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Biomedical subjects

H Takagi

Publications and source records attributed to H Takagi.

At least 595 records · Page 33Linked to original sources
PubMed1994

Extremely high levels of CA19-9 and CA125 antigen in benign mucinous ovarian cystadenoma.

A Japanese woman had a mucinous cystadenoma of the left ovary associated with very high serum levels of CA19-9 (3170 u/ml) and CA125 (1705 u/ml). Such high levels of both antigens have not been reported previously in patients with benign ovarian mucinous cystadenoma. Immunostaining demonstrated CA19-9 and CA125 in the tumor, and different patterns of expression were noted depending on the differentiation of the epithelium. The postoperative tumor marker profile suggested that no residual tumor was left after the operation.

Aged↗
PubMed1994

Pharmacokinetics and pharmacodynamics of serum glycerol in patients with brain edema: comparison of oral and intravenous administration.

To clarify the pharmacokinetics and effect of ICP reduction of glycerol administered orally, the serum concentration of glycerol was measured by the enzymatic method and ICP was measured by the subdural balloon method in severe head injured patients with brain edema and increased ICP. Sequential change of serum glycerol concentration and its relationship to the reduction of ICP were analyzed. The results showed that the pharmacokinetics of glycerol through oral administration were similar to that of intravenous glycerol administration and the changes of ICP were also similar to that of intravenous glycerol administration. We determined that glycerol can be administered either per oral or per venous to obtain the same results for treatment of brain edema with raised ICP.

Administration, Oral↗
PubMed1994

Expression of glutathione-S-transferases alpha and pi in gastric cancer: a correlation with cisplatin resistance.

Glutathione-S-transferase (GST) in one of several factors that are proposed to affect tumor sensitivity to anticancer drugs, including cisplatin (CDDP). Attempts are made herein to evaluate the significance of the enzymes in resistance to CDDP in clinical samples of gastric cancer. A total of 22 gastric cancer specimens, 16 of which were obtained with matching normal mucosae, underwent immunoblotting with polyclonal antibodies against GST-alpha and GST-pi. At the same time, the chemosensitivity of 15 gastric cancer specimens to CDDP was evaluated by the succinic dehydrogenase inhibition (SDI) test. The expression of GST-pi was detected in all the specimens, and its content in the neoplasms exhibited a significant positive correlation with that in the matched normal mucosae. The expression of GST-alpha was detected in 18 of 22 cancer specimens (82%), but its content in the neoplasms did not correlate with that in the matched mucosae. A comparison of the drug-sensitivity findings with the results of immunoblotting revealed a weak but interesting correlation between the protein levels of GST-alpha and CDDP resistance. The cellular content of GST-alpha correlated weakly with CDDP resistance in gastric cancer, and its quantification could contribute to prediction of the clinical effects of CDDP in patients with gastric cancer.

Cisplatin↗
PubMed1994

Effects of hepatic arterial infusion chemotherapy on unresectable or recurrent hepatocellular carcinoma.

We performed hepatic arterial infusion chemotherapy (HAI) on 86 patients with unresectable hepatocellular carcinoma (HCC, 61 patients) or unresectable recurrent HCC after hepatectomy (25 patients). As drug therapy, 250 mg of 5-fluorouracil was injected daily for 14 days using a reservoir embedded in the subcutaneous layer. During this period, 0.4 mg/kg of doxorubicin and 0.12 mg/kg of mitomycin C suspended in Lipiodol Ultra-Fluide were also injected twice intra-arterially. This was defined as one course of HAI, and it was repeated every 3 months. In the patients with unresectable HCC, the 1-, 2-, and 3-year survival rates were 31.5%, 22.4%, and 10.7%, respectively, and the numbers of cases showing a complete response (CR), a partial response (PR), a minor response (MR), no change (NC), and progressive disease (PD) according to the Criteria for the Evaluation of the Clinical Effects of Solid Cancer Chemotherapy established by the Japan Society for Cancer Therapy were 1 (1.6%), 20 (32.8%), 5 (8.2%), 28 (45.9%), and 7 (11.5%), respectively. On the other hand, the 1-, 2-, and 3-year survival rates of the patients with unresectable recurrent HCC were 69.6%, 34.8%, and 14.9%, respectively. The rate of catheter patency after 1 year was 64.1%, and the mean catheter-patency period was 311.9 days. Patients in group A (CR+PR, n = 21) survived significantly longer than those in group B (MR+NC+PD, n = 40; P < 0.05). In conclusion, since responders to HAI achieve longer survival than nonresponders, the selection of effective drugs is important for this therapy.

