uterine factor was not prerequisite of the expression of Ley.

Animals↗

PubMed1999

Cord blood is better than bone marrow for generating megakaryocytic progenitor cells.

Thrombocytopenia remains an important problem for patients post high-dose chemotherapy and hematopoietic stem cell transplantation. The study of megakaryocytes, the direct precursors of platelets, has been hampered by their relatively low frequency in hematopoietic tissues. In an attempt to obtain a large number of functional megakaryocytic cells, we established a serum-free culture system to grow megakaryocytic progenitor cells derived from normal human bone marrow (BM) and cord blood (CB). Highly purified (purity >95%) CD34+ cells were obtained using magnetic cell sorting (MACS) followed by fluorescence activated cell sorting (FACS). The cells were cultured in a serum-free culture system for 3 weeks in the presence of a single dose of MGDF (50 ng/mL). On days 0, 5, 8, 12, 14, 18, and 21 of culture, the cellularity and morphology were examined. Megakaryocytic cells were monitored by detecting the expression of GPIIIa (CD61), GPIIb/IIIa (CD41) and GPIb (CD42b), and the distribution of megakaryocyte (MK) ploidy was analyzed by two-color flow cytometry. MGDF alone induced maximal nucleated cell expansion at day 14, resulting in a 38.20+/-10.47-fold increase in cell number for CB and a 5.08+/-1.30-fold increase in cell number for BM. On day 14 of the culture, the percentage of CD41-/CD14- cells derived from CB reached 73.54%+/-6.01% giving an absolute number of CD41+/CD14- cells of 27.25+/-2.23 x 10(4)/mL (27,250-fold increase), whilst the percentage of CD41+/CD14- cells derived from BM was only 29.21%+/-5.63% with an absolute number of 1.36+/-0.26 x 10(4)/mL (680-fold increase). Increased expression of GPIIIa occurred the earliest in culture, followed by GPIIb/IIIa, and then GPIb. The majority (81.6%-92.6%) of megakaryocytes (CD41+ cells) on day 14 of culture were 2N, although we did detect some 4N, 8N and greater ploidy cells. In conclusion, CD34+ cells stimulated by MGDF alone generated highly enriched MK progenitor cells at day 14 of serum-free culture. CB stem and progenitor cells have a greater proliferative response to MGDF alone than those derived from BM and may, therefore, prove to be a better source of cells for MK expansion.

Cell Differentiation↗

PubMed1999

Reciprocal regulation of gadd45 by C/EBP alpha and c-Myc.

The CCAAT/enhancer-binding protein-alpha (C/EBP alpha) drives the differentiation of murine 3T3-L1 cells to adipocytes through transcriptional activation of phenotype-associated genes via proximal promoter elements. In addition, C/EBP alpha suppresses mitotic growth. We report here that C/EBP alpha directly regulates gadd45 through a C/EBP-binding site in the proximal promoter. A 3 basepair substitution, directed at the most conserved residues of the sequence, reduced C/EBP alpha-mediated transactivation and impaired binding of C/EBP alpha in adipocyte nuclear extracts. We also found that c-Myc antagonized C/EBP alpha-mediated transactivation of gadd45. Analysis of systematically altered forms of C/EBP alpha revealed that c-Myc antagonism targeted the antimitotic, transcriptional activation domain of C/EBP alpha. In addition, we localized the regulatory sequences in the gadd45 promoter that are required for c-Myc antagonism of C/EBP alpha transactivation. Our findings reveal that C/EBP alpha coordinates cellular differentiation and mitotic growth arrest through direct, coordinate regulation of phenotype-associated and growth-arrest-associated genes. In addition, our findings reveal that the reciprocal relation between C/EBP alpha and c-Myc in 3T3-L1 cells includes antagonistic transcriptional control of a growth-arrest-associated gene.

3T3 Cells↗

PubMed1999

Computer-aided three-dimensional reconstruction and measurement of the optic canal and intracanalicular structures.

