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Biomedical subjects

H Taira

Publications and source records attributed to H Taira.

At least 55 records · Page 3Linked to original sources
PubMed1993

Analysis of phosphorylation of wheat elongation factor 1 beta and beta' by casein kinase II.

The purified casein kinase II (CK II) from Arabidopsis thaliana phosphorylates wheat elongation factor 1 beta (EF-1 beta), but not elongation factor 1 beta' (EF-1 beta'), which lacks a serine residue in the conserved phosphorylation site. Both EF-1 beta and beta' subunits, with similar functions, seem to undergo different regulation despite the partial amino acid sequence of EF-1 beta being similar to that of EF-1 beta'.

Amino Acid Sequence↗
PubMed1992

Cloning and sequencing of the cDNA encoding rice elongation factor 1 beta'.

A cDNA clone coding for rice elongation factor 1 beta' (EF-1 beta') was isolated from a rice anther cDNA library. The clone, named RB', was 980 bp long and contained a single open reading frame coding for 223 amino acids; the first 31 amino acids, except for the first methionine, which is absent in the mature protein, are identical to those of the purified protein determined with a protein sequencer. The amino acid sequence of rice EF-1 beta' shows homology to the C-terminal half of Artemia salina EF-1 beta (59%) and human EF-1 beta (63%), but might not have a phosphorylation site for casein kinase II which has been conserved in Artemia saline EF-1 beta and EF-1 delta, human EF-1 beta and silkworm EF-1 beta'.

Amino Acid Sequence↗
PubMed1992

[Sugi (Cryptomeria japonica D. Don) pollen scattering out of season].

We investigated the preseasonal blooming conditions of the male flowers of Sugi (Cryptomeria japonica D. Don) which flowers commonly in spring. We also investigated atmospheric Sugi pollen during all seasons for three years from 1988 through 1991 to determine pollen scattering in autumn. Our results showed that a discernible amount of pollen is scattered from the middle of October into December; this scattering would be expected in the following spring. Almost none of the buds of the flowers collected in October and in November bloomed when incubated at temperatures ranging from 6 degrees C to 20 degrees C after low-temperature treatment to arise the blooms from dormancy. However, small numbers of the male flowers from various Sugi trees bloomed and scattered pollen under these conditions. We found that the ratio of the blooming male flowers was one-third or less per blossom. The ratio changed depending on the growth stage and incubation conditions. In conclusion, the density of airborne Sugi pollen scattering is not high in autumn in general. However, the density would be expected to be high enough to induce allergic reactions and clinical symptoms in some areas of Sugi growth, especially in a good harvest year.

Air↗
PubMed1992

[Prediction of the day on which sugi (Cryptomeria japonica D. Don) pollen scattering will begin--from view point of plant physiology].

We investigated the cumulative thermal constant and threshold temperature for the development of sugi (Cryptomeria japonica D. Don) male flowers to predict the day on which pollen scattering would begin. The threshold temperature for the development of sugi male flowers was 0.17 degrees C and the cumulative thermal constant was 184 degrees C/day. Using these results, we determined how long it would take for sugi to be awaken from dormancy and we investigated the meteorological date. Reactivation from dormancy requires 3 days, where the minimum temperature is less than 3 degrees C with a mean temperature of less than 6 degrees C; the following day must have a maximum temperature of more than 10 degrees C, and these conditions must be maintained for at least 2 days. From these results, it is not difficult to predict the day on which sugi pollen scattering will begin.

Forecasting↗
PubMed1991

[Relationships between the pollen productions in sugi (Cryptomeria japonica D. Don) forests and the patterns of atmospheric scattering pollens in Toyama Prefecture--the example of Toyama Prefecture in 1990].

We investigated the geographical distribution of sugi (Cryptomeria Japonica D. Don) forests and the male flower-setting conditions of them in Toyama prefecture. The relationships between our findings and the atmospheric pollen scattering patterns were also studied. The results were as follows. 1. Atmospheric pollen counts and their scattering patterns were fairly easily explained by the geographical distribution of 30-year old sugi forests, the male flower-setting conditions and the first day pollen was scattered in those forests. 2. Total atmospheric counts and maximum sugi pollen peaks were significantly affected by the proximity of sugi forests at altitudes of less than 200 meter. 3. In the atmospheric pollen survey, the detected pollen counts decreased according to the distance between the observation sites and the sugi forests. And few pollen grains were observed from natural sugi forests growing at distances of 20 km to 30 km from the observation sites.