Adult↗
PubMed1994

Experimental study on chemotherapy using adsorbent charcoal with CDDP administered directly into the mediastinum following esophagectomy.

In this study, 1500AA, an activated charcoal with a strong affinity for the lymphatic system, was examined for its ability to deliver chemotherapy to metastatic lymph nodes. First, the anticancer effect on metastatic lymph nodes was assessed in an experiment using the mouse mammary tumor, MM48. Pathological examination of the inguinal lymph nodes revealed a metastatic rate of 50% in the group given cisplatin (CDDP)-saline, but 20% in the group given CDDP-charcoal. Next, in a canine model, CDDP dissolved in normal saline was administered directly into the mediastinum, and CDDP was given with fine particles of activated charcoal in the same manner in the other group. The concentration of CDDP in the lymph nodes and the plasma rapidly increased to peak in 10 min in the CDDP-saline group, whereas it increased slowly to peak in 20-30 min in the CDDP-charcoal group. Moreover, the CDDP-charcoal group was found to have a much higher concentration of CDDP in the lymph nodes than the CDDP-saline group.

Adenocarcinoma↗
PubMed1994

Pelvic actinomycosis presenting with a rectal stricture: report of a case.

We present herein the case of a patient with actinomycosis involving the pelvic cavity, who presented with a rectal stricture. It is rare for this type of actinomycosis to be accurately diagnosed preoperatively, but a precise diagnosis, made using needle biopsy guided by computed tomography allowed for successful treatment by resection of the granulation tissue surrounding the rectal stricture through a transsacral approach.

Actinomycosis↗
PubMed1994

Experimental rat pancreas transplant: surgical technique and immunological considerations.

Vascularized whole pancreas transplantation in the rat was performed on the abdomen using a cuff technique for vascular anastomoses. Two different exocrine drainage procedures, either intestinal or ureter drainage, were used. In the isograft transplant models, hyperglycemia was ameliorated immediately after transplantation and all of the grafts functioned during the observation period. In the allograft transplant models without immunosuppression, graft rejection, as defined by recurrence of hyperglycemia (blood glucose > 200 mg/dl) occurred 6-9 days post-transplant. Allograft rejection could be delayed approximately 1 month after transplant with short-term use of FK506. These different models, using either intestinal or ureter exocrine drainage, are similar to dominant clinical pancreas transplantation with enteric exocrine drainage or urinary tract drainage, respectively. It is thus concluded that whole pancreas transplant with either intestinal or ureter exocrine drainage is an ideal model for physiological and immunological experimental studies in pancreas transplantation.

Animals↗
PubMed1994

Elevation of N1-acetylspermidine and putrescine in hepatic tissues of patients with fulminant hepatitis and liver cirrhosis.

Hepatic polyamines were assayed in 15 patients with liver diseases, 5 with fulminant hepatitis (FH); 3 with exacerbated liver cirrhosis (ELC); and 7 with liver cirrhosis (LC). Hepatic putrescine and N1-acetylspermidine as percentages of total polyamines, were elevated and spermine was decreased in all 15 patients. The increase in hepatic N1-acetylspermidine levels appeared to be greater in patients with FH and ELC than in those with LC. These results suggest that the production of N1-acetylspermidine in human liver is closely associated with the induction of spermidine/spermine N1-acetyltransferase activity in liver diseases.

Acetyltransferases↗
PubMed1994

Measurements of the skull base for transpetrosal surgery.

To understand the anatomy and the surgical approaches to the petrous, 19 adult human skulls (38 specimens) were studied. The foramen spinosum, groove for greater petrosal nerve, superior and posterior margins of the internal auditory meatus, fissure for the endolymphatic sac, and anterior margin of the groove for sigmoid sinus were the constant, easily identifiable bony landmarks located strategically; they served as guides during the transpetrosal approach. Measurements between various points helped both to understand the local anatomy better and to decide the degree and direction of safe bony removal during transpetrosal surgery.

Adult↗
PubMed1994

Altered frequency-dependent characteristics of the cardiac baroreflex in essential hypertension.