OBJECTIVE: To reconstruct the human optic canal and its inner structures and to provide detailed knowledge of this region for optic nerve decompression. METHODS: Six optic canals and their inner structures were reconstructed using a computer-aided three-dimensional reconstruction system. Quantitative measurement of the canal wall thickness, bony canal transverse area, optic nerve transverse area, dural sheath transverse area, subarachnoid space transverse area, and subarachnoid space volume was done using the computer morphometric analysis system. The detailed spatial relationship among intracanalicular structures was also carefully identified on the three-dimensional models. RESULTS: The thinnest portion of the canal was the middle part of the medial wall (0.45 +/- 0.14 mm) and the narrowest space was in the middle part of the optic canal (the transverse area was 18.21 +/- 1.20 mm2). The volume of subarachnoid space that can be considered the compensatory space for distention incurred by the hemorrhage, optic nerve edema, or hematoma was 21.16 +/- 4.31 mm3. At the cranial opening, the middle part, and the orbital opening, its transverse area was 4.45 +/- 0.46 mm2, 2.68 +/- 0.54 mm2, and 1.23 +/- 0.34 mm2 respectively. CONCLUSIONS: Because the compensatory space was limited, even a tiny amount of blood or swelling of the nerve may cause optic nerve compression. Because the compensatory space for distention gradually decreases from cranial end to orbital end, the middle part and the anterior part of the optic canal and dural sheath are critical in optic nerve decompression.

Adult↗

PubMed1999

An estimation method for the semiparametric mixed effects model.

A semiparametric mixed effects regression model is proposed for the analysis of clustered or longitudinal data with continuous, ordinal, or binary outcome. The common assumption of Gaussian random effects is relaxed by using a predictive recursion method (Newton and Zhang, 1999) to provide a nonparametric smooth density estimate. A new strategy is introduced to accelerate the algorithm. Parameter estimates are obtained by maximizing the marginal profile likelihood by Powell's conjugate direction search method. Monte Carlo results are presented to show that the method can improve the mean squared error of the fixed effects estimators when the random effects distribution is not Gaussian. The usefulness of visualizing the random effects density itself is illustrated in the analysis of data from the Wisconsin Sleep Survey. The proposed estimation procedure is computationally feasible for quite large data sets.

Biometry↗

PubMed1999

Functional genomics: expression analysis of Escherichia coli growing on minimal and rich media.

DNA arrays of the entire set of Escherichia coli genes were used to measure the genomic expression patterns of cells growing in late logarithmic phase on minimal glucose medium and on Luria broth containing glucose. Ratios of the transcript levels for all 4,290 E. coli protein-encoding genes (cds) were obtained, and analysis of the expression ratio data indicated that the physiological state of the cells under the two growth conditions could be ascertained. The cells in the rich medium grew faster, and expression of the majority of the translation apparatus genes was significantly elevated under this growth condition, consistent with known patterns of growth rate-dependent regulation and increased rate of protein synthesis in rapidly growing cells. The cells grown on minimal medium showed significantly elevated expression of many genes involved in biosynthesis of building blocks, most notably the amino acid biosynthetic pathways. Nearly half of the known RpoS-dependent genes were expressed at significantly higher levels in minimal medium than in rich medium, and rpoS expression was similarly elevated. The role of RpoS regulation in these logarithmic phase cells was suggested by the functions of the RpoS dependent genes that were induced. The hallmark features of E. coli cells growing on glucose minimal medium appeared to be the formation and excretion of acetate, metabolism of the acetate, and protection of the cells from acid stress. A hypothesis invoking RpoS and UspA (universal stress protein, also significantly elevated in minimal glucose medium) as playing a role in coordinating these various aspects and consequences of glucose and acetate metabolism was generated. This experiment demonstrates that genomic expression assays can be applied in a meaningful way to the study of whole-bacterial-cell physiology for the generation of hypotheses and as a guide for more detailed studies of particular genes of interest.

Amino Acids↗

PubMed1999

[Effect of anti-vascular endothelial growth factor antibody on the progression of human gastric cancer orthotopic xenotransplanted into nude mice].