Air↗
PubMed1990

Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells.

To examine the properties and the role of the fusion protein (F) of Sendai virus at the molecular level, a plasmid, pUC-F, was constructed by inserting cDNA for the F protein into a pUC vector. Upon induction of E. coli cells transformed with pUC-F, a new protein was obtained, which was identified as Fo on Western blot analysis. The cDNA fragment for the F gene was excised from pUC-F and inserted into an eucaryotic expression vector, pSVL, to yield pSVL-F. COS-1 cells transfected with pSVL-F gave a band on SDS-gel electrophoresis which corresponded to the size of the Fo proteins.

Escherichia coli↗
PubMed1990

Association of the Sendai virus C protein with nucleocapsids.

The subcellular localization of the nonstructural protein C of Sendai virus was investigated by means of indirect immunofluorescence microscopy of Sendai virus-infected cells, using an antiserum specific for C protein. In infected cells, C protein was detected exclusively in the cytoplasm as granular fluorescence, which coincided very well with the distribution of nucleocapsid protein NP and phosphoprotein P, which were also detected with specific antisera. This suggested that these proteins are present together in inclusions, probably forming nucleocapsids. In contrast, when the NP and C proteins were individually expressed in COS cells by transfection with expression plasmids containing cDNA for these proteins, their distribution patterns in the cytoplasm were found to be quite different from each other. Protein-blot analyses of purified virions revealed the presence of a significant amount of the C protein in virions, which indicated that C protein is integrated into virions. Under conditions in which most of the envelope-associated proteins, such as HN, F, and M, were removed from the virions by a detergent, the C protein remained tightly associated with the nucleocapsids--about 40 molecules per nucleocapsid.

Animals↗
PubMed1990

[A case with reconstruction of the floor of the oral cavity and mandibular region with the D-P flap after resection of giant keloid and radiation scar of the mandibular].

The floor of the oral cavity and mandibular of a 27-year-old female was reconstructed with the D-P flap after resection of a giant keloid in the submandibular region and a radiation scar of the mandibular. The mandibular was reconstructed with a transplantation graft of the iliac bone and the functional properties and appearance were good.

Adult↗
PubMed1989

Rabbit brain glucose transporter responds to insulin when expressed in insulin-sensitive Chinese hamster ovary cells.

Transfection of Chinese hamster ovary cells with the expression vector containing rabbit brain HepG2-type glucose transporter cDNA resulted in a dramatic over-expression (approximately 10-fold) of glucose transporter as assessed by either immunoblotting with antipeptide antibody against rabbit brain glucose transporter or photoaffinity labeling with [3H]cytochalasin B. 2-Deoxyglucose uptake was also increased 4-fold in the transfected cells, while no increase in transport activity or transporter amount was observed in cells that were transfected with the expression vector alone without glucose transporter cDNA. Significantly, insulin (10(-7) M) increased 2-deoxyglucose uptake in both control and transfected cells, but the increased amount of the transported 2-deoxyglucose by insulin in the transfected cells was 4.2-fold greater than that in control cells, indicating that the expressed rabbit brain HepG2-type glucose transporter responded to insulin. In addition, we have recently demonstrated that the HepG2-type glucose transporter exists in rat adipocytes and responds to insulin in a fashion similar to a majority of other types of glucose transporters (Oka, Y., Asano, T., Shibasaki, Y., Kasuga, M., Kanazawa, Y., and Takaku, F. (1988) J. Biol. Chem. 263, 13432-13439). In contrast, insulin did not stimulate glucose transport activity in HepG2 cells or IM-9 lymphocytes that have a significant amount of the HepG2-type glucose transporter. Thus, the results in this study further support the notion that insulin regulation of glucose transport activity depends on a tissue-specific signaling mechanism.

Affinity Labels↗
PubMed1989

[Report of a case of Herpes zoster].

