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Biomedical subjects

H Taguchi

Publications and source records attributed to H Taguchi.

At least 469 records · Page 26Linked to original sources
PubMed1984

Ultrastructural study of type C virus particles in phytohemagglutinin-stimulated lymphocytes from healthy adults seropositive to adult T-cell leukemia-associated antigens.

PHA-stimulated peripheral lymphocytes from six healthy adults consisting of four family members of an adult T-cell leukemia (ATL) patient and two blood bank donors, seropositive to ATL-associated antigens (ATLA), were all positive for the expression of ATLA and ATL-associated virus (ATLV). These results revealed that anti-ATLA-positive persons were healthy carriers of ATLV and that the numbers of ATLV observed in each culture were more proportional to the percentage of ATLA-positive lymphocytes than anti-ATLA titers of each person. Virus particles detected were C-type in morphology, and essentially similar to those observed in MT-2 and other ATL-related cells, but rather uniform in size, mostly 120-130 nm in diameter. Furthermore, it is of interest that tubuloreticular structures and striated fusiform structures, which were found in fresh or short term cultured ATL cells, were observed in some cultures of lymphocytes from anti-ATLA-positive healthy persons.

Adolescent↗
PubMed1984

An atypical von Willebrand's disease with hyperreactivity of platelet aggregation.

Two family members (daughter and mother) with a bleeding disorder showed prolonged bleeding time and activated partial thromboplastin time associated with decreased plasma levels of factor VIII procoagulant activity, factor VIII-related antigen, and factor VIII-ristocetin cofactor activity. The ristocetin-induced platelet agglutination (RIPA) was enhanced, ADP-, collagen- and Ca ionophore-induced platelet aggregation were also increased at low concentrations of these compounds. In the mother, spontaneous platelet aggregation (SPA) was also observed. Contrary to type II B von Willebrand's disease (vWd), pseudo-vWd and platelet-type vWd, the patients did not show any increased binding of factor VIII/vWf to platelets in the presence of ristocetin. The RIPA in normal controls were inhibited by addition of antifactor VIII antiserum to the platelet-rich plasma, but not in cases 1 and 2. In this atypical vWd, therefore, the hyperreactivity of platelet aggregation may be due to an intrinsic abnormality of the platelets, but not to any enhanced interaction between plasma factor VIII and the platelets.

Adult↗
PubMed1984

Infectious transmission of human T-cell leukemia virus to animals.

An human T-cell leukemia virus (HTLV)-producing cell line (Ra-1) was established from rabbit lymphocytes by co-cultivation with lethally irradiated MT-2 cells. Ra-1 cells were inoculated intravenously into a Japanese monkey and rabbits. All animals responded with the production of antibodies to HTLV. Lymphocytes from the seroconverted animals were grown in the presence of T-cell growth factor (TCGF) or co-cultured with lymphocytes from seronegative healthy persons. The TCGF-grown cells, which were chromosomally of the recipient type, expressed HTLV antigens and particles. The co-cultures gave rise to human T-cell lines which also harbored HTLV antigens and particles. Blood transfusion from the infected rabbits resulted in the seroconversion of the recipient rabbits. HTLV-producing lymphoid cell lines were established from some of the transfused rabbits. The recipient origin of these cell lines was determined by chromosome analysis. It was possible to serially transmit HTLV by blood transfusion in rabbits. Thus, these animals offer promise as a laboratory model for HTLV infection.

Animals↗
PubMed1984

Effects of leucovorin and methylcobalamin with N2O anesthesia.

Results of the deoxyuridine suppression test, a good marker for defining biochemical megaloblastosis caused by deficiency of folate and vitamin B12, became abnormal (greater than 10%) after 6 hours of administration of nitrous oxide anesthesia. 5-Formyltetrahydrofolate and methylcobalamin administration during nitrous oxide anesthesia have no remarkable effect on the correction of deoxyuridine suppression test values. On the other hand, 5-formyltetrahydrofolate and methylcobalamin administrated at the end of nitrous oxide anesthesia corrected the abnormal deoxyuridine suppression test values nearly to normal range within 1 hour.

Bone Marrow↗
PubMed1984

Monoclonal gammopathy in adult T-cell leukemia/lymphoma: a report of three cases.

Adult T-cell leukemia/lymphoma (ATL/L) is clustered in southwestern Japan, especially in the Nagasaki and Kagoshima areas. It was shown that this clustering correlated with the presence of antibodies to antigens of a new C-type RNA leukemia virus. Surface marker analysis of neoplastic T-cells of ATL/L patients shows the helper/inducer phenotype (Leu-1+, Leu-2a- and Leu-3a+). On the other hand, the association between T-cell malignancy including ATL/L and monoclonal gammopathy is very rare. Three unique cases of ATL/L with monoclonal gammopathy are reported. Yet the meaning of an M-component in T-lymphocytic proliferation remains uncertain. The relationship between the leukemia virus, ATL/L-cells and monoclonal gammopathy is discussed.

Aged↗
PubMed1984

[Clinical study of eosinophilic cystitis. I. On the definition of eosinophilic cystitis. I-1. Histological observation of eosinophilic infiltration].

Eosinophilic cystitis is a disease accompanied by prominent increase in the number of eosinophils in the vesical wall, but whether it is diagnosable from the number of eosinophils has not been clear. We made histological observations in 5 cases thought to have elevated eosinophil count and 13 control cases. We counted the eosinophils in the 5 fields we considered to be the place eosinophils were most abundant under 200 times magnification, and an average of 20 to 50 eosinophils were found in each field. The ratio of eosinophils in the same field to all round cell infiltration was from 36 to 85%. In 2 of the 5 cases, more than 20 eosinophils were found in some fields and the ratio to all round cell infiltration was under 5%. In the remaining one case from 5 to 20 eosinophils were found in some fields, but the ratio to round cell infiltration was over 20%. On the other hand, in the 13 control cases, the average number of eosinophils was one per field and the ratio to all round cell infiltration was under 5% in the 5 fields we considered to be the place eosinophils were most abundant. Less than 5 eosinophils were found in any of the fields and the ratio to all round cell was under 5%. From these results, the 5 cases thought to have elevated eosinophil count were diagnosed to be eosinophilic cystitis.

Cystitis↗