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Biomedical subjects

H Takagi

Publications and source records attributed to H Takagi.

At least 1,117 records · Page 62Linked to original sources

Efficacy of morantel tartrate in a sustained release bolus on the control of subclinical gastrointestinal parasitism in first-year grazing dairy replacements.

Thirty-four heifers (24 Holstein, 8 Ayrshire, 2 Jersey) and 12 Holstein steer calves were grouped into 23 blocks of 2 animals each according to breed, sex, and body weight. Animals in each block were randomly assigned to control or treated groups. The treated group received an anthelmintic bolus containing morantel tartrate before they were permitted access to pasture. A 2.6 ha pasture was divided in half with a double fence; the resulting two pastures had separate feed and water troughs. Steer calves were slaughtered upon termination of the trial to ascertain gastrointestinal parasite burdens. Two worm-free tracer calves were placed on each pasture every 2 mo and slaughtered after 4 wk of grazing to determine parasite infectivity of pastures. Herbage samples were obtained monthly and analyzed for infective larvae. The trial was conducted for 141 d during the grazing season. Treatment resulted in 90% reduction in infective larvae on pasture, 74% reduction in fecal worm-eggs, 91% reduction in adult worm burdens, and a trend toward reduced worm burdens in tracer calves by 52%. An overall average daily gain of .68 and .88 kg was obtained for control and treated animals. Reproductive data for the heifers remaining in the herd from the trial showed that treatment resulted in 44 less days to first breeding.

Animals↗

Determination of the complete nucleotide sequence of the Brevibacterium lactofermentum plasmid pAM330 and the analysis of its genetic information.

The complete nucleotide sequence of the plasmid pAM330 derived from Brevibacterium lactofermentum ATCC 13869 was determined using the dideoxy chain termination method. Analysis of the sequence allowed us to observe seven open reading frames, one of which showed the presence of the promoter and the Shine-Dalgarno (SD) sequences upstream from the initiation codon.

Base Sequence↗

[Histopathological study of the parathyroid gland in renal hyperparathyroidism].

From July 1973 to July 1984, 50 patients underwent parathyroidectomy because of secondary hyperparathyroidism due to chronic renal failure. One hundred fifty-eight excised parathyroid glands in 41 of these patients were submitted to the histopathological examinations in the relations to their clinical features which revealed the following findings. Inspite of secondary hyperparathyroidism, eleven of 41 patients showed four unevenly enlarged parathyroid glands with one or two glands weighing less than 100mg. The glands were divided into three adenomas, 101 diffusely and 54 nodulary hyperplastic glands. The nodular type hyperplasia, which had a greater proportion of clear chief cells, vacuolated chief cells, transitional oxyphil cells and oxyphil cells, was more hyperplastic than the diffuse type. The patients with the nodular type showed more advanced clinical features in the secondary hyperparathyroidism.

Adult↗

Fine structure of histaminergic neurons in the caudal magnocellular nucleus of the rat as demonstrated by immunocytochemistry using histidine decarboxylase as a marker.

The morphology of histamine-containing neurons in the caudal magnocellular nucleus was light and electron microscopically examined by means of peroxidase-antiperoxidase (PAP) immunocytochemistry with histidine decarboxylase (HDC) as a marker. HDC-like immunoreactive (HDCI) neurons had large (25-30 microns in diameter) perikarya from which two to four primary dendrites arose. The perikarya had a nearly round nucleus and well-developed Golgi apparatus in addition to a large number of mitochondria and rough endoplasmic reticulum. Immunoreactive endproducts were found diffusely throughout the perikarya, dendrites, and axons. HDCI neurons made synaptic contact with nonreactive axon terminals on the perikarya and dendrites. In addition, the HDCI neurons very frequently formed puncta adherentia with neuronal elements, either HDCI or nonreactive, or glial cells. Most of the HDCI axon terminals serially observed under electron microscopy did not exhibit typical synaptic contact in the caudal magnocellular nucleus. These findings suggest the nonsynaptic release of histamine in the caudal magnocellular nucleus.

Animals↗

Fine structural studies of cholecystokinin-8-like immunoreactive neurons and axon terminals in the nucleus of tractus solitarius of the rat.

Cholecystokinin (CCK)-8-like immunoreactive structures in the nucleus of tractus solitarius (NTS) were studied by using the peroxidase-antiperoxidase (PAP) immunohistochemical method. Immunoreactivity was localized in cell bodies and nerve fibers. The perikarya were oval or fusiform (average length 13 micron) and were mostly located in the dorsal half of the medial subnucleus of the NTS at the level of the area postrema (AP). One to three straight immunoreactive dendritelike processes emerged from the perikarya. Neurons that had first been identified under light microscopy were also studied by electron microscopy. Each neuron had a moderate amount of cytoplasm and an oval or elongated nucleus that was eccentrically located in the soma. A few synaptic inputs were found on the CCK immunoreactive perikarya, while a moderate number were seen on both proximal and distal dendrites. These neurons received both asymmetrical and symmetrical synaptic inputs. The immunoreactive dendrites were most frequently in asymmetrical synaptic contact with nonreactive boutons (max. 2.7 micron in diameter) containing fairly densely packed, small round vesicles. CCK immunoreactive boutons located in the NTS at the level of the AP were analyzed using electron microscopy; these boutons formed asymmetrical synaptic contact with other neuronal elements. Their postsynaptic targets were immunoreactive and nonreactive perikarya and dendrites. These data suggest that CCK-containing afferents might affect the neurotransmission of heterogenous types of solitary neurons.

Animals↗

Immunoelectron-microscopic study on the fine structure of substance-P-containing fibers in the taste buds of the rat.

The fine structure of substance-P-like immunoreactive [SPI] fibers in the taste buds of the circumvallate papillae of the rat tongue was investigated by means of electron microscopy using the unlabeled antibody-enzyme method. Outside the epithelium, SPI and non-SPI fibers are surrounded by the cytoplasm of Schwann cells. When the SPI fibers enter the epithelium, they immediately lose this cytoplasmic sheath and begin to traverse the taste buds. Though passing through the taste buds, no profiles suggesting clear synaptic contact between SPI fibers and underlying cells are identified. SPI terminals are filled with small synaptic vesicles and contain a few mitochondria. No SPI-positive structures are found in nerve endings that make synaptic contact with type III cells, the gustatory receptor cells.

Animals↗

Stimulation of DNA-mediated transformation by UV irradiation of recipient (mouse FM3A) cells.

DNA-mediated transformation for thymidine autotrophs (Tk+) was stimulated severalfold when the recipient (mouse FM3A) (Tk-) cells were preirradiated by UV light. The effect was most prominently seen with the cells irradiated 12-16 hours prior to DNA addition. A similar stimulatory effect was observed when the cells were treated with novobiocin or mitomycin C. The effect, however, was blocked when cycloheximide was present during postirradiation period, suggesting that de novo protein synthesis is required for producing the effect. Most of the Tk+ transformants arised from the UV-irradiated cells carried the donor DNA sequences in their chromosomes.

Animals↗

Near-total gastrectomy.

Total gastrectomy has more frequent and serious postoperative complications than subtotal gastrectomy. An esophago-gastric stoma with a side-to-side anastomosis permits a near-total gastrectomy to be undertaken with virtually the same results as a total gastrectomy. This procedure is used for early gastric cancers that have widely spreading, single or multiple lesions, or lesions near the esophago-gastric junction, and for the cure or palliative resection of selected patients with advanced gastric cancer.

Gastrectomy↗