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Biomedical subjects

H Tamura

Publications and source records attributed to H Tamura.

At least 415 records · Page 23Linked to original sources
PubMed1987

Role of luteinizing hormone in luteotropic complex of pregnant hamster.

Hamsters were hypophysectomized on day 4 of pregnancy (day 1 = sperm in vaginal smear) and injected subcutaneously on days 4-7 with various combinations of 200 micrograms prolactin (Prl), 10 micrograms follicle-stimulating hormone (FSH), and 20 micrograms luteinizing hormone (LH) in polyvinylpyrrolidone (PVP) to decrease its rate of absorption or in saline. End points for luteal function on day 8 were maintenance of pregnancy, serum progesterone (P4), luteal weight, and luteal binding for human chorionic gonadotropin, FSH, and Prl. After hypophysectomy, a drastic decline occurred in all parameters including an 89% decrease in luteal weight. Injection of Prl did not maintain pregnancy nor serum P4 but partially maintained luteal weight and human chorionic gonadotropin binding sites per corpus luteum. The minimal luteotropic complex of Prl and FSH was effective in maintaining pregnancy and significantly increased serum P4 and Prl and FSH receptors but not to control levels; Prl and LH (PVP) was also effective to the same extent. Antral follicles were lacking after either treatment. The effects of FSH cannot be attributed to LH contamination. All variables were restored to control levels by Prl plus FSH plus LH (PVP) and antral follicles were present; Prl plus FSH plus LH (saline), however, induced luteolysis and reduced most values to the levels found in untreated, hypophysectomized animals. Thus, the luteotropic activity of LH was only demonstrable when it was injected in a long-acting form; when delivered as a bolus, LH (saline) was luteolytic.

Animals↗
PubMed1987

[Method of separating mouse platelets by discontinuous sucrose-gradient centrifugation].

A discontinuous sucrose gradient was employed in the separation of mouse blood platelets using a modified Booyse method. The platelets of male CD-1 mice aged 8 to 12 weeks were divided into five distinct populations (A, B, C, D & E). Distribution of light to heavy platelets patterns in 10 normal CD-1 mice was demonstrable at; A (S.G. 1.188), as 14.8 +/- 5.6%; B (S.G. 1.199), 44.0 +/- 4.6%; C (S.G. 1.207), 24.1 +/- 3.4%; D (S.G. 1.214), 13.0 +/- 3.6%; and E (S.G. 1.221), 4.0 +/- 1.5%.

Animals↗
PubMed1987

Quantitative measurement of endotoxin in canine plasma using the new endotoxin-specific chromogenic test.

Endospecy (a lyophilized mixture of factor G-free limulus coagulation enzymes and chromogenic substrate, Boc-Leu-Gly-Arg-pNA) coupled with modified perchloric acid (PCA) pretreatment was carried out for the quantitative measurement of endotoxin in canine plasma. The endotoxin recovery from normal canine plasma was 99.9 +/- 7.7% (n = 20). The full recovery of endotoxin illustrated the applicability of the modified PCA pretreatment to the Endospecy in removal of interfering factors in a canine plasma. The normal canine plasma endotoxin level was less than 3.0 pg.ml-1 when Escherichia coli 0111:B4 endotoxin was used as a reference. The canine plasma endotoxin levels were markedly high(1-40 ng.ml-1) at 5 min after intravenous administration of 25 micrograms.Kg-1 (total 150-200 micrograms).

Animals↗
PubMed1987

Alcuronium pretreatment attenuates succinylcholine-induced increases in plasma catecholamine concentrations in humans.

The effect of alcuronium given before succinylcholine (SCh) on plasma catecholamine concentrations, systolic blood pressure and heart rate was studied in patients anesthetized with enflurane. Twenty-one patients were divided into three groups; six control patients without SCh, eight SCh (1 mg/kg) patients, and seven pretreated patients given alcuronium, 0.04 mg/kg, 5 min before SCh, 1 mg/kg. In the SCh group, mean plasma norepinephrine concentrations, systolic blood pressure, and heart rate significantly increased with onset of fasciculations, whereas in the pretreated patients these variables did not change significantly and no fasciculation was observed. These results indicate that alcuronium pretreatment significantly attenuates the SCh-induced increases in plasma norepinephrine concentrations, systolic blood pressure and heart rate.

Adult↗