OBJECTIVE: To study the inhibiting effect of anti-vascular endothelial growth factor (VEGF) on the growth and metastasis of gastric cancer. METHODS: The anti-tumor and anti-metastasis effect of anti-VEGF antibody, mitomycin C (MMC) were investigated by means of an orthotopic xenotransplanted model of human gastric cancer SGC-7901 in nude mice which had been randomly divided into 4 groups: control group receiving PBS, group receiving 50 microg/mice anti-VEGF antibody, group receiving 2 mg/kg MMC, and group receiving 50 microg/mice anti-VEGF antibody combined with 2 mg/kg MMC. Anti-VEGF antibody was given i.p. twice a week and MMC was administered i.p. once a week for 8 weeks from day 7 after transplantation. All animals were sacrificed at the end of 10 weeks. The tumor was weighted and the intra-tumoral microvessel density (MVD) was recorded, and the liver was histologically examined in order to discover micrometastasis. RESULTS: Both anti-VEGF antibody and MMC showed a significant inhibitory effect on the growth of primary tumors; in the combination treatment group the inhibitory effect was more significant than single agent. Liver metastasis developed in 19 of 28 mice (67.9%) of the controls and in 6 of 11 mice (54.5%) receiving MMC. In contrast, liver metastasis occurred in 2 of 10 mice (20%) receiving anti-VEGF antibody and none receiving combination treatment. In addition, the MVD was less significant in the anti-VEGF antibody group and combination treatment group than other groups. CONCLUSIONS: Anti-VEGF may provide a new approach to the treatment of gastric cancer by inhibiting tumor angiogenesis, and combination of anti-VEGF antibody with MMC could be more effective.

Animals↗

PubMed1999

[Computer-aided 3-D reconstruction and measurement of optic canal and intracanalicular structures].

OBJECTIVE: To reconstruct the human optic canal and its inner structures, and to provide knowledge of this region in detail for optic nerve decompression and further understanding on the pathologic mechanisms of indirect optic nerve injury. METHODS: 6 optic canals and its inner structures were reconstructed by using a computer-aided 3-dimensional (3-D) reconstruction system. Quantitative measurement for the canal wall thickness, bony canal transverse area, optic nerve transverse area, dual sheath transverse area, subarachnoid space transverse area, and subarachnoid space volume were done by means of the computer morphometric analysis system. The detailed spatial relations among intracanalicular structures were also carefully identified on the 3-D models. RESULTS: The thinnest portion of the canal was the middle part of the medial wall (0.45 +/- 0.35)mm, and the narrowest space was in the middle part of the optic canal [the transverse area was (18.21 +/- 2.50) mm(2)]. The volume of subarachnoid space which can be considered the compensatory space for distention incurred by the hemorrhage, optic nerve edema, or hematoma was (21.16 +/- 4.31) mm(3). At the cranial opening, the middle part and orbital opening, its transverse area was (4.45 +/- 1.12) mm(2), (2.68 +/- 1.32) mm(2), (1.23 +/- 0.83) mm(2), respectively. CONCLUSIONS: In the middle part, the restraining of the bony canal on injured nerve may be the most powerful. Since the compensatory space was limited, even a tiny amount of blood or swelling of the nerve may cause optic compression. Because the compensatory space for distention gradually decreases from cranial end to orbital end, the middle part and the anterior part of the optic canal and dural sheath are critical in optic nerve decompression.

Humans↗

PubMed1999

[First discovery of Taenia saginata asiatica infection in Yunnan province].

AIM: To investigate the aetiology, species and epidemiological factors of Taenia infection in a pilot area of Lanping County, Yunnan Province. METHODS: Two patients with taeniasis were treated with antiscolex capsule and praziquantel, respectively. Five local weaning pigs free from tapeworm infection were fed with gravid proglottids obtained from the patients. After 2-3 months, the pigs were necropsied to examine cysticerci. In addition, one pig naturally infected with Taenia was treated as well. RESULTS: Based on morphological characteristics of the scolex and gravid proglottid of four tapeworms obtained from the patients, the worms were similar to T. saginata. 23 cysticerci recovered from two experimentally infected pigs were found in the livers, omentum majus and mesenterium, and 3 cysticerci were found in the liver and omentum majus in a naturally infected pig as well. The protoscolex of mature Cysticercus had two rows of rudimentary hooklets, one rostellum and four acetabula resembling to C. cellulosae. According to morphological characteristics and the location of cysticerci, the tapeworm was identified as Taenia saginata asiatia. CONCLUSION: T. saginata asiatica infection was first reported in Yunnan Province.