Herpes Zoster is a viral disease of the skin and mucosa characterized by grouped vesicula eruptions and neuralgic pain along a peripheral nerve. A case of Herpes Zoster in the right region along the second and third trigeminal nerve branches of a 24-year-old male was reported. The first disorder appeared as a grouped vesicular eruption in the center of the lower lip. This was followed by a cutaneous lesion in the area of the right second trigeminal nerve branch. From the first day of hospitalization, the patient began receiving a daily dose of 2500mg of immunoglobulin. The administration of immunoglobulin, cured the oral and cutaneous lesion.

Adult↗
PubMed1987

Effect of microsomal leucine aminopeptidase from human placenta (microsomal P-LAP) on pressor response to infused angiotensin II (A-II) in rat.

The role of microsomal placental leucine aminopeptidase (microsomal P-LAP) in the decreased pressor responsiveness to angiotensin II (A-II) in pregnancy was studied. Appreciable amounts of microsomal P-LAP activity were found in rat placenta. The similar dose to the endogenous activity, of human microsomal P-LAP exogenously administered to rats, resulted in significant decrease in the response to A-II. Bestatin, an inhibitor of the microsomal leucine aminopeptidase administered to pregnant rats, enhanced the A-II response. Therefore our present study suggests such refractoriness in response to A-II in pregnancy is due to increased inactivation by the microsomal P-LAP. It was also suggested that prostaglandins were not involved in such refractoriness by the experiments with indomethacin.

Angiotensin II↗
PubMed1987

The target reaction in the antiviral action of mouse interferon against vesicular stomatitis virus multiplication.

Treatment of mouse L929 cells with mouse interferon (IFN) lowered the yield of vesicular stomatitis virus (VSV) in a dose-dependent manner. Accumulation of viral proteins was severely inhibited in IFN-treated cells, whereas cellular protein synthesis was not, indicating that the virus-induced shutoff of cellular protein synthesis was prevented by IFN. In order to identify the major target of IFN action precisely, the effect of IFN treatment on the synthesis of viral RNAs and proteins at various stages during the course of viral replication was examined. Accumulation of viral RNAs late in infection was inhibited, as was the case with viral proteins, but the synthesis of leader RNA and mRNAs early in infection was not significantly inhibited by treatment with a moderate dose of IFN. On the other hand, viral protein synthesis at an early stage of infection was strongly inhibited by IFN. The results indicate that the major target reaction of antiviral action of IFN against VSV multiplication is the translation of viral mRNA.

Animals↗
PubMed1987

Synthesis and biological activities of beta-alanyltyrosine derivative of 2',5'-oligoadenylate, and its use in radiobinding assay for 2',5-oligoadenylate.

beta-Alanyltyrosine derivative of 2',5'-tetraadenylate 5'-triphosphate, pppA2'p5'A2'-p5'A2'p5'A-beta-Ala-Tyr was prepared by coupling of periodate-oxidized pppA2'p5'-A2'p5'A2'p5'A with beta-alanyltyrosine methyl ester, followed by reduction with sodium cyanoborohydride. Its stability to 2',5'-phosphodiesterase and phosphatase was investigated in mouse L cell extract. The 5'-triphosphate of the compound was cleaved gradually to form the 5'-dephosphorylated derivative, A2'p5'A2'p5'A2'p5'A-beta-Ala-Tyr, followed by slow degradation of the 2',5'-phosphodiester bond. On the other hand, pppA2'p5'A2'p5'A2'p5'A was hydrolyzed very quickly under the same conditions. The tetramer derivative bound tightly to the 2',5'-oligoadenylate-dependent endoribonuclease in rabbit reticulocyte lysate or mouse L cell extract and inhibited protein synthesis of mouse L cells more effectively than the unmodified 2',5'-tetraadenylate 5'-triphosphate. The corresponding trimer derivative had slightly weaker activities than the unmodified trimer for binding to the endoribonuclease and for inhibition of protein synthesis. The compound, pppA2'p5'A2'p5'-A2'p5'A-beta-Ala-Tyr, was iodinated easily at the tyrosine residue with 125I, giving a high-specific-radioactivity derivative which was used as a radio-labeled probe in a radiobinding assay for 2',5'-oligoadenylate.

Adenine Nucleotides↗