Blood pressure (BP) oscillations contain rhythmic components of low-, mid-, and high-frequency bands (0.02-0.06, 0.07-0.14, 0.15-0.40 Hz, respectively). Baroreceptors may sense each BP oscillation and induce a buffer reaction. To clarify the frequency-dependent baroreflex response and its possible alteration in patients with high BP, both BP (Finapres) and the RR interval (electrocardiogram) were continuously monitored in untreated patients with essential hypertension (EH) (n = 52) and normotensive subjects (NT) (n = 43). The magnitude and phase response of cardiac beats to BP oscillations were examined by transfer function analysis. Spontaneous baroreflex sensitivity was assessed by linear regression analysis of the BP and RR oscillations. The heart rate responded linearly to BP oscillations in more subjects at mid- and high-frequency bands (83% or more) than at the low-frequency band (60% or less). The phase was approximately zero at the high-frequency band and was consistently negative at the mid- and low-frequency bands. In general, all frequency gains were significantly and positively correlated with spontaneous baroreflex sensitivity. Each frequency gain was smaller in EH patients than in NT subjects, the high-frequency gain more so than the gains at the lower frequencies. In seven young EH patients, treatment with a beta 1-adrenoceptor selective blocker normalized the high-frequency gain and tended to increase the gains at the lower frequencies. These results suggest that the spontaneous baroreflex modulates RR oscillations over a broad frequency range from 0.02 to 0.40 Hz; the effect was most marked at frequencies higher than 0.07 Hz. Furthermore the frequency-dependent characteristics of the cardiac baroreflex were altered in essential hypertension partly because of an increased beta-adrenergic activity.

Adrenergic beta-1 Receptor Antagonists↗
PubMed1994

Distinct regulation of sodium channel types I, II and III following nerve transection.

We investigated mRNA expression of type I, II and III voltage-sensitive sodium channels in motoneurons following facial nerve transection by means of in situ hybridization histochemistry. Type I mRNA expression decreased markedly after nerve transection, while that of type III increased. Type II expression underwent no detectable change following nerve transection. These results suggest that type III sodium channels may be involved in regeneration and plasticity.

Animals↗
PubMed1994

Localization of 63-kDa calmodulin-stimulated phosphodiesterase mRNA in the rat brain by in situ hybridization histochemistry.

We studied the localization of 63-kDa Ca2+/calmodulin stimulated phosphodiesterase mRNA in the rat brain by in situ hybridization histochemistry using an oligonucleotides probe specific to this enzyme. The signals were especially concentrated in several brain regions such as the olfactory tubercle, accumbens nucleus, caudate putamen, fundus striati, dentate gyrus of the hippocampus, pontine nuclei and dorsal tegmental nucleus. These results suggest that in the neuronal groups containing the strong signals this enzyme is involved in calcium-dependent signal transduction system coupled to cyclic nucleotides messenger systems.

Animals↗
PubMed1994

Effects of bromocriptine in Huntington chorea. Case report.

1. The effects of bromocriptine (BC) on choreiform movement were compared with those of bromperidol (BP) and fluphenazine (FLZ) in a patient with Huntington disease. The patient (male, 42 years old) was treated with BP (15 mg/day, 4 weeks), FLZ (3 mg/day, 4 weeks), low dose of BC (5 mg/day, 4 weeks) and relatively high dose of BC (10 mg/day, 8 weeks). The CSF content of homovanilic acid (HVA) was assayed at last day of the each drug trial. The efficacy of the drugs was evaluated by electromyography. 2. Although BP and FLZ did not succeed to ameliorate the choreiform movement, both low dose and high dose BC showed rapid improvement of the involuntary movement. The CSF HVA concentration was 35.0 ng/ml before beginning treatment. Whereas FLZ and high dose of BC substantially increased the levels of HVA after the dosage (49.3 and 53.1 ng/ml, respectively), moderate increase of HVA (41.5 ng/ml) was observed when the low dose of BC was administered. These observations suggest that increase of CSF HVA might be necessary for clinical improvement of choreiform movement but not correlate with the degree of improvement and dopamine agonists could be useful drug for the treatment of choreiform movement which is refractory to the administration of neuroleptics.

Adult↗
PubMed1994

The effect of age on plasma level of setiptiline maleate in depressed patients.