Adult↗

PubMed1998

Gene therapy in allergic encephalomyelitis using myelin basic protein-specific T cells engineered to express latent transforming growth factor-beta1.

A myelin basic protein (MBP)-specific BALB/c T helper 1 (Th1) clone was transduced with cDNA for murine latent transforming growth factor-beta1 (TGF-beta1) by coculture with fibroblasts producing a genetically engineered retrovirus. When SJL x BALB/c F1 mice, immunized 12-15 days earlier with proteolipid protein in complete Freund's adjuvant, were injected with 3 x 10(6) cells from MBP-activated untransduced cloned Th1 cells, the severity of experimental allergic encephalomyelitis (EAE) was slightly increased. In contrast, MBP-activated (but not resting) latent TGF-beta1-transduced T cells significantly delayed and ameliorated EAE development. This protective effect was negated by simultaneously injected anti-TGF-beta1. The transduced cells secreted 2-4 ng/ml of latent TGF-beta1 into their culture medium, whereas control cells secreted barely detectable amounts. mRNA profiles for tumor necrosis factor, lymphotoxin, and interferon-gamma were similar before and after transduction; interleukin-4 and -10 were absent. TGF-beta1-transduced and antigen-activated BALB/c Th1 clones, specific for hemocyanin or ovalbumin, did not ameliorate EAE. Spinal cords from mice, taken 12 days after receiving TGF-beta1-transduced, antigen-activated cells, contained detectable amounts of TGF-beta1 cDNA. We conclude that latent TGF-beta1-transduced, self-reactive T cell clones may be useful in the therapy of autoimmune diseases.

Animals↗

PubMed1998

[Prognostic value of tumor vascularity in gastric carcinoma].

OBJECTIVE: To determine whether tumor angiogenesis correlates with progression of gastric carcinoma. METHOD: Microvessel counts (MVC) of 131 specimens resected from patients with gastric carcinoma were investigated by immunohistological staining with a monoclonal antibody against F-VIII RAg, counting the mean number of microvessels in the five areas of highest vascular density under 200 times magnification microscopy. Correlation among MVC, various clinicopathologic factors, and prognosis was studied. RESULT: MVC increased with TNM stage, the MVC of patients with advanced stage disease was significantly higher than that of early stage patients (P < 0.01). MVC was significantly higher in tumors with deep penetration (P < 0.05), lymph node metastasis (P < 0.001) and distant metastasis (P < 0.05) than in those without invasion and such metastasis. The recurrence rate after curative resection in hypervascular group (MVC > or 16) was 58.4%, which was significantly higher (P < 0.05) than that in the hypovascular group (MVC < 16). Moreover, the prognosis of patients with a high MVC was significantly poorer than that of those with a low MVC. The probability of 5 year survival rate was estimated by P = e(1.6231-0.1036 x MVC)/1 + e(1.6231-0.1036 x MVC). Multivariate analysis indicated that MVC is an independent prognostic factor in gastric cancer. CONCLUSION: Angiogenesis within gastric cancer is an indicator of tumor behavior and may identify patients at higher risk for recurrence and poorer prognosis.

Adult↗

PubMed1998

[Study on the design and application of the contact Nd:YAG laser scalper].

A method of designing the contact laser scalper is presented. The calculations using this method is in good agreement with the measured results in laboratory. Some operations on rabbits using the newly developed contact Nd:YAG laser scalper are performed and shown that the contact Nd:YAG laser is a promising medical equipment.

Aluminum Oxide↗