1. Setiptiline maleate (SPT) was administered orally to 45 subjects aged 22-86 years and steady state plasma levels were determined by mass fragment chromatography (GC-MF) to examine the effect of aging on those values. 2. There was a significant correlation between the plasma levels and daily dose. However, there was a wide interindividual variability. 3. Dose-corrected plasma level (DC-PL), or values corrected by dividing the plasma level by daily dose/body weight, was used as the systemic drug clearance parameter. 4. DC-PL was compared among 7 age groups of the subjects distributed in 10-year-intervals. DC-PL showed no difference among groups of subjects between the > 29 years bracket to the 70 years bracket, but showed significantly higher values in those in the > 80 bracket compared to all age groups and subjects in the < 79 bracket. 5. There was a significant correlation between the age of patients and DC-PL according to polynomial response curve analysis. Regression analysis yielded the equation y = -52.72 + 7.05 x -0.17 x2 + 0.01 x3 (n = 45, r = 0.49, p < 0.01).

Adult↗
PubMed1994

Expression of pi-glutathione S-transferase gene (GSTP1) in gastric cancer: lack of correlation with resistance against cis-diamminedichloroplatinum (II).

Class pi-glutathione S-transferase (GSTP-1) is one of several factors proposed to affect drug sensitivity to cisdiamminedichloroplatinum (II) (CDDP). It has also been investigated as a potential marker for the serodiagnosis of various types of cancers. In this study, attempts were made to quantify mRNA levels of the enzyme in healthy and cancerous gastric mucosa specimens, and to evaluate their significance in inherent drug resistance to CDDP. Thirty gastric cancer specimens were analysed by northern blotting with radiolabelled GSTP1 cDNA. Of these, the chemosensitivities of 22 specimens were evaluated by the succinic dehydrogenase inhibition (SDI) test. GSTP-1 mRNA was detected in all the specimens, with slightly increased, but non-significant expression in the neoplasms. Comparison of these drug sensitivities with results of northern blotting analysis showed no inverse correlation, as was expected from the widely investigated role of the enzyme in drug resistance.

Blotting, Northern↗
PubMed1994

Neuroblastoma of parotid gland: report of a case and immunohistochemical characteristics.

A case of a parotid mass in a 2-year-old boy, postoperatively diagnosed as neuroblastoma, a rare tumour not previously reported in the parotid gland is presented. The neoplasm developed within the parotid gland as a painless mass without regional lymphadenopathy. Histopathologically, the tumour showed primitive nerve cells-neuroblasts-with round or oval dark basophilic nuclei and scanty cytoplasm. The cells were arranged in circular rosettes around an eosinophilic mass consisting of very fine filaments originating in the tumour cells or papillary configuration and sometimes scattered in the poorly developed stroma. Immunohistochemical evaluation of the tumour showed a positive immunoreactivity for vimentin, alpha and beta subunits of S-100 protein, neurone-specific enolase (NSE), substance P, met-enkephalin and chromogranin but cytokeratins, desmin, actin, myosin, glial fibrillary acidic protein (GFAP) and calcitonin gene related peptide (CGRP) were negative. The histopathological and immunohistochemical findings conclude a diagnosis of neuroblastoma of the parotid gland.

Child, Preschool↗
PubMed1994

Enhanced tenascin immunoreactivity in leukoplakia and squamous cell carcinoma of the oral cavity: an immunohistochemical study.

Tenascin is an extracellular matrix glycoprotein that shows a site restricted expression especially in areas of cell proliferation, cell motility, and tissue modeling at the epithelial-mesenchymal junction during embryogenesis. Tissue specimens obtained from surgery and/or biopsy for oral leukoplakia (n = 22) and squamous cells carcinoma (n = 36) were examined for the presence of tenascin by using monoclonal antibody. In normal tissue specimens (n = 5), tenascin immunoreaction appeared as a linear continuous lining at the immediate vicinity of basement membrane (n = 3). Hyperplastic epithelia in leuoplakia showed a distinct increase in tenascin immunoreactivity in the submucosa correlating with the degree of hyperplasis and/or dysplasia. In squamous cell carcinoma (SCC), the reactivity was most intense extending deeply into the underlying stroma with marked reaction around large tumour cell nests and the infiltrating tumour margin. The connective tissue stroma, however, in undifferentiated carcinoma showed traces of immunoreactivity. Positive immunoreactivity was seen around metastatic squamous cell carcinoma masses in regional lymph nodes. The stromal tissues infiltrated by inflammatory cells were usually unreactive while those with desmoplastic changes were positive for tenascin. The authors conclude that an enhanced expression of tenascin may play an important role during active phases of tumour cell proliferation and stromal changes in the premalignant and malignant lesions of the oral mucosa.

Carcinoma, Squamous Cell↗